scholarly journals Effects of Prosopis africana (Guill. & Perr.) Taub. and Ficus mucoso Ficalho ethanolic leaves extract in the control of Callosobruchus maculatus (Fabricius, 1775) in stored cowpea

2021 ◽  
Vol 117 (3) ◽  
pp. 1
Author(s):  
Tosin Damilola OJUYEMI ◽  
Robert Omotayo UDDIN II ◽  
Gbonjubola Victoria AWOLOLA ◽  
Suleiman MUSTAPHA ◽  
AbdRahaman Adebowale LAWAL
Keyword(s):  
Mortality Rate ◽  
Callosobruchus Maculatus ◽  
Smallholder Farmers ◽  
Seed Viability ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Phytochemical Analysis ◽  
Sustainable Pest Management ◽  
Prosopis Africana ◽  
Negative Effect

<p>The study investigated the effectiveness of <em>Prosopis africana</em> and <em>Ficus mucoso</em> ethanolic leave extract in the control of <em>Callosobruchus maculatus</em> infesting cowpea. Treatments were applied at different concentrations (10 %, 30 % , 50 %, and 0 %) on cowpea. Five pairs of newly emerged adult <em>C. maculatus</em> were introduced into each treatment. The two botanicals were evaluated on the insecticidal effects it has on the insect and data were recorded on adult mortality, oviposition rate, larvae, pupae, and adult emergence, seed viability, and phytochemicals present in both botanicals. Results revealed that both treatments had insecticidal potentials, adversely reducing the number of eggs, larvae, and pupae of <em>C. maculatus</em> with <em>P. africana</em> having the highest mean mortality rate at 50 % concentration. Observations further indicated that the botanicals had no negative effect on seed viability. The phytochemical analysis revealed the presence of some bioactive compounds such as terpenoids, flavonoids, alkaloids, saponin, steroids, and tannin, <em>P. africana</em> mostly rich in them than <em>F. mucoso</em>. Though both extracts were effective, <em>P. africana</em> performed better in the control of the bruchid beetle indicating plausible usefulness in sustainable pest management by smallholder farmers and consumers of cowpea in environments where the plants are in abundance.</p>

scholarly journals Perforation index assessment of cowpea seeds against cowpea bruchid, Callosobruchus maculatus (Fabricius) [Coleoptera: Chrysomelidae], infestation using Piper guineense

2020 ◽  
Vol 81 (1) ◽  
Author(s):  
Kayode David Ileke ◽  
Jacobs Mobolade Adesina ◽  
Luke Chinaru Nwosu ◽  
Abimbola Olagunju
Keyword(s):  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Contact Toxicity ◽  
Toxicity Assay ◽  
Piper Guineense ◽  
Cowpea Seeds ◽  
Leaf Powder ◽  
Human Use ◽  
Seed Extracts

Abstract Background Powders and extracts of Piper guineense seeds and leaves were assessed for insecticidal activities against Callosobruchus maculatus in the laboratory at temperature and relative humidity of 29.6 °C and 75.9%, respectively. Bioactive compounds in P. guineense leaves and seeds were also investigated. The powders were tested at rates 1.0, 2.0 and 4.0 g/20 g cowpea seeds while extracts were tested at 1.0, 2.0 and 3.0%. Results Results of contact toxicity assay of the seed powder caused 100% adult mortality at 96 h post-treatment period whereas leaf powder evoked 90% adult mortality within the same period at concentration of 1.0 g/20 g cowpea seeds. Low adult emergence was observed on cowpea seeds treated with 1 g of seed powder with percentage adult emergence of 10.0% and inhibition rate (IR) of 97.5%. Beetle Perforation Index (BPI) obtained from treated cowpea seeds was significantly different (P < 0.05) from BPI of untreated seeds. Extracts of P. guineense seed were more toxic than seed powder. Piper guineense seed extract caused 87.5% adult mortality of C. maculatus while leaf extract caused 70.0% adult mortality within 24 h of infestation at concentration of 1%. Progeny development of C. maculatus was completely inhibited in cowpea treated with 2% and 3% leaf and seed extracts of P. guineense. β-Pinene was the most abundant active compound in P. guineense seed (55.6%) and leaf (48.4%). β-Phellandrene occurred 38.2% in seeds while Ocimene had the least value of 0.2% in seed and 0.5% in leaf. Conclusion The study showed that P. guineense seed powder and extracts were more effective than leaf powder and extract. Utilization of plant products as alternative to synthetic insecticides in protecting cowpea seeds against C. maculatus should be encouraged for enhanced food safety and security. Piper guineense is used as spice and medicine and interestingly safe for human use.

