Antimicrobial activities of methanolic extract of Carissa opaca roots and its fractions and compounds isolated from the most active ethyl acetate fraction

Abstract From the ethyl acetate fraction of methanolic extract of red alga, L.pinnatifida, a new halogenated sesquiterpene named as pinnatifidone [1] has been isolated and the structure of this compound has been elucidated with the help of intensive spectroscopic studies.

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The ethyl acetate fraction of a methanolic extract of unripe noni (Morinda citrifolia Linn.) fruit exhibits a biphasic effect on the dopaminergic system in mice

Experimental Animals ◽

10.1538/expanim.16-0105 ◽

2017 ◽

Vol 66 (3) ◽

pp. 283-291 ◽

Cited By ~ 3

Author(s):

Vijayapandi Pandy ◽

Megala Narasingam ◽

Kamini Vijeepallam ◽

Syam Mohan ◽

Vasudevan Mani ◽

...

Keyword(s):

Ethyl Acetate ◽

Dopaminergic System ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Morinda Citrifolia ◽

Biphasic Effect

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Study and evaluation of antimicrobial activity and antioxidant capacity of dry extract and fractions of leaves of Raphanus sativus var. oleiferus Metzg.

Bioscience Journal ◽

10.14393/bj-v36n2a2020-41848 ◽

2020 ◽

Vol 36 (2) ◽

Cited By ~ 1

Author(s):

Ana Flávia da Silva ◽

Marisa de Oliveira Lopes ◽

Cláudio Daniel Cerdeira ◽

Ingridy Simone Ribeiro ◽

Isael Aparecido Rosa ◽

...

Keyword(s):

Antimicrobial Activity ◽

Antioxidant Activity ◽

Minimum Inhibitory Concentration ◽

Ethyl Acetate ◽

Raphanus Sativus ◽

Inhibitory Concentration ◽

Ethyl Acetate Fraction ◽

Antimicrobial Activities ◽

Dry Extract ◽

Antioxidant And Antimicrobial Activities

The radish (Raphanus sativus L.) is a vegetable of the Brassicaceae family cultivated worldwide and has several medicinal properties. Its biological activities are related to various secondary metabolites present in the species, especially phenolics. Thus, the objectives of this study were the chemical analysis and evaluation of the antioxidant and antimicrobial activities of the dry extract and fractions of the fodder turnip leaves (R. sativus var. oleiferus Metzg.). Samples were analyzed by mass spectrometry and the antioxidant activity was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical method and the reducing power method. Antimicrobial activity was determined by the agar diffusion and microdilution methods. The total phenols were concentrated in the butanol fraction (121.27 mg GAE/g) and the flavonoids were concentrated in the ethyl acetate fraction (98.02 mg EQ/g). The ethyl acetate fraction showed the best antioxidants results, with 83.45% of free radical scavenging and 11.34% of ferric ions reduction. The analysis of antimicrobial activity showed that the dry extract had the highest average zone of inhibition against Bacillus subtilis (18.67 mm). Smaller values of the minimum inhibitory concentration for Micrococcus luteus were, and the ethyl acetate fraction showed a lower minimum inhibitory concentration (0.1 mg/ml) for that microorganism. There was a strong correlation between the antioxidant activity and the content of phenols and flavonoids. The results showed the potential antioxidant and antimicrobial activities of this extract with the ethyl acetate fraction being most promising for further studies.

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Methanolic extract of Potentilla fulgens root and its ethyl-acetate fraction delays the process of carcinogenesis in mice

Scientific Reports ◽

10.1038/s41598-019-53747-5 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Buddha Ganguly ◽

Alka Chaudhary ◽

Hughbert Dakhar ◽

Inder Pal Singh ◽

Anupam Chatterjee

Keyword(s):

Ethyl Acetate ◽

Methanol Extract ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Genetic Alterations ◽

