scholarly journals Changes in protein patterns of Staphylococcus aureus and Escherichia coli by silver nanoparticles capped with poly (4-styrenesulfonic acid-co-maleic acid) polymer

2019 ◽  
Vol 13 (2) ◽  
pp. 39-47 ◽  
Author(s):  
Hathaichanok Tamiyakul ◽  
Sittiruk Roytrakul ◽  
Janthima Jaresitthikunchai ◽  
Narumon Phaonakrop ◽  
Somboon Tanasupawat ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Maleic Acid ◽  
Molecular Transport ◽  
Protein Translation ◽  
Bacterial Protein ◽  
Protein Patterns ◽  
E Coli ◽  
Sds Page

Abstract Background While silver nanoparticles (AgNPs) are increasingly attractive as an antibacterial agent in many applications, the effect of AgNPs on bacterial protein profiles, especially AgNPs stabilized by polymeric molecules, is not well understood. Objectives To investigate the changes in bacterial protein patterns by AgNPs capped with poly (4-styrenesulfonic acid-co-maleic acid) (AgNPs-PSSMA) polymer toward Staphylococcus aureus ATCC 25923 and Escherichia coli ATCC 25922. Methods The growth of bacteria after incubated with AgNPs-PSSMA for different time intervals was determined by optical density at 600 nm. Their protein patterns were observed using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and the proteomic analysis of extracted proteins was determined by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Results AgNPs-PSSMA was able to inhibit the growth of both S. aureus and E. coli cells. The treated bacterial cells expressed more proteins than the untreated cells as seen from SDS-PAGE study. Nanosilver (NS) caused the upregulation of metabolic gene, waaA, in S. aureus cells. For E. coli cells, the upregulated proteins were metabolic genes (srlB, fliE, murD) and other genes dealt with DNA replication (dinG), DNA–RNA transcription (yrdD), RNA– protein translation (rplD), molecular transport (sapF), and signal transduction (tdcF). Conclusions The antibacterial effect of AgNPs-PSSMA may arise by changing the bacterial proteins and thus interfering with the normal cell function.

scholarly journals EVALUACIÓN BIOQUÍMICA Y BACTERICIDA DEL VENENO DE CROTALUS DURISSUS (DEPARTAMENTO DEL TOLIMA)

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Walter Murillo Arango
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
E Coli ◽  
Sds Page ◽  
Crotalus Durissus

En el departamento del Tolima no hay estudios que permitan precisar con certeza la magnitud del accidente ofídico causado por Crotalus durissus; Por lo anterior existe la necesidad de generar información toxicológica, así como conocimiento del posible potencial antimicrobiano de moléculas como proteínas y péptidos que componen el veneno. En este trabajo se analizó el perfil electroforético por SDS-PAGE del veneno crudo extraído de individuos colectados en el municipio de Natagaima (Tolima) y la asociación de sus componentes con la actividad hemolítica directa e indirecta sobre agar sangre y su actividad bactericida. Se evaluó el potencial del veneno crudo contra Escherichia coli, Staphylococcus aureus y Pseudomonas aeuroginosa. El veneno mostró bandas de peso molecular 26.6 kDa., 17, 14.2, 6.5, 3.5 y 1.06 kDa., correspondientes para reportes del veneno de esta especie. La actividad hemolítica directa e indirecta fueron dosis dependiente, siendo la dosis hemolítica mínima de 200 mg/ml. El efecto bactericida del veneno crudo fue diferencial sobre los microorganismos evaluados, presentando actividad moderada sobre E. coli. Los resultados constituyen datos valiosos para reconocer preliminarmente el potencial antimicrobiano del veneno de Crotalus durissus (cascabel) de la zona de Natagaima-Tolima.

scholarly journals Antibacterial Properties of Nanosilver PLLA Fibrous Membranes

2009 ◽  
Vol 2009 ◽  
pp. 1-5 ◽  
Author(s):  
Lin Li ◽  
Yi Li ◽  
Jiashen Li ◽  
Lei Yao ◽  
Arthur F. T. Mak ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Weight Ratio ◽  
Antibacterial Effects ◽  
E Coli ◽  
Fibrous Membranes ◽  
Composite Fibrous Membranes

Nanosilver has been studied as a valuable material for it strong antibacterial effects. In this study, we investigated the antibacterial properties of nano silver Poly-L-Lactic acid (Ag/PLLA) composite fibrous membranes. Ag/PLLA fibrous membranes were prepared with silver nanoparticles having weight ratio of silver nanoparticles to PLLA at 5% (w/w). In vitro antibacterial tests were performed usingEscherichia coli(E. coli) andStaphylococcus aureus(Staph.) to determine the antibacterial capability of the Ag/PLLA fibrous membranes. As the results suggested, Ag/PLLA fibrous membranes showed strong antibacterial properties. Thus, Ag/PLLA fibrous membrane can be used as an antibacterial scaffold for tissue engineering.

