scholarly journals Coronal bacterial leakage in root canals filled with single cone technique and different endodontic sealers

2014 ◽  
Vol 17 (2) ◽  
pp. 57
Author(s):  
Flávia Martins Leal ◽  
Carlos Henrique Ribeiro Camargo ◽  
Marcia Carneiro Valera ◽  
Gleyce Oliveira Silva ◽  
Tatiana Rocha De Oliveira ◽  
...  

<p>Objective: To evaluate coronal bacterial leakage comparing five endodontic sealers (AH Plus, Apexit Plus, Copaifera sp oil, EndoREZ and Polifil), and comparing root canals filled with EndoREZ sealer/EndoREZ® Points and EndoREZ sealer/conventional gutta-percha points.</p><p>Material and Methods: 84 human teeth were prepared and filled with gutta-percha points using the single cone technique. Roots were randomly divided into 6 groups: Apexit Plus, AH Plus, Copaifera sp oil, Polifil, EndoREZ, and EndoREZ/EndoREZ Points. After setting time, the roots were incorporated in a leakage model, which upper chamber contained a suspension of <em>Streptococcus mutans</em>, and lower chamber a broth. Leakage was assessed for turbidity in lower chamber for 60 days. Statistic analysis was performed using the nonparametric Kaplan-Meier method (p&lt;0.05).</p><p>Results: All experimental groups presented leakage during the study’s period. The medium time of leakage was: Apexit Plus and AH Plus 6,3 days, Polifil 5,1 days, Copaifera 1,2 days, and both EndoREZ groups infiltrated in the first day.</p><p>Conclusions: There was no statistically significant difference between the sealers Apexit Plus, AH Plus and Polifil, but they prevented leakage better than Copaifera sp oil and both EndoREZ groups. However, none of the tested sealers was capable of resisting coronal bacterial leakage for more than 22 days.</p>

2017 ◽  
Vol 22 (2) ◽  
Author(s):  
Camila Krusser Teixeira ◽  
Sabrina Sasso Da Silva ◽  
Silvana Beltrami Gonçalves Waltrick ◽  
Renata Dornelles Morgental ◽  
Fabiana Vieira Vier-Pelisser ◽  
...  

Objective: The aim of this study was to compare the performance of the new endodontic sealer GuttaFlow 2 with conventional sealers (AH Plus and EndoFill) in filling artificial lateral and secondary root canals, using two obturation techniques (cold lateral condensation and Tagger’s hybrid technique). Methods: Sixty single-rooted human teeth were selected and submitted to root canal preparation. Next, specimens were subjected to a tooth decalcification and clearing method. Artificial lateral and secondary canals were created at 2, 5, 9 and 12 mm from the root apex. The specimens were divided into 6 groups (n=10) according to the endodontic sealer (GuttaFlow 2, AH Plus or EndoFill) and the obturation technique (cold lateral condensation or Tagger’s hybrid technique). Filling of lateral and secondary canals was analyzed by stereomicroscopy. Images were obtained and transferred to the ImageJ software. Mean percentages of filling were calculated, considering total linear measurements of artificial canals and their filled portions. Data were compared by two-way ANOVA, followed by Bonferroni post-hoc test, at 5% significance level. Results: All materials showed the capacity of penetrating into artificial lateral and secondary canals. There was no significant difference (P>0.05) between sealers and obturation techniques. Only GuttaFlow 2 showed significant better flow into lateral canals compared to secondary canals (P


2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Jaya Siotia ◽  
Shashi Rashmi Acharya ◽  
Sunil Kumar Gupta

Objective. To compare the efficacy of ProTaper retreatment files in removing three different obturating materials.Study Design. Forty-five human, single-rooted premolars were divided into three experimental groups. Group 1 was obturated with gutta-percha and AH Plus sealer, Group 2 was obturated with gutta-percha and zinc oxide eugenol sealer, and Group 3 was obturated with GuttaFlow. Retreatment was done using the ProTaper universal rotary retreatment files. Root halves were visualized using magnifying loops at 3X magnification and optical stereomicroscope at 10X magnification. Images were analyzed using AutoCAD 2004 software to calculate area of the remaining debris in the canal. For statistical analysis were used variance test and ANOVA.Results. Total debris/canal area ratio between the three groups showed a statistically significant difference (P<0.001).Conclusion. ProTaper retreatment system did not produce completely clean canals in any of the groups. However, it had the best efficacy towards removing silicon based obturating material GuttaFlow.


