scholarly journals PHYTOECDYSTEROIDS OF SERRATULA L. AND KLASEA CASS. GENERA: CHEMODIVERSITY, METHODS OF ISOLATION AND ANALYSIS

2017 ◽  
pp. 123-135
Author(s):  
Даниил (Daniil) Николаевич (Nikolaevich) Оленников (Olennikov) ◽  
Нина (Nina) Игоревна (Igorevna) Кащенко (Kashchenko)
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
High Performance ◽  
Structural Features ◽  
Side Chain ◽  
Individual Species ◽  
Hydroxyl Groups ◽  
Asteraceae Family ◽  
Methods Of Isolation

Serratula L. and Klasea Cass. are two systematically related genera of Asteraceae family containing phytoecdysteroids, a group of natural terpene compounds with various biological activity. Beginning from the 1970s, 76 phytoecdysteroids were isolated and identified in 13 species of Serratula and 5 species of Klasea. This review presented information on the chemodiversity of phytoecdysteroids of Serratula and Klasea genera and their occurrence in individual species. It was shown that the structural features of Serratula and Klasea phytoecdysteroids include the presence of a complete side chain at C-20 atom as well as 5 to 7 hydroxyl groups. Among the species studied, the most investigated were S. coronata, S. tinctoria and S. chinensis with 50, 21 and 19 known compounds, respectively. Also in the review the information on the methods of extraction, isolation and analysis of phytoecdysteroids of the Serratula and Klasea genera was included. The special attention was paid to the data on chromatographic separation of phytoecdysteroids using column, thin-layer and high-performance liquid chromatography on the various sorbents. The information presented in the review demonstrated the perspectiveness of Serratula and Klasea species as sources of phytoecdysteroids.

scholarly journals Transformation of Amoxapine byCunninghamella elegans

2000 ◽  
Vol 66 (8) ◽  
pp. 3646-3649 ◽  
Author(s):  
Joanna D. Moody ◽  
Donglu Zhang ◽  
Thomas M. Heinze ◽  
Carl E. Cerniglia
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
High Performance ◽  
Tricyclic Antidepressant ◽  
Cunninghamella Elegans ◽  
Toxicological Evaluation

ABSTRACT We examined Cunninghamella elegans to determine its ability to transform amoxapine, a tricyclic antidepressant belonging to the dibenzoxazepine class of drugs. Approximately 57% of the exogenous amoxapine was metabolized to three metabolites that were isolated by high-performance liquid chromatography and were identified by nuclear magnetic resonance and mass spectrometry as 7-hydroxyamoxapine (48%),N-formyl-7-hydroxyamoxapine (31%), andN-formylamoxapine (21%). 7-Hydroxyamoxapine, a mammalian metabolite with biological activity, now can be produced in milligram quantities for toxicological evaluation.

The Study of Senkyunolide I Degradation Products by Ultra-High Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry

2014 ◽  
Vol 1033-1034 ◽  
pp. 298-305
Author(s):  
Yao Kun Xiong ◽  
Xiao Lin ◽  
Zhi Yong Liu ◽  
Gen Hua Zhu ◽  
Zhi Hong Yan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Degradation Products ◽  
Time Of Flight ◽  
Bright Light ◽  
Hydroxyl Groups ◽  
Separation And Purification ◽  
Senkyunolide I

