Ökse Otu (Viscum album ssp. austriacum) Yaprak ve Tomurcuk Ekstraklarının Antioksidan Kapasitelerinin Belirlenmesi

In this study, it was determined that total phenolic and flavonoid substance amounts and antioxidant capacity of methanol extracts obtained from leaves (Y) and buds (T) of mistletoe (V. album ssp. Austriacum) collected from Northeast Anatolian Yellow Pine forests (Pinus sylvestris L). The amount of total phenolic substance was determined as 19.55 ±4.68 mg GAE/g in Y and 16.88 ±2.77 mg GAE/g in T, while amount of total flavonoid substance 17.56 ±2.53 mg QE/g in Y and 17.17 ±3.29 mg QE/g in T. IC50 values were calculated as 476.26 ±22.54 μg/mL in Y and 778.57±25.61 μg/mL in T. In addition, the reducing antioxidant powers of Fe3+-Fe2+ were calculated as 303.00 ±1.73 μg AAE/mL in Y and 307.01±1.05 μg AAE/mL in T, equivalent to ascorbic acid. As a result, it was seen that mistletoe which is considered harmful for yellow pines, has antioxidant properties. It has been concluded that the evaluation of mistletoe collected during the struggle with mistletoe as a natural antioxidant source may contribute economically.

Fractionation of Sugarcane Extracts and Determination of Phenolic Compounds and their Antioxidant Activity

Crude ethanolic extracts of four cultivars of sugarcane (Saccharum officinarum L.) were firstly prepared and then fractionated using silica gel column chromatography before the determination of their oxidative substances. The fractionated extracts were then tested for antioxidant activity by various assays. Finally, high-performance liquid chromatography (HPLC) was applied for the quantitative determination of the individual phenolic compounds. Sub-fraction 2 has the highest phytochemical contents as well as antioxidant activity. All tested phytochemicals had positively correlated to antioxidant activity. HPLC analysis showed that the phytochemicals in the fractionated extracts varied by the sugarcane cultivars. The main flavonoid substances found in the fractionated extracts were epicatechin, catechin, quercetin, resveratrol, myricetin and rutin while gallic acid was the main phenolic substance. The obtained information is useful for further studies and applications.

Phenolic substances and cyanogenesis in galled and non-galled tissue of the fern species Microgramma vacciniifolia

Galls, neo-formed plant structures that can occur in different organs, are generated by species-specific interaction with an inducing organism. Inducers manipulate the metabolism of its host. Microgramma vacciniifolia (Langsd. & Fisch.) Copel. is a Neotropical epiphytic fern that hosted two stem galls, one induced by a midge species (Diptera) and other by a micromoth species (Lepidoptera). The aim of this study was to evaluate the impact of these two gall-inducing insects on the biochemistry of phenolic acids and the cyanogenesis in galls, stems and leaves of M. vacciniifolia. High performance liquid chromatography (HPLC) indicated a total of 14 phenol derivatives, including caffeic and coumaric acid. Principal Coordinates Analysis (PCoA) of the phenolic substances indicated three groups consisting (1) non-galled stems and micromoth-induced galls; (2) midge-induced galls; (3) midge-induced galls with parasitoids. Regarding the frequency of cyanogenesis assessed by the picrate paper test, the chi-squared test showed significant difference between fertile leaves (8.3%), sterile leaves (27.7%), non-galled stems (0%) and galls. Among galls, only the midge-induced galls analyzed were cyanogenic (15%). Our results indicated that the different gall-inducers (midge and micromoth) promote species-specific alterations to the phenolic substance composition of the host fern.

Phytochemicals and Aroma Compounds Content of Crabapple (Malus tribolata C.K. Schneid.) Genotypes in Kahramanmaraş Province

Crabapple (Malus tribolata C.K. Schneid.), which one of the rare wild fruit trees in Turkey. This study was carried out to determine the aroma compounds, phenolic compound, antioxidant capacity and some fruit properties of fruits obtained from 2 different crabapple genotypes. The amount of phenolic substance was done by Folin-Ciocalteu method; antioxidant analysis was done using DPPH technique. With respect to antioxidant capacities, the highest value (70.12%) was determined in 46 EL 01 genotype. We found that 46 EL 01 genotype had the highest total phenolic content (839.13 mg/100 g). Determination of volatile compounds that play a major role in fruit quality using the HS-SPME/GC/MS technique, total of 37 aroma compound, namely 7 alcohols, 2 terpenes, 5 aldehydes, 17 esters, 1 ketone, 3 acids and 2 other compounds, were found in two different crabapple genotypes. Total aroma compounds in 46 EL 01 and 46 EL 02 genotypes were calculated as 101.78 μg/L and 102.26 μg/L, respectively. As a result, it has been determined that crabapple, which is a wild fruit, has high phenolic and antioxidant contents and also has many aroma compounds.

