scholarly journals The Effects of Extraction Conditions on Extraction Yield and Syringin Content in Producing Standardized Tinospora crispa Aqueous Extract with High Antioxidant Activity

2021 ◽  
pp. 106-120
Author(s):  
Zamree Md Shah ◽  
Mohd Kamal Nik Hasan ◽  
Khairul Kamilah Abdul Kadir ◽  
Mohd Shahidan Mohd Arshad ◽  
Zulkhairi Amom
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Activities ◽  
Pharmaceutical Preparations ◽  
Extraction Yield ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Solid Ratio ◽  
Beneficial Effects ◽  
High Antioxidant Activity ◽  
Optimized Conditions

The herbal plant known as Tinospora crispa is reported to have many beneficial effects on health and has great potential in future to be developed as a health product either in the form of traditional medicine, food supplements or in pharmaceutical preparations. However, so far knowledge on processing procedures to produce quality standardized extracts of this plant to be used in product development has still not widely reported. Therefore, the objective of this study was to determine the optimal extraction conditions for producing a standardized T. crispa aqueos extract (STCAE) with high extraction yield and high syringin content. Experiments were conducted to determine the effects of various extraction conditions involving temperature (25–100 ° C), extraction time (0.5–6 hours) and liquid (water) to solid ratio (5: 1–25: 1 ml of solvent per g stem dry). Using optimized conditions obtained, the extract was standardized based on syringin and was further investigated on its antioxidant activity through DPPH, FRAP and TBA bioassays. Results revealed that the optimum extraction conditions were found to be 1 h extraction time and 15:1 ml g−1 liquid-to-solid ratio. For the extraction temperature, 60°C was found to be the best. STCAE was produced on the basis of the extract to contain with at least 0.4 wt% of syringin. STCAE was found to possess high antioxidant activities through DPPH, FRAP and TBA bioassays.

Effects of Extraction Conditions on Antioxidant Activities of Roselle (Hibiscus sabdariffa L.) Extracts

2020 ◽  
Vol 977 ◽  
pp. 201-206 ◽  
Author(s):  
Quoc Duy Nguyen ◽  
Tri Nhut Pham ◽  
Mai Le Thanh Binh ◽  
Ma Thuan ◽  
Ngo Thi Thanh Van ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radical ◽  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Extraction Time ◽  
Solvent Ratio ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Solid Ratio

The objective of this study was to determine the optimal value of factors affecting the extraction of antioxidant activity of Roselle calyces. The investigated factors included extraction temperatures (30, 40, 50, 60, 70 and 80°C), extraction time (30, 60, 90, 120 and 150 minutes) and solid to solvent ratio (1:5, 1:10, 1:20, 1:40 and 1:50 g/mL). The efficiency of extraction was evaluated based on antioxidant activities which were measured by DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging assay, ferric reducing antioxidant power assay (FRAP). The results showed that when the extraction temperature range of 70–80°C, DPPH free radical scavenging and FRAP were highest with 677.47–725.81 μmolTE/L and 4186.91–4391.62 μmolTE/L, respectively. For the extraction time from 120 to 150 minutes, the resulting extracts with highest FRAP and DPPH free radical scavenging antioxidant capacity were obtained. Meanwhile, the antioxidant activity tended to decrease as the solvent/solid ratio increased.

scholarly journals Total phenolic content and antioxidant activity of sesame cake aqueous extracts

2018 ◽  
Vol 17 (06) ◽  
pp. 77-84
Author(s):  
Tuyen C. Kha
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antioxidant Activities ◽  
Extraction Yield ◽  
Major Influence ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Aqueous Extracts ◽  
Sesame Cake

The objective of this study was to investigate the efficiency of phenolic extraction and antioxidant activity from sesame cake using water extraction method and to evaluate the possibility of employing microwave irradiation to improve the extraction yield. The result showed that extraction temperature had major influence on total phenolic content and antioxidant activities of the extracts, whereas extraction time was found to be insignificant. The optimum extraction condition recommended were 90oC for 30 min in this research. Furthermore, microwave pre-treatment at 120 s could have significantly positive influence on the overall extraction yield, especially the total phenolics and antioxidants based on FRAP assay. Therefore, the obtained results suggest that sesame aqueous extracts could be a source of antioxidants with more feasible applications in food as well as other industries.

