scholarly journals Application of fluorescent markers for homogeneity assessment of grain mixtures based on maize content

2017 ◽  
Vol 38 (4) ◽  
pp. 505-512 ◽  
Author(s):  
Dominika B. Matuszek ◽  
Krystian Wojtkiewicz
Keyword(s):  
Rhodamine B ◽  
Uv Light ◽  
Test Results ◽  
Fluorescent Substance ◽  
Fluorescent Marker ◽  
Fluorescent Markers ◽  
Fluorescent Method ◽  
Homogeneity Assessment ◽  
Funnel Flow ◽  
Conventional Manner

Abstract The paper presents test results for the assessment of the tracer content in a three-component (green peas, sorghum, maize) feed mixture that is based on the fluorescent method. The homogeneity of mixtures was determined on the basis of the maize content (as the key component), which was treated with fluorescent substance: tinopal, rhodamine B, uranine and eosin. The key components were wet-treated with fluorescent substances with different concentrations. Feed components were mixed in a vertical funnel-flow mixer. 10 samples were collected from each mixed batch. Samples were placed in a chamber equipped with UV light and, then, an image recorded as BMP file was generated. The image was analysed by means of the software programme Patan. On the basis of the analyses conducted, data on the maize content marked with a fluorescent marker were obtained. Additionally, the content of the key component was determined in a conventional manner – using an analytical scale. Results indicate the possibility of using this method for homogeneity assessment of the three-component grain mixture. From these tests, fluorescent substances that can be applied in the case of maize as a key component, together with their minimum concentrations, were identified: tinopal 0.3%, rhodamine B 0.001%.

A Fused Thiophene-S,S-Dioxide-Based Super-Photostable Fluorescent Marker for Lipid Droplets

2020 ◽  
Author(s):  
Masayasu Taki ◽  
Keiji Kajiwara ◽  
Eriko Yamaguchi ◽  
Yoshikatsu Sato ◽  
Shigehiro Yamaguchi
Keyword(s):  
Small Molecules ◽  
Lipid Droplets ◽  
Trajectory Analysis ◽  
Super Resolution ◽  
Fluorescent Marker ◽  
Selective Staining ◽  
Fluorescent Markers ◽  
Biological Studies ◽  
Particle Level

Lipid droplets (LDs) are essential organelle in most eukaryotes, and tracking intracellular LDs dynamics using synthetic small molecules is crucial for biological studies. However, only a limited number of fluorescent markers that satisfy all requirements, such as the selective staining of LDs, high photostability, and sufficient biocompatibility, have been developed. Herein, we report a series of donor-p-acceptor dyes based on the thiophene-containing fused polycyclic scaffold [1]benzothieno[3,2-<i>b</i>][1]benzothiophene (BTBT), in which either or both thiophene rings are oxidized into thiophene-<i>S</i>,<i>S</i>-dioxide to form an electron-accepting building block. Among these dyes, LAQ1 satisfied all the aforementioned requirements, and allowed us capturing ultra-small LDs on the endoplasmic reticulum (ER) membrane by stimulation emission depletion (STED) microscopy with a super-resolution below the diffraction limit of light. Moreover, the extremely high photostability of LAQ1 enabled recording the lipolysis of LDs and the concomitant lipogenesis as well as long-term trajectory analysis of micro LDs at the single particle level in living cells.