Ligusticum chuanxiongHort. (Umbelliferae) has been prescribed widely to treat cardiovascular diseases in China for centuries. Senkyunolide I is a major bioactive component inL. chuanxiongthat shows pharmacological activity against migraines and oxidative damage. Here, we identified the degradation products of senkyunolide I under different conditions (temperature, light alkaline) by ultra-high-performance liquid chromatography–quadrupole time-of-flight–tandem mass spectrometry (UPLC-QTOF-MS) analyses. Senkyunolide I was separated on an ACQUITY UPLC HSS T3 C18 (100 mm × 2.1 mm i.d., 1.8 μm) column using a binary eluent under gradient conditions. Analytes were detected by ESI/QTOF–MS/MS in positive ion mode to obtain MS and MS/MS spectra together with extracted molecular weights. Upon comparison with reference data, we concluded that senkyunolide I undergoes a ring-opening reaction under alkaline conditions (pH >10.0), and dual-key addition or hydration on 3,4-unsaturated bonds and branched terminal hydroxyl groups under high-temperature and aerobic conditions. With auxiliary application1H-NMR, we determined that partial isomerization of senkyunolide I takes place under bright light. In the method detailed here, the advantages of separation and identification of a complex system of small and medium-sized molecules was exploited using UPLC–QTOF–MS, not only to avoid complicated separation and purification, but also to isolate and identify trace amounts of degradation products that were previously difficult to identify.

scholarly journals A Pair of Novel Sulfonyl-Containing N-Acetyldopamine Dimeric Enantiomers From Aspongopus chinensis

2020 ◽  
Vol 15 (3) ◽  
pp. 1934578X2091127
Author(s):  
Li Liao ◽  
Yong-Ming Yan ◽  
Te Xu ◽  
Hou-Lin Xia ◽  
Yong-Xian Cheng
Keyword(s):  
High Performance Liquid Chromatography ◽  
Biological Activity ◽  
Liquid Chromatography ◽  
Renal Fibrosis ◽  
High Performance ◽  
Racemic Mixture ◽  
Spectroscopic Methods ◽  
Liquid Chromatography Separation ◽  
New Compounds ◽  
First Time

A pair of novel sulfonyl-containing N-acetyldopamine dimer enanitomers, (±)-aspongamide E (1), a new ester 2-aminoethyl ( E)-hex-2-enoate (2), along with 3 known compounds (3-5) were isolated from Aspongopus chinensis. Their structures were determined by spectroscopic methods. Compound 1 is a racemic mixture, chiral high-performance liquid chromatography separation followed by electronic circular dichroism calculations assigned the absolute configurations of 2 enantiomers of 1. Compounds 3-5 were isolated from A. chinensis for the first time. The biological activity of the selected new compounds against renal fibrosis was evaluated.

scholarly journals Tryptophan Side-Chain Oxidase Enzyme Suppresses Hepatocellular Carcinoma Growth through Degradation of Tryptophan

2021 ◽  
Vol 22 (22) ◽  
pp. 12428
Author(s):  
Yang Ai ◽  
Ben Wang ◽  
Shuai Xiao ◽  
Sang Luo ◽  
Yefu Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Amino Acid ◽  
High Performance ◽  
De Novo ◽  
Carcinoma Cells ◽  
Side Chain ◽  
Reaction Products ◽  
Hepatocellular Carcinoma Cells

Tryptophan metabolism plays a role in the occurrence and development of hepatocellular carcinoma cells. By degrading certain amino acids, tumor growth can be limited while maintaining the body’s normal nutritional requirements. Tryptophan side-chain oxidase (TSO) enzyme can degrade tryptophan, and its inhibitory effect on hepatocellular carcinoma cells is worthy of further study. To investigate the degradation effect on tryptophan, TSO was isolated and purified from qq Pseudomonas. The reaction products were identified with high performance liquid chromatography (HPLC) and high-performance liquid chromatography tandem mass spectrometry (HPLC-MS). De novo sequencing provided the complete amino acid sequence of TSO. The results of CCK-8, colony formation, transwell, and qPCR confirmed that TSO had inhibitory effects on the proliferation and migration of HCCLM3 (human hepatocarcinoma cell line) and HepG2 cells. The results of flow cytometry confirmed its apoptotic activity. In animal experiments, we found that the tumor-suppressive effect was better in the oncotherapy group than the intraperitoneal injection group. The results of immunohistochemistry also suggested that TSO could inhibit proliferation and promote apoptosis. In conclusion, a specific enzyme that can degrade tryptophan and inhibit the growth of hepatoma cells was authenticated, and its basic information was obtained by extraction/purification and amino acid sequencing.

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