scholarly journals Dynamics of TNF-α and IFN-γ in adult patients with measles depending on the development of complications

2021 ◽  
Vol 23 (6) ◽  
pp. 834-838
Author(s):  
S. O. Bilokobyla ◽  
O. V. Riabokon ◽  
Yu. Yu. Riabokon ◽  
N. V. Onishchenko
Keyword(s):  
Elevated Serum ◽  
Serum Levels ◽  
High Serum ◽  
Adult Patients ◽  
Medical Laboratory ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Acute Period ◽  
Tnf Α ◽  
Ifn Γ

The aim is to define the dynamics of TNF-α and IFN-γ serum levels in adult patients with measles depending on the development of complications. Materials and methods. An examination of 175 adult patients with measles revealed a range of complications and dynamics of serum TNF-α and IFN-γ depending on the development of complications. The serum contents of TNF-α (Invitrogen, Austria) and IFN-γ (Invitrogen, Austria) in patients and 30 healthy individuals were determined by enzyme-linked immunosorbent assay on the basis of the Training Medical Laboratory Center of Zaporizhzhia State MedicalUniversity. Results. It was shown that the vast majority of hospitalized adult patients with measles developed complications (70.6 %) and these latter were predominantly respiratory (61.7 %) and gastrointestinal (44.0 %). In the period of measles rash, on day 4.8 ± 0.2 in the presence of complications, the serum TNF-α content was 11 times higher than that in healthy individuals (P < 0.05) and in patients with uncomplicated measles (P < 0.05), and did not differ from healthy individuals (P > 0.05) in the absence of complications. The development of measles complications in adult patients was associated with higher serum levels of TNF-α both at hospitalization (P = 0.003) and after 10 days of the disease (P = 0.005). The serum content of IFN-γ in adult measles patients with rash at hospitalization on day 4.8 ± 0.2 exceeded that in healthy indivi­duals both in the presence of complications (P < 0.05) and in uncomplicated measles (P < 0.05). The serum content of IFN-γ was 19.3 times (P < 0.05) higher in patients with complicated measles than that in patients without complications. High serum levels of IFN-γ were associated with the development of complications during acute period of measles (P = 0.0001). Conclusions. Complications occurred in most hospitalized adult patients with measles (70.6 %), that was accompanied by more pronounced immunological changes. The development of measles complications in adult patients was associated with higher serum levels of TNF-α both at hospital admission on day 4.8 ± 0.2 (gamma 0.77; P = 0.003) and in the dynamics after 10 days of the disease (gamma 0.56; P = 0.005). Elevated serum levels of IFN-γ were associated with the development of complications during the acute period of measles (gamma ratio 0.46; P = 0.0001).

scholarly journals Curcumin Ameliorate Diabetes type 1 Complications through Decreasing Pro-inflammatory Cytokines in C57BL/6 Mice

2020 ◽  
Author(s):  
Yaser Jafari Khataylou ◽  
Somayeh Ahmadiafshar ◽  
Reza Rezaei ◽  
Saeid Parsamanesh ◽  
Golbahar Hosseini
Keyword(s):  
Serum Levels ◽  
Treated Group ◽  
Diabetic Group ◽  
Enzyme Linked Immunosorbent Assay ◽  
Diabetic Mice ◽  
Citrate Buffer ◽  
C Peptide ◽  
Tnf Α ◽  
Ifn Γ

Type 1 diabetes is a chronic autoimmune disease of beta cells in the islets of Langerhans, which are responsible for making insulin. Even with insulin therapy, inflammatory complications will develop in the long term. The present study examines changes in serum levels of interleukin (IL)-6, IL-17, IL-10, tumor necrosis factor (TNF)-α, interferon (IFN)-γ, C-peptide, Insulin as well as fasting blood sugar (FBS) in control, diabetic and diabetic treated with curcumin groups. Thirty inbred C57BL /6 mice were randomly divided into three groups of 10 mice: group A consisted of healthy mice receiving citrate buffer, group B included a group of diabetic mice, and group C was a group of diabetic mice treated with curcumin. The cytokine levels were measured in the supernatant of stimulated splenocytes using enzyme -linked immunosorbent assay (ELISA). Radioimmunoassay was used to measure insulin and c-peptide levels. The FBS was measured by an automatic glucometer device. The levels of IL-6, IL-17, and IFN-γ, as well as FBS, was significantly decreased in the treated group with curcumin compared to the diabetic group mice (p<0.05). TNF-α levels were also low, but the difference was not significant. IL-10, plasma C-peptide, and insulin significantly increased in the supernatant of stimulated splenocytes of treated diabetic group than in the diabetic group (p<0/05). According to the results, this study supports the anti-diabetic and anti-inflammatory effects of curcumin; however, more studies are needed to investigate theeffects of curcumin and the dose-response relationship in this disease.  

