Ameliorative role of ethyl-acetate fraction of methanolic leaf extract of Camellia sinensis (green tea) on streptozotocin-induced diabetes linked testicular hypofunction in albino rat: A dose-dependent biochemical and genomic transection study

2017 ◽  
Vol 14 (4) ◽  
Author(s):  
Barnali Das ◽  
Baisakhi Biswas ◽  
Abhinandan Ghosh ◽  
Bhabani Prasad Pakhira ◽  
Debidas Ghosh
Keyword(s):  
Ethyl Acetate ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Toxicity Assessment ◽  
Diabetic Group ◽  
Diabetic Rat ◽  
Albino Rat ◽  
Dose Dependent Fashion ◽  
Dose Dependent

AbstractBackgroundThe present investigation focuses the diabetes-induced testicular hypofunction and its possible correction by the effective dose of ethyl-acetate fraction of methanolic extract ofMethodsThe androgenic, spermiological, oxidative stress and apoptosis sensors along with testicular genomic sensors were evaluated in a dose-dependent fashion (50 mg or 100 mg or 200 mg/kg body weight). Activities of hepatic transaminases for toxicity assessment were also measured.ResultsIncreased level of fasting blood glucose, testicular cholesterol, seminal vesicular fructose along with a low count, motility and viability of epididymal sperm, low activities of testicular Δ5, 3β-hydroxy steroid dehydrogenase (HSD), 17β-HSD, testicular antioxidant enzymes (catalase and superoxide dismutase) and low plasma level of testosterone were noted in diabetic rat in respect to the control. After oral administration of said fraction to diabetic rat, levels of above sensors were resettled toward the control. A significant decrease in the number of different generations of germ cells at the stage VII of spermatogenesis in diabetic rat was noted which were recovered significantly toward the control in the fraction-treated diabetic group. It was supported by the correction in gene expression of testicular Δ5, 3β- HSD, 17β- HSD, Bcl-2 and Bax in the fraction-treated diabetic group.ConclusionsThe threshold dose of ethyl-acetate fraction of methanolic extract of

scholarly journals Evaluation of antidiabetic and antioxidative efficacy of ethyl acetate fraction of methanolic extract of Camellia sinensis (green tea) leaves in streptozotocin induced diabetic albino rat

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Baisakhi Biswas ◽  
Barnali Das ◽  
Kishalay Jana ◽  
Debidas Ghosh
Keyword(s):  
Ethyl Acetate ◽  
Green Tea ◽  
Camellia Sinensis ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rat ◽  
Hepatic Tissue ◽  
Tea Leaves ◽  
Green Tea Leaves

<p><strong>Objective:</strong> To investigate the antidiabetic and antioxidative potentiality of ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves in streptozotocin induced diabetic rat. <strong>Methods:</strong> Streptozotocin induced diabetic state was confirmed by increased level of fasting blood glucose, decreased level of serum insulin along with inhibition in carbohydrate metabolomics. Oxidative stress was assessed by measuring antioxidative enzyme activities of hepatic and skeleto-muscular tissue. Hepatic <em>Hexokinase-I</em>, pro-apoptotic <em>Bax</em> and anti-apoptotic <em>Bcl-2</em> gene expression patterns were noted by qRT-PCR technique. <strong>Results:</strong> After treatment with ethyl-acetate fraction of methanolic extract of <em>Camellia sinensis</em> (Green tea) leaves at a dose of 100 mg/kg body weight/day to diabetic rats for 28 days, a significant (p &lt; 0.05) recovery was noted in fasting blood glucose level, serum insulin level along with activities of carbohydrate metabolic enzymes in hepatic tissue in respect to the vehicle treated diabetic group. This fraction also resulted a significant (p &lt; 0.05) recovery in the activities of antioxidative enzymes in hepatic and skeleto - mascular tissue. In streptozotocin induced diabetic rat the low level of expression of <em>Hexokinase-I</em>, anti-apoptotic <em>Bcl-2</em> and high level of expression of pro-apoptotic <em>Bax</em> gene were observed in hepatic tissue in respect to vehicle treated control. There were recovered significantly after the treatment with the said fraction. <strong>Conclusion: </strong>From the results, it may be concluded that ethyl-acetate fraction of methanolic extract of leaves of <em>C. sinensis</em> has a promising anti-diabetic and antioxidative activities for the management of streptozotocin induced diabetic state.</p>

Antidiabetic and antioxidative properties of the hydro-methanolic extract (60:40) of rhizomes of Curcuma amada roxb. (Zingiberaceae) in streptozotocin-induced diabetic male albino rat: a dose-dependent study through biochemical and genomic approaches