Ocimum basilicum L. Bitkisinde Rosmarinik Asit ve Antioksidan Bileşenler İçin Yeşil Ekstraksiyon Koşullarının Deneysel Tasarımı ve Çok Yanıtlı Optimizasyonu

The main purpose of this study is to develop and optimize a method based on the hydroalcoholic solvent extraction of rosmarinic acid (RA) which is an important active component in the polyphenolic profile of the plant and, stands out with its antioxidant, antibacterial, and antiviral properties, and other antioxidant components. Soft computing-based chemometric studies are needed to model and optimize complex processes such as extraction. In this study, the extraction conditions of bioactive RA from Ocimum bacilicum L., a valuable medicinal aromatic plant source, with green and simple technique, were modeled and optimized using chemometric methods. Additionally, it was also aimed to evaluate the potential of RA in O. basilicum with the amount of Total phenolic substance (TFM) and Total antioxidant activity (TAA). In the study, RSM-CCD/small factorial design was used, multi-response optimization was solved with the help of Pareto solutions, and the Desirability function was used to determine the optimum input variable values. As a result, optimum conditions of O. basilicum L. extraction, 47.7% EtOH concentration, 30.0 °C temperature, 77.6 min extraction time, and 10 mL/g solvent/solid ratio were found. Experimental results obtained under optimum conditions were 171.46±1.87 mg GAE/g, 4.76±0.32 mg Trolox/mL, and 8.93±0.65 mg/g for TPC, TAA and RA, respectively. The predicted results from the mathematical model were (172.26 mg GAE/g), (4.13 mg Trolox/mL) and (8.89 mg/g), respectively.

Comparison of Fatty Acid Composition and Antioxidant Contents of Tribulus Terrestris L. Collected from Different Localities

For a long time, many cultures around the world have used Tribulus terrestris L. in the prevention and treatment of various diseases. In this study, the antioxidant activity and total phenolic and flavonoid content of extracts obtained with various solvents from T. terrestris plant collected from different localities in Kahramanmaraş were investigated. In addition, the fixed oil content of the extracts was examined by GC-MS analysis and as a result, 26 different fatty acids were determined. The main fatty acid components of plant extracts are linoleic acid, oleic acid and palmitic acid. The total phenolic substance value of plant extracts varies between 2.20-18-77 mg g-1, total flavonoid amount varies between 0.06-0.50 mg g-1, FRAP value varies between 6.16-23.50 µg g-1 and DPPH value varies between 1.54-10.54 µg mL-1. It was observed that the solvents used in extraction affected the bioactivity values rather than the locations. Although the absorbance values of the extracts obtained with hexane were high, low extract yield affected the results. The highest values in all characters examined were obtained from ethanolic extracts.

Is Juglone the Only Naphthoquinone in Juglans regia L. with Allelopathic Effects?

We investigated whether juglone is the only allelochemical in a leaf extract from the walnut (Juglans regia L.). This was achieved through comparisons of the effects of pure juglone (1 mM, 100 μM, 10 μM control juglone) and J. regia leaf extract (prepared as 1 mM, 100 μM leaf juglone) on seed germination, seedling growth, and secondary metabolism of the selected crop vegetables. Two control treatments were also applied, as extraction medium and water. For inhibition of seed germination, S. lycopersicum, B. rapa var. japonica, and V. locusta were more sensitive to 1 mM leaf juglone, and L. sativa was more sensitive to 1 mM control juglone. This suggests that this walnut leaf extract contains specific phenolic substance(s) that can stimulate seed germination in some species and inhibit it in others. Seedling length was more sensitive to 1 mM leaf juglone than 1 mM control juglone, with selective strong inhibition of root length versus shoot length by 1 mM control juglone. Juglone also had significant effects on the secondary metabolism of L. sativa, in particular for seedlings treated with 100 μM control juglone, with marked decreases in all secondary metabolites studied. Flavonols constituted the majority of these metabolites in L. culinaris, which showed the least sensitivity to both control juglone and leaf juglone treatments. Thirty compounds were identified and quantified in S. lycopersicum, L. culinaris, and L. sativa, some for the first time in these plants, and all for the first time in the seedlings of these crop vegetables.

