Effect of pH on Protein Extraction from Mahaleb Kernels and Functional Properties of Resulting Protein Concentrate

2019 ◽  
Vol 15 (7) ◽  
Author(s):  
Melih Güzel ◽  
Mehtap Çelik ◽  
Metin Yildirim
Keyword(s):  
Functional Properties ◽  
Protein Extraction ◽  
Chemical Properties ◽  
Protein Concentrate ◽  
Effect Of Ph ◽  
Ph Values ◽  
Precipitation Ph ◽  
Foaming Capacity ◽  
Water Holding ◽  
And Chemical Properties

AbstractThe aims of this research were to examine the effect of pH on extraction of proteins from mahaleb (Prunus mahaleb L.) kernels, and to investigate the functional properties of resulting protein concentrate. The optimum pH values for the protein extraction and precipitation were determined as 10.0 and 4.5, respectively. The protein concentrate containing 92.73% dry matter, 6.29% ash, 6.02% carbohydrate, 1.42% fat and 73.11% protein was produced by using these extraction and precipitation pH values. Water-holding capacity, oil-holding capacity and the least gelling concentration of the protein concentrate were 2.81 g water/g, 1.66 g oil/g and 12%, respectively. Moreover, emulsifying activity and stability indices, foaming capacity and stability of protein concentrate were 27.21 m2/g, 81.05 min, 43.75% and 71.33% (after 30 min), respectively. The functional and chemical properties of the protein concentrate indicate that it may find application as functional ingredient for various food products.

scholarly journals PENGARUH pH EKSTRAKSI TERHADAP RENDEMEN, SIFAT FISIKO-KIMIA DAN FUNGSIONAL KONSENTRAT PROTEIN KACANG GUDE (Cajanus cajan (L.) Millsp.)

2018 ◽  
Vol 7 (1) ◽  
pp. 1
Author(s):  
Hesti Pratiwi ◽  
Ni Luh Ari Yusasrini ◽  
I Nengah Kencana Putra
Keyword(s):  
Functional Properties ◽  
Emulsion Stability ◽  
Chemical Properties ◽  
Pigeon Pea ◽  
Water Holding Capacity ◽  
Protein Concentrate ◽  
Pea Protein ◽  
Emulsion Capacity ◽  
Foaming Capacity ◽  
Water Holding

The purpose of this research are to know the effect of pH extraction on yield, physico-chemical and functional properties of pigeon pea protein concentrate, and also to know the appropriate pH extraction to obtain the best yield and properties of pigeon pea protein concentrate. The research was using Randomized Complete Design with pH extraction treatment which consist of five levels were 9; 9,5; 10; 10,5; and 11. The parameters were observed consist of yield, physical properties (lightness), chemical properties (protein and moisture), and protein’s functional properties (water holding capacity, oil holding capacity, emulsion capacity and stability, and foaming capacity and stability). The result showed that pH extraction gave a significant effect to protein and moisture, and gave very significant effect to yield, lightness (L), water holding capacity, oil holding capacity, emulsion capacity, emulsion stability, foaming capacity and foaming stability of pigeon pea protein concentrate. The best properties of protein concentrate was found at sample which treatment of pH 10 with a yield 17,29% (db), lightness (L) 18,84, protein 86,15% (db), moisture 7,96% (db), water holding capacity 1,96 ml H2O/g solid, oil holding capacity 2,62 ml oil/g solid, emulsion capacity 54,67%, emulsion stability 54,83%, foaming capacity 100%, and foaming stability 38,19%.

scholarly journals Functional properties of protein isolates from camelina (Camelina sativa (L.) Crantz) and flixweed (sophia, Descurainis sophia L.) seed meals

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Na Thi Ty Ngo ◽  
Fereidoon Shahidi
Keyword(s):  
Functional Properties ◽  
Protein Extraction ◽  
Protein Profile ◽  
Protein Solubility ◽  
Protein Isolate ◽  
Absorption Capacity ◽  
Foaming Properties ◽  
Oil Absorption ◽  
Protein Isolates ◽  
Water Holding