2005 ◽  
Vol 13 (2) ◽  
pp. 176-179 ◽  
Author(s):  
Juliana Machado Barroso ◽  
Laise Daniela Carrasco ◽  
Alexandre Capelli ◽  
Danilo Mathias Zanello Guerisoli ◽  
Paulo César Saquy ◽  
...  

The aim of this study was to investigate, in vitro, the percentage of filling of simulated lateral canals in teeth obturated with TP medium and standardized gutta-percha points. Twenty human mandibular canines were prepared with LA Axxess (SybronEndo) and K³ Endo rotary system (SybronEndo) up to a #50 file, according to the Free Tip Preparation Technique. During instrumentation, the root canals were alternately irrigated with 1% sodium hypochlorite and 17% EDTA. Six artificial lateral canals were prepared at the apical third of each tooth. Then, the teeth were assigned to two groups (n=10): Group 1 - filled with TP medium master gutta-percha points (Dentsply, Mailleffer); Group 2 - filled with standardized master gutta-percha points (Dentsply, Mailleffer). Root canal filling was complemented with AH Plus sealer (Dentsply, Mailleffer) and accessory gutta-percha points (Dentsply, Mailleffer), according to the classic technique. The teeth were radiographed and the images obtained were digitized. Linear measurements of the percentage of filling of the artificial lateral canals in each group were accomplished on the Image Tool 2.02 software. Statistical analysis of the data using Mann-Whitney U non-parametric test evidenced significant difference (p<0.01) between the experimental groups. The group obturated with TP medium points yielded higher percentage of filling of the lateral canals. It may be concluded that the use of master gutta-percha points with larger taper resulted in better filling of the simulated lateral canals, as compared to the use of standardized master gutta-percha points.


2006 ◽  
Vol 14 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Márcia Carneiro Valera ◽  
Carlos Henrique Ribeiro Camargo ◽  
Alessandra Sverberi Carvalho ◽  
Eduardo Ramalho Pereira Gama

The objective of this study was to evaluate the apical leakage of retrograde cavities filled with Portland Cement (Concrebrás S/A-MG-Brazil), ProRoot MTA TM (Dentsply International, Johnson City, TN, USA) and Sealapex (Kerr Corporation, Orange, California, USA) with addition of zinc oxide (Odahcam Herpo Produtos Dentários Ltda, Rio de Janeiro, RJ, Brazil). Forty-two extracted single-rooted human teeth were decoronated and used for this study. The root canals were instrumented at 1.0mm short of the apical foramen using the step-back technique to an apical ISO size 60. The roots were obturated with gutta-percha points and sealer Sealapex (Kerr Corporation-USA) and then 3mm of each root apex was sectioned at a 90° angle. Ultrasonic retrograde preparation was performed with a diamond tip to 3mm depth and the roots were randomly divided into 3 groups according to the filling material: G1-Portland, G2-ProRoot MTA, G3- Sealapex zinc oxide-added cement. The root surfaces were covered with nail varnish up to 2mm from the apical foramen, immersed in simulated tissue fluid for 30 days, and then immersed in 0.2% Rhodamine B solution for 24 hours for evaluation of marginal leakage. The results showed mean leakage of 0.75, 0.35 and 0.35 for groups 1, 2 and 3, respectively; however, Kruskal-Wallis test revealed that there was no statistically significant difference among the results (p>0.05).


