Retinol Does not Affect Cell Growth in Fibroblast Cell Cultures

The effect of adenosine on proliferation of human endothelial cells was investigated by adding adenosine to the medium of cultures derived from human umbilical veins. Cell counts on cultures grown in 10 microM adenosine for 4–7 days were 41–53% greater than counts from control cultures. In contrast, 10 microM adenosine had no effect on growth of a human fibroblast cell strain (IMR-90). Neither inosine nor 2',5'-dideoxyadenosine influenced endothelial cell growth at concentrations of 0.1 or 10 microM. Addition of adenosine deaminase abolished the proliferative effect of added adenosine and inhibited proliferation by 16% in control cultures, suggesting that endogenous adenosine may enhance proliferation in culture. The adenosine receptor antagonist, 8-phenyltheophylline, at 0.1 and 1.0 microM blocked the enhanced proliferation caused by 10 microM adenosine. Addition of 10 microM adenosine enhanced DNA synthesis in endothelial cell cultures as indicated by an increased incorporation of [3H]thymidine into acid-insoluble cell material. The results indicate that addition of physiological concentrations of adenosine to human umbilical vein endothelial cell cultures stimulates proliferation, possibly via a surface receptor, and suggest that adenosine may be a factor for human endothelial cell growth and possibly angiogenesis.

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The molecular weight and concentration of dextran sulfate affect cell growth and antibody production in CHO cell cultures

Biotechnology Progress ◽

10.1002/btpr.2287 ◽

2016 ◽

Vol 32 (5) ◽

pp. 1113-1122 ◽

Cited By ~ 15

Author(s):

Jin Hyoung Park ◽

Myung Sin Lim ◽

Ju Rang Woo ◽

Jong Won Kim ◽

Gyun Min Lee

Keyword(s):

Molecular Weight ◽

Cell Growth ◽

Cell Cultures ◽

Antibody Production ◽

Dextran Sulfate ◽

Cho Cell ◽

Affect Cell Growth

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Rotary Beveling for In Situ Thin-Section Microscopy of Cell Cultures

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100099192 ◽

1981 ◽

Vol 39 ◽

pp. 550-551

Author(s):

Dean A. Handley ◽

Jack T. Alexander ◽

Shu Chien

Keyword(s):

Cell Growth ◽

Cell Cultures ◽

Molecular Interactions ◽

Convenient Method ◽

Petri Dish ◽

Simple Method ◽

Thin Section Microscopy ◽

Thin Sectioning ◽

Organic Fluids

In situ preparation of cell cultures for ultrastructural investigations is a convenient method by which fixation, dehydration and embedment are carried out in the culture petri dish. The in situ method offers the advantage of preserving the native orientation of cell-cell interactions, junctional regions and overlapping configurations. In order to section after embedment, the petri dish is usually separated from the polymerized resin by either differential cryo-contraction or solvation in organic fluids. The remaining resin block must be re-embedded before sectioning. Although removal of the petri dish may not disrupt the native cellular geometry, it does sacrifice what is now recognized as an important characteristic of cell growth: cell-substratum molecular interactions. To preserve the topographic cell-substratum relationship, we developed a simple method of tapered rotary beveling to reduce the petri dish thickness to a dimension suitable for direct thin sectioning.

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Survival and Function of Fibroblasts Stored as Stock Cultures at a Non-freezing Temperature

Alternatives to Laboratory Animals ◽

10.1177/026119299302100215 ◽

1993 ◽

Vol 21 (2) ◽

pp. 206-209

Author(s):

Anders H. G. Andrén ◽

Anders P. Wieslander

Keyword(s):