scholarly journals Potentials of Cow Dung Ash as Protectants of Cowpea Seeds against Callosobruchus maculatus F. (Coleoptera: Chrysomelidae)

2020 ◽  
Vol 2 (1) ◽  
pp. p128
Author(s):  
Mohammed Suleiman ◽  
Fatima M. Haruna
Keyword(s):  
Callosobruchus Maculatus ◽  
Lethal Dose ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Cow Dung ◽  
Weight Losses ◽  
Cowpea Seeds ◽  
Randomized Design ◽  
Petri Dishes ◽  
Different Doses

Experiments were conducted to assess the protectant ability of cow dung ash (CDA) to cowpea seeds against Callosobruchus maculatus F. under laboratory conditions of 50-65% R.H and 28-32oC. The CDA at different rates of 0.625, 1.25, 2.50, 5.00, 10.00% (w/w) incorporated with cypermethrin powder at 0.625% were applied to 20 g of cowpea seeds in petri dishes. None of the ashes or cypermethrin was added to the control. Ten beetles were introduced into each of the petri dishes and covered with muslin cloth. The experiment was arranged in a completely randomized design (CRD) and replicated three times. Effects of the treatments on adult mortalities as well as median lethal dose (LD50) of CDA against C. maculatus were determined. Adult emergence and weight losses in seeds treated with CDA at different doses were assessed. Findings of the study revealed that the adult mortality of C. maculatus among different CDA treatments differed significantly (p < 0.05) and ranged from 13.33 to 100.00% 72 hours after treatment (HAT). Results of the study also showed that LD50 of CDA against C. maculatus at 24 hours after treatment (HAT) was 5.51 % (w/w). No adults emerged from the treated seeds and weight losses were observed in those treated with the lowest dose and the control only. Therefore, CDA could be utilized to protect cowpea seeds from C. maculatus infestations during storage.

scholarly journals Assessment of Insecticidal Properties of Products From Seeds of Carica papaya Against Callosobruchus Maculatus (Fabricius)

2018 ◽  
Vol 5 (4) ◽  
pp. 142
Author(s):  
Joy Ejemen Idoko ◽  
Bukola Mary Atere ◽  
Raphael Abiodun Adebayo
Keyword(s):  
Pest Management ◽  
Carica Papaya ◽  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Post Treatment ◽  
Application Rates ◽  
Cowpea Seeds ◽  
Randomized Design ◽  
Papaya Seed

Powder and ash of Carica papaya seed extracted from riped and unriped C. papaya fruits obtained from Teaching and Research Farm of the Federal University of Technology, Akure were evaluated for their insecticidal potential against Callosobruchus maculatus (Fabricius) at application rates of 0.0, 0.2, 0.4, 0.6, 0.8 and 1.0g per 20g of cowpea seeds. The experiment was carried out at the pest management laboratory of the Department of Crop, Soil and Pest Management under ambient laboratory conditions of 27±20C temperature and 70±5% relative humidity. Each treatment was infested with 5 pairs of C. maculatus. The experiment was laid out in Completely Randomized Design with each treatment replicated three times. Mortality of cowpea beetles were assessed at 24, 48 and 72hours post treatment. Thereafter data were collected on oviposition, adult emergence, weevil perforation index and seed germination. The results obtained indicated that adult mortality of C. maculatus increased with increasing application rates of powders and ashes of ripe and unripe C. papaya seeds. 100% mortality was observed on cowpea seeds treated with powders and ashes of unripe C. papaya seed at 0.8 and 1.0g application rates at 72hours post treatment. Powders and ashes of unripe C. papaya seed significantly reduced the oviposition potential of adult C. maculatus and caused significant reduction of damaged seeds. Highest number of seeds that germinated was recorded on the seeds treated with powders and ashes of unripe C. papaya seed at 0.8 and 1.0g application rates.