scholarly journals TGF-Β as a Marker of Ulcerative Colitis and Disease Severity

2018 ◽  
Vol 19 (3) ◽  
pp. 229-236 ◽  
Author(s):  
Marina Jovanovic ◽  
Natasa Zdravkovic ◽  
Ivan Jovanovic ◽  
Gordana Radosavljevic ◽  
Nevena Gajovic ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Previous Treatment ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Specific Marker ◽  
Compensatory Mechanism ◽  
Healthy Individuals ◽  
Highly Sensitive ◽  
Inflammatory Processes ◽  
Ifn Γ

Abstract Ulcerative colitis (UC) represents chronic inflammation of the large intestine. Immune response plays an important role in disease genesis and progression. Activated leukocytes secrete several cytokines that actively regulate the inflammatory response in UC. The aim of this study was to determine levels of cytokines IL-17, IL-27, IFN-γ and TGF-β in patients with UC and to test them as biomarkers for disease. The blood samples of 24 patients with ulcerative colitis without previous treatment and 37 healthy individuals were analyzed. Serum levels of IL-17, IL-27, IFN-γ and TGF-β were measured using sensitive enzyme-linked immunosorbent assay (ELISA) kits. Serum levels of IL-17, IL-27, IFN-γ and TGF-β were increased in patients with UC, compared to healthy controls (p=0.022; p=0.001; p=0.001; and p=0.002; respectively). Ratios of cytokines IL-27/IL-17, IFN-γ/TGF-β and IL-17/TGF-β were significantly higher in group of patients with UC (p=0.002; p=0.002; p=0.003; respectively). Serum value of TGF-β higher than 20 pg/ml presents a highly sensitive and specific marker for UC. We believe that increased production and predominance of immunosupressive TGF-β may represent compensatory mechanism for ongoing pro-inflammatory processes in UC.

Genetic variants of interferon-gamma and its mRNA expression and inflammatory parameters in the pathogenesis of vitiligo

2017 ◽  
Vol 95 (4) ◽  
pp. 474-481 ◽  
Author(s):  
Rehab A. Karam ◽  
Haidy E. Zidan ◽  
Mohamed H. Khater
Keyword(s):  
Elevated Serum ◽  
Serum Levels ◽  
Intercellular Adhesion Molecule ◽  
Control Group ◽  
Intercellular Adhesion Molecule 1 ◽  
Inflammatory Parameters ◽  
Increased Risk ◽  
Tnf Α ◽  
Ifn Γ

Although genetics plays an essential role in the pathogenesis of vitiligo, vitiligo pathogenesis is still unclear. Our aim was to investigate the role of IFN-γ expression and polymorphism in vitiligo susceptibility and whether intercellular adhesion molecule-1 (ICAM-1), tumor necrosis factor (TNF)-α, and TNF-β play a role in vitiligo pathogenesis as important inflammatory parameters. Eighty-five patients with vitiligo and 90 controls were investigated for IFN-γ gene expression by quantitative real-time PCR and genotyped for IFN-γ +874T/A (rs2430561) and IFN-γ +2109A/G (rs1861494) gene polymorphisms by sequence-specific primer (SSP)-PCR and PCR-restriction fragment length polymorphism (RFLP), respectively. Serum levels of inflammatory parameters were measured using ELISA. Frequencies of the +874 TT genotype and T allele were significantly higher in patients with active vitiligo than in stable patients (P = 0.01 and 0.03, respectively). Calculation of odds ratio suggested a 1.7-fold increased risk of vitiligo in individuals having the TA haplotype. We observed overexpression of IFN-γ mRNA with elevated serum levels of IFN-γ, ICAM-1, TNF-α, and TNF-β in patients with vitiligo when compared with the control group (P = 0.001, for all). In addition, these levels were elevated in patients with active vitiligo compared with stable patients with vitiligo (P = 0.008, 0.006, 0.01, 0.01, and 0.03, respectively), which suggests the involvement of these cytokines in disease activity. In conclusion, IFN-γ is a promising immunological marker in vitiligo pathogenesis.

