Cluster of Differentiation (CD) Antigen

2016 ◽  
Author(s):  
Douglas M. Templeton ◽  
Michael Schwenk ◽  
Reinhild Klein ◽  
John H. Duffus
Keyword(s):  
Cd Antigen ◽  
Cluster Of Differentiation

scholarly journals Functional and phenotypic analyses of porcine gut immune cells immunized by oral administration of F4ac+nonenterotoxigenic Escherichia coli strains

2012 ◽  
Vol 47 (No. 12) ◽  
pp. 333-341 ◽  
Author(s):  
N. Vijtiuk ◽  
K. Trutin-Ostovič ◽  
T. Balenovič ◽  
M. Popovič ◽  
I. Valpotič
Keyword(s):  
T Cells ◽  
Immune Cells ◽  
Antigen Expression ◽  
Pokeweed Mitogen ◽  
Vaccination Programs ◽  
Etec Strain ◽  
Cd Antigen ◽  
Species Specific ◽  
Cluster Of Differentiation

The aim of this study was to determine the priming effect of experimentally inoculated non-ETEC strains (2407, 1466) on gastrointestinal mucosal lymphocytes. Five 4-week-old pigs per group were orally inoculated with either F4ac<sup>+</sup>(1466 or 2407) or F4&ndash; (1467) non-ETEC strains. The control pigs were given broth containing 1.2% sodium bicarbonate. At postinoculation Day 6 the pigs were killed, their gut lymphocytes were isolated, and their responsiveness was tested in vitro with F4 antigen, peptidoglycan monomer (PGM), pokeweed mitogen (PWM), phytohemagglutinin (PHA) and lipopolysaccharide (LPS). Additionally, the patterns of cluster of differentiation (CD) antigen expression on T and B cells in the single cell suspensions from JLP, IPP, and MLN were determined by flow cytometry using anti-swine CD-specific monoclonal antibodies. F4ac+ non-ETEC strain 2407 and, to a lesser extent 1466, activated lymphocytes from PP and MLN to respond better to common mitogens (PHA, PWM, LPS), purified fimbrial (F4ac) antigen or immunologic response modifier (PGM). An increase of CD2a<sup>+</sup>and CD8a<sup>+</sup>T cells in JLP, and species-specific SWC1<sup>+</sup>T cells in MLN (P &lt; 0.05) was detected in 2407-treated pigs. In conclusion, inoculation with non-ETEC strain 2407 exhibited stimulatory properties to porcine gut immune cells, and thus, could be used in the vaccination programs to control the postweaning colibacillosis in pigs.

Aberrant Expression of CD Markers in Acute Myeloid Leukaemia

2018 ◽  
Vol 2 (01) ◽  
pp. 14-16
Author(s):  
Abul Kalam Azad ◽  
Md. Rafiquzzaman Khan ◽  
ABM Hasan Habib ◽  
Md. Abdul Wadud Miah ◽  
Masuda Begum
Keyword(s):  
Flow Cytometry ◽  
Acute Myeloid Leukaemia ◽  
Myeloid Leukaemia ◽  
Acute Leukaemia ◽  
Antigen Expression ◽  
Aberrant Expression ◽  
Female Ratio ◽  
Cd Antigen ◽  
Cd Markers ◽  
Acute Myeloid

Background: Aberrant expression of cluster differentiation (CD) antigen marker is associated with poor outcome of acute leukaemia. Objective: Aim of this study is to determine the frequency and pattern of aberrant expression of CD markers in acute myeloid leukaemia patients in Bangladesh. Methods: This retrospective data analysis was conducted in the Department of Haematology, Bangabandhu Sheikh Mujib Medical University (BSMMU) to assess the frequency of aberrant CD antigen expression in acute myeloid leukaemia from October 2016 to September 2017. During this period, we did one hundred flow cytometry of acute leukaemia patients and among them we found 48 acute myeloid Leukaemia (AML) who were included in this study. Result: Mean age of patients was 35 years (SD­ +14 years; Rang 3 to 50 years) with male: female ratio of 0.92. Four colour flow cytometry was done on fresh bone marrow aspirates and peripheral blood. Among 48 AML patients, aberrant CD expression was observed in 58% cases.  CD5 and cCD79a lymphoid markers were seen to be expressed in 32% cases of AML. Aberrant cCD3 and CD7 were expressed in 29% and 25% cases respectively and aberrant CD10, CD19, cCD22 were expressed in 11%, 3%, 3% cases acute myeloid leukaemia patients respectively. Conclusion: Aberrant CD antigen expression is not uncommon in AML patients of Bangladeshi population that may adversely affect the treatment outcome of the disease.