2004 ◽  
Vol 5 (2) ◽  
pp. 93-101 ◽  
Author(s):  
Oya Bala ◽  
L. Sibel Karadag ◽  
Emin Türköz ◽  
Tansev Mihçioğlu

Abstract In this study, the aim was to assess the in vitro apical microleakage of a resin-based sealer used with two different adhesives. Thirty nine freshly extracted maxillary incisors were used. The teeth were decoronated at the cemento-enamel junction with a water-cooled fissure bur. Chemo-mechanical debridement of the root canals was accomplished with the step-back technique. The smear layer was removed by 19% ethylenediamine tetra acetic acid (EDTA). The roots were then divided into three experimental groups of thirteen teeth in each. Specimens in group 1 were filled with gutta-percha, AH Plus sealer, and water-based adhesive system (Syntac Single Component). Group 2 specimens were filled with gutta-percha, AH Plus sealer, and acetone-based dentin adhesive (Prime & Bond NT). Specimens of group 3 were filled with only gutta-percha and AH Plus sealer (no adhesive was applied). The teeth were immersed into 2% methylene blue solution. Apical sealing qualities were assessed by measuring the linear dye penetration with a stereomicroscope. Dentin tubule penetration was observed under scanning electron microscopy (SEM). Results showed no statistically significant difference between the materials used, however, the leakage in group 2 was less than group 1 and 3. Citation Karadag LS, Bala O, Türköz E, Mihçioğlu T. The Effects of Water and Acetone-based Dentin Adhesives on Apical Microleakage. J Contemp Dent Pract 2004 May;(5)2:093-101.


2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Farzaneh Afkhami ◽  
Shifteh Nasri ◽  
Sara Valizadeh

Abstract Background Successful endodontic therapy requires prevention of bacterial leakage between the root canal filing and root-canal walls. Sealing quality of a root canal filling depends strongly on the sealing ability of the sealer used. The present study aimed to evaluate the bacterial leakage resistance of AH Plus sealer modified with silver nanoparticles. Methods Forty sound teeth were obturated using lateral compaction technique except for five teeth as the negative controls. After considering five teeth as the positive controls (filled without sealer), the remaining teeth were assigned to two groups (n = 15) in terms of the sealer used (AH Plus sealer and silver nanoparticle-modified AH Plus). Bacterial leakage was evaluated in saliva using the two-chamber technique in every 24 h. When all the samples in the test groups were contaminated, the study was terminated. The data were analyzed with log-rank statistical test. Results All samples in both experimental groups were contaminated during the 3-months period of observation. There was no significant difference in contamination time between study groups (P > 0.05). Conclusions Silver nanoparticles used in tested concentration did not improve the bacterial leakage resistance of AH Plus sealer.


2007 ◽  
Vol 54 (4) ◽  
pp. 224-230
Author(s):  
Dejan Markovic ◽  
Vesna Zivojinovic ◽  
Slavoljub Zivkovic

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.


2007 ◽  
Vol 54 (3) ◽  
pp. 184-190 ◽  
Author(s):  
Slavoljub Zivkovic ◽  
Borislava David-Burlica ◽  
Jelena Stanisic ◽  
Sonja Stojcic ◽  
Milica Medojevic-Jovanovic ◽  
...  

