Characterization of the different lectins from Viscum album (misteltoe) and their structural relationships with the agglutinins from Abrus precatorius and Ricinus communis

Lectin histochemistry was used to perform in situ characterization of the glycoconjugates present in boar testis and epididymis. Thirteen horseradish peroxidase- or digoxigenin-labelled lectins were used in samples obtained from healthy fertile boars. The acrosomes of the spermatids were stained intensely by lectins with affinity for galactose and N-acetyl-galactosamine residues, these being soybean, peanut and Ricinus communis agglutinins. Sertoli cells were stained selectively by Maackia ammurensis agglutinin. The lamina propria of seminiferous tubules showed the most intense staining with fucose-binding lectins. The Golgi area and the apical part of the principal cells of the epididymis were stained intensely with many lectins and their distribution was similar in the three zones of the epididymis. On the basis of lectin affinity, both testis and epididymis appear to have N- and O-linked glycoconjugates. Spermatozoa from different epididymal regions showed different expression of terminal galactose and N-acetyl-galactosamine. Sialic acid (specifically alpha2,3 neuraminic-5 acid) was probably incorporated into spermatozoa along the extratesticular ducts. These findings indicate that the development and maturation of boar spermatozoa are accompanied by changes in glycoconjugates. As some lectins stain cellular or extracellular compartments specifically, these lectins could be useful markers in histopathological evaluation of diseases of boar testis and epididymis.

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Isolation and Comparison of Galactose-binding Lectins from Abrus precatorius and Ricinus communis

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)43000-7 ◽

1974 ◽

Vol 249 (3) ◽

pp. 803-810 ◽

Cited By ~ 3

Author(s):

Sjur Olsnes ◽

Erling Saltvedt ◽

Alexander Pihl

Keyword(s):

Ricinus Communis ◽

Abrus Precatorius

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Biodiesel Production from Melia azedarach and Ricinus communis Oil by Transesterification Process

Catalysts ◽

10.3390/catal10040427 ◽

2020 ◽

Vol 10 (4) ◽

pp. 427 ◽

Cited By ~ 3

Author(s):

Muhammad Awais ◽

Sa’ed A Musmar ◽

Faryal Kabir ◽

Iram Batool ◽

Muhammad Asif Rasheed ◽

...

Keyword(s):

Ricinus Communis ◽

Cost Effective ◽

Biodiesel Production ◽

Molar Ratio ◽

Melia Azedarach ◽

Renewable Fuel ◽

Animal Fats ◽

Edible Crops ◽

American Society

Biodiesel is a renewable fuel usually produced from vegetable oils and animal fats. This study investigates the extraction of oil and its conversion into biodiesel by base-catalyzed transesterification. Firstly, the effect of various solvents (methanol, n-hexane, chloroform, di-ethyl ether) on extraction of oil from non-edible crops, such as R. communis and M. azedarach, were examined. It was observed that a higher concentration of oil was obtained from R. communis (43.6%) as compared to M. azedarach (35.6%) by using methanol and n-hexane, respectively. The extracted oils were subjected to NaOH (1%) catalyzed transesterification by analyzing the effect of oil/methanol molar ratio (1:4, 1:6, 1:8 and 1:10) and varying temperature (20, 40, 60 and 80 °C) for 2.5 h of reaction time. M. azedarach yielded 88% and R. communis yielded 93% biodiesel in 1:6 and 1:8 molar concentrations at ambient temperature whereas, 60 °C was selected as an optimum temperature, giving 90% (M. azedarach) and 94% (R. communis) biodiesel. The extracted oil and biodiesel were characterized for various parameters and most of the properties fulfilled the American Society for Testing and Materials (ASTM) standard biodiesel. The further characterization of fatty acids was done by Gas Chromatography/Mass Spectrometer (GC/MS) and oleic acid was found to be dominant in M. azedarach (61.5%) and R. communis contained ricinoleic acid (75.53%). Furthermore, the functional groups were analyzed by Fourier Transform Infrared Spectroscopy. The results suggested that both of the oils are easily available and can be used for commercial biodiesel production at a cost-effective scale.

