Gold nanoparticles from Celastrus hindsii and HAuCl4: Green synthesis, characteristics, and their cytotoxic effects on HeLa cells

Abstract The extract from Celastrus hindsii (C. hindsii), a plant that naturally grows in the forests of several provinces of Vietnam, has been traditionally used as an alternative medicine for the treatment of inflammation because of its anticancer and antitumor properties. This study reported the green synthesis of stable gold nanoparticles (Au-NPs) derived from HAuCl4 using the extract of C. hindsii as reducing and capping agents. Their particle size could be controlled by adjusting the ratio of the extract to HAuCl4 solution used (1.25%, 2.5%, 3.75%, 5.0%, and 6.25%). The optimal ratio of the extract was 3.75% (Au-NPs-3.75%). The X-ray powder diffraction analysis demonstrated that the Au-NPs was successfully synthesized. Fourier-transform infrared spectroscopy result indicated the possible presence of phenolic acids and flavonoids (acting as reducing agents and potential natural antioxidants). Transmission electron microscopy images showed that the particle diameter of Au-NPs-3.75% varied between 13 and 53 nm (average: ∼30 nm) in its spherical shape. The biosynthesized Au-NPs-3.75% exhibited dose-dependent cytotoxicity against HeLa cells, and the inhibitory concentration (IC50) was 12.5 µg/mL at 48 h. Therefore, Au-NPs that were synthesized from environmentally friendly method without the presence of potentially toxic chemicals were highly possible in biomedical applications.

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Green Synthesis of Gold Nanoparticles Using Aqueous Extract ofGarcinia mangostanaFruit Peels

Journal of Nanomaterials ◽

10.1155/2016/8489094 ◽

2016 ◽

Vol 2016 ◽

pp. 1-7 ◽

Cited By ~ 26

Author(s):

Kar Xin Lee ◽

Kamyar Shameli ◽

Mikio Miyake ◽

Noriyuki Kuwano ◽

Nurul Bahiyah Bt Ahmad Khairudin ◽

...

Keyword(s):

Gold Nanoparticles ◽

Spherical Shape ◽

Garcinia Mangostana ◽

Au Nps ◽

Synthetic Materials ◽

Transmission Electron ◽

Environmentally Safe ◽

Diffraction Peaks ◽

Gold Metal ◽

Peel Extract

The synthesis of gold nanoparticles (Au-NPs) is performed by the reduction of aqueous gold metal ions in contact with the aqueous peel extract of plant,Garcinia mangostana(G. mangostana). An absorption peak of the gold nanoparticles is observed at the range of 540–550 nm using UV-visible spectroscopy. All the diffraction peaks at 2θ= 38.48°, 44.85°, 66.05°, and 78.00° that index to (111), (200), (220), and (311) planes confirm the successful synthesis of Au-NPs. Mostly spherical shape particles with size range of 32.96 ± 5.25 nm are measured using transmission electron microscopy (TEM). From the FTIR results, the peaks obtained are closely related to phenols, flavonoids, benzophenones, and anthocyanins which suggest that they may act as the reducing agent. This method is environmentally safe without the usage of synthetic materials which is highly potential in biomedical applications.

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Preparation and Characterization of Silver and Gold Nanoparticles and Study Influence on Physical Properties of PVA/PVP nanocomposites

JOURNAL OF ADVANCES IN PHYSICS ◽

10.24297/jap.v13i1.5626 ◽

2017 ◽

Vol 13 (1) ◽

pp. 4628-4639

Author(s):

A. M. Abdelghany ◽

Mahrous. S. Meikhail ◽

A. A. ALdhabi

Keyword(s):

