Optimization of Supercritical Fluid Extraction of Antioxidant Compounds from Venezuelan Rosemary Leaves

2012 ◽  
Vol 8 (4) ◽  
Author(s):  
Gabriela Caldera ◽  
Yalitza Figueroa ◽  
Maritza Vargas ◽  
Diego T. Santos ◽  
Germania Marquina-Chidsey
Keyword(s):  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Extraction Time ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Fluid Extraction ◽  
Static Extraction

Abstract The extraction of the antioxidant compounds from rosemary can be achieved by means of various processes, such as hydrodistillation, soxhlet extraction, microwave-accelerated hydrodistillation (MAHD) and supercritical fluid extraction (SFE). The aim of our study was to optimize SFE variables such as extraction pressure, extraction temperature and static extraction time for the maximum extraction of carnosol and carnosic acid from Venezuelan rosemary (Rosmarinus officinalis L.) leaves by using 23 full factorial and Box-Behnken designs and Response Surface Methodology (RSM). The chemical characterization of the supercritical fluid extracts was achieved by high-performance liquid chromatography (HPLC), and the antioxidant activity was determined by the peroxide index assay. For the extraction of carnosol, the variables of extraction temperature and static extraction time were statistically significant, and for the extraction of carnosic acid, only the extraction temperature-static extraction time interaction was significant. At a fixed pressure, the highest extraction of carnosol and carnosic acid were achieved when the operational extraction conditions of extraction temperature and static extraction time were set at 48°C for 69 min and 64°C for 57 min, respectively. SFE extracts presented higher antioxidant activities than commercial and soxhlet extracts, and pure dibutyl hidroxytoluene (BHT). Since the carnosic acid/carnosol ratios presented in our SFE extracts are higher than those from other countries, possibly it would be expected that higher antioxidant activity values would be obtained for the Venezuelan extracts.

scholarly journals Supercritical Fluid Extraction of Berry Seeds: Chemical Composition and Antioxidant Activity

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Graziele Gustinelli ◽  
Lovisa Eliasson ◽  
Cecilia Svelander ◽  
Thomas Andlid ◽  
Leif Lundin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Antioxidant Activity ◽  
Vitamin E ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antioxidant Compounds ◽  
Fluid Extraction ◽  
Black Currant

The influence of supercritical fluid extraction (SFE) and solvent extraction of oils from cloudberry, bilberry, and black currant seeds on the yield, chemical properties, and recovery of antioxidant compounds was investigated. SFE was performed for 1 h at 350 bar and at 50°C and 80°C. Fatty acids, vitamin E, carotenoids, and free radical-scavenging activity (DPPH) were assayed. SFE at 80°C resulted in higher oil yields for cloudberry and black currant seeds. The oils were rich in polyunsaturated fatty acids (PUFAs) (66.8%–75.9% w/w), with high percentages of linoleic and α-linolenic acids. The black currant seed extracts had the highest concentrations of vitamin E (range, 113.0–241.8 mg/100 g oil) and carotenoids (range, 11.5–32.3 mg/100 g oil) and the highest antioxidant activity. The cloudberry seed oils also had high antioxidant content and activity. These findings indicate the potential of SFE for the recovery of PUFA and antioxidant compounds in berry by-products.

Supercritical CO2Fluid Extraction of Imazaquin From Soil

Weed Science ◽  
1994 ◽  
Vol 42 (2) ◽  
pp. 249-253 ◽  
Author(s):  
Krishna N. Reddy ◽  
Martin A. Locke
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Extraction Method ◽  
Single Step ◽  
Extraction Temperature ◽  
Fluid Extraction ◽  
Spiked Soil ◽  
Dynamic Extraction ◽  
Conventional Extraction ◽  
Static Extraction

Feasibility of supercritical CO2fluid extraction of imazaquin from spiked soil (3.21 μmol kg-1) as an alternative to a conventional extraction method was investigated. The supercritical fluid extraction method involved single-step extraction of herbicide from soil with no further sample cleanup procedures. Extraction parameters were optimized for maximum herbicide recovery. Adding water as a modifier to air-dried soil significantly improved herbicide recovery. Extracting a 1-g soil sample with supercritical CO2at 0.80 g ml-1density and 3 ml min-1flow rate, 80 C extraction temperature, 6 min static extraction followed by 25 min dynamic extraction, and analyte trap temperature of 40 C was optimum for maximum herbicide recovery. When optimum supercritical fluid extraction conditions were used, imazaquin recovery from three texturally different soils ranged from 55 to 64%, which was comparable to a conventional extraction method (63%). The supercritical fluid extraction method consumed 4 ml methanol and 75 ml supercritical CO2and took approximately 1 h for sample extraction.