scholarly journals Effects of interaction between cowpea variety and the application of plant powders on Callosobruchus maculatus (Fabricius) (Coleoptera: Chrysomelidae) infestation of stored cowpea seeds

2020 ◽  
pp. 33-44
Author(s):  
Owolabi Moshood Azeez ◽  
Adebola Adedoyin Osipitan ◽  
Isaac Oludayo Daniel ◽  
Olufemi Olutoyin Richard Pitan
Keyword(s):  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Management Scheme ◽  
Cowpea Seeds ◽  
Randomized Design ◽  
Hyptis Suaveolens ◽  
Plant Powders ◽  
Seed Resistance ◽  
Cowpea Variety

An integrated pest management scheme involving seed resistance and the application of powder of Cymbopogon citratus, Alstonia boonei, Hyptis suaveolens, Azadirachta indica, Loranthus braunii and Lycopersicum esculentum as protectant against the seed bettle, Callosobruchus maculatus (Fabricius), was evaluated in the laboratory at ambient temperature (29±3°C) and relative humidity (65±5%). Six resistant cowpea accessions selected from previous studies were treated with the six plant powders at 1.25% w/w in a 6×7 factorial arrangement fitted into a completely randomized design with five replicates. Seed treatment with any of the six plant powders on six accessions resulted in significantly higher adult mortality, lower oviposition rate, adult emergence inhibition, lower seed damage and higher seed germination over the control, Protection ability of the plant powders was influenced by the cowpea accessions used.

scholarly journals Efficacy of Piper guineense Schumach. seed powder in the control of Callosobruchus maculatus (Fabricius, 1775) (Coleoptera: Chrysomelidae: Bruchinae) and on the nutritional and organoleptic characteristics of stored cowpea Vigna unguiculata (L.) Walp.

2018 ◽  
Vol 87 (2) ◽  
pp. 119-140
Author(s):  
Frank O. Ojiako ◽  
Adeyinka A. Adesiyun ◽  
Gabriel O. Ihejirika ◽  
Sunday A. Dialoke
Keyword(s):  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Adult Mortality ◽  
Proximate Analysis ◽  
Crude Fibre ◽  
Piper Guineense ◽  
Seed Damage ◽  
Cowpea Seeds ◽  
Organoleptic Characteristics ◽  
Cowpea Bruchid

Abstract Brown pepper (Piper guineense) seed powder, used for culinary and medicinal purposes, was evaluated in the laboratory with a conventional storage chemical, actellic 2% dust (Pirimiphos-methyl), as standard, to protect stored cowpea against the cowpea bruchid, Callosobruchus maculatus. Four rates of the seed powder were used (2.5, 5.0 and 10.0 g/100 g seed with a control, 0.0 g) while actellic dust was applied at 1.0, 2.0, 3.0 g/100 g seed and 0.0 g/100 g seed as control. Data on percentage mortality, oviposition, adult emergence and seed damage were collected over a six-month period. Weevil perforation indexes (WPI) were estimated with the seed damage data. All data were analysed for variance at P≤0.05. Actellic dust at the lowest dose inflicted adult mortality (90 – 100%) in the first 48 h after treatment and prevented oviposition (95 – 100%) in the first five months of storage compared to the control. The highest rate of Piper guineense application caused mortality of up to 90.0% in 48 h, reduced oviposition (70 – 80%), decreased adult emergence (15.90 to 28.40 insects) as against the control (104.7 – 272.7 insects) and reduced seed damage by 80 % with a WPI of between 33.3 and 10.0 %. Proximate analysis and germination tests indicated that the treatment materials increased the moisture and crude fibre content, decreased crude protein content but had no significant effect on the viability of stored seeds. Organoleptic tests demonstrated that treatment materials had no significant negative effect on taste, odour, texture, appearance and overall acceptability of cooked cowpea seeds after six months of storage. It can be safely concluded that the powdered form of P. guineense seed at the highest doses (5.0 and 10.0 g) gave significant protection of cowpea seeds against the cowpea bruchid, which compared favourably with actellic dust when applied topically.