scholarly journals Size-Controlled Facile Synthesis of Silver Nanoparticles by Chemical Reduction Method and Their Antibacterial Performance Against Staphylococcus Aureus and Escherichia Coli

2021 ◽  
Author(s):  
Sanjoy Halder ◽  
Aninda Nafis Ahmed ◽  
M A Gafur ◽  
Gimyeong Seong ◽  
Muhammad Zamir Hossain
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Chemical Reduction ◽  
Facile Synthesis ◽  
Capping Agents ◽  
X Ray ◽  
E Coli ◽  
Antibacterial Performance ◽  
Size Controlled

Abstract This article describes the size-controlled one-pot facile synthesis of silver nanoparticles (AgNPs) and their antibacterial response against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) bacteria. AgNPs coated with and without trisodium citrate (TSC) were synthesized using silver nitrate aqueous precursor and hydrazine as a reducing agent. The appearance of yellowish color with surface plasmon resonance (SPR) with maximum absorbance (λmax) at 416 nm from the ultraviolet-visible (UV–vis) spectrum of the product indicated primarily the formation of the desired product. X-ray diffraction (XRD) pattern confirmed the crystallinity of those products. Field emission scanning microscopy (FESEM) and transmission electron microscopy (TEM) images indicated only spherical AgNPs formation. The existence of a functional group of chemically bonded surface capping agents is confirmed by Fourier transform infrared (FTIR) and the themogravimetric analysis (TGA) weight loss assessment proved the attachment of good amount of capping agents. Energy dispersive X-ray (EDX) spectroscopic analysis of products revealed the existence of high percentage (96.36%) of silver. Exposing the synthesized AgNPs to Gram-positive S. aureus and Gram-negative E. coli bacteria, the zone inhibition was found to be 8 mm and 6 mm at a concentration of 50 mgL− 1, respectively. These results imply that TSC capped AgNPs can be considered as effective human pathogens for S. aureus and E. coli which is very inspiring.

Evaluation of Antibacterial Properties of Leather Treated with Silver Nanoparticles

2013 ◽  
Vol 60 (1) ◽  
Author(s):  
Zohreh Majidnia ◽  
Ani Idris ◽  
Peiman Valipour
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Silver Nanoparticles ◽  
Antibacterial Properties ◽  
Test Methods ◽  
Antibacterial Effects ◽  
E Coli ◽  
Leather Processing ◽  
Processing Step ◽  
Unpleasant Smell

Objektif kajian ini adalah untuk merawat kulit dengan nanopartikel perak untuk membangunkan ciri-ciri antibakteria di kulit. Aktiviti biologi ke atas kasut kulit menyebabkan pertumbuhan bakteria dan menyebabkan bau yang tidak menyenangkan. Nanopartikel perak telah diketahui mempunyai beberapa kesan antibakteria dan telah ditambah kepada minyak yang digunakan dalam langkah pemprosesan kulit. Sedikit nanopartikel perak (6 dan 8%v/v) telah dimasukkan ke dalam minyak dan ciri-ciri antibakteria telah dinilai menggunakan rendaman dan kaedah ujian kapasiti. Larutan bakteria, bersamaan kekeruhan McFarland piawai sebanyak 0.5 telah disediakan untuk staphylococcus aureus dan bakteria Escherichia coli. Hasil kajian menunjukkan bahawa ujian antimikrobial ke atas sampel yang mengandungi nanopartikel perak menunjukkan ciri-ciri antibakteria yang indikasi oleh Piawai Antarabangsa (ISO 22196) kelembutan penggantungan kurang dari 3x108 CFU/ml. Oleh itu kulit yang dirawat dengan nanopartikel perak mempunyai ciri-ciri antibakteria dengan kecenderungan untuk mengurangkan bau-bau tidak menyenangkan. Kata kunci: Kulit; sifat anti-bakteria; nanopartikel; perak The objective of this study is to treat leather with silver nanoparticles so as to develop antibacterial properties in the leather. The biological activity on leather shoes resulted in bacterial growth and caused the unpleasant smell. Silver nanoparticles have been known to possess some antibacterial effects and were added to the oil used in the leather processing step. A small amount of silver nanoparticles (6 and 8%v/v) were added to oil and its antibacterial properties have been evaluated using the immersion and capacity test methods. The bacteria suspension, a turbidity equivalent to a 0.5 McFarland standard was prepared for the S. aureus and E. coli bacteria. Results revealed that antimicrobial tests on samples containing silver nanoparticles showed suitable antibacterial properties indicated by the International Standard (ISO 22196) tenderness of suspension of less than 3x108 CFU/ml. Thus leather treated with silver nanoparticles has antibacterial properties with the tendency to reduce the unpleasant odors. Keywords: Leather; antibacterial properties; nanoparticle, silver