Gold Nanoparticles ◽

Spherical Shape ◽

X Ray Diffraction ◽

Ag Nps ◽

Au Nps ◽

Silver And Gold Nanoparticles ◽

Plant Extraction ◽

Chenopodium Murale ◽

Transmission Electron

Gold nanoparticles (Au NPs) and Silver nanoparticles (Ag NPs) ware prepared by â€œgreenâ€ synthesis extraction using the Chenopodium murale leaf extract, the obtained (Ag NPs and Au NPs) were investigated by UV/Vis. absorption spectroscopy, transmission electron microscopy, Zetasizer, XRD. The plant extraction leads to produce nanoparticles of spherical shape with size range from 4 to 22 nm.Â Polyvinyl alcohol (PVA) and polyvinyl Pyrolidone (PVP) blend with mixed silver and gold nanoparticles were prepared by casting method. Amorphous feather of doping polymers blend was characterized by X-ray diffraction. Significant changes within the polymer matrix were monitored from infrared spectroscopy which indicates the interaction between polymer blend and mixed nanoparticles. Both indirect and direct optical energy gaps are calculated and discussed.

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Green Synthesis of Gold Nanoparticles using Aqueous Garlic (Allium sativum L.) Extract, and Its Interaction Study with Melamine

BULLETIN OF CHEMICAL REACTION ENGINEERING AND CATALYSIS ◽

10.9767/bcrec.12.2.770.212-218 ◽

2017 ◽

Vol 12 (2) ◽

pp. 212 ◽

Cited By ~ 8

Author(s):

Yoki Yulizar ◽

Harits Atika Ariyanta ◽

Lingga Abduracman

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Allium Sativum ◽

Synthesis Method ◽

Spherical Shape ◽

Phytochemical Analysis ◽

Interaction Study ◽

Stabilizing Agents ◽

Selected Area Electron Diffraction ◽

Transmission Electron

Gold nanoparticles (AuNPs) have been successfully prepared by green synthesis method using aqueous extract of garlic with the Latin name of Allium sativum L. (ASL) as a reducing and stabilizing agents. Identification of active compounds in aqueous ASL extract was conducted by phytochemical analysis and Fourier transform infrared (FTIR) spectroscopy, while the synthesized AuNPs were characterized using UV-Vis spectrophotometer and transmission electron microscopy-selected area electron diffraction (TEM-SAED). The AuNPs formation was optimized at aqueous ASL extract concentration of 0.05%, HAuCl4 concentration of 2.0×10-4 M, and pH of 3.6. The optimized AuNPs was characterized using TEM, and has a spherical shape with particle size of 15±3 nm. The particles were also stable up until one month. The synthesized AuNPs has been studied its interaction with melamine, and showed the optimum pH of interaction at 3.6. Copyright © 2017 BCREC GROUP. All rights reservedReceived: 13rd November 2016; Revised: 3rd January 2017; Accepted: 10th February 2017How to Cite: Yulizar, Y., Ariyanta, H.A., Abdurrachman, L. (2017). Green Synthesis of Gold Nanoparticles using Aqueous Garlic (Allium sativum L.) Extract, and Its Interaction Study with Melamine. Bulletin of Chemical Reaction Engineering & Catalysis, 12 (2): 212-218 (doi:10.9767/bcrec.12.2.770.212-218)Permalink/DOI: http://dx.doi.org/10.9767/bcrec.12.2.770.212-218

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Green Synthesis of Silver and Gold Nanoparticles via Sargassum serratifolium Extract for Catalytic Reduction of Organic Dyes

Catalysts ◽

10.3390/catal11030347 ◽

2021 ◽

Vol 11 (3) ◽

pp. 347

Author(s):