scholarly journals Assessment of bone charcoal dusts of six species of mammal for the postharvest control of Callosobruchus maculatus Fabricius (Coleoptera: Chrysomelidae) infestations of cowpea seeds: can the charcoal dust affect seed viability in laboratory and in field?

2019 ◽  
Vol 3 (4) ◽  
pp. 279-287 ◽  
Author(s):  
Luke Chinaru Nwosu
Keyword(s):  
Callosobruchus Maculatus ◽  
Bos Taurus ◽  
Insect Pest ◽  
Larval Density ◽  
Seed Viability ◽  
Control Process ◽  
Adult Emergence ◽  
Ovis Aries ◽  
Cowpea Seeds ◽  
Bone Charcoal

Abstract Objectives The possibility that bone charcoal dusts of some species of mammal will control the major insect pest of stored cowpea seeds and the implications on seed viability (after pest control process) were investigated in the laboratory at ambient temperature (30 ± 3°C) and relative humidity (70 ± 5%) and in field. Materials and Methods Standard entomological and agronomical techniques were used. Results The results showed that at 1.0% w/w dosage, the bone charcoal dusts of Ovis aries and Bos taurus were more effective than permethrin standard insecticide in killing adult Callosobruchus maculatus Fab. infesting cowpea seeds in storage. Bos taurus had a faster action speed than permethrin at 1.0% w/w. At 1.5% w/w of dust, all the species of mammal investigated deterred oviposition considerably in C. maculatus females, whereas only the bone charcoal dusts of O. aries and Sus scrofa were comparable with permethrin in suppressing adult emergence and seed damage; S. scrofa only was comparable with permethrin in reducing larval density. Bone charcoal dusts of O. aries and S. scrofa require chemical and olfactory analyses to know if they might have caused irritation to the insects and disrupted insect circadian rhythm, affecting behaviour and mating activities, adversely. The bone charcoal dusts of the six species of mammal at a highest test dose of 1.5% w/w allowed high seed viability in both laboratory and field. Conclusion The study recommends the use of bone charcoal dusts of O. aries and S. scrofa at economical- and quality-favourable dose 1.5% w/w for managers seeking to control C. maculatus insect attacking cowpea seeds in storage. The transitive components of the bones, abrasion of the insect epicuticle lipid layer by the charcoal dust, and combustion-related toxic factors were responsible for the insecticidal activities of the bone charcoal dusts of the mammals.

scholarly journals Comparative response of Callosobruchus maculatus (Fab.) (Coleoptera: Chysomelidae) to Momordica charantia and Acalypha wilkesiana powders in stored cowpea (Vigna unguiculata) (Walp.)

