Differential of Antioxidant Ability, CD4+T Cells Count and Viral Load in HIV Infected Patients on cART in Yaounde, Cameroon

Background Decreased antioxidant ability is one of the worsening conditions in AIDS.We aimed to evaluate total antioxidant ability among others, and their variation in HIV infected patients following their CD4+T cells count and viral load, in a context of new ART scarcity in most LMICs. Material and Methods We conducted a cross sectional study on 167 individuals (76 controls, 33 treatments naïve and 58 HIV-1 infected patients on ART). We assessed their plasma total antioxidant ability (FRAP), malondialdehyde (MDA) and thiol (SH) groups using standard spectrophotometric methods, then we calculated lipid peroxidation index (LPI). Statistical analysis was performed using GraphPad Prism 6. Data were analyzed by two-tailed unpaired t-test for two groups’ comparison and ANOVA for more than two groups. Pearson correlation between CD4+T cells count, viral load and the above markers was determined; P ≤ 0.05 was considered statistically significant. Results The following controls/naïve/treated subjects’ values for FRAP(mM) (1.907±0.074/1.77±0.05/1.695±0.03); MDA(μΜ) (0.781±0.081/1.115±0.118/ 1.342±0.109); SH (μΜ) (2.747±0.130/1.582±0.197/1.498 ±0.140)and LPI (0.43±0.61/ 0.61±0.7/2.59±0.83) were all obtained with P ≤ 0.05. The FRAP increased only with 3TC+TDF+EFV and 3TC+ABC+NVP cART while MDA decrease significantly with the later(p=0.027). MDA and LPI significantly increased in heavily treated patients with p<0.0014 and p=0.0001 respectively. overall, the patients showed an increase of viral loads following a decrease of CD4+T cells (r= -0.803, p=0.016) but 3TC+TDF+EFV seem to better manage the both. The only significant correlation was established between SH groups and CD4+Tcells count (r=0.447; p=0.0006); Conclusion Our study showed that thiol groups may be protective againstCD4+Tcells count depletion and that the cART 3TC+TDF+EFV, 3TC+ABC+NVP may be helpful in fighting against free radical generation and particularly 3TC+TDF+EFV as controlling CD4+Tcells count and viral load in long term treated patients. The study particularly showed the implication of cART in increasing lipid peroxidation index following the treatment duration in heavily treated patients, which aggravated their conditions in an area where drug options are limited, calling for new drugs availability and personalized medicine.

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Functional Impairment of Central Memory CD4 T Cells Is a Potential Early Prognostic Marker for Changing Viral Load in SHIV-Infected Rhesus Macaques

PLoS ONE ◽

10.1371/journal.pone.0019607 ◽

2011 ◽

Vol 6 (5) ◽

pp. e19607 ◽

Cited By ~ 10

Author(s):

Hong He ◽

Pramod N. Nehete ◽

Bharti Nehete ◽

Eric Wieder ◽

Guojun Yang ◽

...

Keyword(s):

T Cells ◽

Viral Load ◽

Prognostic Marker ◽

Functional Impairment ◽

Cd4 T Cells ◽

Rhesus Macaques ◽

Central Memory ◽

Memory Cd4 T Cells

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EFFECT OF ADHERENCE TO ANTI-RETROVIRAL THERAPY ON CD4 T CELLS AND HIV VIRAL LOAD IN NEPALESE TERTIARY CARE HOSPITAL

World Journal of Pharmaceutical Research ◽

10.20959/wjpr20178-9095 ◽

2017 ◽

pp. 1793-1811

Author(s):

Pramila K. C.

