Chemically induced mutations for male sterility in Petunia x hybrida

ABSTRACT The fimbria-associated MrkD1P protein mediates adherence of type 3 fimbriate strains of Klebsiella pneumoniae to collagen type V. Currently, three different MrkD adhesins have been described in Klebsiella species, and each possesses a distinctive binding pattern. Therefore, the binding abilities of mutants possessing defined mutations within themrkD 1P gene were examined in order to determine whether specific regions of the adhesin molecule were responsible for collagen binding. Both site-directed and chemically induced mutations were constructed within mrkD 1P, and the ability of the gene products to be incorporated into fimbrial appendages or bind to collagen was determined. Binding to type V collagen was not associated solely with one particular region of the MrkD1Pprotein, and two classes of nonadhesive mutants were isolated. In one class of mutants, the MrkD adhesin was not assembled into the fimbrial shaft, whereas in the second class of mutants, the adhesin was associated with fimbriae but did not bind to collagen. Both hemagglutinating and collagen-binding activities were associated with the MrkD1P molecule, since P pili and type 3 fimbriae carrying adhesive MrkD proteins exhibited identical binding properties.

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Chemically induced mutations in a MutaMouse reporter gene inform mechanisms underlying human cancer mutational signatures

Communications Biology ◽

10.1038/s42003-020-01174-y ◽

2020 ◽

Vol 3 (1) ◽

Cited By ~ 2

Author(s):

Marc A. Beal ◽

Matthew J. Meier ◽

Danielle P. LeBlanc ◽

Clotilde Maurice ◽

Jason M. O’Brien ◽

...

Keyword(s):

Human Cancer ◽

Published Data ◽

Induced Mutations ◽

Lung Cancers ◽

Human Cancers ◽

Chemically Induced ◽

Mutation Pattern ◽

Environmental Causes ◽

Next Generation Sequencing Ngs

AbstractTransgenic rodent (TGR) models use bacterial reporter genes to quantify in vivo mutagenesis. Pairing TGR assays with next-generation sequencing (NGS) enables comprehensive mutation pattern analysis to inform mutational mechanisms. We used this approach to identify 2751 independent lacZ mutations in the bone marrow of MutaMouse animals exposed to four chemical mutagens: benzo[a]pyrene, N-ethyl-N-nitrosourea, procarbazine, and triethylenemelamine. We also collected published data for 706 lacZ mutations from eight additional environmental mutagens. We report that lacZ gene sequencing generates chemical-specific mutation signatures observed in human cancers with established environmental causes. For example, the mutation signature of benzo[a]pyrene, a carcinogen present in tobacco smoke, matched the signature associated with tobacco-induced lung cancers. Our results suggest that the analysis of chemically induced mutations in the lacZ gene shortly after exposure provides an effective approach to characterize human-relevant mechanisms of carcinogenesis and propose novel environmental causes of mutation signatures observed in human cancers.

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Whole genome profiling of spontaneous and chemically induced mutations in Toxoplasma gondii

BMC Genomics ◽

10.1186/1471-2164-15-354 ◽

2014 ◽

Vol 15 (1) ◽

pp. 354 ◽

Cited By ~ 23

Author(s):

Andrew Farrell ◽

Bradley I Coleman ◽

Brian Benenati ◽

Kevin M Brown ◽

Ira J Blader ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Whole Genome ◽

Induced Mutations ◽

Genome Profiling ◽

Chemically Induced ◽

Whole Genome Profiling

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Characterization of chemically induced mutations in the ad-1 locus of Schizosaccharomyces pombe

Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis ◽

10.1016/0027-5107(73)90017-1 ◽

1973 ◽

Vol 18 (1) ◽

pp. 15-24 ◽

Cited By ~ 6

Author(s):

E. Segal ◽

P. Munz ◽

U. Leupold

Keyword(s):

Schizosaccharomyces Pombe ◽

Induced Mutations ◽

Chemically Induced

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Expression of chemically induced mutations in mammalian cells using an in situ procedure

Mutation Research/Environmental Mutagenesis and Related Subjects ◽

10.1016/0165-1161(92)91174-p ◽

1992 ◽

Vol 271 (2) ◽

pp. 152-153

Author(s):

D. Daston ◽

K. Hines ◽

C. Rudd ◽

W. Caspary

Keyword(s):

Mammalian Cells ◽

Induced Mutations ◽

Chemically Induced

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Enhanced susceptibility to chemically induced colitis caused by excessive endosomal TLR signaling in LRBA-deficient mice

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.1901407116 ◽

2019 ◽

Vol 116 (23) ◽

pp. 11380-11389 ◽

Cited By ~ 1

Author(s):

Kuan-wen Wang ◽

Xiaoming Zhan ◽

William McAlpine ◽

Zhao Zhang ◽

Jin Huk Choi ◽

...