2020 ◽  
Vol 14 (2) ◽  
pp. 224-230
Author(s):  
Oyewale Rufus Adeboye ◽  
Mercy Olayinka Oni ◽  
Jacobs Mobolade Adesina
Keyword(s):  
Weight Loss ◽  
Callosobruchus Maculatus ◽  
Adult Emergence ◽  
Dosage Level ◽  
Adult Mortality ◽  
Momordica Charantia ◽  
Small Scale ◽  
Cowpea Seeds ◽  
Plant Powders ◽  
Acalypha Wilkesiana

Post-harvest preservation of cowpea in developing countries has been a major challenge owing to Callosobruchus maculatus infestation. The study compares response of C. maculatus (F) to Momordica charantia and Acalypha wilkesiana powders in stored cowpea seeds under laboratory atmosphere. Five pairs of freshly emerged C. maculatus adults were introduced into Petri-dish containing 20 g of cowpea admixed with 0.2, 0.4, 0.6 and 0.8 g of each plant powder, replicated thrice and arranged in a complete randomization design. Adult mortality was recorded at 24 h interval for 5 days, oviposition was recorded after 48 h. While newly emerged insects were recorded 30-35 days after infestation and weight loss was also determined. The treatments showed significant increase in C. maculatus adult mortality as the time of exposure and dosage increases, with highest values (90 and 100% for A. wilkesiana and M. charantia, respectively) at 120 h of exposure and 0.8 g dosage level. At 0.8 g, A. wilkesiana (66.3) and M. charantia (63.0) treated seeds recorded significantly lower number of eggs laid. Similar trend was equally observed for adult emergence and percentage weight loss. The study revealed that both plants had significant insecticidal effects and clearly showed that M. charantia was more effective than A. wilkesiana, but did not show higher significant differences in all the parameters assessed. This indicated that these plant powders have prospective to safeguard cowpea seeds in store from C. maculatus infestation and damage. Therefore, these plant should be included in the Integrated Pest Management Strategies for cowpea storage by small scale farmers.

scholarly journals Isolation and Characterization of Eugenia Aromatica Oil Extract Against Tropical Warehouse Moth Ephestia cautella [Lepidoptera: Pyralidae] In Cocoa Beans

2019 ◽  
Vol 4 (2) ◽  
Keyword(s):  
Adult Emergence ◽  
Adult Mortality ◽  
Raw Material ◽  
Ephestia Cautella ◽  
Cocoa Bean ◽  
Phytochemical Analysis ◽  
Cocoa Beans ◽  
Egg Hatchability ◽  
Isolation And Characterization ◽  
Oil Extract

Cocoa bean is a raw material used for the production of chocolate and other confectionaries. Ephestia cautella is the major pest of dried cocoa beans in storage and synthetic insecticide like organochlorides and organophosphates are the major insecticides used to control this pest in storage which further post health hazard to man and his environment. This then necessitate the search for insecticide of plant origins which are bio-degradable and non-toxic to man. This study investigates the contact and fumigant efficacy of the powder and oil extract of Eugenia aromatica on the developmental stages of E. cautella. Powders of E. aromatica were administered at different concentrations (0.5g, 1.0g, 1.5g, 2.0g, and 2.5g). The oil from E.aromatica was extracted with ethanol using soxhlet extractor and redistilled using rotary evaporator and tested as fumigant insecticidal against development stages of E. cautellaat 0.5ml, 1.0ml, 1.5ml, 2.0ml, and 2.5ml. Egg hatchability, adult emergence, larvae and adult mortality of E.cautella were used as indices of insecticidal activities at 24hrs, 48hrs, 72hrs, and 96hrs post-treatment. Essential oil obtained from the plant was purified using thin layer chromatography and analysed by Gas Chromatography -Mass Spectrometer (GC-MS). Result obtained shown that E. aromatica powder and oil completely inhibited egg hatchability and adult emergence both as contact and fumigant. Except the 0.5g of E. aromatica powder that recorded 50.00% larva mortality and 51.67% adult mortality, other treatment concentrations recorded 90-100% larva and adult mortality. At 2.5ml oil extract tested as contact and fumigant larvicides after 96hrs recorded 92.98% and 98.23% mortality respectively. Results from phytochemical analysis of the oil showed that the major components were eugenol (82.044%) and Caryophyllene (11.716%). These findings suggested that E aromatica extract could be a potential source of insecticide which may be used for the production of biopesticide.