Keyword(s):

T Cells ◽

Viral Load ◽

Cd4 T Cells ◽

Tertiary Care Hospital ◽

Tertiary Care ◽

Care Hospital ◽

Hiv Viral Load

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RNA sequencing of CD4 T-cells reveals the relationships between lncRNA-mRNA co-expression in elite controller vs. HIV-positive infected patients

PeerJ ◽

10.7717/peerj.8911 ◽

2020 ◽

Vol 8 ◽

pp. e8911

Author(s):

Chaoyu Chen ◽

Xiangyun Lu ◽

Nanping Wu

Keyword(s):

T Cells ◽

Rna Sequencing ◽

Highly Active Antiretroviral Therapy ◽

Cd4 T Cells ◽

Expression Profiles ◽

Pearson Correlation ◽

Elite Controller ◽

Hiv Positive ◽

Elite Controllers ◽

Functional Relationships

Background Elite controller refers to a patient with human immunodeficiency virus infection with an undetected viral load in the absence of highly active antiretroviral therapy. Studies on gene expression and regulation in these individuals are limited but significant, and have helped researchers and clinicians to understand the interrelationships between HIV and its host. Methods We collected CD4 T-cell samples from two elite controllers (ECs), two HIV-positive infected patients (HPs), and two healthy controls (HCs) to perform second-generation transcriptome sequencing. Using the Cufflinks software, we calculated the Fragments Per Kilobase of transcript per Million fragments mapped (FPKM) and identified differentially expressed (DE) mRNAs and long non-coding RNAs (lncRNAs), with corrected P value < 0.05 (based on a false discovery rate (FDR) < 0.05). We then constructed a protein-protein interaction network using cytoHubba and a long non-coding RNA-mRNA co-expression network based on the Pearson correlation coefficient. Results In total, 1109 linear correlations of DE lncRNAs targeting DE mRNAs were found and several interesting interactions were identified as being associated with viral infections and immune responses within the networks based on these correlations. Among these lncRNA-mRNA relationships, hub mRNAs including HDAC6, MAPK8, MAPK9, ATM and their corresponding annotated co-expressed lncRNAs presented strong correlations with the MAPK-NF-kappa B pathway, which plays a role in the reactivation and replication of the virus. Conclusions Using RNA-sequencing, we systematically analyzed the expression profiles of lncRNAs and mRNAs from CD4+ T cells from ECs, HPs, and HCs, and constructed a co-expression network based on the relationships among DE transcripts and database annotations. This was the first study to examine gene transcription in elite controllers and to study their functional relationships. Our results provide a reference for subsequent functional verification at the molecular or cellular level.

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283. HIV Protected Autologous Zinc Finger Nuclease Driven CCR5 Modified CD4 T-Cells CCR5 (SB-728-T) Reduce HIV Viral Load in CCR5 Δ32 Heterozygote Subjects During Treatment Interruption (TI): Correlates of Effect, and Effect of Cytoxan Pre-Conditioning Regimen

Molecular Therapy ◽

10.1016/s1525-0016(16)35296-0 ◽

2014 ◽

Vol 22 ◽

pp. S109 ◽

Cited By ~ 1

Keyword(s):

T Cells ◽

Viral Load ◽

Zinc Finger ◽

Cd4 T Cells ◽

Conditioning Regimen ◽

Treatment Interruption ◽

Zinc Finger Nuclease ◽

Hiv Viral Load

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Persistent viral load in patients on HAART is associated with a higher level of activation-induced apoptosis of CD4+ T cells

Journal of Antimicrobial Chemotherapy ◽

10.1093/jac/47.1.109 ◽

2001 ◽

Vol 47 (1) ◽

pp. 109-112 ◽

Cited By ~ 2

Author(s):

P.-M. Roger

Keyword(s):

T Cells ◽

Viral Load ◽

Cd4 T Cells ◽

Induced Apoptosis

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Fractional Dynamics of HIV with Source Term for the Supply of New CD4+ T-Cells Depending on the Viral Load via Caputo–Fabrizio Derivative

Molecules ◽

10.3390/molecules26061806 ◽

2021 ◽

Vol 26 (6) ◽

pp. 1806

Author(s):

Zahir Shah ◽

Rashid Jan ◽

Poom Kumam ◽

Wejdan Deebani ◽

Meshal Shutaywi

Keyword(s):

