Methods of Isolation and Extraction of Anthocyanin’s from Hibiscus sabdariffa L.: Literature Review

The classic method for extracting anthocyanins from plant tissues is the same as for other phenolics: the tissues are soaked and then extracted with a solvent in a process known as Solid Liquid Extraction. Modern approaches to anthocyanins extraction include;Supercritical fluid extraction, Ultrasound-assisted extraction, Pressurized liquid extraction, Microwave-assisted extraction, Ohmic heating-assisted extraction, Precipitation, and Solid phase extraction. The aim of this article is to review the methods used for Anthocyanins extraction from Hibiscus Sabdariffa.The study for the same has been done at Charak’s Pharmacognosy Laboratory, MIT-WPU School of Pharmacy, Pune. Usually, modern methods seem to have an edge againsttraditional methods in extraction procedures. This article discusses these methodsand tries to identify if it is the case in the case of anthocyanins extraction from Hibiscus sabdariffa L.while individually commenting on the method’s advantages or disadvantages too.

Sertoli cells: immunomodulatory properties, methods of isolation and culture

Due to complications caused by the inevitable use of immunosuppressive drugs in organ and cell transplantation, the use of natural mechanisms of immunological tolerance identified in animal and human organisms arouses interest. It has long been known that there are certain areas in them, including the testis, where immune reactions are virtually impossible. Our review focuses on the role of Sertoli cells that provide testicular immune privilege. Methods of isolation and cultivation of Sertoli cells are described and their potentials in biology and medicine are discussed.

Different Techniques for Rubber Dam Isolation: A Cross-Sectional Study

Objective: To assess knowledge, regarding use of rubber dam during root canal treatment among dentists in Rawalpindi and Islamabad.Study Design: Descriptive cross-sectionPlace and Duration of Study: Operative Department Armed Forces Institute of Dentistry (AFID) Rawalpindi from 1st March 2020 to 31st May 2020. Materials and Methods: A web-based questionnaire, comprising of close-ended questions in the categories of demographics, clinical experience, usage of isolation techniques and knowledge regarding rubber dam (RD) use, procedures, and contraindications was sent through internet to 387 dentists of Rawalpindi and Islamabad. The response rate was 77.5%. Data was analyzed using SPSS version 20, and was displayed as numbers and percentages.Results: 79 out of 300 i.e. 26.33% respondents used rubber dam in restorative and endodontic procedures. 73.3% were using other methods of isolation such as cotton rolls, saliva ejectors and high volume suction. All the respondents were aware about the importance of rubber dam. The most challenging factor for dentists (57.3%) was high influx of patients per day.Conclusion: Rubber dam is not being used commonly by participating dentists of tertiary dental hospitals of Rawalpindi and Islamabad mainly because of high patient turnover per day, combination of cotton rolls and saliva high-volume ejector or gauze is the most common alternative to rubber dam isolation. Rubber dam isolation is highly recommended.

Clusters, Assemblies and Aggregates of Tumor Cells in the Blood of Breast Cancer Patients; Composition, Mode of Action, Detection and Impact on Metastasis and Survival

Circulating tumor cells (CTCs) are gaining momentum as a diagnostic tool and therapeutic target. CTC clusters are more metastatic, but harder to study and characterize, because they are rare and the methods of isolation are mostly focused on single CTCs. This review highlights the recent advances to our understanding of tumor cell clusters with the emphasis on their composition, origin, biology, methods of detection, and impact on metastasis and survival. New approaches to therapy, based on cluster characteristics are also described.

Cannabinoids, Phenolics, Terpenes and Alkaloids of Cannabis

Cannabis sativa is one of the oldest medicinal plants in the world. It was introduced into western medicine during the early 19th century. It contains a complex mixture of secondary metabolites, including cannabinoids and non-cannabinoid-type constituents. More than 500 compounds have been reported from C. sativa, of which 125 cannabinoids have been isolated and/or identified as cannabinoids. Cannabinoids are C21 terpeno-phenolic compounds specific to Cannabis. The non-cannabinoid constituents include: non-cannabinoid phenols, flavonoids, terpenes, alkaloids and others. This review discusses the chemistry of the cannabinoids and major non-cannabinoid constituents (terpenes, non-cannabinoid phenolics, and alkaloids) with special emphasis on their chemical structures, methods of isolation, and identification.