Monocyte as an Emerging Tool for Targeted Drug Delivery: A Review

2019 ◽  
Vol 24 (44) ◽  
pp. 5296-5312 ◽  
Author(s):  
Fakhara Sabir ◽  
Rai K. Farooq ◽  
Asim.ur.Rehman ◽  
Naveed Ahmed
Keyword(s):  
Drug Delivery ◽  
Targeted Drug Delivery ◽  
The Body ◽  
Carrier System ◽  
Bone Marrow Precursor ◽  
Extensive Introduction ◽  
Cancer Inflammation ◽  
Targeted Drug ◽  
Cluster Of Differentiation

Monocytes are leading component of the mononuclear phagocytic system that play a key role in phagocytosis and removal of several kinds of microbes from the body. Monocytes are bone marrow precursor cells that stay in the blood for a few days and migrate towards tissues where they differentiate into macrophages. Monocytes can be used as a carrier for delivery of active agents into tissues, where other carriers have no significant access. Targeting monocytes is possible both through passive and active targeting, the former one is simply achieved by enhanced permeation and retention effect while the later one by attachment of ligands on the surface of the lipid-based particulate system. Monocytes have many receptors e.g., mannose, scavenger, integrins, cluster of differentiation 14 (CD14) and cluster of differentiation 36 (CD36). The ligands used against these receptors are peptides, lectins, antibodies, glycolipids, and glycoproteins. This review encloses extensive introduction of monocytes as a suitable carrier system for drug delivery, the design of lipid-based carrier system, possible ways for delivery of therapeutics to monocytes, and the role of monocytes in the treatment of life compromising diseases such as cancer, inflammation, stroke, etc.

scholarly journals Pro-Inflammatory Effect of Gliadins and Glutenins Extracted from Different Wheat Cultivars on an In Vitro 3D Intestinal Epithelium Model

2020 ◽  
Vol 22 (1) ◽  
pp. 172
Author(s):  
Francesca Truzzi ◽  
Camilla Tibaldi ◽  
Anne Whittaker ◽  
Silvia Dilloo ◽  
Enzo Spisni ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Innate Immune Responses ◽  
Wheat Proteins ◽  
Monocyte Migration ◽  
Junction Protein ◽  
Modern Wheat ◽  
In Vitro Effects ◽  
Landrace Wheat ◽  
Cluster Of Differentiation

There is a need to assess the relationship between improved rheological properties and the immunogenic potential of wheat proteins. The present study aimed to investigate the in vitro effects of total protein extracts from three modern and two landrace Triticum aestivum commercial flour mixes, with significant differences in gluten strength (GS), on cell lines. Cytotoxicity and innate immune responses induced by wheat proteins were investigated using Caco-2 monocultures, two dimensional (2D) Caco-2/U937 co-cultures, and three dimensional (3D) co-cultures simulating the intestinal mucosa with Caco-2 epithelial cells situated above an extra-cellular matrix containing U937 monocytes and L929 fibroblasts. Modern wheat proteins, with increased GS, significantly reduced Caco-2 cell proliferation and vitality in monoculture and 2D co-cultures than landrace proteins. Modern wheat proteins also augmented Caco-2 monolayer disruption and tight junction protein, occludin, redistribution in 3D co-cultures. Release of interleukin-8 into the cell medium and increased U937 monocyte migration in both 2D and 3D co-cultures were similarly apparent. Immuno-activation of migrating U937 cells was evidenced from cluster of differentiation 14 (CD14) staining and CD11b-related differentiation into macrophages. The modern wheat proteins, with gluten polymorphism relatedness and increased GS, were shown to be more cytotoxic and immunogenic than the landrace wheat proteins.

scholarly journals Identification of cluster of differentiation molecule-associated microRNAs as potential therapeutic targets for gastrointestinal cancer immunotherapy

2021 ◽  
pp. 172460082110054
Author(s):  
Hanyu Zhang ◽  
Mingxing Li ◽  
Parham Jabbarzadeh Kaboli ◽  
Huijiao Ji ◽  
Fukuan Du ◽  
...  
Keyword(s):  
Cancer Immunotherapy ◽  
Cancer Patients ◽  
Gastrointestinal Cancer ◽  
Therapeutic Targets ◽  
Microrna Expression ◽  
The Cancer Genome Atlas ◽  
Gastrointestinal Cancers ◽  
Almost All ◽  
Cluster Of Differentiation

Background: Cluster of differentiation molecules are markers of immune cells that have been identified as a potential immunotherapeutic target for cancer treatment. MicroRNAs are small non-coding RNAs that act as tumor suppressors or oncogenes whose importance in diagnosis, prognosis, and treatment of gastric and colorectal cancers has been widely reported. However, their association with cluster of differentiation molecules in gastrointestinal cancers has not been well studied. Therefore, our study aimed to analyze the relationship between microRNAs and cluster of differentiation molecules in gastrointestinal cancers, and to identify cluster of differentiation molecule-associated microRNAs as prognostic biomarkers for gastrointestinal cancer patients. Methods: Targetscan, Starbase, DIANA microT, and miRDB were used to investigate microRNA profiles that might be correlated with cluster of differentiation molecules in gastrointestinal cancers. Moreover, The Cancer Genome Atlas data analysis was used to investigate the association between cluster of differentiation molecules and microRNA expression in patients with gastric, colon, rectal, pancreatic, and esophageal cancers. The Kaplan–Meier plotter was used to identify the association between overall survival and cluster of differentiation molecule-associated microRNA expression in gastrointestinal cancer patients. Results: miR-200a, miR-559, and miR-1236 were negatively associated with CD86, CD81, and CD160, respectively, in almost all types of gastrointestinal cancers, which were further verified in the in vitro studies by transfecting microRNA mimics in gastric cancer, colon cancer, pancreatic, and esophageal cell lines. Conclusion: Our study showed that miR-200a, miR-1236, and miR-559 are identified as cluster of differentiation-associated microRNAs in gastrointestinal cancers, providing a novel perspective to identify new therapeutic targets for cancer immunotherapy in gastrointestinal cancer patients.

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