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Extraction, purification, and biochemical characterization of serine protease from leaves of Abrus precatorius

Preparative Biochemistry & Biotechnology ◽

10.1080/10826068.2017.1373289 ◽

2017 ◽

Vol 47 (10) ◽

pp. 1016-1024 ◽

Cited By ~ 3

Author(s):

Ngangoum Eric Serge ◽

Mezajoug Kenfack Laurette Blandine ◽

Sanjit Kumar ◽

Tchiégang Clergé ◽

Mookambeswaran Vijayalakshmi

Keyword(s):

Serine Protease ◽

Biochemical Characterization ◽

Abrus Precatorius

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Melliferous flora and pollen characterization of honey samples of Apis mellifera L., 1758 in apiaries in the counties of Ubiratã and Nova Aurora, PR

Anais da Academia Brasileira de Ciências ◽

10.1590/s0001-37652013005000017 ◽

2013 ◽

Vol 85 (1) ◽

pp. 307-326 ◽

Cited By ~ 8

Author(s):

ELIZABETE S. SEKINE ◽

VAGNER A.A. TOLEDO ◽

MARCELO G. CAXAMBU ◽

SUZANE CHMURA ◽

ELIZA H. TAKASHIBA ◽

...

Keyword(s):

Glycine Max ◽

Apis Mellifera ◽

Native Species ◽

Ricinus Communis ◽

Cultivated Plants ◽

Melia Azedarach ◽

Native Vegetation ◽

Pollen Types ◽

Pollen Analyses

The aim of this study was to carry out a survey of the flora with potential for beekeeping in the counties of Ubiratã and Nova Aurora-PR through the collection of plants and pollen analyses in honey samples collected monthly. 208 species of plants were recorded, distributed in 66 families. The families that showed the major richness of pollen types were: Asteraceae, Myrtaceae and Solanaceae. Approximately 80 pollen types were found in honey samples, most of them were characterized as heterofloral. Cultivated plants, such as Glycine max (soybean) and Eucalyptus spp., were representative in some months of the year. Exotic species, such as Ricinus communis and Melia azedarach, were also frequent. However, over than 50% of the pollen types belong to native species of the region, such as Schinus terebinthifolius, Baccharis spp. Alchornea triplinervia, Parapiptadenia rigida, Hexaclamys edulis, Zanthoxylum sp. and Serjania spp., indicating the importance of the native vegetation for the survival of the colonies.

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Structure Characterization of Sulfate and Oxysulfate Phases Formed During Sulfuric Acid Digestion of Ilmenites

Australian Journal of Chemistry ◽

10.1071/ch9960801 ◽

1996 ◽

Vol 49 (7) ◽

pp. 801 ◽

Cited By ~ 8

Author(s):

IE Grey ◽

MR Lanyon ◽

R Stranger

Keyword(s):

Sulfuric Acid ◽

Rietveld Method ◽

Three Dimensional ◽

Structure Characterization ◽

Acid Digestion ◽

X Ray Diffraction ◽

Titanium Sulfate ◽

Structural Relationships ◽

Concentrated Sulfuric Acid

Laboratory digestion of natural and upgraded ilmenites with concentrated sulfuric acid has been carried out under conditions simulating the sulfate-route pigment process. X-Ray diffraction studies on the solid digestion cakes led to the characterization of a number of different iron titanium sulfate and oxysulfate compounds, whose formation was dependent on the ilmenite composition and digestion conditions. Two different sulfate phases were identified, with structures related to those for Fe2(SO4)3 and H2O[ Zr (HPO4)2]. Four different iron titanium oxysulfates , ( Fe,Ti )(O,OH)SO4, were identified as major digestion products, three having structures related to those for β-NbOPO4, GeOHPO4 and lazulite , Mg[AlOHPO4], and the fourth being a new struture type. The structures of the oxysulfate phases were refined by the Rietveld method. Both the sulfates and the oxysulfates have three-dimensional framework structures formed by corner linking of ( Ti,Fe )O6 octahedra and SO4 tetrahedra, and containing channels or interlayer regions that can be occupied by cations such as H3O+ and Fe2+. The different structures have many features in common. Their structural relationships are discussed and mechanisms are proposed for the phase transformations encountered in the digestion studies.