High Serum Levels of CXCL11 in Mixed Cryoglobulinemia Are Associated with Increased Circulating Levels of Interferon-γ

2011 ◽  
Vol 38 (9) ◽  
pp. 1947-1952 ◽  
Author(s):  
ALESSANDRO ANTONELLI ◽  
CLODOVEO FERRI ◽  
SILVIA MARTINA FERRARI ◽  
ILARIA RUFFILLI ◽  
MICHELE COLACI ◽  
...  
Keyword(s):  
Strong Relationship ◽  
Serum Levels ◽  
Mixed Cryoglobulinemia ◽  
Interferon Γ ◽  
High Serum ◽  
Hepatitis C Infection ◽  
T Helper 1 ◽  
Tnf Α ◽  
Ifn Γ ◽  
Factor Α

Objective.No study has evaluated circulating chemokine C-X-C motif ligand (CXCL)11 in patients with “mixed cryoglobulinemia and chronic hepatitis C infection” (MC+HCV). We measured CXCL11, and correlated this measurement to the clinical phenotype.Methods.Serum CXCL11, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) were assayed in 97 MC+HCV patients and in 97 sex- and age-matched controls.Results.MC+HCV patients showed significantly higher mean CXCL11 serum levels than controls (254 ± 295, 68 ± 16 pg/ml, respectively; p = 0.0002; ANOVA). CXCL11 was significantly increased in 36 cryoglobulinemic patients with compared to those without active vasculitis (303 ± 208 vs 179 ± 62 pg/ml, respectively; p < 0.001; ANOVA). IFN-γ levels were significantly higher in MC+HCV than in controls [6.1 (range 0.8–114.5), 1.4 (range 0.7–2.4) pg/ml, respectively; p < 0.05; Mann-Whitney U test]. Serum TNF-α mean levels were significantly higher in MC+HCV than in controls [13.4 (range 1.8–369), 1.1 (range 0.7–3.2) pg/ml, respectively; p < 0.0001; Mann-Whitney U test]. A multiple regression analysis considering CXCL11 as a dependent variable, and age, alanine aminotransferase, IFN-γ, and TNF-α as independent variables, showed in MC+HCV patients a significant association only with IFN-γ (p < 0.0001).Conclusion.Our study demonstrates markedly high serum levels of CXCL11 in patients with MC+HCV compared to healthy controls overall in the presence of active vasculitis. A strong relationship between circulating IFN-γ and CXCL11 was shown, strongly supporting the role of a T helper 1 immune response in the pathogenesis of MC+HCV.

scholarly journals Alteration of Serum Levels of Cytokines in Schizophrenic Patients before and after Treatment with Risperidone

2019 ◽  
Author(s):  
Esfandiar Azizi ◽  
Ahmad Zavaran Hosseini ◽  
Sara Soudi ◽  
Ahmad Ali Noorbala
Keyword(s):  
Healthy Subjects ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Syndrome Scale ◽  
Tnf Α ◽  
Significant Difference ◽  
Before And After ◽  
Schizophrenic Patients ◽  
Ifn Γ

A growing body of evidence suggests the existence of abnormalities in the immune system of schizophrenic patients. The current study examined serum levels of interleukin (IL) -1β, IL-6, IL-2,interferon(IFN) -γ, and tumor necrosis factor(TNF)-α in schizophrenic patients before and after treatment with risperidone and correlated levels of these cytokines with symptomatology. The study group consisted of 24 schizophrenic patients as defined by Diagnostic and Statistical Manual of Mental Disorders 4th edition (DSM-IV) criteria and 24 healthy controls. Serum cytokine levels were examined using enzyme-linked immunosorbent assay (ELISA). Schizophrenic symptomatology was assessed with the Positive and Negative Syndrome Scale (PANSS) questionnaire. The serum levels of TNF-α, IL-1β and IL-6 were significantly higher in participants before treatment compared with the healthy controls and after treatment (p<0.001). IFN-γ and IL-2 levels were significantly lower in participants after treatment compared with before treatment and the healthy controls (p<0.001). Except for IL-6 (p<0.05), there was no significant difference in the levels of TNF-α and IL-1β between the patients receiving treatment and the healthy subjects. Moreover, there was no significant difference in levels of IFN-γ and IL-2 between patients before treatment and the healthy subjects. There were no significant correlations between the concentration of cytokines studied and the PANSS. Positive intercorrelations between the production of IFN-γ and IL-2 were detected for sums of all groups(r=0.33, p=0.005). Clinical improvement of treated patients was associated with a reduction in the studied cytokines. It seems that changes in the cytokines level may play a significant role in the psychopathology of these patients.

