Stability of routine biochemical analytes in whole blood and plasma/serum: focus on potassium stability from lithium heparin

2018 ◽  
Vol 56 (3) ◽  
pp. 413-421 ◽  
Author(s):  
Anne Marie Dupuy ◽  
Jean Paul Cristol ◽  
Bruno Vincent ◽  
Anne Sophie Bargnoux ◽  
Mickael Mendes ◽  
...  
Keyword(s):  
Whole Blood ◽  
Venous Blood ◽  
Stability Study ◽  
Serum Samples ◽  
Total Change ◽  
Courier Service ◽  
Blood Specimens ◽  
The Stability ◽  
Clinical Departments ◽  
And Storage

AbstractBackground:Blood specimens are transported from clinical departments to the biochemistry laboratory by hospital courier service, sometimes over long distances. The aim of this study was to assess the stability of common biochemical analytes in venous blood under our routine transport conditions and to evaluate analyte stability after prompt or delayed centrifugation.Methods:We investigated pre- and postanalytical contributions of 32 biochemical analytes in plasma and serum samples from 10 patients (healthy adults and patients from intensive care units). Differences in analyte concentrations between baseline (T0) and different time intervals (2, 4, 6, 8, 12 and 24 h) following storage after prompt and delayed centrifugation were reported. Evaluation was against the total change limit as described by Oddoze et al. (Oddoze C, Lombard E, Portugal H. Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin Biochem 2012;45:464–9).Results:The majority of analytes were stable with delayed separation up to 12 h, except for potassium, C-peptide, osteocalcin, parathyroid hormone (PTH), bicarbonate and LDH. After prompt centrifugation and storage at 4°C, stability was greatly increased up to 48 h for most analytes. LDH and bicarbonate had the lowest stability after centrifugation; therefore, no reanalysis of these analytes in a centrifuged tube can be allowed.Conclusions:Knowledge of analyte stability is crucial to interpret biological analysis with confidence. However, centrifugation prior to transport is time consuming, and the transfer of plasma or serum from a primary tube to a secondary tube increases the risk of preanalytical errors. For analytes that are stable in whole blood for 24 h or more, it seems that there is no benefit to centrifuge before transport.

Stability of 5-fluorouracil in whole blood and plasma.

1987 ◽  
Vol 33 (12) ◽  
pp. 2299-2300 ◽  
Author(s):  
R F Murphy ◽  
F M Balis ◽  
D G Poplack
Keyword(s):  
Whole Blood ◽  
Enzymatic Degradation ◽  
Room Temperature ◽  
Parent Drug ◽  
Plasma Samples ◽  
Blood Specimens ◽  
The Stability ◽  
Frozen Plasma

Abstract We studied the stability of 5-fluorouracil (5-FU) in plasma and whole blood kept at room temperature and on ice for 1 to 24 h. At room temperature, there was a steady loss of 94% of the parent drug over 24 h in whole blood and 52% in plasma. In the presence of an excess of uracil, 5-FU was stable for 24 h, suggesting that the loss of 5-FU is the result of enzymatic degradation. 5-FU is more stable in whole blood and plasma when samples are kept cold. For blood and plasma samples maintained on ice, the loss was only 30% and 10% of the parent drug in the respective samples over 24 h. Frozen plasma samples (-20 degrees C) were stable for five weeks. Blood specimens collected for quantifying 5-FU should be immediately placed on ice, and the plasma should be separated and frozen as promptly as possible.

Short-term stability of free metanephrines in plasma and whole blood

2020 ◽  
Vol 58 (5) ◽  
pp. 753-757 ◽  
Author(s):  
Elisa Danese ◽  
Martina Montagnana ◽  
Claudio Brentegani ◽  
Giuseppe Lippi
Keyword(s):  
Whole Blood ◽  
Repeated Measures ◽  
Sample Collection ◽  
Short Term ◽  
Total Change ◽  
Term Stability ◽  
Before And After ◽  
Liquid Chromatography Tandem Mass ◽  
The Mean ◽  
The Stability