Photocatalytic Discolouration of Solutions of Reactive Dyes in the Presence of H2O2

1995 ◽  
Vol 30 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Deng Nansheng ◽  
Tian Shizhong ◽  
Xia Mei
Keyword(s):  
Photochemical Reaction ◽  
Uv Light ◽  
Reactive Dyes ◽  
Solar Light ◽  
Rate Equations ◽  
Oh Radical ◽  
Test Results ◽  
Dye Molecules ◽  
First Order ◽  
Dye Solutions

Abstract Tests for the photocatalytic degradation of solutions of three reactive dyes, Red M-5B, Procion Blue MX-R and Procion Black H-N, in the presence of H2O2 were carried out. When the solutions of the three reactive dyes were irradiated by UV or solar light, the colour of the solutions disappeared gradually. A statistical analysis of the test results indicated a linear relation between the concentration of dyes and the time of irradiation. The discolouration reaction of the solutions was of the first order. Rate equations for the discolouration reactions of dye solutions were developed. The dark reactions or the dye solutions containing H2O2 were very slow, illustrating that the photochemical reaction played a very important role. It was demonstrated that UV light and solar light (300 to 380 nm) photolyzes the HO and that the resulting OH radical reacts with the dye molecules and destroys the chromophore.

scholarly journals DEGRADATION OF AQUEOUS RHODAMINE B BY CATALYSIS ZONE EXTENSION OF MODIFIED DYES SENSITIZED SOLAR CELL.

2017 ◽  
Vol 2 (1) ◽  
Author(s):  
Wirya Sarwana
Keyword(s):  
Solar Cell ◽  
Rhodamine B ◽  
Heating Temperature ◽  
Tio2 Nanotube ◽  
Organic Chemical ◽  
Tio2 Surface ◽  
Test Results ◽  
Heating Treatment ◽  
Amorphous Tio2 ◽  
Rapid Breakdown Anodization

A modified dyes sensitized solar cell (DSSC) having catalysis zone have been successfully developed. The modified DSSC comprise of DSSC zone, employing a rhodamine B as the sensitizer, and catalysis zone, a simple an extension of the TiO2 nanotube film support which was not covered by dyes. The TiO2 nanotube was prepared by rapid breakdown anodization (RBA) method followed by heating treatment of obtained amorphous TiO2 nanotube, and charactetization by UV-Vis DRS, XRD, and SEM. Briefly, the obtained TiO2 has a bundling nanotube morphology, crystalline phase and typical band gap of anatase and rutile mixture (depend on heating temperature). The catalysis zone was tested to treat a water sample containing organic chemical (rhodamine B), as a pollutant model. Test results indicated that the catalysis zone enable to eliminate of rhodamine in the treated water, due to subsequent process starting by generation of super oxide (.O2-) in adjacent TiO2 surface, leading to hydroxyl radical which in turn degrade the rhodamine B. This result indicate that the injected electron from dyes, upon visible light absorption, to conduction band of TiO2 in DSSC zone was successfully migrated to TiO2 surface in catalysis zone.

scholarly journals Fluorescent markers rhodamine B and uranine for Anopheles gambiae adults and matings

2020 ◽  
Author(s):  
Erica i Aviles ◽  
Rachel D Rotenberry ◽  
C Matilda Collins ◽  
Ellen M Dotson ◽  
Mark Q Benedict
Keyword(s):  
Anopheles Gambiae ◽  
Rhodamine B ◽  
Seminal Fluid ◽  
Adult Longevity ◽  
Male Mating ◽  
Potential Contribution ◽  
Laboratory Studies ◽  
Mating Competitiveness ◽  
Fluorescent Markers ◽  
Auto Fluorescence