T Cells ◽

Viral Load ◽

Hiv Infection ◽

Cd4 T Cells ◽

Source Term ◽

Chaotic Behavior ◽

Numerical Study ◽

Public Health Problem ◽

Fractional Dynamics ◽

Global Public Health

Human immunodeficiency virus (HIV) is a life life-threatening and serious infection caused by a virus that attacks CD4+ T-cells, which fight against infections and make a person susceptible to other diseases. It is a global public health problem with no cure; therefore, it is highly important to study and understand the intricate phenomena of HIV. In this article, we focus on the numerical study of the path-tracking damped oscillatory behavior of a model for the HIV infection of CD4+ T-cells. We formulate fractional dynamics of HIV with a source term for the supply of new CD4+ T-cells depending on the viral load via the Caputo–Fabrizio derivative. In the formulation of fractional HIV dynamics, we replaced the constant source term for the supply of new CD4+ T-cells from the thymus with a variable source term depending on the concentration of the viral load, and introduced a term that describes the incidence of the HIV infection of CD4+ T-cells. We present a novel numerical scheme for fractional view analysis of the proposed model to highlight the solution pathway of HIV. We inspect the periodic and chaotic behavior of HIV for the given values of input factors using numerical simulations.

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Correlation between mandibular bone density, CD4 T-cells, and duration of HAART in HIV-infected children

Padjadjaran Journal of Dentistry ◽

10.24198/pjd.vol33no1.15894 ◽

2021 ◽

Vol 33 (1) ◽

pp. 12

Author(s):

Intan Maulani ◽

Risti Saptarini Primarti ◽

Irna Sufiawati ◽

Ratna Indriyanti ◽

Niekla Survia Andiesta

Keyword(s):

T Cells ◽

Bone Density ◽

Cd4 T Cells ◽

Pediatric Population ◽

Pearson Correlation ◽

Panoramic Radiograph ◽

Bone Cell ◽

Cross Sectional ◽

Mandibular Bone ◽

Perinatal Hiv

Introduction: Perinatal HIV infection has decreased adverse bone health effects and mineral accrual. HIV-infected patients have a multifactorial origin, including HIV bone cell infections, inflammatory cytokine effects on osteoblast and osteoclast activity, and HAART. The research objective was to examine the correlation between the mandibular bone density and CD4 T-cells with HAART duration in HIV-infected children. Methods: The mandibular bone density in the HIV-infected pediatric population was evaluated using a panoramic radiograph. The research design was a cross-sectional and univariate regression analysis for the sampling method. Mandibular density analysis using Spearman and Pearson correlation and HAART duration using Kendall correlation. Thirty-five HIV-infected children and seventeen non-HIV-infected children were recruited. Results: This study showed the correlation between HIV and non-HIV infected children (p<0.05). The correlation between CD4 T-cells and mandibular bone density was significant (p<0.05). Long term HAART and mandibular bone density have a significant correlation (p<0.001). This research showed correlations between mandibular bone density CD4 T-cells and duration of HAART in HIV-infected children. Conclusion: HIV-infected children require a regular mandibular cortical bone examination to detect the onset of osteopenia and osteoporosis to obtain appropriate therapy.

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Characteristics of Plasmacytoid Dendritic Cell and CD4+ T Cell in HIV Elite Controllers

Clinical and Developmental Immunology ◽

10.1155/2012/869505 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 3

Author(s):

Jean-Philippe Herbeuval ◽

Nikaïa Smith ◽

Jacques Thèze

Keyword(s):

T Cells ◽

Viral Load ◽

T Cell ◽

Cd4 T Cells ◽

Plasmacytoid Dendritic Cell ◽

Cd4 T Cell ◽

Cell Depletion ◽

T Cell Depletion ◽

Hiv Controllers ◽

Hiv 1

Despite variability, the majority of HIV-1-infected individuals progress to AIDS characterized by high viral load and massive CD4+ T-cell depletion. However, there is a subset of HIV-1-positive individuals that does not progress and spontaneously maintains an undetectable viral load. This infrequent patient population is defined as HIV-1 controllers (HIV controllers), and represents less than 1% of HIV-1-infected patients. HIV-1-specific CD4+ T cells and the pool of central memory CD4+ T cells are also preserved despite immune activation due to HIV-1 infection. The majority of HIV controllers are also defined by the absence of massive CD4+ T-cell depletion, even after 10 years of infection. However, the mechanisms involved in protection against HIV-1 disease progression have not been elucidated yet. Controllers represent a heterogeneous population; we describe in this paper some common characteristics concerning innate immune response and CD4+ T cells of HIV controllers.

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