Cell Growth ◽

Cell Line ◽

Cell Lines ◽

Fibroblast Cell ◽

Freezing Temperature ◽

Mouse Fibroblast ◽

Toxic Response ◽

Normal Manner ◽

And Function

Cytotoxicity, measured as inhibition of cell growth of cultured cell lines, is a widely used method for testing the safety of biomaterials and chemicals. One major technical disadvantage with this method is the continuous routine maintenance of the cell lines. We decided to investigate the possibility of storing stock cultures of fibroblasts (L-929) in an ordinary refrigerator as a means of reducing the routine workload. Stock cultures of the mouse fibroblast cell line L-929 were prepared in plastic vials with Eagle's minimum essential medium. The vials were stored in a refrigerator at 4–10°C for periods of 7–31 days. The condition of the cells after storage was determined as cell viability, cell growth and the toxic response to acrylamide, measured as cell growth inhibition. We found that the L-929 cell line can be stored for 2–3, weeks with a viabilty > 90% and a cell growth of about 95%, compared to L-929 cells grown and subcultured in the normal manner. The results also show that the toxic response to acrylamide, using refrigerator stored L-929 cells, corresponds to that of control L-929 cells. We concluded that it is possible to store L-929 cells in a refrigerator for periods of up to 3 weeks and still use the cells for in vitro cytotoxic assays.

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Propeptide-mediated regulation of procollagen synthesis in IMR-90 human lung fibroblast cell cultures. Evidence for transcriptional control.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(18)49944-9 ◽

1991 ◽

Vol 266 (5) ◽

pp. 2983-2987 ◽

Cited By ~ 5

Author(s):

C H Wu ◽

C M Walton ◽

G Y Wu

Keyword(s):

Cell Cultures ◽

Transcriptional Control ◽

Human Lung ◽

Fibroblast Cell ◽

Lung Fibroblast ◽

Human Lung Fibroblast

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Rapid Detection of Herpes Simplex Virus Using a Combination of Human Fibroblast Cell Cultures and Peroxidase-Antiperoxidase Staining

American Journal of Clinical Pathology ◽

10.1093/ajcp/81.1.43 ◽

1984 ◽

Vol 81 (1) ◽

pp. 43-47 ◽

Cited By ~ 7

Author(s):

Michael P. Meyer ◽

Antonio J. Amortegui

Keyword(s):

Herpes Simplex Virus ◽

Herpes Simplex ◽

Cell Cultures ◽

Human Fibroblast ◽

Rapid Detection ◽

Fibroblast Cell ◽

Human Fibroblast Cell ◽

Simplex Virus

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Prohibitins Regulate Membrane Protein Degradation by the m-AAA Protease in Mitochondria

Molecular and Cellular Biology ◽

10.1128/mcb.19.5.3435 ◽

1999 ◽

Vol 19 (5) ◽

pp. 3435-3442 ◽

Cited By ~ 208

Author(s):

Gregor Steglich ◽

Walter Neupert ◽

Thomas Langer

Keyword(s):

Saccharomyces Cerevisiae ◽

Cell Growth ◽

Membrane Proteins ◽

Eukaryotic Cells ◽

Mitochondrial Morphology ◽

Regulatory Effect ◽

Inner Membrane ◽

Functional Activities ◽

Native Molecular Mass ◽

Affect Cell Growth

ABSTRACT Prohibitins comprise a protein family in eukaryotic cells with potential roles in senescence and tumor suppression. Phb1p and Phb2p, members of the prohibitin family in Saccharomyces cerevisiae, have been implicated in the regulation of the replicative life span of the cells and in the maintenance of mitochondrial morphology. The functional activities of these proteins, however, have not been elucidated. We demonstrate here that prohibitins regulate the turnover of membrane proteins by the m-AAA protease, a conserved ATP-dependent protease in the inner membrane of mitochondria. The m-AAA protease is composed of the homologous subunits Yta10p (Afg3p) and Yta12p (Rca1p). Deletion ofPHB1 or PHB2 impairs growth of Δyta10 or Δyta12 cells but does not affect cell growth in the presence of the m-AAA protease. A prohibitin complex with a native molecular mass of approximately 2 MDa containing Phb1p and Phb2p forms a supercomplex with them-AAA protease. Proteolysis of nonassembled inner membrane proteins by the m-AAA protease is accelerated in mitochondria lacking Phb1p or Phb2p, indicating a negative regulatory effect of prohibitins on m-AAA protease activity. These results functionally link members of two conserved protein families in eukaryotes to the degradation of membrane proteins in mitochondria.

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