2019 ◽  
Vol 16 (4) ◽  
Author(s):  
Dipanwita Mitra ◽  
Riya Sarkar ◽  
Debidas Ghosh
Keyword(s):  
Body Weight ◽  
Methanolic Extract ◽  
Fasting Blood Glucose ◽  
Diabetic Rats ◽  
Diabetic Group ◽  
Dependent Manner ◽  
Male Adult ◽  
Curcuma Amada ◽  
Corrective Effect ◽  
Dose Dependent

Abstract Background Curcuma amada is the most popular traditional medicine in India for the treatment of diabetes. The present study aimed to focus the antidiabetic and antioxidative activity of C. amada through the analysis of biochemical and genomic levels in a dose-dependent manner in streptozotocin-induced male adult rat. Method Streptozotocin-induced diabetic rats were administered orally with hydro-methanolic extract of C. amada at the dose of 10, 20, 40 and 80 mg/100 g body weight of rats for 28 days. The antidiabetic and antioxidative efficacy of the extract on glycemic, enzymatic, genomic and histological sensors along with toxicity study was investigated. Results The result showed a significant antidiabetic and antioxidative effect of the extract at dose-dependent manner. The significant recovery of fasting blood glucose level, serum insulin, activity of carbohydrate metabolic enzymes and antioxidative enzymes in extract-treated diabetic group as compared to untreated diabetic group were noted. After the extract treatment, the size of pancreatic islet and cell population densities were significantly increased. Activities of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase in liver were significantly recovered along with the correction of Bax and Bcl-2 gene expression in hepatic tissue after the extract treatment in diabetic rats in respect to untreated diabetic group. Out of all the doses, the significant effects were noted at the dose of 20 mg/100 g body weight which has been considered as threshold dose in the concern. Conclusion It may be concluded that the significant and corrective effect in most of the sensors was noted at the minimum dose of 20 mg/100 g body weight of hydro-methanolic extract of C. amada without producing any toxicity.

scholarly journals Marine Natural Products, IX A New Halogenated Sesquiterpene Pinnatifidone from the Red Alga Laurencia pinnatifida

1988 ◽  
Vol 43 (10) ◽  
pp. 1347-1350 ◽  
Author(s):  
Shaheen Bano ◽  
Mohammad Shaiq Ali ◽  
Viqar Uddin Ahmad
Keyword(s):  
Natural Products ◽  
Ethyl Acetate ◽  
Marine Natural Products ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Spectroscopic Studies ◽  
Red Alga

Abstract From the ethyl acetate fraction of methanolic extract of red alga, L.pinnatifida, a new halogenated sesquiterpene named as pinnatifidone [1] has been isolated and the structure of this compound has been elucidated with the help of intensive spectroscopic studies.

scholarly journals The ethyl acetate fraction of a methanolic extract of unripe noni (Morinda citrifolia Linn.) fruit exhibits a biphasic effect on the dopaminergic system in mice

2017 ◽  
Vol 66 (3) ◽  
pp. 283-291 ◽  
Author(s):  
Vijayapandi Pandy ◽  
Megala Narasingam ◽  
Kamini Vijeepallam ◽  
Syam Mohan ◽  
Vasudevan Mani ◽  
...  
Keyword(s):  
Ethyl Acetate ◽  
Dopaminergic System ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Morinda Citrifolia ◽  
Biphasic Effect

scholarly journals Dose-Dependent Effects of Recombinant Human Interleukin-6 on Glucose Regulation

1997 ◽  
Vol 82 (12) ◽  
pp. 4167-4170 ◽  
Author(s):  
Constantine Tsigos ◽  
Dimitris A. Papanicolaou ◽  
Ioannis Kyrou ◽  
Ruby Defensor ◽  
Constantine S. Mitsiadis ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Interleukin 6 ◽  
Peak Plasma ◽  
Fasting Blood Glucose ◽  
Peripheral Resistance ◽  
C Peptide ◽  
Inflammatory Stress ◽  
Glucose Levels ◽  
Dose Dependent Fashion ◽  
Dose Dependent