Effect of Extraction Process on Extraction Yeild, Total Polyphenol Content and Antioxidant Activity of Jasminum Subtriplinerve

2019 ◽  
Vol 814 ◽  
pp. 527-531
Author(s):  
Trieu Tuan Anh ◽  
Luu Tu Hao ◽  
Long Giang Bach ◽  
Duy Chinh Nguyen ◽  
Tri Duc Lam
Keyword(s):  
Antioxidant Activity ◽  
Extraction Process ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Total Phenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Herbal Plants ◽  
Optimized Conditions

Jasminum Subtriplinerve Blume (Oleaceae) is herbal plants widely a tea for weight loss, stimulates milk glands. Extract product it easy used yet had little research. This study was carried out to evaluate the effect of extracting conditions such as water/material ratio, extraction temperature and extracting time on extracting yield, polyphenol content and antioxidant activity of J.subtriplinerve extract. The total phenolic compounds were determined according to the Folin–Ciocalteu method. Extraction yield (0.22g/100 g) was obtained at water/material ratio 15:1 (ml/g), 40°C and 4 hours extraction time. Under these optimized conditions, the polyphenol content of was 2640.4 μg/1g extract. And the extract can harvest 46.11 % of DPPH (30 μg/ mL) at 10 000 μg/ml concentration

scholarly journals Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Processes ◽  
2019 ◽  
Vol 7 (11) ◽  
pp. 854
Author(s):  
Jichang Li ◽  
Rui Wang ◽  
Zunlai Sheng ◽  
Zhiyong Wu ◽  
Chunli Chen ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Chlorogenic Acid ◽  
Response Surface ◽  
Extraction Process ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Single Factor ◽  
Solid Ratio ◽  
Scutellariae Radix ◽  
Optimized Conditions

In this study, a simultaneous water extraction process for baicalin, wogonoside, and chlorogenic acid has been optimized. The effect of extraction temperature, extraction time, and liquid–solid ratio was scrutinized by single factor experiments and further analyzed by Box–Behnken design (BBD) approach using response surface methodology (RSM). The extraction yield of investigated compounds was determined by high performance liquid chromatography (HPLC). Single-factor experiments and response surface analysis results revealed that the optimized conditions are: Liquid to solid ratio 25:1 (mL/g), extraction temperature 93 °C, extraction time 2.4 h, and the extraction cycle two. Importantly, it has been noted that under the above conditions, concentrations of baicalin, wogonoside, and chlorogenic were 0.078, 0.031, and 0.013 mg/mL, respectively, and the overall desirability (OD) value was 0.76 which was higher than the non-optimized conditions and the deviation from the predicted OD value was only 2.44%. Conclusively, it has been suggested that the model was stable and feasible, and fit for extraction of baicalin, wogonoside, and chlorogenic acid from Scutellariae Radix and Lonicerae (L.) japonicae Flos.

Optimization of Supercritical Fluid Extraction of Antioxidant Compounds from Venezuelan Rosemary Leaves

2012 ◽  
Vol 8 (4) ◽  
Author(s):  
Gabriela Caldera ◽  
Yalitza Figueroa ◽  
Maritza Vargas ◽  
Diego T. Santos ◽  
Germania Marquina-Chidsey
Keyword(s):  
Antioxidant Activity ◽  
Supercritical Fluid Extraction ◽  
Supercritical Fluid ◽  
Antioxidant Activities ◽  
Extraction Time ◽  
Carnosic Acid ◽  
Antioxidant Compounds ◽  
Extraction Temperature ◽  
Fluid Extraction ◽  
Static Extraction

Abstract The extraction of the antioxidant compounds from rosemary can be achieved by means of various processes, such as hydrodistillation, soxhlet extraction, microwave-accelerated hydrodistillation (MAHD) and supercritical fluid extraction (SFE). The aim of our study was to optimize SFE variables such as extraction pressure, extraction temperature and static extraction time for the maximum extraction of carnosol and carnosic acid from Venezuelan rosemary (Rosmarinus officinalis L.) leaves by using 23 full factorial and Box-Behnken designs and Response Surface Methodology (RSM). The chemical characterization of the supercritical fluid extracts was achieved by high-performance liquid chromatography (HPLC), and the antioxidant activity was determined by the peroxide index assay. For the extraction of carnosol, the variables of extraction temperature and static extraction time were statistically significant, and for the extraction of carnosic acid, only the extraction temperature-static extraction time interaction was significant. At a fixed pressure, the highest extraction of carnosol and carnosic acid were achieved when the operational extraction conditions of extraction temperature and static extraction time were set at 48°C for 69 min and 64°C for 57 min, respectively. SFE extracts presented higher antioxidant activities than commercial and soxhlet extracts, and pure dibutyl hidroxytoluene (BHT). Since the carnosic acid/carnosol ratios presented in our SFE extracts are higher than those from other countries, possibly it would be expected that higher antioxidant activity values would be obtained for the Venezuelan extracts.