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The multigene family of lysophosphatidate acyltransferase (LPAT)-related enzymes in Ricinus communis. Cloning and molecular characterization of two LPAT genes that are expressed in castor seeds

Plant Science ◽

10.1016/j.plantsci.2012.09.015 ◽

2013 ◽

Vol 199-200 ◽

pp. 29-40 ◽

Cited By ~ 38

Author(s):

José María Arroyo-Caro ◽

Tarik Chileh ◽

Michael Kazachkov ◽

Jitao Zou ◽

Diego López Alonso ◽

...

Keyword(s):

Molecular Characterization ◽

Multigene Family ◽

Ricinus Communis ◽

Castor Seeds

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IMUNIDADE CRUZADA PELAS SEMENTES DE Abrus precatorius E Ricinus communis EM BOVINOS

Pesquisa Veterinária Brasileira ◽

10.1590/s0100-736x1997000100005 ◽

1997 ◽

Vol 17 (1) ◽

pp. 25-35 ◽

Cited By ~ 6

Author(s):

Carlos Hubinger Tokarnia ◽

Jürgen Döbereiner

Keyword(s):

Ricinus Communis ◽

Abrus Precatorius ◽

Ricinus Communis L

Cinco bovinos imunizados contra a ação tóxica das sementes de Abrus precatorius L. ("tento", "jiquiriti") não adoeceram ou somente levemente pela administração das sementes de Ricinus communis L. ("mamona"), em doses que em bovinos que antes nunca ingeriram sementes de A. precatorius ou R. communis, causaram intoxicação de intensidade de grau moderado a acentuado ou até a morte. Um sexto bovino, que não ficou bem imunizado contra a ação tóxica das sementes de A. precatorius, adoeceu em grau acentuado pela administração de dose elevada das sementes de R. communis. Já dos cinco bovinos imunizados contra a ação tóxica das sementes de R. communis quatro adoeceram em grau acentuado, oquinto em grau moderado, pela administração das sementes de A. precatorias em doses que em bovinos que antes nunca ingeriram sementes de R. communis ou A. precatorius causaram intoxicação de intensidade leve a acentuada. Estes resultados permitem concluir que bovinos imunizados contra a ação tóxica das sementes de A. precatorius são resistentes à ação tóxica das sementes de R. communis, mas que o contrário não ocorre, isto é, bovinos imunizados contra a ação tóxica das sementes de R. communis, não se mostraram protegidos contra a intoxicação por A. precatorius. Estudos anteriores por outros autores mostraram que as toxalbuminas de A. precatorius e R. communis, respectivamente abrina e ricina, são diferentes do ponto de vista antigênico. Uma explicação para a divergência desses resultados com os nossos poderia estar no fato de que no presente estudo foram usados poligástricos que receberam as sementes por via oral, enquanto que nos estudos anteriores foram usados monogástricos em que as sementes ou as toxinas foram aplicadas por via parenteral. A administração de folhas frescas ou do pericarpo do fruto de R. communis a bovinos imunizados contra a ação das sementes desta planta tiveram o mesmo efeito tóxico que em animais não imunizados, demonstrando que a imunidade conferida pela ricina não inibe a ação da ricinina, o principio tóxico das folhas e do pericarpo.

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Characterization of the exposed carbohydrates on the surface membrane of adult Schistosoma mansoni by analysis of lectin binding

Parasitology ◽

10.1017/s0031182000054986 ◽

1980 ◽

Vol 81 (1) ◽

pp. 1-15 ◽

Cited By ~ 50

Author(s):

A. J. G. Simpson ◽

S. R. Smithers

Keyword(s):

Schistosoma Mansoni ◽

Adult Male ◽

Wheat Germ ◽

Ricinus Communis ◽

Specific Binding ◽

Lectin Binding ◽

Surface Membrane ◽

Soybean Agglutinin ◽

Peanut Agglutinin

SUMMARYThe surface architecture of adult male Schistosoma mansoni was explored using a range of lectins with differing carbohydrate specificities. Highest specific binding was achieved with concanavalin A and the agglutinin of molecular weight 60000 from Ricinus communis; the binding of wheat germ agglutinin was mostly non-specific. Small amounts of peanut agglutinin and soybean agglutinin binding were observed and the binding of these lectins was increased by pre-treating the parasite with neuraminidase. The fucose binding protein of Lotus tetragonolobus failed to bind. These results indicate that mannose and/or glucose, galactose, N-acetyl-glucosamine, N-acetyl-galactosamine and sialic acid are exposed on the surface of the adult male schistosome.

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