EFECTO INHIBITORIO DE EXTRACTOS DE Vitex mollis Kunth CONTRA BACTERIAS Y ESPECIES DE Fusarium DE IMPORTANCIA HUMANA Y AGRÍCOLA

2018 ◽  
Vol 41 (4) ◽  
pp. 353-363
Author(s):  
Alberto J. Valencia-Botin ◽  
Melesio Gutiérrez-Lomelí ◽  
Juan A. Morales-Del-Río ◽  
Pedro J. Guerrero-Medina ◽  
Miguel A. Robles-García ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Enterica ◽  
Micrococcus Luteus ◽  
E Coli

Actualmente existe la necesidad de hacer frente al problema de la resistencia a los antibióticos y al uso indiscriminado de fungicidas químicos en la agricultura. El objetivo de este trabajo fue evaluar el efecto inhibitorio de extractos acuosos, metanólicos, acetónicos y hexánicos de hoja y tallo de Vitex mollis Kunth (Lamiaceae) contra diferentes bacterias (Escherichia coli, Micrococcus luteus, Salmonella enterica y Staphylococcus aureus) y especies del hongo Fusarium (F. verticillioides, F. oxysporum, F. tapsinum y F. oxysporum f.sp. lycopersici) de importancia en la salud y en la agricultura, así como determinar su composición química general. Se determinaron las concentraciones inhibitorias mínimas (CIM) de todos los extractos por la técnica de microdilución, excepto del hexánico, que no presentó inhibición en las bacterias estudiadas. S. enterica fue la bacteria que mostró mayor sensibilidad al extracto metanólico de tallo (CIM = 28 μg mL-1), le siguieron M. luteus (CIM = 32 μg mL-1), S. aureus (CIM = 75 μg mL-1) y E. coli (CIM = 80 μg mL- 1). Los extractos metanólicos y acuosos de tallo presentaron mayor porcentaje de inhibición contra los diferentes tipos de Fusarium evaluados por el método de dilución en agar. Los extractos de V. mollis inhibieron a F. verticillioides entre 62 y 91 % con 120 μg mL-1 de extracto. El orden de las especies de hongos inhibidas por los extractos fue: F. verticillioides > F. oxysporum > F. tapsinum > F. oxysporum f.sp. lycopersici. La composición química de las especies se determinó mediante pruebas para fenoles, taninos, flavonoides, triterpenos, alcaloides, cumarinas y saponinas. Ninguno de los extractos presentó alcaloides y saponinas. Los fenoles (37.1 mg EAG/g muestra seca) y flavonoides (26.8 mg EQ/g muestra seca) fueron los compuestos mayoritarios en los extractos metanólicos y acuosos. En conclusión, se requieren cantidades muy pequeñas de extracto para la inhibición de bacterias y de Fusarium; por lo tanto, V. mollis puede ser considerada una fuente de metabolitos para este fin y en la agricultura como control alternativo dentro de un manejo integrado de enfermedades.

Antimicrobial Activity of Silver Nanoparticles Prepared Under an Ultrasonic Field

2011 ◽  
Vol 4 (3) ◽  
pp. 1491-1496
Author(s):  
Umadevi M ◽  
Rani T ◽  
Balakrishnan T ◽  
Ramanibai R
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Reduction Method ◽  
Therapeutic Potential ◽  
Chemical Reduction ◽  
Ultrasonic Field ◽  
Great Promise ◽  
Chemical Reduction Method ◽  
E Coli

Nanotechnology has great promise for improving the therapeutic potential of medicinal molecules and related agents. In this study, silver nanoparticles of different sizes were synthesized in an ultrasonic field using the chemical reduction method with sodium borohydride as a reducing agent. The size effect of silver nanoparticles on antimicrobial activity were tested against the microorganisms Staphylococcus aureus (MTCC No. 96), Bacillus subtilis (MTCC No. 441), Streptococcus mutans (MTCC No. 497), Escherichia coli (MTCC No. 739) and Pseudomonas aeruginosa (MTCC No. 1934). The results shows that B. subtilis, and E. coli were more sensitive to silver nanoparticles and its size, indicating the superior antimicrobial efficacy of silver nanoparticles. 