AbstractCamelina and flixweed (sophia) seed protein isolates were prepared using both the conventional extraction and ultrasonic-assisted extraction methods at 40 kHz for 20 min, and their functional properties investigated. SDS-PAGE showed that both ultrasound-assisted and conventional extractions resulted in a similar protein profile of the extract. The application of ultrasound significantly improved protein extraction/content and functional properties (water holding capacity, oil absorption capacity, emulsifying foaming properties, and protein solubility) of camelina protein isolate and sophia protein isolate. The water-holding and oil absorption capacities of sophia protein isolate were markedly higher than those of camelina protein isolate. These results suggest that camelina protein isolate and sophia protein isolate may serve as natural functional ingredients in the food industry. Graphical Abstract

Extracting Tenebrio molitor protein while preventing browning: effect of pH and NaCl on protein yield

2017 ◽  
Vol 3 (1) ◽  
pp. 21-31 ◽  
Author(s):  
L. Yi ◽  
M.A.J.S. Van Boekel ◽  
C.M.M. Lakemond
Keyword(s):  
Protein Extraction ◽  
Protein Solubility ◽  
Tenebrio Molitor ◽  
Extraction Yield ◽  
Water Soluble ◽  
Sodium Bisulphite ◽  
Effect Of Ph ◽  
Alternative Protein ◽  
Food Applications ◽  
Precipitation Ph

The potential of insects as an alternative protein source for food applications was investigated by studying the effect of pH and NaCl on extraction yield of water-soluble proteins from Tenebrio molitor, while preventing browning due to polyphenol oxidation. Minimum protein solubility (29.6%) was at pH=4-6 and maximum (68.6%) at pH=11. Protein extraction at 0.1 M NaCl, pH=10 gave a recovery of 100%. The observed increase in browning at pH=8-11 corresponded to a lower monomeric phenol content. Sodium bisulphite (studied from 0.5-4%) could prevent browning, whereas ascorbic acid (studied in the range 0.01-0.04%) could not prevent as strong as sodium bisulphite. After acid precipitation (pH=4) an isolate with a protein content of 74% (yield of 22% of total protein) was obtained. It was observed that proteins from T. molitor behave more or less the same as proteins from meat and fish with respect to aqueous extraction, which is of relevance for food industry when using T. molitor protein as an ingredient in food products.

Antioxidative Activity and Functional Properties of Hydrolysates of Camellia Seed Meal Treated with Trypsin

2012 ◽  
Vol 554-556 ◽  
pp. 1174-1177 ◽  
Author(s):  
Ying Sun ◽  
Guo Qin Liu ◽  
Xin Qi Liu
Keyword(s):  
Functional Properties ◽  
Antioxidative Activity ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Seed Meal ◽  
Degree Of Hydrolysis ◽  
Foaming Capacity ◽  
Ph Range ◽  
Water Holding ◽  
As Solubility

Antioxidative activity and functional properties of hydrolysates prepared from defatted camellia seed meal(DCSM), using trypsin, with a degree of hydrolysis(DH) of 21.69% were determined. At this DH, the hydrolysates show the highest hydroxyl and superoxide anion radical scavenging activity (over 69.15 % and 78.22%, respectively). The functionalities of hydrolysates such as solubility, water-holding capacity,emulsifying activity, foaming capacity and foaming stability are remarkably improved at all test pH range. These results indicate that the DCSM hydrolysised by trypsin to produce hydrolysates with desirable antioxidative activity and functional properties.

scholarly journals Cotton Gin Compost as a Substrate Component in Container Production of Nursery Crops

2005 ◽  
Vol 23 (3) ◽  
pp. 118-122 ◽  
Author(s):  
Brian E. Jackson ◽  
Amy N. Wright ◽  
David M. Cole ◽  
Jeff L. Sibley
Keyword(s):  
Chemical Properties ◽  
Total Porosity ◽  
Root Systems ◽  
Growth Indices ◽  
Periodic Growth ◽  
Air Space ◽  
Water Holding ◽  
And Chemical Properties ◽  
Cotton Gin