scholarly journals Reflux Extraction Optimization and Antioxidant Activity of Phenolic Compounds from Pleioblastus amarus (Keng) Shell

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 362
Author(s):  
Yuan Ma ◽  
Ailian Meng ◽  
Ping Liu ◽  
Yuanyuan Chen ◽  
Anqi Yuan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Ethanol Concentration ◽  
Extraction Time ◽  
Composition Analysis ◽  
Extraction Temperature ◽  
Factors Affecting ◽  
Solid Ratio ◽  
Ic50 Values ◽  
Phenol Composition

Phenols were extracted from the Pleioblastus amarus (Keng) shell (PAS) using ethanol. A Plackett–Burman assessment indicated that the factors affecting polyphenol extraction included the ethanol concentration, extraction temperature, liquid to solid ratio, extraction time, and reflux extraction times; the best extraction parameters were the ethanol concentration of 75%, a 20:1 liquid to solid ratio, and an extraction time of 2.1 h. The number of polyphenols was 7.216 mg/g. Furthermore, the phenol composition analysis showed the presence of p-Coumaric acid (196.88 mg /mL) and rutin (312.9 mg /mL), which were used for the in vitro extraction and determination of the antioxidant activity. According to the A, B, C, and D antioxidant activity assays, the ethyl acetate phase was the strongest with low IC50 values of 0.169 ± 0.01 mg/mL, 0.289 ± 0.01 mg/mL, 0.372 ± 0.01 mg/mL, and 1.029 ± 0.03 mg/mL, respectively, confirming high antioxidant activity. For the n-butanol and petroleum ether phases, antioxidant activity was lower. This study showed that the polyphenol extract from Pleioblastus amarus (Keng) shell displayed excellent antioxidant activity, enhancing its practical application.

scholarly journals Optimization of extraction of total flavonoids from Drynaria rhizome, and its effect on osteoclast differentiation

2021 ◽  
Vol 18 (4) ◽  
pp. 829-835
Author(s):  
Bo Yuan ◽  
Fang Yu ◽  
WenJi Song ◽  
ZhengNan Zhao ◽  
HaiDong Liang
Keyword(s):  
Bone Resorption ◽  
Osteoclast Differentiation ◽  
Cathepsin K ◽  
Extraction Yield ◽  
Bone Diseases ◽  
Extraction Time ◽  
Total Flavonoids ◽  
Extraction Temperature ◽  
Solid Ratio

Purpose: To optimize the extraction parameters for total flavonoids of Drynaria rhizome, and determine their effect on osteoclast differentiation and bone resorption in vitro. Methods: A 5-level 3-factor central composite design was applied to the optimization of extract yield of total flavonoids from Drynaria rhizome. Response Surface Methodology (RSM) design was used to optimize total flavonoids extraction from Drynaria rhizome. The independent factors included extraction temperature (A), extraction time (B) and liquid: solid ratio (C). Moreover, NFATc1, DC-STAMP, cathepsin K and MMP-9 mRNA expressions were determined. Results: Values obtained were fitted into a second-order polynomial equation with multiple regression analysis using a statistical method. Analysis of variance results indicate that the independent variables influenced total flavonoid extraction from Drynaria rhizome. The optimal conditions for extraction yield were extraction temperature of 75 oC, extraction time of 100 min, and liquid: solid ratio of 107:1. The yield of 5.38 ± 0.62 % was consistent with these optimized conditions, which was an indication of the accuracy of the model. Experiments revealed that total flavonoids from Drynaria rhizome regulated the expression levels of NFATc1, DC-STAMP, cathepsin K and MMP-9 mRNA Conclusion: This study has successfully optimized the extraction yield of total flavonoids from Drynaria rhizome. The total flavonoids inhibit osteoclast differentiation and bone resorption. Thus, they may be beneficial in the treatment of bone diseases.