Abstract Background Marking mosquitoes is vital for mark-release-recapture and many laboratory studies, but their small size precludes the use of methods that are available for larger animals such as unique identifier tags and radio devices. Fluorescent dust is the most commonly used method to distinguish released individuals from the wild population. Numerous colours and combinations can be used, however, dust sometimes affects longevity and behaviour so alternatives that do not have these effects would contribute substantially. Rhodamine B has previously been demonstrated to be useful for marking adult Aedes aegypti males when added to the sugar meal. Unlike dust, this also marked the seminal fluid making it possible to detect matings by marked males in the spermatheca of females. Here, marking of Anopheles gambiae sensu stricto with rhodamine B and uranine was performed to estimate their potential contribution. Methods Two fluorescent markers, rhodamine B and uranine, were dissolved in sugar water and fed to adult An. gambiae. Concentrations that are useful for marking individuals and seminal fluid were determined. The effects on adult longevity, the durability of the marking and detection of the marker in mated females was determined. Male mating competitiveness was also evaluated.Results Rhodamine B marking in adults is detectable for at least three weeks, however uranine marking declines with time and at low doses can be confused with auto-fluorescence. Both can be used for marking seminal fluid which can be detected in females mated by marked males, but, again, at low concentrations uranine-marking is more easily confused with the natural fluorescence of seminal fluid. Neither dye affected mating competitiveness.Conclusions Both markers tested could be useful for field and laboratory studies. Their use has substantial potential to contribute to a greater understanding of the bio-ecology of this important malaria vector. Rhodamine B has the advantage that it appears to be permanent and is less easily confused with auto-fluorescence. The primary limitation of both methods is that sugar feeding is necessary for marking and adults must be held for at least 2 nights to ensure all individuals are marked whereas dusts provide immediate and thorough marking.

scholarly journals Badanie stabilności barwnych znaczników fluorescencyjnych w silnie zasiarczonych wodach złożowych

Nafta-Gaz ◽  
2021 ◽  
Vol 77 (2) ◽  
pp. 82-91
Author(s):  
Katarzyna Wojtowicz ◽  
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Rhodamine B ◽  
Water Reservoir ◽  
Distilled Water ◽  
Test Results ◽  
Fluorescent Tracers ◽  
Reservoir Water ◽  
Relative Standard

The article presents the issues related to the determination of colored fluorescent tracers such as fluorescein, eosin yellowish, rhodamine B and uranine in reservoir waters by spectrophotometric method. For this purpose, the influence of the pH of the solution on the absorption spectra of the tested tracers was checked. Test results show that fluorescein, rhodamine B and uranine are sensitive to changes in the buffer pH, therefore it is advisable to use stable tracer solutions as well as to control and possibly correct pH in further tests. As part of the study, calibration curves of fluorescein, eosin yellowish, rhodamine B and uranine in distilled water, reservoir water A4 and highly sulfated reservoir waters A5 and A6 were plotted and the analytical methods were validated. Analytical validation included determination of linearity, standard deviation and relative standard deviation of the tested tracers solutions. High values of the regression parameters (0.9927–0.9998) of the analyzed tracers prove a good linear fit, while low values of standard deviation and relative standard deviation prove its repeatability and precision. Particular attention was paid to testing the stability of colored fluorescent tracers in highly sulfated reservoir waters. For this purpose, solutions of the tested tracers were prepared at concentrations of 10 mg/dm3 in distilled water, A4 reservoir water and highly sulfated A5 and A6 reservoir waters. Measurements of the tested tracers in the prepared solutions were performed every 2 days over the period of 1 month. The test results show that fluorescein, eosin yellowish, rhodamine B and uranine solutions are stable in the distilled water and A4 reservoir water, while they degrade in the A5 and A6 reservoir waters. Fluorescein and uranine turned out to be the most sensitive, as they degraded completely in the A6 reservoir water after 20 (fluorescein) and 22 (uranine) days. Yellowish eosin and rhodamine B turned out to be slightly more stable in highly sulfated reservoir waters, as they degraded completely in the A6 reservoir water after 24 days.

Use of Rhodamine B as a systemic bait marker for feral cats (Felis catus)

1999 ◽  
Vol 26 (3) ◽  
pp. 281 ◽  
Author(s):  
P. Fisher ◽  
D. Algar ◽  
J. Sinagra
Keyword(s):  
Rhodamine B ◽  
Uv Light ◽  
Field Studies ◽  
Ambient Light ◽  
Captive Population ◽  
Feral Cats ◽  
Hair Samples ◽  
Mystacial Vibrissae ◽  
Single Blind ◽  
Single Blind Trial