Abstract Shoot and root growth of plants grown in substrate blends containing cotton gin compost (CGC) were compared to plants grown in a traditional pine bark (PB) blend. In 2002 ‘Winter Gem’ boxwood, ‘Firepower’ dwarf nandina, and ‘Midnight Flare’ azalea were potted in four substrate blends containing by volume 6:1 PB:sand (S), 4.5:1.5:1 PB:CGC:S, 1:1 PB:CGC, or 1.5:4.5:1 PB:CGC:S. Plants were grown for nine months on a container pad in Auburn, AL. In 2003–2004 this study was repeated with ‘Renee Mitchell’ azalea as a replacement for ‘Midnight Flare’ azalea. Periodic growth measurements and a final visual root ball evaluation were conducted in each experiment. Substrates were analyzed to determine physical properties (air space, water holding capacity, total porosity, and bulk density) and chemical properties (pH and electrical conductivity). In both experiments, growth indices of all cultivars in substrates containing CGC were similar to or greater than those of the PB:S control blend. Visual quality of root systems was similar for all plants across all substrates.

scholarly journals Effect of pH on Soil Chemical Properties and Maize Performance in Abakaliki, Nigeria

2022 ◽  
Author(s):  
J.N. Nwite ◽  
A.J. Ajana ◽  
I. Alinchi
Keyword(s):  
Chemical Properties ◽  
Maize Yield ◽  
Laboratory Method ◽  
The State ◽  
Soil Chemical Properties ◽  
Soil Productivity ◽  
Effect Of Ph ◽  
Traditional System ◽  
Ph Values ◽  
Nutrients Availability

Background: Low soil productivity in Nigeria and Africa sub sahara is considered as one of the major causes of food insecurity and under nutrition. This area is considered among the most regions affected by acidity on soil nutrients optimization. Despite of its severity, there is limited knowledge about limiting effect of pH on nutrients availability and maize yield in the state. Evolving a technology that does not depend on traditional system of amendment for pH assessment for increased soil productivity must first of all go through a good knowledge of the nature and distribution of soil studied. The present study aimed to evaluate three pH (4, 7 and 9) values on soil chemical properties in the state and assess maize performance under these conditions in order to control and manage pH related problems. Methods: In the field-laboratory investigation during 2018-2019 at different localities of Ebonyi State were surveyed. Three sampling localities were selected based on geographical situation, climate and local condition. In the laboratory, the soil samples of natural stock collected were processed for mean pH values and confirmed with pH meter using standard laboratory method. Maize was used as test crop to assess effect of pH on its performance. Result: Our investigations in Ebonyi State have allowed us to inventory three pH values. Among the inventoried pH values, some are regarded adverse for soil productivity. The implication of soil pH on nutrients availability and maize performance was discussed. The current work will be a complementary contribution of detailed study of effect of pH on soil productivity.

scholarly journals Flours and starches made from cassava (yuca), yam, sweet potatoes and ñampi: functional properties and possible applications in the food industry

2016 ◽  
Vol 29 (5) ◽  
pp. 86 ◽  
Author(s):  
Pedro Vargas-Aguilar
Keyword(s):  
Functional Properties ◽  
Nutritional Value ◽  
Chemical Properties ◽  
Fiber Content ◽  
Gluten Free ◽  
Fat Absorption ◽  
Sweet Potatoes ◽  
Fried Foods ◽  
Physical And Chemical ◽  
And Chemical Properties

<p class="p1">A review was carried out of the physical and chemical properties of flours and starches made from cassava, yam, ñampi, and sweet potatoes. Amyloseamylopectin content, viscosity, nutritional value and fiber content were studied to associate them with their functional and technical-functional properties. A relationship was found between amylose-amylopectin, viscosity and fiber content and digestibility of those starches, as well as with glycemic index values. </p><p class="p1">It was found that it is possible to introduce modifications through fermentation to enhance the nutritional value of these starches. Fermentation allows the production of gluten-free bread and energy drinks as an option to diversify products. It was also found that there is a relationship between starch viscosity and a reduction of fat absorption in fried foods. </p>

scholarly journals Production of Denatured Whey Protein Concentrate at Various pHfrom Wastewater of Cheese Industry

2021 ◽  
Vol 41 (2) ◽  
pp. 161
Author(s):  
Robi Andoyo ◽  
Anindya Rahmana Fitri ◽  
Ratih Siswanina Putri ◽  
Efri Mardawati ◽  
Bambang Nurhadi ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Whey Protein ◽  
Functional Properties ◽  
Heat Treatment Condition ◽  
Whey Protein Concentrate ◽  
Protein Concentrate ◽  
Cheese Industry ◽  
Ph Level ◽  
Water Holding ◽  
Physicochemical And Functional Properties