Optimization of Extraction of Tannins from Banana Peel Using Response Surface Methodology

2014 ◽  
Vol 678 ◽  
pp. 566-571
Author(s):  
Ting Ru Wu ◽  
Hong Ling Wang ◽  
Shi Wen Jiang ◽  
Dan Dan Liu ◽  
Fei Wei
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Ethanol Concentration ◽  
Extraction Yield ◽  
Extraction Time ◽  
Banana Peel ◽  
Extraction Temperature ◽  
Liquid Ratio ◽  
Optimized Conditions ◽  
Tannin Extraction

Response surface methodology (RSM) was employed to optimize the extraction conditions of tannins from banana peels. Based on the single-factor experiments, four independent variables including ethanol concentration, solid to liquid ratio, extraction time and extraction temperature were selected as affecting factors. According to central composite design (CCD) the four factor, five levels experiments were carried out with the tannin extraction yield as evaluation index. The results show that the optimized conditions are as follows: ethanol concentration of 75.44%, solid to liquid ratio of 1:35.34, extraction time of 7.94 h, extraction temperature of 62.85°C. Under the optimized conditions, the predicted tannin extraction yield reaches 58.55%, while the actual extraction yield is 57.42% with the relative error of 1.13%.

scholarly journals Optimization of Extraction Conditions to Improve Phenolic Content and In Vitro Antioxidant Activity in Craft Brewers’ Spent Grain Using Response Surface Methodology (RSM)

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1398
Author(s):  
Ana Isabel Andres ◽  
Maria Jesus Petron ◽  
Ana Maria Lopez ◽  
Maria Luisa Timon
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Simple Method ◽  
Solid Ratio ◽  
Spent Grain ◽  
G Ratio ◽  
In Vitro Antioxidant Activity

Extraction temperature, extraction time and liquid (water) to solid ratio were optimized in order to extract antioxidant phenolic compounds from brewers’ spent grain (BSG). The extracts were analysed for their total phenol content (TPC) and antioxidant activity was measured using three different methods: 2,2-diphenyl-2-picrylhydrazyl (DPPH) free radical, 2,2′-azino-bis(3-ethylbenothiazoline-6-sulphonic acid) (ABTS), and reducing power (RP) assays. All the parameters except extraction time promoted different efficiencies for the extraction of antioxidant phenolic compounds. TPC extraction was higher at lower temperatures and lower liquid/solid ratios up to a certain point. In this sense, a decrease in TPC with increasing liquid/solid ratios took place until a 16:1 ratio and a plateau was reached beyond that ratio. The highest DPPH activity was reported for 30–35 °C and 60–90 min extraction and 60–90 min extraction with a 25 mL/g ratio. ABTS values increased as the liquid to solid ratio decreased, being positively correlated with TPC (R = 0.788; p < 0.01). The highest RP was achieved at 30–33 °C extraction temperature and 10–14 mg/mL v/w ratio and at 116–120 min extraction and 16–17 mg/mL ratio. Gallic acid accounted for the majority of the phenolic compounds found, followed by hydroxyphenylacetic acid, epicatechin, and protocatechuic acid. Sinapic, 4-hydroxy benzoic, and syringic acids were also found in lower quantities. Coumaric, vanillic, ferulic, and caffeic acids were present in very small amounts. All the extracts contained phenolics and showed in vitro antioxidant activity, but the extracts obtained by using 30 °C, 121.9 min, and 10 mL/g liquid/solid ratio exhibited the highest content in TPC and antioxidant potential. The aqueous extraction of a potentially bioactive extract from BSG was demonstrated to be an efficient and simple method to recover these value-added compounds.

scholarly journals Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Biomedicines ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 294
Author(s):  
Raffaele Pugliese ◽  
Anna Arnoldi ◽  
Carmen Lammi
Keyword(s):  
Mechanical Properties ◽  
Antioxidant Activity ◽  
Self Assembly ◽  
Antioxidant Activities ◽  
Functional Materials ◽  
Cd Spectroscopy ◽  
Beneficial Effects ◽  
Naturally Occurring ◽  
New Research ◽  
Peptide Hydrogel

Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

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