Beomjin Kim ◽

Woo Chang Song ◽

Sun Young Park ◽

Geuntae Park

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Catalytic Reduction ◽

Organic Dyes ◽

Low Cost ◽

Inorganic Nanoparticles ◽

X Ray ◽

Selected Area Electron Diffraction ◽

Transmission Electron ◽

Sargassum Serratifolium

The green synthesis of inorganic nanoparticles (NPs) using bio-materials has attained enormous attention in recent years due to its simple, eco-friendly, low-cost and non-toxic nature. In this work, silver nanoparticles (AgNPs) and gold nanoparticles (AuNPs) were synthesized by the marine algae extract, Sargassum serratifolium (SS). The characteristic studies of bio-synthesized SS-AgNPs and SS-AuNPs were carried out by using ultraviolet–visible (UV–Vis) absorption spectroscopy, dynamic light scattering (DLS), high-resolution transmission electron microscope (HR-TEM), selected area electron diffraction (SAED), energy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). Phytochemicals in the algae extract, such as meroterpenoids, acted as a capping agent for the NPs’ growth. The synthesized Ag and Au NPs were found to have important catalytic activity for the degradation of organic dyes, including methylene blue, rhodamine B and methyl orange. The reduction of dyes by SS-AgNPs and -AuNPs followed the pseudo-first order kinetics.

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Effects of Different Surfactant Charges on the Formation of Gold Nanoparticles by the LASiS Method

Materials ◽

10.3390/ma14112937 ◽

2021 ◽

Vol 14 (11) ◽

pp. 2937

Author(s):

Muhammad Zulfajri ◽

Wei-Jie Huang ◽

Genin-Gary Huang ◽

Hui-Fen Chen

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Gold Nanoparticles ◽

Laser System ◽

Synthesis Process ◽

Au Nps ◽

Laser Fluences ◽

Transmission Electron ◽

Vis Spectroscopy ◽

532 Nm

The laser ablation synthesis in solution (LASiS) method has been widely utilized due to its significant prospects in laser microprocessing of nanomaterials. In this study, the LASiS method with the addition of different surfactant charges (cationic CTAB, nonionic TX-100, and anionic SDS) was used to produce Au NPs. An Nd:YAG laser system at 532 nm excitation with some synthetic parameters, including different laser fluences, ablation times, and surfactant concentrations was performed. The obtained Au NPs were characterized by UV-Vis spectroscopy, transmission electron microscopy, and zeta potential analyzer. The Au NPs exhibited the maximum absorption peak at around 520 nm for all samples. The color of Au NPs was changed from red to reddish by increasing the laser fluence. The surfactant charges also played different roles in the Au NPs’ growth during the synthesis process. The average sizes of Au NPs were found to be 8.5 nm, 5.5 nm, and 15.5 nm with the medium containing CTAB, TX-100, and SDS, respectively. Besides, the different surfactant charges induced different performances to protect Au NPs from agglomeration. Overall, the SDS and CTAB surfactants exhibited higher stability of the Au NPs compared to the Au NPs with TX-100 surfactant.

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Effect of Gold Nanoparticles Size on Detection of Methylphosphonic Acid Hydrolysis Product of Nerve Agent

Research Journal of Chemistry and Environment ◽

10.25303/257rjce0121 ◽

2021 ◽

Vol 25 (7) ◽

pp. 1-7

Author(s):

Fellyzra Elvya Pojol ◽

Buong Woei Chieng ◽

Keat Khim Ong ◽

Rashid Jahwarhar Izuan Abd ◽

Mohd Junaedy Osman ◽

...

Keyword(s):

Gold Nanoparticles ◽

Inverse Method ◽

Direct Method ◽

Hydrolysis Product ◽

Spherical Shape ◽

Methylphosphonic Acid ◽

Visible Spectroscopy ◽

Transmission Electron ◽

Citrate Reduction ◽

Chloride Trihydrate

Citrate reduction of gold (III) chloride trihydrate (HAuCl4) is commonly used method to synthesise citrate-capped gold nanoparticles (cit-AuNPs). In this study, the sequence of reagents addition was modified (“inverse” method) to synthesise smaller size of cit-AuNPs than the standard Turkevich method (“direct” method). Ultraviolet-visible spectroscopy (UV-vis) and field emission transmission electron microscopy (FETEM) confirmed the formation of cit-AuNPs. The cit-AuNPs synthesized using “inverse” method are smaller in size (14.0 ± 3.03 nm) with uniform spherical shape compared to “direct” method (23.5 ± 7.52 nm). Smaller particles size of cit-AuNPs provide higher efficiency and sensitivity for detection of methylphosphonic acid (MPA) via colorimetric incorporated with image processing with a linear range from 2.5 to 12.5 mM and a low detection limit of 6.28 mM at shorter detection period (24 to 30 s).