scholarly journals Clinical and Immunological Response to Doxycycline Versus Doxycycline Plus Vitamin C in Patients with Acne Vulgaris

2020 ◽  
Vol 11 (01) ◽  
pp. 09-11
Author(s):  
Zahraa I.J. Shubber ◽  
Entisar J. Al Mukhtar ◽  
Ifad K. Al-Shibly
Keyword(s):  
Vitamin C ◽  
Acne Vulgaris ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Significant Elevation ◽  
Once Daily ◽  
Severe Acne ◽  
Tnf Α ◽  
Ifn Γ

Objective: Study the influence of doxycycline versus doxycycline with vitamin C drugs in the management of acne vulgaris on some immunological parameters which include ( IL-1β, IL-8, IFN-γ, TNF-α, and TLR2) and the following objectives were adapted: 1-Selection of population for the study ( control, patients) groups and follow up the patients after one month of treatment, the first group receive doxycycline and the second group receive doxycycline with vitamin C,2- Blood samples collection and separation of serums for immunological analysis, 3- Statistical analysis. Methods: This study is a randomized clinical trial carried out in clinical dermatology in Merjan Medical City in Babylon from September 2018 to March 2019. The number of subjects enrolled in the present study was 60; their age was between (14- 30 years), among whom 30 were acne patients, the remaining 30 subjects were apparently healthy individuals, and they were served as control. A dermatologist diagnosed a total of 30 acne patients to having moderate to severe acne and divided into two groups (15 patients in each group). Patients in the first group were treated with doxycycline (100mg) once daily after meal for 30 days, while in the second group patients were treated with doxycycline (100mg) capsule once daily after a meal in combination with vitamin C (500mg) chewable tablets once daily. After 1-month of therapy, the response was evaluated clinically and immunologically by measure the concentration of pro-inflammatory cytokines (IL-8, IL-1β, IFN-γ, TNF-α) and ( TLR-2 ) by using enzyme-linked immunosorbent assay (ELISA) and the results were compared to their levels before treatment and that in the control group. Results: Significant elevation in the serum levels ( p ≤ 0.001) of immunological parameter IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2 among acne patients in comparison to the control. The clinical response in the first group was good, moderate and poor in 5 (33%), 7(47%) and 3(20%) respectively, while in the second group was good, moderate and poor in 7 (47%), 7(47%) and 1(6%) respectively, the immunological results showed that the serum levels to the (IL-1β, IL-8, IFN-γ, TNF-α) and (TLR-2 ) were more reduced in the second group compared to their levels in the first group. Conclusion: Significant elevation (p less than 0.001) in the serum levels of (IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2) among moderate to severe acne patients in comparison to control group. Clinically the combination of doxycycline plus vitamin C was more efficient as therapeutically in comparison to doxycycline alone. Immunologically doxycycline plus vitamin C was more effective in reducing serum levels of (IL-8, IL-1β, IFN-γ, TNF-α, and TLR-2) in comparison to doxycycline alone.

scholarly journals Serum Cytokine Responses in Primary Pneumonic Plague Patients

2010 ◽  
Vol 18 (1) ◽  
pp. 184-186 ◽  
Author(s):  
Xiaoyi Wang ◽  
Zuyun Wang ◽  
Zhaobiao Guo ◽  
Baiqing Wei ◽  
Fuzhang Tian ◽  
...  
Keyword(s):  
Time Course ◽  
Interleukin 2 ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Necrosis Factor Alpha ◽  
Pneumonic Plague ◽  
Tnf Α ◽  
Factor Alpha ◽  
Ifn Γ ◽  
Necrosis Factor

ABSTRACTThe serum levels of interleukin-2 (IL-2), gamma interferon (IFN-γ), tumor necrosis factor alpha (TNF-α), IL-4, IL-6, and IL-10 of pneumonic plague patients were determined by enzyme-linked immunosorbent assay. IL-6 was the only elevated cytokine in the patients, and its level increased with a clear time course, indicating that IL-6 might be a prognostic marker for predicting the progression of plague.

scholarly journals Carcinoembryonic Antigen: A Predictor of Inflammatory Condition in Patients with Allergic Bronchopulmonary Aspergillosis?