AbstractBackgroundAnalysis of plasma metanephrine (MN) and normetanephrine (NMN) with liquid chromatography tandem mass spectrometry (LC-MS/MS) is the gold standard for the screening of pheochromocytomas and paragangliomas (PPGLs). As scarce information is available on the stability of MNs in diagnostic samples, this study was aimed at analyzing the short-term stability of plasma free MNs in whole blood and plasma, using LC-MS/MS.MethodsThe stability of plasma MNs was evaluated after sample collection at 1, 2 and 3 h in whole blood, and at 2, 4 and 6 h in centrifuged samples. Both studies were performed while maintaining the samples at room temperature (RT) and at 4 °C. The ClinMass Complete Kit (Recipe, Munchen, Germany) was used for measuring MNs with LC-MS/MS (Nexera X2 UHPLC-4500MD Sciex). Differences from the baseline (T0) were assessed using repeated measures one-way ANOVA, Students’ paired t-test and a comparison of the mean percentage changes with the total change limit (TCL).ResultsStatistically significant differences from T0 were found for both MNs (p < 0.001) in whole blood stored at RT, and for NMN (p = 0.028) but not MN (p = 0.220) at 4 °C. The mean difference exceeded the TCL after 1 h and 3 h at RT for MN, and after 1 h at RT for NMN. Statistically significant differences from T0 were only observed in the plasma samples for NMN at RT (p = 0.012), but the variation was within the TCL.ConclusionsMN and NMN displayed different patterns of stability before and after centrifugation. Even short-time storage at RT in whole blood should hence be avoided.

Stability of Blood Biochemistry Levels in Animal Model Research: Effects of Storage Condition and Time

2009 ◽  
Vol 11 (4) ◽  
pp. 395-400 ◽  
Author(s):  
Tai-Chu Peng ◽  
Bang-Gee Hsu ◽  
Fwu-Lin Yang ◽  
Yann Fen C. Chao ◽  
Horng-Jyh Harn ◽  
...  
Keyword(s):  
Whole Blood ◽  
Intraclass Correlation ◽  
Lactate Concentration ◽  
Storage Condition ◽  
Measurement Variation ◽  
Model Research ◽  
Two Samples ◽  
Wistar Kyoto ◽  
Blood Specimens ◽  
The Stability

The purpose of this study was to compare whole blood and plasma in terms of the subsequent accuracy of blood lactate, glucose, lactate dehydrogenase (LDH), creatine phosphokinase (CPK), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) measurement. Blood samples were drawn from 8 male Wistar-Kyoto rats. The rats were homologous, weighed 300— 380 g, were housed in the same environment, and were provided with food and water under the same conditions. Blood draws occurred in all rats at same time. The blood specimens were divided into two samples, one to be stored as whole blood (WBS) and one to be stored as plasma (PS). All the blood sample analyses were performed by trained and experienced personnel to ensure that differences in results were due to variation in form in which specimens were stored rather than to technique. The lactate concentration in the WBS group gradually increased over time, intraclass correlation coefficient (ICC) = 0.541, 95% confidence interval (CI; —0.197, 0.893), and was higher than that of the PS group, ICC = 0.897, 95% CI (0.733, 0.976). By contrast, glucose level gradually declined for the WBS group, ICC= —0.367, 95% CI (—2.563, 0.682). Whole blood storage increased measurement variation for lactate, glucose, LDH, and CPK. Plasma storage prolonged the stability of the biochemical components. This study demonstrates the importance of evaluating validity at each stage of developing and testing animal models.

scholarly journals Evaluation of an ELISA for metanephrines in feline urine

2018 ◽  
Vol 30 (6) ◽  
pp. 887-893
Author(s):  
Thanikul Srithunyarat ◽  
Anna Svensson ◽  
Sofia Hanås ◽  
Odd V. Höglund ◽  
Ragnvi Hagman ◽  
...  
Keyword(s):  
Neuroendocrine Tumors ◽  
Home Environment ◽  
Human Urine ◽  
Room Temperature ◽  
Storage Time ◽  
Stability Study ◽  
Urine Samples ◽  
Urine Sampling ◽  
The Stability ◽  
And Storage

Catecholamines can be used to evaluate neuroendocrine tumors, stress, and potentially pain, but catecholamines degrade rapidly. Their metabolites normetanephrine (NME) and metanephrine (ME) have better stability in urine. In cats, urine sampling in a home environment would be beneficial to reduce effects of clinical stress and simplify sampling. We evaluated a human urine ELISA for analysis of NME and ME in feline urine, and investigated the effects of acidification, cat tray pellets, and storage time at room temperature up to 8.5 h. In 26 feline urine samples, mean NME concentration was 192 ± 80 ng/mL, mean intra- and inter-assay CV was 6.5% and 4.2%, respectively, and spike recovery was 98–101%, but dilutional recovery was unsatisfactory. For ME, mean intra- and inter-assay CV was 10.2% and 4.1%, respectively. Mean urine ME concentration was 32.1 ± 18.3 ng/mL, close to the kit’s lowest standard, and spike recovery was 65–90%; the ELISA could not be validated for ME. The stability study, performed for NME on 12 urine samples, did not identify differences between acidified and non-acidified samples, cat tray pellets, or storage time, and no interaction effects. The ME ELISA was not suitable for feline urine; performance of the NME ELISA was acceptable, except for dilution recovery. For analysis of NME, feline urine can be sampled at home using cat tray pellets and stored at room temperature up to 8.5 h without acidification.