Wastewater produced from cheese industry is rich in biological component such as whey protein, fat and lactose. Whey protein is the residual liquid of cheese making process with a high protein efficiency ratio. The wastewater source used in this study was whey liquid from cheese processing industry located at West Java, Indonesia. Conversion of soluble whey protein into whey protein microparticle is required to produce food with nutritional value that can be adjusted to the needs of the specific target with high digestibility and palatability. Whey protein was collected by separation technique through heat treatment at specific condition. This was done by changing the heat treatment condition and pH of the samples. Changing the pH of the samples before heat treatment affect the ionic strength of the whey protein hence, altering the properties of the concentrate. This study aims to produce whey protein concentrate heated at various pH level and to observe physicochemical and functional properties of the concentrates. The method used in this research was a descriptive method conducted on three treatments and two replications namely whey protein concentrate production in a pH condition 6.4; 6.65; and 7.0. The parameters observed were physicochemical and functional properties. Furthermore, the result showed that there were decrease in protein content, along with the increasing pH before heat treatment. Microstructure image (SEM) showed a finer particles with the increasing pH. Meanwhile, solubility of the rehydrated samples tends to increase along with the increasing pH. The measurement of functional properties of the samples showed that denatured whey protein produced at different pH before heat treatment have different water holding capacity and a tendency to form bonds between protein particles thereby increasing the viscosity value. These physicochemical and functional properties were suitable for denatured whey protein to be used as a texture controller in whey protein based-food production.

Soils of Thotapalli Major Irrigation Project of North- Coastal Andhra Pradesh: Physical and Chemical Properties

Agropedology ◽  
2019 ◽  
Vol 29 (1) ◽  
Author(s):  
K. Himabindu ◽  
◽  
P. Gurumurthy ◽  
P.R.K. Prasad ◽  
◽  
...  
Keyword(s):  
Andhra Pradesh ◽  
Pore Space ◽  
Chemical Properties ◽  
Physical And Chemical Properties ◽  
Clay Loam ◽  
Irrigation Project ◽  
Maximum Water ◽  
Physical And Chemical ◽  
Water Holding ◽  
And Chemical Properties

Physical and chemical properties of the soils were assessed for the ayacut area of Thotapalli major irrigation project of North-Coastal Andhra Pradesh. Six representative soil pedons were studied in Devarapalli, Gujjangivalasa, Patikivalasa, Gangada, Aamiti and Maddivalasa villages falling in ayacut area of Thotapalli major irrigation project. The soil texture ranged from sandy loam to sandy clay loam in upland pedons while sandy clay loam to clay in pedons under cultivated plains. In general, relatively low bulk density was recorded at surface horizons compared to sub-surface layers. Maximum water holding capacity, pore space and volume expansion increased with soil depth. The soils were acidic to alkaline in reaction, non-saline, low to medium in organic carbon content. The CEC ranged from 6.4 to 32.5 cmol (p+) kg-1. The soil exchange complex was dominated by calcium followed by magnesium, sodium and potassium. The soils were found to be low in nitrogen, low to medium in phosphorus and medium to high in available potassium status. Manganese and copper were sufficient but zinc and iron were deficient in soils. The total silica (SiO2) content of pedons varied from 48.10 to 71.40 per cent. Sesquioxide (R2O3) and the molar ratio of SiO2 / R2O3 ranged from 19.01 to 32.4 per cent and varied from 3.74 to 6.59, respectively in different pedons. The SiO2 / Al2O3 ratio and the SiO2 / Fe2O3 ratio varied from 4.43 to 7.65 and from 13.7 to 47.8, respectively. A significant positive correlation of clay content with cation exchange capacity (CEC), maximum water holding capacity (MWHC) was observed. Sand content was positively correlated with silica and negatively correlated with MWHC and volume expansion. Soil organic carbon had a significant positive correlation with soil pore space, available nitrogen, phosphorous, potassium, zinc, manganese, copper and iron, but a negative correlation with soil bulk density. Soil pH was positively correlated with CaCO3 but negatively correlated with available phosphorous, potassium, zinc, manganese, and copper and iron. Soil CaCO3 content was negatively correlated with available phosphorous, potassium, zinc, manganese, copper and iron of the soil.

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