scholarly journals Effect of Tasmanian blue gum (Eucalyptus globulus Labill.) leaf extract on cowpea weevil (Callosobruchus maculatus [Fabricius, 1775], Coleoptera: Chrysomelidae)

2020 ◽  
Vol 116 (2) ◽  
pp. 351
Author(s):  
Samuel Femi BABATUNDE ◽  
Abdulrasak Kannike MUSA
Keyword(s):  
Mortality Rate ◽  
Eucalyptus Globulus ◽  
Untreated Control ◽  
Callosobruchus Maculatus ◽  
Adult Mortality ◽  
Cowpea Weevil ◽  
Grain Damage ◽  
Leaf Powder ◽  
Cowpea Seed ◽  
Blue Gum

<div class="WordSection1"><p>A laboratory study was carried out to examine the efficacy of solvent extract of <em>Eucalyptus globulus</em> leaves in reducing post-harvest losses caused by cowpea weevil<em> </em>(<em>Callosobruchus maculatus</em>) on cowpea seed variety RSH 256. 450 g of <em>E. globulus </em>leaf powder were put in 1000 ml flask and 450 ml of n-hexane was used as the solvent. The extract was tested at dosages of 50, 100 and 150 µl 50 g<sup>-1</sup> of cowpea grains on the weevil in stored cowpea. The different rates of treatment recorded significant differences (<em>p</em> &lt; 0.05) in causing adult mortality compared to the untreated control. The different rates of treatment also recorded significant differences (<em>p</em> &lt; 0.05) in emergence of F1 adults of each treatment compared to the control. The percentage masst loss and grain damage were also suppressed as a result of treatment with the plant material compared to the untreated control. However, among the treatments 150 µl 150 g<sup>-1</sup> cowpea recorded the highest adult mortality rate and lowest emergence while control had the lowest mortality rate and the highest emergence of the insect. The rates of application were indicative of bioactive characteristics of the plant extract.<strong></strong></p></div>

scholarly journals INSECTICIDAL ACTIVITY OF PIPER GUINEENSE (SCHUMACH AND THONN) SEED OIL AGAINST CALLOSOBRUCHUS MACULATUS (F.) (COLEOPTERA: CHRYSOMELIDAE) IN STORED COWPEA SEEDS

2020 ◽  
Vol 8 (8) ◽  
pp. 262-270
Author(s):  
Chukwunonso O. Nzelu ◽  
Kingsley C. Emeasor ◽  
Nnaemeka J. Okonkwo
Keyword(s):  
Seed Oil ◽  
Callosobruchus Maculatus ◽  
Insect Pest ◽  
Effective Means ◽  
Seed Viability ◽  
Adult Mortality ◽  
Economic Losses ◽  
Piper Guineense ◽  
Cowpea Seeds ◽  
Cowpea Seed

Post-harvest losses due to insect pest infestation in storage constitute a challenge to sustainable food security and economic losses to farmers throughout the world. Use of synthetic chemicals have been the most effective means of reducing losses in storage. Unfortunately, these toxicants have adverse effects in humans and the environment. There is renewed search for safer plant products as alternatives. In this study, we assessed the potential use of Piper guineense seed oil in the control of cowpea bruchid, Callosobruchus maculatus (F.) infestation on stored cowpea seeds. Adult mortality, oviposition rate, F1 progeny emergence, cowpea seed damage and seed germinability were the indices considered in the assessment under laboratory conditions. The setup of the experiment was completely randomized design (CRD). Statistical analysis of the data was by analysis of variance (ANOVA). Adult C. maculatus mortality was dose-dependent with higher dosages inducing 100% mortality. The number of eggs laid on the treated seeds were significantly (P<0.001) reduced. Progeny emergence and feeding damage were completely inhibited at higher dosages. The seed oil has no adverse effect on cowpea seed viability. The results indicated that P. guineense seed oil has protectant potential against insect pest damage in storage and a good alternative to synthetic insecticides in the control of insect pest of stored products.

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