A number of cats in a captive population were fed 50 mg of Rhodamine B in non-toxic kangaroo meat baits. Samples of whiskers (mystacial vibrissae) taken 10 days later were examined for fluorescent marking. Examination of hairs for marking was carried out by means of a ‘single blind’ trial, with the investigator having no prior knowledge of which of 36 cats had received the dye. All of the cats that had ingested baits containing the dye were marked. Examining hair samples under ambient light or under a hand-held ultraviolet (UV) light without magnification was not as reliable as examining hair samples under a fluorescence microscope. These results indicate that Rhodamine B acts as a reliable systemic marker of bait consumption in feral cats and has potential application in field studies to assess bait uptake by feral cats.

scholarly journals Rapid Multiplex Detection and Differentiation of Listeria Cells by Use of Fluorescent Phage Endolysin Cell Wall Binding Domains

2010 ◽  
Vol 76 (17) ◽  
pp. 5745-5756 ◽  
Author(s):  
Mathias Schmelcher ◽  
Tatiana Shabarova ◽  
Marcel R. Eugster ◽  
Fritz Eichenseher ◽  
Vincent S. Tchang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Modular Organization ◽  
Binding Assays ◽  
Surface Plasmon Resonance Analysis ◽  
Fluorescent Marker ◽  
Binding Domains ◽  
Cell Wall Binding ◽  
Fluorescent Markers ◽  
Severe Infections ◽  
And Control

ABSTRACT The genus Listeria comprises food-borne pathogens associated with severe infections and a high mortality rate. Endolysins from bacteriophages infecting Listeria are promising tools for both their detection and control. These proteins feature a modular organization, consisting of an N-terminal enzymatically active domain (EAD), which contributes lytic activity, and a C-terminal cell wall binding domain (CBD), which targets the lysin to its substrate. Sequence comparison among 12 different endolysins revealed high diversity among the enzyme's functional domains and allowed classification of their CBDs into two major groups and five subclasses. This diversity is reflected in various binding properties, as determined by cell wall binding assays using CBDs fused to fluorescent marker proteins. Although some proteins exhibited a broad binding range and recognize Listeria strains representing all serovars, others target specific serovars only. The CBDs also differed with respect to the number and distribution of ligands recognized on the cells, as well as their binding affinities. Surface plasmon resonance analysis revealed equilibrium affinities in the pico- to nanomolar ranges for all proteins except CBD006, which is due to an internal truncation. Rapid multiplexed detection and differentiation of Listeria strains in mixed bacterial cultures was possible by combining CBDs of different binding specificities with fluorescent markers of various colors. In addition, cells of different Listeria strains could be recovered from artificially contaminated milk or cheese by CBD-based magnetic separation by using broad-range CBDP40 and subsequently identified after incubation with two differently colored CBD fusion proteins of higher specificity.

scholarly journals Photodegradation of Rhodamine B in Presence of CaO and NiO-CaO Catalysts

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Tobias Kornprobst ◽  
Johann Plank
Keyword(s):  
Visible Light ◽  
Specific Surface ◽  
Surface Area ◽  
Rhodamine B ◽  
Uv Light ◽  
Mechanistic Study ◽  
Light Conditions ◽  
No Adsorption ◽  
Charged Surfaces ◽  
Positively Charged

A CaO catalyst was prepared by mild calcination (650°C) of facilely precipitated Ca(OH)2and compared to an NiO-CaO catalyst obtained from an Ni(OH)2/Ca(OH)2coprecipitate as a precursor. Both catalysts degraded rhodamine B (RB) effectively when exposed to ultraviolet light but exhibited slower degradation under visible light conditions. Under UV light, CaO was more effective than NiO-CaO, while in visible light, the opposite was observed. A mechanistic study revealed no influence of the specific surface area of the catalysts on RB degradation, no adsorption of RB on the positively charged surfaces of the catalysts, and only incomplete degradation of RB. Consequently, both materials represent nonconventional photocatalysts.