Gastric Carcinogenesis ◽

Check Point ◽

Over Expression ◽

North Eastern ◽

Ad Libitum

AbstractPeople of north-eastern states of India consume raw areca-nut (RAN) and lime which could lead to oral, esophageal and gastric cancers. However, the incidence of these cancers are significantly lesser in those who consume pieces of Potentilla fulgens root along with RAN. Since evaluation of anticancer role, if any, of P. fulgens on RAN-mediated genetic alterations in human is difficult because of other compounding factors, this study was undertaken in mice to focus on gastric carcinogenesis since ad libitum administration of RAN extract with lime in drinking water induced stomach cancer due to greater exposure of its lining. A total of 160 mice were used at different time points and either methanol extract of P. fulgens roots (PRE) or mixture of four compounds of ethyl-acetate fraction (EA-mixture) was mixed with mice feed. Histological studies revealed that RAN + lime induced cancer in all the mice and interestingly only 20% developed cancer when PRE/EA-mixture was provided along with RAN + lime. Higher frequency of precocious anaphase and over expression of p53 and Securin genes were significantly reduced by PRE/EA-mixture. Thus PRE/EA-mixture mitigates the RAN-induced tumor-initiating process in stomach by maintaining expression of tumor suppressor and check-point genes under control.

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PHYTOCHEMICAL AND ANTIMICROBIAL ACTIVITY OF THE LEAVES OF LANNEA KERSTINGII ENGL & K. KRAUSE (ANACADIACEAE)

Journal of Health and Allied Sciences NU ◽

10.1055/s-0040-1703823 ◽

2014 ◽

Vol 04 (04) ◽

pp. 004-009

Author(s):

Njinga NS ◽

Sule MI ◽

Pateh UU ◽

Hassan HS ◽

Ahmad MM ◽

...

Keyword(s):

Antimicrobial Activity ◽

Ethyl Acetate ◽

Petroleum Ether ◽

Methanol Extract ◽

Petroleum Ether Extract ◽

Ethyl Acetate Fraction ◽

Antimicrobial Activities ◽

Chloroform Fraction ◽

Ether Extract ◽

Minimum Fungicidal Concentration

AbstractThe phytochemical and antimicrobial activity of the petroleum ether and crude methanol extracts, chloroform and ethyl acetate fractions of the leaves of Lannea kerstingii were investigated. Phytochemical screening revealed the presence of steroids and triterpenes in the petroleum ether extract, steroid, triterpene, flavonoids and tannins in both crude methanol extract and chloroform fraction while the ethyl acetate fraction contained only flavonoids and tannins. The extracts exhibited antimicrobial activities with zones of inhibition ranging from 17.00 to 21.03, 20.10 to 25.24, 25.32 to 34.02 and 22.28 to 27.20 mm for petroleum ether extract, methanol extract, chloroform and ethyl acetate fractions respectively. The minimum inhibitory concentration was between 5 and 10mg/ml, 5mg/ml for the petroleum ether and methanol extract respectively, and between 2.5 and 5 mg/ml, 5mg/ml for the acetate fractions. The minimum bactericidal concentration for all the extracts was 40mg/ml respectively except for chloroform fraction which ranged from 20 to 40mg/ml. The minimum fungicidal concentration for all the extracts was found to be 40mg/ml respectively. This result indicates the broad spectrum antimicrobial potential of L. Kerstingii and justifies the use of this plant in traditional medicine.

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In Vitro Antioxidant and Antimicrobial Activities of Methanol Leaf Extract and Fractions of Afzelia bellaHarms (Fabaceae)

Ethiopian Pharmaceutical Journal ◽

10.4314/epj.v36i1.3 ◽

2020 ◽

Vol 36 (1) ◽

pp. 19-30

Author(s):

Josephine Ofeimun ◽

James Afolabi ◽

Ejiro Dowe ◽

Osayemwenre Erhauyi ◽

Enitome Bafor ◽

...

Keyword(s):

Antimicrobial Activity ◽

Bacillus Subtilis ◽

Ethyl Acetate ◽

Leaf Extract ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Antimicrobial Activities ◽