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Preparation of Cellulose Acetate Ultra-Fine Fibers Containing Quasi-Spherical Gold Nanoparticles by Electrospinning Method

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.391-392.400 ◽

2011 ◽

Vol 391-392 ◽

pp. 400-403

Author(s):

Dong Mei Zhao ◽

Li Guo Sun ◽

Li Li Lv ◽

Jian Li

Keyword(s):

Electron Microscopy ◽

Gold Nanoparticles ◽

Cellulose Acetate ◽

Trisodium Citrate ◽

X Ray Diffraction ◽

Au Nps ◽

Transmission Electron ◽

Electrospinning Method ◽

Before And After ◽

Spherical Gold Nanoparticles

Quasi-spherical gold nanoparticles(Au NPs) prepared by trisodium citrate reduction of HAuCl4were dispersed into cellulose acetate(CA) ultra-fine fibers by electrospinning. The optical properties of Au NPs before and after electrospinning were measured by UV-vis spectrometer. The morphology and distribution of Au NPs in CA ultra-fine fibers were observed by transmission electron microscopy (TEM). The morphology and diameter of Au NPs/CA fibers were studied by scanning electron microscopy (SEM). The crystallinity change of CA fiber before and after adding Au NPs was characterized by X-ray diffraction (XRD).

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Green Nanoparticles for Mosquito Control

The Scientific World JOURNAL ◽

10.1155/2014/496362 ◽

2014 ◽

Vol 2014 ◽

pp. 1-6 ◽

Cited By ~ 27

Author(s):

Namita Soni ◽

Soam Prakash

Keyword(s):

Gold Nanoparticles ◽

Mosquito Control ◽

Spherical Shape ◽

Probit Analysis ◽

Bark Extract ◽

Green Method ◽

Silver And Gold Nanoparticles ◽

Transmission Electron ◽

Uv Visible ◽

Filariasis Vector

Here, we have used the green method for synthesis of silver and gold nanoparticles. In the present study the silver (Ag) and gold (Au) nanoparticles (NPs) were synthesized by using the aqueous bark extract of Indian spice dalchini (Cinnamomum zeylanicum) (C. zyelanicumorC. verumJ. Presl). Additionally, we have used these synthesized nanoparticles for mosquito control. The larvicidal activity has been tested against the malaria vectorAnopheles stephensiand filariasis vectorCulex quinquefasciatus. The results were obtained using UV-visible spectrophotometer and the images were recorded with a transmission electron microscope (TEM). The efficacy tests were then performed at different concentrations and varying numbers of hours by probit analysis. The synthesized AgNPs were in spherical shape and average sizes (11.77 nm AgNPs and 46.48 nm AuNPs). The larvae ofAn. stephensiwere found highly susceptible to the synthesized AgNPs and AuNPs than theCx. quinquefasciatus. These results suggest that theC. zeylanicumsynthesized silver and gold nanoparticles have the potential to be used as an ideal ecofriendly approach for the control of mosquito.

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A new strategy for the green synthesis of chondroitin sulfate-reduced gold nanoparticles; in vitro evaluation of synthesized nanoparticles

Bioimpacts ◽

10.34172/bi.2020.28 ◽

2020 ◽

Vol 10 (4) ◽

pp. 217-226

Author(s):

Maryam Asariha ◽

Azam Chahardoli ◽

Farshad Qalekhani ◽

Mahnaz Ghowsi ◽

Mehdi Fouladi ◽

...