Evaluating The Impact of Strigolactone GR24 On Capparis Spinosa L. Callus Production And Phenolic Compound Content

The effect of strigolactones on plants, which has been recently described as a new group of plant hormones, has not been fully characterized. Capparis spinosa L. callus formation using synthetic strigolactone GR24 (0.1 and 0.2 μM) alone or in combination with 1-naphthalene acetic acid (NAA) (2 mg / L) and 6-benzylaminopurine (BAP) (1 mg / L) and its effect on phenolic substance production were evaluated. 2 mg/L NAA+1 mg/L BAP+0.1 μM GR24 was the medium with the highest callus formation (60.3%) and callus fresh weight (120.8 mg). In the phytochemical analysis, the highest total flavonoid and phenolic substance and the highest rutin, quercetin, chlorogenic acid content were found in this application and their amounts increased at various rates compared to the control. Aromatic substances in caper calluses were grouped as sulfur compounds (66.97% -87.53%), aldehydes (4.88% -7.90%), ketones (0.34% -19.3%), hydrocarbons and derivatives (0.56%-5.8%), alcohols (% 1.62-6.08%), others (0.61% -2.37%) and their amounts varied at various hormone applications. When 0.1 μM GR24 was applied alone, the total sulfur compound in callus samples was 87.53% and the dominant substance was found to be methyl isothiocyanate.

Lonicera iberica M. Bieb.: Investigation Antioxidant Activity and Bioactive Chemicals

In this study, it was investigated the total amounts of phenolic and flavonoid substances and antioxidant activities in different solvent extracts of Lonicera iberica M. Bieb. wild fruit. Total phenolic contents of the extracts were determined as equivalent to gallic acid using Folin-Ciocalteu reagent, and total flavonoid contents as equivalent to quercetin by aluminium nitrate method. In addition, the antioxidant properties of the extracts were determined using free radical scavenging (DPPH) and reducing power (FRAP) methods. The amount of total phenolic substance of L. iberica fruits in hexane and methanol extracts was calculated as 30.96 ± 0.67 mg of GAE / g and 23.70 ± 1.56 mg of GAE / g, respectively. In addition, the amount of total flavonoid substance was calculated as 46.50 ± 8.54 mg of QE / g and 42.09 ± 2.58 mg of QE / g, respectively. It was determined that DPPH radical scavenging activity correlated with total phenolic and flavonoid amount of substance, and L. iberica had a strong antioxidant effect. It is predicted that our study will shed light on new researches, since it is the first study done with L. Iberica fruits in this field.

Farklı Maviyemiş Çeşitlerinden (Vaccinum Sp.) Üretilen Reçel ve Marmelatın Bazı Özelliklerinin Belirlenmesi

In this study, seasonal fruits belonging to two different varieties of Blueberry (Brigitta and Darrow) grown in Turkey were processed into jam and marmalade in order to ensure their availability throughout the year. It is aimed to examine the changes in the phytochemical properties of the obtained products during the storage period, such as total phenolic substance, total anthocyanin and antioxidant capacity, which are extremely important in terms of nutrition and health. Total phenolics content (TPC), total antioxidant capacity (TEAC and FRAP), total anthocyanins (TA), polymeric color (PC) and hydroxymethylfurfural (HMF) analyses were performed in fresh currant fruits at the beginning of the storage. Also, the same analyses were carried out on jams and marmalades at the end of 6-month storage period. During storage, the amount of total phenolic compounds, TEAC value, FRAP value and amount of anthocyanin values for jam and marmelade samples varied between respectively are 178.68-518.45 μg GAE/g; 1.16-11.60 μmol TE/g; 1.83-10.33 μmol TE/g; 7.35-298.22 µg cy-3 glu/g. Darrow blueberry samples had the highest values in the all types of blueberry jam and marmelade during storage. At the end of 6 months of storage period, it was observed to jam and marmalade samples of Darrow variety had the highest increase (124% and 73%) in terms of polymeric color value, on the other hand jam and marmalade samples of Brigitta variety had the highest increase (65% and 87%) in terms of HMF value.