Effect of Static Extraction Time on Supercritical Fluid Extraction of Bioactive Compounds from Phyllanthus niruri

2020 ◽  
Vol 17 (2) ◽  
pp. 918-924 ◽  
Author(s):  
Norsyamimi Hassim ◽  
Masturah Markom ◽  
Masli Irwan Rosli ◽  
Shuhaida Harun
Keyword(s):  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Bioactive Compounds ◽  
Total Content ◽  
Operating Conditions ◽  
Extraction Time ◽  
Fluid Extraction ◽  
Active Components ◽  
Phyllanthus Niruri ◽  
Static Extraction

Supercritical fluid extraction (SFE) is an effective method to extract active components from plants. SFE process can be conducted by using a static or/and dynamic extraction. Static extraction is important for the diffusion of solvated analyte to the matrix surface. Therefore, the main objective of this study is to evaluate the static extraction time influence on SFE extraction of Phyllanthus niruri and the main bioactive compounds. The extraction was conducted by using supercritical carbon dioxide (SC-CO2) with 50% ethanol-water as a co-solvent at selected operating conditions (200 bar, 60 °C, 10% co-solvent content and 3 ml/min solvent total flow rate). The static extraction time was investigated in the range of 15 min–75 min by allowing the plant matrix to immerse in a mixture of SC-CO2 and 50% ethanol-water at the operating condition. The quantification of targeted compounds, which were gallic acid (GA), corilagin (CO) and ellagic acid (EA) were analysed by using high performance liquid chromatography (HPLC). The observation on the physical characteristics of the plant matrix was performed by using the scanning electron microscopy (SEM). The best result for total extraction yield was obtained at 60 min of static extraction time, which was 20.7% g/g sample. It also gave a better result for all three targeted compounds in terms of total content percentage (0.35% g GA/g extract, 3.05% g CO/g extract, and 5.17% g EA/g extract). In conclusion, static extraction time was crucial in extracting bioactive compounds in P. niruri by using SFE method and should be considered as the main parameter other than pressure and temperature.

Chemical profile and antioxidant activity of sage herbal dust extracts obtained by supercritical fluid extraction

2018 ◽  
Vol 120 ◽  
pp. 305-312 ◽  
Author(s):  
Branimir Pavlić ◽  
Oskar Bera ◽  
Nemanja Teslić ◽  
Senka Vidović ◽  
Giuseppina Parpinello ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Chemical Profile ◽  
Fluid Extraction

scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

Optimization of Supercritical Fluid Extraction of Phenolic Compounds from Peach Blossom (Amygdalus Persica) by Response Surface Methodology

2018 ◽  
Vol 17 (2) ◽  
pp. 180-187
Author(s):  
Yang Wen-Bo ◽  
Liu Jie-Chao ◽  
Liu Hui ◽  
Zhang Chun-Ling ◽  
Lv Zhen-Zhen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Phenolic Compounds ◽  
Response Surface ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Total Phenolic ◽  
Fluid Extraction ◽  
Phenolic Contents ◽  
C 30

Supercritical fluid extraction (SFE) was used for the extraction of phenolic compounds from peach blossom (Amygdalus persica). The optimum conditions of SFE were optimized by response surface methodology and confirmed at 64°C, 30 MPa, 143 min, and 35 mL ethanol (100%) as modifier for maximum total phenolic contents (54.10 mg GAE g−1 DW), which was higher than the yield achieved by ultrasonic-assisted extraction (44.04 mg GAE g−1 DW). The antioxidant activities and tyrosinase inhibitory activities of extraction extracted by SFE were 79.55% and 14.42%. Furthermore, chlorogenic acid, quercetin-3-O-galactoside, kaempferol-3-O-galactoside, quercitrin-3-O-rhamnoside, and kaempferol 4′-glucoside were identified in this study.

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