Substituted 4-Formyl-2H-chromen-2-ones: Their Reaction with N-(2,3,4,6-Tetra-Oacetyl- β-D-galactopyranosyl)thiosemicarbazide, Antibacterial and Antifungal Activity of Their Thiosemicarbazone Products

2020 ◽  
Vol 24 (19) ◽  
pp. 2272-2282
Author(s):  
Vu Ngoc Toan ◽  
Nguyen Minh Tri ◽  
Nguyen Dinh Thanh
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Candida Albicans ◽  
Selenium Dioxide ◽  
Antibacterial And Antifungal Activity ◽  
E Coli ◽  
Antibacterial And Antifungal Activities ◽  
Alkyl Ethers ◽  
Antibacterial And Antifungal

Several 6- and 7-alkoxy-2-oxo-2H-chromene-4-carbaldehydes were prepared from corresponding alkyl ethers of 6- and 7-hydroxy-4-methyl-2-oxo-2H-chromen-2-ones by oxidation using selenium dioxide. 6- and 7-Alkoxy-4-methyl-2H-chromenes were obtained with yields of 57-85%. Corresponding 4-carbaldehyde derivatives were prepared with yields of 41-67%. Thiosemicarbazones of these aldehydes with D-galactose moiety were synthesized by reaction of these aldehydes with N-(2,3,4,6-tetra-O-acetyl-β-Dgalactopyranosyl) thiosemicarbazide with yields of 62-74%. These thiosemicarbazones were screened for their antibacterial and antifungal activities in vitro against bacteria, such as Staphylococcus aureus, Escherichia coli, and fungi, such as Aspergillus niger, Candida albicans. Several compounds exhibited strong inhibitory activity with MIC values of 0.78- 1.56 μM, including 8a (against S. aureus, E. coli, and C. albicans), 8d (against E. coli and A. niger), 9a (against S. aureus), and 9c (against S. aureus and C. albicans).

Synthesis of Chalcones and Nucleosides Incorporating [1, 3, 4]Oxadiazolenone Core and Evaluation of their Antifungal and Antibacterial Activities

2020 ◽  
Vol 15 (6) ◽  
pp. 665-679
Author(s):  
Alok K. Srivastava ◽  
Lokesh K. Pandey
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacillus Subtilis ◽  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Good Antibacterial Activity

Background: [1, 3, 4]oxadiazolenone core containing chalcones and nucleosides were synthesized by Claisen-Schmidt condensation of a variety of benzaldehyde derivatives, obtained from oxidation of substituted 5-(3/6 substituted-4-Methylphenyl)-1, 3, 4-oxadiazole-2(3H)-one and various substituted acetophenone. The resultant chalcones were coupled with penta-O-acetylglucopyranose followed by deacetylation to get [1, 3, 4] oxadiazolenone core containing chalcones and nucleosides. Various analytical techniques viz IR, NMR, LC-MS and elemental analysis were used to confirm the structure of the synthesised compounds.The compounds were targeted against Bacillus subtilis, Staphylococcus aureus and Escherichia coli for antibacterial activity and Aspergillus flavus, Aspergillus niger and Fusarium oxysporum for antifungal activity. Methods: A mixture of Acid hydrazides (3.0 mmol) and N, Nʹ- carbonyl diimidazole (3.3 mmol) in 15 mL of dioxane was refluxed to afford substituted [1, 3, 4]-oxadiazole-2(3H)-one. The resulted [1, 3, 4]- oxadiazole-2(3H)-one (1.42 mmol) was oxidized with Chromyl chloride (1.5 mL) in 20 mL of carbon tetra chloride and condensed with acetophenones (1.42 mmol) to get chalcones 4. The equimolar ratio of obtained chalcones 4 and β -D-1,2,3,4,6- penta-O-acetylglucopyranose in presence of iodine was refluxed to get nucleosides 5. The [1, 3, 4] oxadiazolenone core containing chalcones 4 and nucleosides 5 were tested to determined minimum inhibitory concentration (MIC) value with the experimental procedure of Benson using disc-diffusion method. All compounds were tested at concentration of 5 mg/mL, 2.5 mg/mL, 1.25 mg/mL, 0.62 mg/mL, 0.31 mg/mL and 0.15 mg/mL for antifungal activity against three strains of pathogenic fungi Aspergillus flavus (A. flavus), Aspergillus niger (A. niger) and Fusarium oxysporum (F. oxysporum) and for antibacterial activity against Gram-negative bacterium: Escherichia coli (E. coli), and two Gram-positive bacteria: Staphylococcus aureus (S. aureus) and Bacillus subtilis(B. subtilis). Result: The chalcones 4 and nucleosides 5 were screened for antibacterial activity against E. coli, S. aureus and B. subtilis whereas antifungal activity against A. flavus, A. niger and F. oxysporum. Compounds 4a-t showed good antibacterial activity whereas compounds 5a-t containing glucose moiety showed better activity against fungi. The glucose moiety of compounds 5 helps to enter into the cell wall of fungi and control the cell growth. Conclusion: Chalcones 4 and nucleosides 5 incorporating [1, 3, 4] oxadiazolenone core were synthesized and characterized by various spectral techniques and elemental analysis. These compounds were evaluated for their antifungal activity against three fungi; viz. A. flavus, A. niger and F. oxysporum. In addition to this, synthesized compounds were evaluated for their antibacterial activity against gram negative bacteria E. Coli and gram positive bacteria S. aureus, B. subtilis. Compounds 4a-t showed good antibacterial activity whereas 5a-t showed better activity against fungi.