Keyword(s):

Molecular Mechanisms ◽

Intestinal Inflammation ◽

Type I ◽

Induced Mutations ◽

Tlr Signaling ◽

Nonsense Mutations ◽

Chemically Induced ◽

Inflammatory Bowel ◽

Deficient Mice ◽

Stimulation Of

LPS-responsive beige-like anchor (LRBA) protein deficiency in humans causes immune dysregulation resulting in autoimmunity, inflammatory bowel disease (IBD), hypogammaglobulinemia, regulatory T (Treg) cell defects, and B cell functional defects, but the cellular and molecular mechanisms responsible are incompletely understood. In an ongoing forward genetic screen forN-ethyl-N-nitrosourea (ENU)-induced mutations that increase susceptibility to dextran sodium sulfate (DSS)-induced colitis in mice, we identified two nonsense mutations inLrba. Although Tregcells have been a main focus in LRBA research to date, we found that dendritic cells (DCs) contribute significantly to DSS-induced intestinal inflammation in LRBA-deficient mice.Lrba−/−DCs exhibited excessive IRF3/7- and PI3K/mTORC1-dependent signaling and type I IFN production in response to the stimulation of the Toll-like receptors (TLRs) 3, TLR7, and TLR9. Substantial reductions in cytokine expression and sensitivity to DSS in LRBA-deficient mice were caused by knockout ofUnc93b1, a chaperone necessary for trafficking of TLR3, TLR7, and TLR9 to endosomes. Our data support a function for LRBA in limiting endosomal TLR signaling and consequent intestinal inflammation.

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Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.)

Canadian Journal of Plant Science ◽

10.4141/cjps2012-280 ◽

2013 ◽

Vol 93 (4) ◽

pp. 675-681 ◽

Cited By ~ 19

Author(s):

Qing Song Ba ◽

Gai Sheng Zhang ◽

Jun Sheng Wang ◽

Hui Xue Che ◽

Hong Zhan Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Triticum Aestivum ◽

Male Sterility ◽

Reactive Oxygen ◽

Triticum Aestivum L ◽

Oxygen Species ◽

Ros Scavenging ◽

Male Sterile ◽

Chemically Induced

Ba, Q. S., Zhang, G. S., Wang, J. S., Che, H. X., Liu, H. Z., Niu, N., Ma, S. C. and Wang, J. W. 2013. Relationship between metabolism of reactive oxygen species and chemically induced male sterility in wheat (Triticum aestivum L.). Can. J. Plant Sci. 93: 675–681. Chemically induced male sterility (CIMS) systems in wheat are among the male sterility types used for hybrid wheat (Triticum aestivum L.) production in China. Some studies suggested that male sterile line Xi'nong 1376-CIMS induced by chemical hybridizing agents (CHA) may suffer from oxidative stress as its cyanide-resistant respiration is lower than that of Xi'nong1376. To elucidate the metabolic mechanism of reactive oxygen species (ROS) in the CIMS anthers, the metabolism changes in the production and scavenging of ROS and gene expression related to ROS-scavenging enzymes were investigated in the anther of Xi'nong 1376-CIMS and Xi'nong1376.Anthers of Xi'nong 1376-CIMS had higher contents of [Formula: see text] and H2O2 than those of 1376, which corresponds to expression level of the NADPH oxidase (NOX) gene, and has higher contents of malondialdehyde compared with 1376. Simultaneously, there were lower activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascrodate peroxidase (APX) in scavenging ROS in the anthers of the Xi'nong 1376-CIMS line than in Xi'nong1376. Meanwhile, the expressions of SOD, POD, CAT and APX genes in 1376 were always higher at different levels than those in the Xi'nong 1376-CIMS line except for POD in stage 1. Therefore, it is possible that the sterility in Xi'nong 1376-CIMS is related to the abortion of microspores induced by chronic oxidative stress caused by an abnormal increase in ROS.

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