Inflammatory cytokines have metabolic actions that probably contribute to the general adaptation of the organism during infectious or inflammatory stress. To examine the effects of interleukin 6 (IL-6), the main circulating cytokine, on glucose metabolism in man, we performed dose-response studies of recombinant human IL-6 in normal volunteers. Increasing single doses of IL-6 (0.1, 0.3, 1.0, 3.0, and 10.0 mg/Kg BW) were injected sc in 15 healthy male volunteers (3 in each dose) after a 12-h fast. All IL-6 doses were tolerated well and produced no significant adverse effects. We measured the circulating levels of glucose, insulin, C-peptide, and glucagon at baseline and half-hourly over 4 h after the IL-6 injection. Mean peak plasma levels of IL-6 were achieved between 120 and 240 min and were 8, 22, 65, 290, and 4050 pg/mL, respectively, for the 5 doses. After administration of the 2 smaller IL-6 doses, we observed no significant changes in plasma glucose levels, which, because of continued fasting, decreased slightly over time. By 60 min after the 3 higher IL-6 doses, however, the decline in fasting blood glucose was arrested, and glucose levels increased in a dose-dependent fashion. The concurrent levels of plasma insulin and C-peptide were not affected by any IL-6 dose. In contrast, IL-6 caused significant increases in plasma glucagon levels, which peaked between 120 and 150 min after the IL-6 injection. In conclusion, sc IL-6 administration induced dose-dependent increases in fasting blood glucose, probably by stimulating glucagon release and other counteregulatory hormones and/or by inducing peripheral resistance to insulin action.

scholarly journals Methanolic extract of Potentilla fulgens root and its ethyl-acetate fraction delays the process of carcinogenesis in mice

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Buddha Ganguly ◽  
Alka Chaudhary ◽  
Hughbert Dakhar ◽  
Inder Pal Singh ◽  
Anupam Chatterjee
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Genetic Alterations ◽  
Gastric Carcinogenesis ◽  
Check Point ◽  
Over Expression ◽  
North Eastern ◽  
Ad Libitum

AbstractPeople of north-eastern states of India consume raw areca-nut (RAN) and lime which could lead to oral, esophageal and gastric cancers. However, the incidence of these cancers are significantly lesser in those who consume pieces of Potentilla fulgens root along with RAN. Since evaluation of anticancer role, if any, of P. fulgens on RAN-mediated genetic alterations in human is difficult because of other compounding factors, this study was undertaken in mice to focus on gastric carcinogenesis since ad libitum administration of RAN extract with lime in drinking water induced stomach cancer due to greater exposure of its lining. A total of 160 mice were used at different time points and either methanol extract of P. fulgens roots (PRE) or mixture of four compounds of ethyl-acetate fraction (EA-mixture) was mixed with mice feed. Histological studies revealed that RAN + lime induced cancer in all the mice and interestingly only 20% developed cancer when PRE/EA-mixture was provided along with RAN + lime. Higher frequency of precocious anaphase and over expression of p53 and Securin genes were significantly reduced by PRE/EA-mixture. Thus PRE/EA-mixture mitigates the RAN-induced tumor-initiating process in stomach by maintaining expression of tumor suppressor and check-point genes under control.

scholarly journals Effect of Root Extract Fractions of Kyllinga triceps Rottb on Streptozotocin Induced Diabetic Rats

1970 ◽  
Vol 4 (1) ◽  
pp. 25-30
Author(s):  
Swaroopa Rani Vanapatla ◽  
G Krishna Mohan ◽  
B Ravi Kumar
Keyword(s):  
Blood Glucose ◽  
Blood Glucose Level ◽  
Ethyl Acetate ◽  
Glucose Level ◽  
Fasting Blood Glucose ◽  
Ethyl Acetate Fraction ◽  
Diabetic Rats ◽  
Root Extract ◽  
Neonatal Rats ◽  
Acute Study

The present study was aimed to evaluate the root extract fractions of Kyllinga triceps (KT) for their antidiabetic potential on streptozotocin induced diabetes in neonatal rats. Diabetes was induced by a single intraperitoneal injection of Streptozotocin (90mg/kg) to 48±2h old neonatal rats. Effect of root extract fractions (toluene, ethyl acetate, 1- butanol at 50 &100 mg/kg.) were tested for their antihyperglycemic activity by measuring their fasting blood glucose level in diabetic rats at 0,2,4,6,8,12 & 24 h after the treatment. In sub acute study ethyl acetate fraction of KT (EAKT) was administered daily to diabetic rats orally at a dose of 100mg/kg for 28 days. Body weight of the animals and blood glucose level were observed at weekly interval during the study. Cholesterol, triglycerides, insulin, SGPT, ALP, creatinine and total proteins level in serum were also estimated at the initial and after 28 days of the treatment. As the preliminary investigation conducted in our lab on methanolic extract of the roots of KT had showed significant oral glucose tolerance with 200 mg/kg in normal rats. Oral administration of fractions of the plant significantly reduced the fasting blood glucose level in diabetic rats. Among the fractions, EAKT was found to be more effective. Further, in sub-acute study, EAKT, showed a significant anti diabetic activity by reversal of the altered afore said serum biochemical parameters. The results of the study are substantiating the traditional claim of the roots of Kyllinga triceps in the treatment of diabetes with a scope for development of antidiabetic herbal drug from EAKT.   Key words: Antidiabetic activity; Kyllinga triceps; Ethyl acetate fraction; Streptozotocin. DOI: http://dx.doi.org/10.3329/sjps.v4i1.8863 SJPS 2011; 4(1): 25-30