Alcoholic Extract ◽

Antioxidant Power ◽

Antioxidant And Antimicrobial Activities

Afzelia bella Harms (Fabaceae), a plant widely distributed in Africa, is used in traditional medicine for varied disease conditions including the treatment of topical skin infections. The present study investigated the antimicrobial and antioxidant activities of the methanol extract and various solvent fractions of the leaves of the plant. The methanol leaf extract was partitioned to yield petroleum ether, chloroform, ethyl acetate and residual aqueous fractions. Total phenol and flavonoid contents, radical scavenging activity and ferric reduction antioxidant power (FRAP) were determined by spectrophotometry, while antimicrobial activity and minimum inhibitory concentration (MIC) of the extract and fractions were determined using agar-well diffusion and agar dilution methods, respectively against clinical bacterial isolates of Bacillus subtilis, Escherishia coli, Klebsiella pneumoniae, Staphylococcus aureus, and fungi, Aspergillus flavus, Candida parapsilosis, Microsporium audiounii. Bacillus subtilis was the most susceptible among the bacterial strains tested, while Microsporium audiounii was the most susceptible fungus. The alcoholic extract and all solvent fractions demonstrated a concentration dependent antimicrobial activity with inhibition zone diameter range of 7.5 to 35.0 mm. MIC ranged from 0.1 - 8 mg/ml and activity was highest in ethyl acetate fraction with MIC of 0.1 mg/ml. FRAP ranged from 0.161 - 0.319 mmol Fe2+/g extract and was highest in the ethyl acetate fraction. These results give an indication that A. bella leaf has high antioxidant and antimicrobial activities and support the folkloric claim of the therapeutic potential of the plant. Keywords: antioxidant, antimicrobial, ethnomedicine, Afzelia bella, Fabaceae

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Evaluation of Cytotoxic Compounds from Calligonum comosum L. Growing in Egypt

Zeitschrift für Naturforschung C ◽

10.1515/znc-2007-9-1005 ◽

2007 ◽

Vol 62 (9-10) ◽

pp. 656-660 ◽

Cited By ~ 46

Author(s):

Farid A. Badria ◽

Madiha Ameen ◽

Mohamed R. Akl

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Brine Shrimp ◽

Methylene Chloride ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

Desert Plant ◽

Ehrlich Ascites ◽

Cytotoxic Compounds ◽

Egyptian Desert

Calligonum comosum (Polygonaceae), an Egyptian desert plant, was extracted and fractionated using petroleum ether, methylene chloride, and ethyl acetate. The total methanolic extract and other fractions were tested for their anticancer activity using Ehrlich ascites, brine shrimp and antioxidant assays. Ethyl acetate fraction proved to be the most active in all assays. Eight compounds were isolated, purified, and identified from this fraction as (+)- catechin (1), dehydrodicatechin A (2), kaempferol-3-O-rhamnopyranoside (3), quercitrin (quercetin-3-O-rhamnopyranoside) (4), β-sitosterol-3-O-glucoside (5), isoquercitrin (quercetin- 3-O-glucopyranoside) (6), kaempferol-3-O-glucuronide (7), and mequilianin (quercetin-3- O-glucuronide) (8). All isolated compounds were tested for their cytotoxicity and antioxidant activity. Compound 2 showed the best cytotoxic and antioxidant activity.

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STUDY ON ANTI-MICROBIAL PROPERTIES OF Enicosanthum membranifolium SINCLAIR AND Enicosanthum cupulare (KING) AIRY-SHAW

Jurnal Riset Kimia ◽

10.25077/jrk.v3i1.75 ◽

2015 ◽

Vol 3 (1) ◽

pp. 62

Author(s):

Mai Efdi ◽

Mamoru Koketsu ◽

Kunitomo Watanabe ◽

Nurainas

Keyword(s):

Ethyl Acetate ◽

Inhibitory Concentration ◽

Ethyl Acetate Fraction ◽

Antimicrobial Activities ◽

Dilution Method ◽

Agar Dilution Method ◽

Gram Negative Bacteria ◽

Bacterial Strains ◽

Microbial Properties ◽

Agar Dilution

ABSTRACT n-Hexane and ethyl acetate fractions of methanol extracts of two species of Annonaceae, Enicosanthum membranifolium Sinclair and Enicosanthum cupulare (King) Airy-Shaw, were screened for antimicrobial activity against eighteen bacterial strains using agar dilution method. The ethyl acetate fraction of the two Annonaceae plants showed higher antimicrobial activities than the n-hexane fraction. The extracts of the plants tested were significantly more active against gram-positive with minimum inhibitory concentration (MICs) ranging from 0.0625 to 4 mg/mL than against gram negative bacteria (MICs >4 mg/mL). Keywords: Antibacterial activity; Enicosanthum membranifolium Sinclair; Enicosanthum cupulare (King) Airy-Shaw