Keyword(s):

Gold Nanoparticles ◽

Antibacterial Activity ◽

Green Synthesis ◽

Chondroitin Sulfate ◽

Blood Compatibility ◽

Spherical Shape ◽

Hemolysis Assay ◽

Wide Range ◽

New Strategy

introduction: The application of gold nanoparticles (GNPs) in medicine is expanding as an effective therapeutic and diagnostic compound. Different polysaccharides with high biocompatibility and hydrophilic properties have been used for synthesis and capping of GNPs. Chondroitin sulfate (CHS) as a polysaccharide possesses a wide range of biological functions e.g. anti-oxidant, anti-inflammation, anti-coagulation, anti-atherosclerosis, anti-thrombosis with insignificant immunogenicity and has not been used for the green synthesis of GNPs. Methods: GNPs were synthesized using CHS, and their physicochemical properties were evaluated. The antibacterial activity of CHS-GNPs was estimated against both gram-positive and gram-negative bacteria. The cytotoxicity of CHS and CHS-GNPs was obtained by MTT (3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide) test, and the electrocatalytic activity of CHS-GNPs was investigated. The blood compatibility was evaluated by the in vitro hemolysis assay. Results: The absorption band at 527 nm reveals the reduction of Au3+ into GNPs. The transmission electron microscopy (TEM) image displays the spherical shape of GNPs in the range of 5.8–31.4 nm. The CHS and CHS-GNPs at 300 µg/mL revealed a maximum DPPH (1, 1-diphenyl-2-picrylhydrazyl) scavenging activity of 73% and 65%, respectively. CHS-GNPs showed antibacterial activity against Bacillus subtilis, while CHS has no antibacterial activity. CHS-GNPs exhibited a cytotoxicity effect against MDA-MB-468 and βTC3 cancer cell lines, and the electrochemical study indicated a significant increase in electrocatalytic properties of CHS-GNPs coated electrode compared by the bare electrode. The hemolysis test proved the blood compatibility of CHS-GNPs. Conclusion: The results indicate the advantages of using CHS to produce blood-compatible GNPs with antioxidant, cytotoxic, and electrochemical properties.

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Green Synthesis of Gold Nanoparticles Using Longan Polysaccharide and their Reduction of 4-nitrophenol and Biological Applications

NANO ◽

10.1142/s1793292020500022 ◽

2020 ◽

Vol 15 (01) ◽

pp. 2050002 ◽

Cited By ~ 6

Author(s):

Xiaoyu Zhang ◽

Liyuan Fan ◽

Yanshuai Cui ◽

Tianming Cui ◽

Shengfu Chen ◽

...

Keyword(s):

Gold Nanoparticles ◽

Green Synthesis ◽

Metallic Nanoparticles ◽

High Catalytic Activity ◽

Synthetic Method ◽

Biological Applications ◽

Au Nps ◽

Stabilizing Agents ◽

Highly Dispersed ◽

Negative Zeta Potential

The green synthesis of gold nanoparticles (Au NPs) for catalytic and biological applications has been drawing great attention. To compare with plant extracts, the polysaccharides may be good reducing and stabilizing agents. In this work, we describe the preparation of longan polysaccharide stabilized gold nanoparticles (Aun-LP NPs) by reduction of gold ions using a green synthetic method. The formation of gold nanoparticles (Au NPs) was confirmed by UV-Vis spectra. TEM showed that Au NPs had a small size (7.8–15.6[Formula: see text]nm) and were highly dispersed without any aggregation. XPS confirmed that the surface elemental composition of Aun-LP NPs was C, O, and Au. DLS demonstrated that Aun-LP NPs had good stability and negative zeta potential. In addition, Aun-LP NPs had high catalytic activity for the reduction of 4-nitrophenol. More importantly, Aun-LP NPs had ignorable cytotoxicity towards HeLa cells and showed good antioxidant activity. Taken together, the results indicated that longan polysaccharide can be used as reducing agents and stabilizers for the preparation of metallic nanoparticles, and the product had wide applications.

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