Evaluation of Cytotoxic Compounds from Calligonum comosum L. Growing in Egypt

2007 ◽  
Vol 62 (9-10) ◽  
pp. 656-660 ◽  
Author(s):  
Farid A. Badria ◽  
Madiha Ameen ◽  
Mohamed R. Akl
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Brine Shrimp ◽  
Methylene Chloride ◽  
Methanolic Extract ◽  
Ethyl Acetate Fraction ◽  
Desert Plant ◽  
Ehrlich Ascites ◽  
Cytotoxic Compounds ◽  
Egyptian Desert

Calligonum comosum (Polygonaceae), an Egyptian desert plant, was extracted and fractionated using petroleum ether, methylene chloride, and ethyl acetate. The total methanolic extract and other fractions were tested for their anticancer activity using Ehrlich ascites, brine shrimp and antioxidant assays. Ethyl acetate fraction proved to be the most active in all assays. Eight compounds were isolated, purified, and identified from this fraction as (+)- catechin (1), dehydrodicatechin A (2), kaempferol-3-O-rhamnopyranoside (3), quercitrin (quercetin-3-O-rhamnopyranoside) (4), β-sitosterol-3-O-glucoside (5), isoquercitrin (quercetin- 3-O-glucopyranoside) (6), kaempferol-3-O-glucuronide (7), and mequilianin (quercetin-3- O-glucuronide) (8). All isolated compounds were tested for their cytotoxicity and antioxidant activity. Compound 2 showed the best cytotoxic and antioxidant activity.

scholarly journals Ethyl Acetate Fraction of Teucrium Polium Extract Abolishes Human Umbilical Vein Endothelial Cells (HUVEC) Tubulogenesis in Collagen Bed through Suppression of Cell Proliferation/VEGF Secretion

2019 ◽  
Author(s):  
Vahide Askari ◽  
Somayeh Shamlou ◽  
Ali Mostafaie ◽  
Sara Khaleqi
Keyword(s):  
Endothelial Cells ◽  
Ethyl Acetate ◽  
Umbilical Vein ◽  
Ethyl Acetate Fraction ◽  
Dependent Manner ◽  
Angiogenic Effect ◽  
Teucrium Polium ◽  
Umbilical Vein Endothelial Cells ◽  
Dose Dependent

Angiogenesis has essential role in growth and metastasis of tumors. Development of therapies aimed to suppress angiogenesis using medicinal plants is one of the effective approaches for prevention/treatment of cancer. The current study was performed to investigate in vitro anti-angiogenic effect of Teucrium Polium (TP) extract and its fractions. The aerial part of Teucrium Polium was powdered and extracted with 50% ethanol. The extract was fractionated in to aqueous (AQ), n-butanol (BU), ethyl acetate (EA) and n-hexane (HE) fractions. Anti-angiogenic effect of TP was examined on human umbilical vein endothelial cells (HUVECs) in three-dimensional collagen matrix. The endothelial cells form capillary-like branches that can be visualized using phase contrast microscope and the number of tube-like structures can be quantified as a measure of in vitro angiogenesis. Furthermore, anti-proliferative and vascular endothelial growth factor(VEGF )suppressive effect of TP as important factors in the process of angiogenesis were assessed using3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT)and quantitative ELISA, respectively. Based on our findings, among the TP fractions, EA fraction showed the highest inhibitory activity on angiogenesis. This fraction with IC50: 68 µg/mL, inhibited angiogenesis at 60 µg/mL. The crude extract and other fractions of TP inhibited angiogenesis in a dose-dependent manner at doses higher concentrations than EA fraction, significantly.TP extract and EA fraction were able to inhibit proliferation of HUVEC and inhibited VEGF secretion in a dose dependent manner. The ethyl acetate fraction at 60 µg/ml inhibited VEGF secretion perfectly. Our data indicated that ethyl acetate fraction of Teucrium Polium could be a potential candidate for the prevention of angiogenesis in cancer and other related disorders. However, this suggestion needs more quantitative and in vivo investigations for confirmation.

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