An evaluation of the capillus HIV-1/HIV-2 latex agglutination test using serum and whole blood

1997 ◽  
Vol 8 (3) ◽  
pp. 192-195 ◽  
Author(s):  
I. M. Windsor ◽  
M. L. Gomes Dos Santos ◽  
L. I. De La Hunt ◽  
A. A. Wadee ◽  
S. Khumalo ◽  
...  
Keyword(s):  
Health Care ◽  
Primary Health Care ◽  
Whole Blood ◽  
Screening Test ◽  
Latex Agglutination ◽  
Serum Samples ◽  
Primary Health ◽  
Initial Sensitivity ◽  
Blood Specimens ◽  
Hiv 1

Summary: The capillus HIV-1/HIV-2 latex agglutination (LA) test was evaluated for its potential as an initial screening test in primary health care. For the serum study, panels totalling 289 HIV-positive sera and 323 known HIV-negative sera plus 50 individual seroconversion samples were tested by capillus. Paired blood specimens were also collected in heparinized and plain tubes from 501 consecutive patients with newly diagnosed sexually transmitted diseases (STDs) attending an STD clinic at a Transvaal hospital. Overall, an initial sensitivity of 99.3% and an initial specificity of 99.7% were obtained by visual reading of the capillus HIV-1/ HIV-2 LA tests on serum samples. Capillus also detected 40 (80%) of the 50 seroconversion samples. Of the 501 paired plain and heparinized blood specimens, serum testing by enzyme immunoassay (EIA) and indirect immunofluorescence/ Western blot (IFA/WB) showed 147 (29%) to be HIV Ab positive. Capillus testing of the paired specimens correctly identified all 147 known positive patients and 345 of the 346 negative patients, thus showing an initial sensitivity of 100% and an initial specificity of 99.7% for the testing of heparinized whole blood by a relatively unskilled health worker. It was concluded that the capillus HIV-1/HIV-2 LA test would be suitable for use as a primary screening test in small outlying laboratories or primary health care clinics.

scholarly journals Measurement of the alcohol biomarker phosphatidylethanol (PEth) in dried blood spots and venous blood—importance of inhibition of post-sampling formation from ethanol

2021 ◽  
Author(s):  
Olof Beck ◽  
Maria Mellring ◽  
Christian Löwbeer ◽  
Sabina Seferaj ◽  
Anders Helander
Keyword(s):  
Filter Paper ◽  
Whole Blood ◽  
Room Temperature ◽  
Venous Blood ◽  
Dried Blood Spots ◽  
Blood Samples ◽  
Ethanol Formation ◽  
Alcohol Biomarker ◽  
Blood Specimens ◽  
Blood Spot

AbstractPhosphatidylethanol (PEth) is a group of phospholipids formed in cell membranes following alcohol consumption by action of the enzyme phospholipase D (PLD). PEth measurement in whole blood samples is established as a specific alcohol biomarker with clinical and forensic applications. However, in blood specimens containing ethanol, formation of PEth may continue after sampling leading to falsely elevated concentrations. This study evaluated the use of dried blood spot (DBS) and microsampling specimens to avoid post-sampling formation of PEth. Filter paper cards and three commercial devices for volumetric microsampling of finger-pricked blood were assessed, using PEth-negative and PEth-positive whole blood fortified with 2 g/L ethanol. PEth (16:0/18:1) was measured by LC–MS/MS. Post-sampling formation of PEth occurred in wet blood and in the volumetric devices, but not filter paper cards, when stored at room temperature for 48 h. Addition of an inhibitor of PLD, sodium metavanadate (NaVO3), eliminated post-sampling formation during storage and drying. In conclusion, the present study confirmed previous observations that PEth can be formed in blood samples after collection, if the specimen contains ethanol. The results further demonstrated that post-sampling formation of PEth from ethanol also occurred with commercial devices for volumetric dried blood microsampling. In order for a PEth result not to be questioned, it is recommended to use a PLD inhibitor, whether venous blood is collected in a vacutainer tube or finger-pricked blood is obtained using devices for dried blood microsampling. Graphical abstract

scholarly journals Role of biobanking in managing large-scale epidemiological studies

2021 ◽  
Vol 20 (5) ◽  
pp. 2958
Author(s):  
M. S. Pokrovskaya ◽  
A. L. Borisova ◽  
V. A. Metelskaya ◽  
I. A. Efimova ◽  
Yu. V. Doludin ◽  
...  
Keyword(s):  
Blood Serum ◽  
Whole Blood ◽  
Large Scale ◽  
Venous Blood ◽  
Epidemiological Studies ◽  
National Medical ◽  
And Storage ◽  
Large Scale Epidemiological Study