Spotted-Wing Drosophila (Diptera: Drosophilidae) Adult Movement, Activity, and Oviposition Behavior in Maine Wild Blueberry (Vaccinium angustifolium; Ericales: Ericaceae)

2019 ◽  
Vol 112 (4) ◽  
pp. 1623-1633 ◽  
Author(s):  
Francis A Drummond ◽  
Elissa Ballman ◽  
Judith A Collins
Keyword(s):  
Oviposition Behavior ◽  
Field Studies ◽  
Crop Canopy ◽  
Drosophila Suzukii ◽  
Dispersal Pattern ◽  
Long Distance ◽  
Vaccinium Angustifolium ◽  
Fluorescent Marker ◽  
Fluorescent Markers ◽  
Wild Blueberry

Abstract Over a period of 5 yr (2012–2016), we conducted laboratory and field studies on activity, movement, and response to trap placement of adult Drosophila suzukii (Matsumura) in wild blueberry, Vaccinium angustifolium Aiton, fields in Maine. When measuring temporal patterns in fruit infestation, we found that D. suzukii females are most active in the morning and that they are 10 times more likely to lay eggs in blueberries at the top of the plant canopy compared with berries located in the lower part of the bush. Flies were found to be more abundant in fruit-bearing (crop) fields compared with pruned (vegetative) fields based on trap capture of adults. They are also most abundant along edges of fields compared with interiors. Trap efficiency is significantly better in traps 1.2 m above the ground and above the crop canopy of this low-growing crop plant than within the crop canopy. Three experiments involving the marking of laboratory-reared flies with fluorescent marker, their release, and capture with traps along a grid in fields suggest that: 1) fluorescent markers do not affect the distance moved of marked flies, 2) dispersal rates are not different between sexes, 3) there is little difference in the dispersal pattern through pruned fields and fruit-bearing fields, and 4) flies disperse at a low rate of 0.1–30 m per day, with an average of 5 m per day, but that long-distance dispersal over 1–2 km is feasible based on statistical model extrapolation.

Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites

2011 ◽  
Vol 9 (01) ◽  
pp. 86-96 ◽  
Author(s):  
Lucy M. Díaz ◽  
Héctor F. Buendía ◽  
Myriam C. Duque ◽  
Matthew W. Blair
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Microsatellite Markers ◽  
Common Bean ◽  
Gene Pool ◽  
Major Gene ◽  
Gene Pools ◽  
Silver Stain ◽  
Fluorescent Marker ◽  
Fluorescent Markers

Colombia, situated at the northern end of the Andes mountains of South America and in proximity to Central America, is an important centre of diversity for common bean (Phaseolus vulgarisL.) that has a mix of cultivated germplasm from both major gene pools (Andean and Mesoamerican) for the species. Microsatellites are a useful marker system for analyzing genetic diversity of this crop and can be analyzed with manual (silver-stain) or automated (ABI) detection systems and using unlabelled or fluorescently labelled markers, respectively. The objectives of this research were to evaluate the genetic diversity of 92 Colombian landraces and gene pool controls with 36 fluorescent and 30 non-fluorescent microsatellite markers and to determine the extent of introgression between the Andean and Mesoamerican gene pools for this germplasm. A comparison of fluorescentversusnon-fluorescent marker systems was performed with 14 loci, which were evaluated with both methods; the fluorescent markers were found to be more precise than the non-fluorescent markers in determining population structure. A combined analysis of 52 microsatellites using the 36 fluorescent markers and 16 non-overlapping, silver-stained markers produced an accurate population structure for the Andean gene pool that separated race Nueva Granada and race Peru genotypes and clearly identified introgression between these races and the gene pools. The results of this research are important for the application of microsatellite markers to diversity analysis in common bean and for the conservation of landraces in Colombia and neighbouring countries of Latin America, where similar germplasm exists and where gene pool or race mixtures also occur.

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