Composition of Collagen-Containing Preparations from Jellyfish Rhopilema asamushi Uchida and Assessment of the Safety of Their External Use

Methods have been developed for obtaining polyfunctional preparations of various degrees of purification from rhopilema jellyfish in the form of collagen and proteoglycane complexes, and their safety for the external use has been assessed. In our study, we used HPLC, SDS-PAGE and IR spectroscopy. The composition (proteins, including collagen, amino acids and carbohydrates) and properties of the obtained preparations were determined. The structure of collagen as a part of the isolated complexes was analyzed, and the degree of preservation of its tertiary structure was shown. Changes in the molecular weight distribution of protein fractions were established for different methods of isolation of the substances. The safety of external use of the obtained drugs was analyzed in terms of acute toxicity, skin irritant and sensitizing action in accordance with the requirements for toxicological studies of ingredients of cosmetics in an experiment on test animals. jellyfish ropilema, collagen, electrophoresis, IR spectroscopy, toxicity, skin irritant effect The authors express their gratitude to P. A. Zadorozhny, Senior Researcher of the Laboratory of Molecular and Elemental Analysis of the Institute of Chemistry, Far Eastern Branch of the Russian Academy of Sciences, for conducting IR spectrometry. The authors are also grateful to O. B. Romanova (Deputy Chief Physician), E. G. Land (Doctor for Sanitary and Hygienic Laboratory Studies), O. A. Stroeva (Biologist of the Vivarium) and G. P. Prikhodko (Veterinarian of the Vivarium), employees of the Center for Hygiene and Epidemiology in Primorsky Krai, for conducting toxicological tests.

Detection of Aflatoxin and Ochratoxin A in Spices by High-Performance Liquid Chromatography

Background. Spices are one of the flavoring components of food in the cooking recipes of different nations that are used daily. However, these ingredients may be contaminated by toxicogenic fungi and subsequent production of mycotoxins that cannot be neutralized through cooking. In the present study, the possible contamination of spices by aflatoxins (AFs) and ochratoxin A (OTA) was investigated from Shiraz, the south part of Iran. Materials and Methods. A total of 80 spice samples including turmeric (n = 20), red pepper (n = 20), black pepper (n = 20), and cinnamon (n = 20) were purchased from markets and cultured on appropriate medium. The isolated fungi were identified. Simultaneously, mycotoxins from spices were extracted with immunoaffinity columns (IAC) and the occurrence of AFs (B1 + B2 + G1 + G2) and OTA was then determined using high-performance liquid chromatography (HPLC) with a fluorescence detector (FD). Result. The results depicted that 40 spice samples were contaminated with AFs and 48 samples with OTA. The highest rate of AFs contamination was related to red pepper, in 80% of which the amount of contamination was excessive than the standard level (>10 μg/kg). All black pepper samples were determined to be contaminated with OTA at over legislation limits of >15 μg/kg. Aspergillus species were the predominant isolated fungi, followed by Penicillium, and Mucor species. Discussion. Regarding the high mycotoxins contamination in spices in the current study, regular effective surveillance and quality control procedures are highly recommended. To achieve this goal, it is necessary to empower food-related laboratories with precise methods of isolation and detection of mycotoxins.

Pathogenic Delivery: The Biological Roles of Cryptococcal Extracellular Vesicles

Extracellular vesicles (EVs) are produced by all domains of life. In fungi, these structures were first described in Cryptococcus neoformans and, since then, they were characterized in several pathogenic and non-pathogenic fungal species. Cryptococcal EVs participate in the export of virulence factors that directly impact the Cryptococcus–host interaction. Our knowledge of the biogenesis and pathogenic roles of Cryptococcus EVs is still limited, but recent methodological and scientific advances have improved our understanding of how cryptococcal EVs participate in both physiological and pathogenic events. In this review, we will discuss the importance of cryptococcal EVs, including early historical studies suggesting their existence in Cryptococcus, their putative mechanisms of biogenesis, methods of isolation, and possible roles in the interaction with host cells.

Influence of methods of isolation of grafted grape cuttings on biological regularities of growth and development at open stratification

В статье дается сравнительный анализ влияния различных способов защиты места соединения подвоя с привоем от подсыхания на прохождение этапов развития привитых черенков винограда во время стратификации. Установлено содержание влаги в привое, подвое и каллюсной ткани при открытом способе стратификации привитых черенков «на воде». Выявлены общие закономерности изменения содержания влаги в привойной части на разных этапах стратификации. Полная изоляция места соединения подвоя с привоем воском RebwachsPROи белой полиэтиленовой пленкой обеспечивает более высокий выход первосортных привитых черенков в сравнении с частичной изоляцией только места соединения подвоя с привоем пленкой. Установлено, что способ изоляции влияет на развитие привитых черенков во время стратификации. Применение воска RebwachsPRO способствует активному распусканию глазка на привое и росту побегов, а применение белой полиэтиленовой пленки, наоборот, сдерживает эти процессы. The article gives a comparative analysis of the influence of various methods of protection of the junction of rootstock and scion from drying on the development stages of grafts during stratification. The moisture content in the rootstock, in grafted cutting and in the callus tissue with open method of stratification of grafts “on the water” was identified. General patterns of changes in moisture content in the scion area at different stages of stratification were revealed. Isolation of junction with RebwachsPRO® wax and white plastic polythene film provided higher output of first-class grafts compared to the partial isolating with the film of the inoculation junction only. It was found that the isolation method effects the development of grafted cuttings during stratification. The use of RebwachsPRO® wax promoted the active opening of eyes on the scion and growth of shoots, while the use of a white polythene film, on the contrary, restrained these processes.