The success and quality of large-scale epidemiological studies depends entirely on biomaterial quality. Therefore, when arranging the third Epidemiology of Cardiovascular Diseases and their Risk Factors in Regions of Russian Federation (ESSE-RF-3) study, increased attention was paid to specifics of collection, processing and further transportation of biological samples and related clinical and anthropometric data of participants from regional collection centers to Biobank.Aim. To develop a methodology for collection of high-quality biomaterials within the large-scale epidemiological study, involving the sampling, processing, freezing of blood and its derivatives (serum, plasma) in the regions, followed by transportation and storage of obtained biomaterial in the Biobank of National Medical Research Center for Therapy and Preventive Medicine (Moscow).Material and methods. To conduct the ESSE-RF-3 study, a design was developed, according to which the collection of venous blood samples in a total volume of 29,5 ml from each participant is planned in all participating regions in order to obtain and store samples of whole blood, serum and two types of plasma.Results. On the basis of international biobanking standards, ethical norms, experience from ESSE-RF and ESSE-RF-2, and literature data, a protocol for biobanking of blood and its derivatives was developed. The type and number of serum and plasma aliquots obtained, the required standard technical means and consumables, as well as logistic biomaterial requirements were determined. Training programs for regional participants were developed. By the beginning of August 2021, 180 thousand samples of whole blood, serum and plasma from more than 23 thousand participants from 28 Russian regions were collected, processed and stored.Conclusion. The presented work made it possible to assess and confirm the compliance of developed biobanking protocol with quality requirements. However, due to the coronavirus disease 2019 pandemic, by August 2021, the Biobank did not reach the maximum effectiveness predicted for the ESSE-RF-3 project.

scholarly journals Development of methods for analysis of the amount of flavonoids and their stability study in the combined dental gel

2021 ◽  
pp. 3-10
Author(s):  
Yu. Maslii ◽  
A. Materiienko ◽  
O. Ruban ◽  
I. Bezruk ◽  
L. Ivanauskas
Keyword(s):  
Stability Study ◽  
Biologically Active Substances ◽  
Biologically Active ◽  
Plant Origin ◽  
Active Components ◽  
Drug Substances ◽  
The Stability ◽  
And Storage ◽  
Active Substances

An important aspect in the pharmaceutical development of dental medicines is to provide them with a prolonged therapeutic effect while reducing the side effects of drug substances and the possibility of long-term use. This can be achieved by using active components of plant origin. Aim. To develop methods for analyzing biologically active substances in the composition of a new combined dental gel. Materials and methods. The study object was a dental gel containing “Phytodent” complex tincture (PJSC “CPP Chervona zirka”, Ukraine). Based on the analysis of the composition of the tincture it was proposed to carry out standardization by the amount of biologically active substances, namely flavonoids. Identification was carried out by TLC, while the quantitative determination by absorption spectrophotometry, the ultraviolet and visible method by the reaction with aluminum chloride using the standard method calculated with reference to rutin and the absorbance measurement at 406 nm. Results and discussion. As a result of the research, the methods for the analysis of flavonoids in the composition of the new combined gel have been developed. The spectrophotometric method developed is characterized by specificity, accuracy, precision and linearity with r = 0.9998. One of the important issues when using components of plant origin is their stability both during preparation and storage. Using the method developed the stability of flavonoids has been studied depending on pH changes of the carbomer-based dental gel. Conclusions. It has been determined that the methods developed are easily reproducible and allow to identifying and quantifying flavonoids in the dental gel. It has been found that a stable content of flavonoids is characteristic of the carbomer-based gel neutralized to pH values from 5.0 to 6.0.

scholarly journals Diagnostic Accuracy of Dried Blood Spots Collected on HemaSpot HF Devices Compared to Venous Blood Specimens To Estimate Measles and Rubella Seroprevalence

mSphere ◽  
2021 ◽  
Author(s):  
Christine Prosperi ◽  
Ojas Kaduskar ◽  
Vaishali Bhatt ◽  
Alvira Z. Hasan ◽  
Jeromie W. Vivian Thangaraj ◽  
...  
Keyword(s):  
Venous Blood ◽  
High Sensitivity ◽  
Dried Blood Spots ◽  
Blood Collection ◽  
Serum Samples ◽  
Community Based ◽  
Acceptable Alternative ◽  
Specific Immunoglobulin ◽  
Blood Specimens ◽  
Blood Spot

Dried blood spot (DBS) collection provides an easy, practical, and acceptable alternative to venous blood collection, especially for community-based studies, provided that results from DBS are accurate. We demonstrated high sensitivity and specificity for measles- and rubella-specific immunoglobulin G (IgG) with DBS collected via HemaSpot HF devices compared to serum samples.

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