Aim: The purpose of this study was to determine the influence of the smear layer on the coronal microleakage in root canals obturated with laterally condensed cold gutta-percha or Thermafil and two root canal sealers. Materials and methods: Sixty extracted single-rooted human teeth with straight root canals were allocated to two experimental groups. The root canals were instrumented using the STEP-BACK technique, K reamers and K files and irrigated with 2.5 % NaOCl. The teeth in the first group were treated with 17% EDTA and 2.5 % NaOCl to remove the smear layer. The teeth in both groups were obturated either with laterally condensed cold guttapercha (28 teeth) or Thermafil gutta-percha with plastic cores (28 teeth) and RSA or AH-PLUS sealers. The specimens were evaluated for coronal microleakage using silver nitrate solution. The linear penetration of the dye was assessed using a binocular magnifying glass (x25), with an inserted micrometer scale. Results: The results showed low coronal microleakage in the group where the smear layer was removed in both the lateral compaction technique (RSA - 1.71mm and AHPLUS - 1.61mm) and the Thermafil groups - RSA (1.65mm) and AH-PLUS (1.66mm). In the group with the intact smear layer, greater microleakage was noticed in both groups, lateral compaction technique -RSA (3.06 mm) and AH-PLUS (2.83mm) and Thermafil technique -RSA (2.99mm) and AH-PLUS ( 2.83mm). The difference was statistically significant between the groups with and without the smear layer for both RSA (p< 0.001) and AH-PLUS sealers ( p<0.001). Conclusion: The present results suggest that the removal of the smear layer significantly improves the tightness of the coronal seal and reduces the coronal microleakage.


2006 ◽  
Vol 7 (4) ◽  
pp. 45-52 ◽  
Author(s):  
Emre Bodrumlu ◽  
Umut Tunga

Abstract Aim The aim of this study was to assess the apical sealing ability of Resilon™ obturation material. Methods and Materials Forty-two single rooted extracted human teeth were selected and randomly divided into three groups. They were obturated using lateral condensation with gutta-percha and AH 26/AH plus and Resilon™; also, 12 root canals were used as control groups. The apical leakage was measured using the dye penetration methodology. Results The teeth filled with gutta-percha and AH 26 displayed the most apical leakage. The least apical leakage was shown with Resilon™ Discussion The differences in leakage among the groups, gutta-percha with AH 26 (2.4 mm, SD:1.87), gutta-percha with AH plus (2.1 mm, SD: 1.69), and Resilon™ (1.7 mm, SD:1.32), were statistically significant (p< 0.001). Conclusion Based on the data of this study, all groups produced a satisfactory seal. However, Resilon™ and Epiphany™ sealer showed the least apical leakage. Citation Bodrumlu E, Tunga U. Apical Leakage of Resilon™ Obturation Material. J Contemp Dent Pract 2006 September;(7)4:045-052.


2019 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Aryadi Subrata ◽  
Anastasia Elsa Prahasti ◽  
Bernard Ongki Iskandar

Introduction: Root canal treatment is done to maintain the teeth to last as long as possible in the oral cavity. This can be done with three main stages, such as biomechanical preparation, sterilization and root canal filling. The hermetic seal of root canal filling has an important role in the success of root canal treatment. Objective: The aim of this in-vitro study is to compare the microbial leakage of root canals filled with RealSeal sealer/Resilon Points and AH Plus sealer/conventional gutta-percha points using single cone obturation technique and warm vertical compaction technique. Methods: Thirty-two extracted human mandibular premolars with single canals were decoronated to a standardized root length of 15 mm and prepared using crown-down technique to a master apical file size 30/.09. Teeth were divided into four experimental groups (n = 7 each group) and controls (n = 1 each group). Teeth in the first group were obturated using single master gutta percha cone and AH Plus sealer and second group were obturated with single Resilon gutta percha cone using RealSeal sealer. Third experimental group was filled with warm vertical condensed gutta-percha using AH Plus sealer and fourth group was filled with warm vertical condensed Resilon gutta-percha using RealSeal sealer. The coronal chamber of each sample were inoculated with Enterococcus faecalis. Results: There were no significant differences between the four experimental groups (P = 0.182). The differences occurred only in the survival time from each group. Group 4 (Warm Vertical Condensation - RealSeal) has the lowest leakage rate among other groups and the fastest leakage occurred in group 1 (Single Cone - AH Plus). Conclusion: The single-cone techniques does not insure durable apical seal against bacterial leakage. Warm vertical compaction technique using Resilon gutta-percha and RealSeal sealer appears to be more effective in minimizing bacterial leakage than gutta-percha and AH Plus sealer.


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