2021 ◽  
Author(s):  
Huan Ge ◽  
Feifei Yin ◽  
Yuanyuan Jiang ◽  
Lisha Luo ◽  
Shuanglinzi Deng ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Allergic Bronchopulmonary Aspergillosis ◽  
Elevated Serum ◽  
Serum Levels ◽  
Normal Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Gm Csf ◽  
Serum Cea ◽  
Ifn Γ

Abstract Background: Allergic bronchopulmonary aspergillus (ABPA) is a complex non-infectious pulmonary benign disease caused by immune response against to aspergillus. Carcinoembryonic antigen (CEA) is a tumor marker but also elevated in some benign diseases. Few studies on ABPA with elevated serum CEA levels have been reported. Objects: This study aims to comb the clinical characters of ABPA with elevated serum CEA. Methods: 20 patients diagnosed as ABPA were divided into two groups (CEA normal and CEA elevated) for retrospective analysis. The eosinophil count and ratio, IgE level in the pretherapy and post-treatment were compared. Serum samples of patients with ABPA (n = 20) and asthma (n = 20), healthy controls (n=20) were collected. Levels of cytokines (IL-4, IL-5, GM-CSF, IFN-γ) were analyzed by enzyme-linked immunosorbent assay. Results: We found that in ABPA patients with normal serum CEA levels, eosinophil counts and IgE levels decreased more obviously after treatment. Besides, we established higher serum levels of IL-4, IL-5, GM-CSF and IFN-γ in ABPA patients with elevated serum CEA levels.Conclusion: For the ABPA patients with elevated serum CEA levels, CEA may serve as a monitoring indicator for severity and treatment efficacy of ABPA.

scholarly journals Advanced glycation end product levels were correlated with inflammation and carotid atherosclerosis in type 2 diabetes patients

2020 ◽  
Vol 15 (1) ◽  
pp. 364-372 ◽  
Author(s):  
Jie Li ◽  
Haiyan Shangguan ◽  
Xiaoqian Chen ◽  
Xiao Ye ◽  
Bin Zhong ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Color Doppler ◽  
Enzyme Linked Immunosorbent Assay ◽  
Advanced Glycation ◽  
Advanced Glycation End Product ◽  
Inflammatory Status ◽  
Tnf Α ◽  
Ifn Γ

AbstractDiabetes mellitus with atherosclerosis (AS) adds to the social burden. This study aimed to investigate whether advanced glycation end product (AGE) levels were correlated with inflammation and carotid AS (CAS) in type 2 diabetes mellitus (T2DM) patients. A total of 50 elderly T2DM patients and 50 age-matched senior healthy subjects were recruited in this study. T2DM patients were classified into two groups based on the intima–media thickness (IMT) of the carotid artery from color Doppler ultrasonography. Patients with IMT > 1 mm were classified into the T2DM + CAS group (n = 28), and patients with IMT < 1 mm were assigned as the T2DM + non-atherosclerosis (NAS) group (n = 22). The plasma levels of AGEs, receptor for AGE (RAGE), tumor necrosis factor alpha (TNF-α), and interferon gamma (IFN-γ) of all subjects were measured by enzyme-linked immunosorbent assay. The T-lymphocyte subsets were analyzed by a flow detector. T2DM + CAS patients showed significantly higher concentrations of AGEs, RAGE, TNF-α, and IFN-γ in the peripheral blood. The highest levels of CD4+ T cells were observed in the T2DM + CAS group. The AGE level was positively correlated with the concentrations of RAGE, TNF-α, IFN-γ, and CD4+. In summary, the results showed that the levels of AGEs may be correlated with the inflammatory status in T2DM patients with CAS.

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