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Determination of Antioxidant Activity and Phenolic Compounds of Methanolic Extract of Java Plum (Syzygium cumini Linn. (Skeel) Seed

Indonesian Food and Nutrition Progress ◽

10.22146/ifnp.24279 ◽

2017 ◽

Vol 14 (1) ◽

pp. 9 ◽

Cited By ~ 2

Author(s):

Rohadi Rohadi ◽

Umar Santoso ◽

Sri Raharjo ◽

Iip Izul Falah

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanolic Extract ◽

Radical Scavenging ◽

Ethyl Acetate Fraction ◽

Polar Fraction ◽

Acid Oxidation ◽

Syzygium Cumini ◽

Compound Identification ◽

Antioxidant Power

Methanolic extract of Java Plum (Syzygium cumini L. (Skeel) seed (MEJS) is potential source of natural antioxidant. As indicated by several in vitro measurements, the extract had strong DPPH (1,1 diphenyl, 2–picryl hydrazyl) and ABTS (2,2-azinobis, 3-ethylbenzothiazoline-6-sulphonate) radical scavenging activity, strong Ferric Reducing Antioxidant Power (FRAP) and moderate inhibition activity of linoleic acid oxidation. This study aimed to determine antioxidant activity and phenolic compound of Java Plum seed (Syzygium cumini L. (Skeel) methanolic extract fractions. Phenolics compound identification using Thin Layer Chromatography (TLC) showed that all fractions (polar, semi polar and hydrolyzed semi polar fraction) contained Gallic acid, Tannic acid and flavonol’s Rutin. HPLC-DAD analysis showed that its polar fraction contained 25 ppm flavonol’s Quercetine and 55181 ppm flavonol’s (+)- Catechin, ethyl acetate fraction contained 54 ppm flavonol’s Rutin and 528 ppm (+)- Catechine, while hydrolyzed ethyl acetate fraction contained 404 ppm Rutin and 28692 ppm (+)- Catechine.

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Secondary Metabolites of Ponderosa Lemon (Citrus pyriformis) and their Antioxidant, Anti-Inflammatory, and Cytotoxic Activities

Zeitschrift für Naturforschung C ◽

10.1515/znc-2011-7-810 ◽

2011 ◽

Vol 66 (7-8) ◽

pp. 385-393 ◽

Cited By ~ 5

Author(s):

Dalia Hamdan ◽

Mahmoud Zaki El-Readi ◽

Ahmad Tahrani ◽

Florian Herrmann ◽

Dorothea Kaufmann ◽

...

Keyword(s):

Ethyl Acetate ◽

Petroleum Ether ◽

Methanolic Extract ◽

Ethyl Acetate Fraction ◽

2D Nmr ◽

Positive Control ◽

Cytotoxic Activities ◽

Fruit Peel ◽

Ether Fraction ◽

Anti Inflammatory

Column chromatography of the dichloromethane fraction from an aqueous methanolic extract of fruit peel of Citrus pyriformis Hassk. (Rutaceae) resulted in the isolation of seven compounds including one coumarin (citropten), two limonoids (limonin and deacetylnomilin), and four sterols (stigmasterol, ergosterol, sitosteryl-3-β-D-glucoside, and sitosteryl-6ʹ- O-acyl-3-β-D-glucoside). From the ethyl acetate fraction naringin, hesperidin, and neohesperidin were isolated. The dichloromethane extract of the defatted seeds contained three additional compounds, nomilin, ichangin, and cholesterol. The isolated compounds were identified by MS (EI, CI, and ESI), 1H, 13C, and 2D-NMR spectral data. The limonoids were determined qualitatively by LC-ESI/MS resulting in the identification of 11 limonoid aglycones. The total methanolic extract of the peel and the petroleum ether, dichloromethane, and ethyl acetate fractions were screened for their antioxidant and anti-inflammatory activities. The ethyl acetate fraction exhibited a significant scavenging activity for DPPH· free radicals (IC50 = 132.3 μg/mL). The petroleum ether fraction inhibited 5-lipoxygenase with IC50 = 30.6 μg/mL indicating potential anti-inflammatory properties. Limonin has a potent cytotoxic effect against COS7 cells [IC50 = (35.0 ± 6.1) μM] compared with acteoside as a positive control [IC50 = (144.5 ± 10.96) μM]

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