Comparison of the Effects of Silver in Nanostructured and Ultrahigh Diluted Form on Growth and Volatile Compounds Produced by Escherichia coli and Staphylococcus aureus

2020 ◽  
Vol 10 (3) ◽  
pp. 316-329
Author(s):  
Fateme Mirzajani ◽  
Amin Hamidi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Life Cycles ◽  
Gas Chromatography Mass Spectrometry ◽  
Untreated Sample ◽  
E Coli ◽  
Volatile Metabolites ◽  
Octyl Acetate ◽  
Volatile Organic ◽  
And Staphylococcus Aureus

Introduction: In this project, the growth and volatile metabolites profiles of Escherichia coli (E. coli ) and Staphylococcus aureus were monitored under the influence of silver base chemical, nanoparticle and ultra-highly diluted compounds. Materials & Methods: The treatments were done for 12000 life cycles using silver nanoparticles (AgNPs) as well as ultra-highly diluted Argentum nitricum (Arg-n). Volatile organic metabolites analysis was performed using gas chromatography mass spectrometry (GC-MS). The results indicated that AgNPs treatment made the bacteria resistant and adapted to growth in the nanoparticle condition. The use of ultra-highly diluted Arg-n initially increased growth but it decreased later. Also, with the continuous usage of these materials, no more bacterial growth was observed. Results: The most important compounds produced by E. coli are Acetophenone, Octyl acetate, Styrene, 1,8-cineole, 4-t-butyl-2-(1-methyl-2-nitroethyl)cyclohexane, hexadecane and 2-Undecanol. The main compounds derived from S. aureus are Acetophenone,1,8-cineole, Benzaldehyde, 2-Hexan-1-ol, Tridecanol, Dimethyl Octenal and tetradecane. Acetophenone and 1,8-cineole were common and produced by both organisms. Conclusion: Based on the origin of the produced volatiles, main volatiles percentage of untreated sample is hydrocarbon (>50%), while bacteria treatments convert the ratio in to aldehydes, ketones and alcohols in the case of AgNPs, (>80%) and aldehydes, ketones and terpenes in the case of Arg-n (>70%).

TiO2 antifouling coating based on epoxy-modified tung oil waterborne resin

2021 ◽  
pp. 096739112110111
Author(s):  
Hailiang Hu ◽  
Minmin Chen ◽  
Mengye Cao
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Survival Rate ◽  
Light Irradiation ◽  
Fouling Resistance ◽  
Tung Oil ◽  
E Coli ◽  
Antifouling Coating ◽  
Waterborne Epoxy

The eco-friendly functionalized TiO2/polymer antifouling (AF) coating was successfully synthesized by dispersing TiO2 nanoparticles in waterborne epoxy-modified tung oil resin. The AF effectiveness of coating was evaluated toward Staphylococcus aureus ( S. aureus, ATCC6538), Escherichia coli ( E. coli, ATCC8739) and diatom ( Cyclotella sp., FACHB-1635). The nanoTiO2/polymer AF coating showed good antimicrobial activity both under the light and dark conditions by comparison with the pristine TiO2 nanoparticles and bulk polymer. Under light irradiation for 50 min, the AF coating showed only 8.4% and 8% survival rate for S. aureus and E. coli. In addition, The AF coatings exhibited favorable inhibition efficacy toward the growth and adhesion of Cyclotella sp., and the efficacy was enhanced with the increase of TiO2 content. It can be concluded that TiO2 nanoparticles endow the AF coatings with promoted fouling resistance properties.

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