Separation and purification of rebaudioside A from extract of Stevia Rebaudiana leaves by macroporous adsorption resins

Abstract The separation and purification of rebaudioside A from Stevia rebaudiana crude extracts (Steviosides) by macroporous resin were optimized by Taguchi orthogonal array (OA) experimental design methodology. This approach was applied to evaluate the influence of five factors (adsorption temperature, desorption time, elution solution ratio, adsorption volume and type of resin) on the rebaudioside A yield. The percentage contribution of each factor was also determined. The results showed that elution solution ratio and adsorption volume made the greatest (59.6%) and the lowest (1.3%) contribution, respectively. The results showed that the Taguchi method is able to model the purification of rebaudioside A process well (R2 > 0.998) and can therefore be applied in future studies conducted in various fields. Adsorption temperature 35°C, desorption time 60min, elution solution ratio 3, adsorption volume 200ml and HPD-400 as resin were the best conditions determined by the Taguchi method.

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SEPARATION OF REBAUDIOSIDE A FROM Stevia rebaudiana EXTRACT USING LOW POLARITY RESIN AB-8

Borneo International Journal of Biotechnology (BIJB) ◽

10.51200/bijb.vi.1546 ◽

2020 ◽

pp. 45

Author(s):

Chong Saw Peng ◽

Norazlina Noordin ◽

Mustapha Akil ◽

Norellia Bahari

Keyword(s):

Ion Exchange ◽

Stevia Rebaudiana ◽

Ion Exchange Chromatography ◽

Exchange Chromatography ◽

Rebaudioside A ◽

Macroporous Resin ◽

Adsorption Volume ◽

Desorption Time ◽

Adsorption Temperature ◽

High Polarity

There are many methods to separate or purify the rebaudioside A compound from Stevia rebaudiana extract. However, the ion-exchange chromatography using macroporous resin is still the most popular among those methods. The separation of rebaudioside A from stevia crude extract by macroporous resin AB-8 was optimised in this adsorption separation study. This approach was applied to evaluate the influence of four factors such as the adsorption temperature, desorption time, elution solution ratio, and adsorption volume on rebaudioside A yield of the purified stevia extract. Theresults showed that the low polarity resin AB-8 is able to separate rebaudioside A from stevia extract with 0.601 in yield compared to the high polarity resin HPD 600 with 0.204 in yield used in Anvari and Khayati study. The best conditions for rebaudioside A separation by macroporous resin AB-8 were at 35°C of adsorption temperature, 30 min of desorption time, elution solution ratio 2:1, and 50 mL of adsorption volume.

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Preparative separation and purification of Rebaudioside A from Stevia rebaudiana Bertoni crude extracts by mixed bed of macroporous adsorption resins

Food Chemistry ◽

10.1016/j.foodchem.2011.10.077 ◽

2012 ◽

Vol 132 (1) ◽

pp. 268-276 ◽

Cited By ~ 29

Author(s):

Jie Li ◽

Zhenbin Chen ◽

Duolong Di

Keyword(s):

Stevia Rebaudiana ◽

Rebaudioside A ◽

Preparative Separation ◽

Separation And Purification ◽

Stevia Rebaudiana Bertoni ◽

Crude Extracts ◽

Macroporous Adsorption Resins

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Preparative Separation and Purification of Rebaudioside A from Steviol Glycosides Using Mixed-Mode Macroporous Adsorption Resins

Journal of Agricultural and Food Chemistry ◽

10.1021/jf2020232 ◽

2011 ◽

Vol 59 (17) ◽

pp. 9629-9636 ◽

Cited By ~ 58

Author(s):

Yongfeng Liu ◽

Duolong Di ◽

Qingqing Bai ◽

Jintian Li ◽

Zhenbin Chen ◽

...

Keyword(s):

Mixed Mode ◽

Steviol Glycosides ◽

Rebaudioside A ◽

Preparative Separation ◽

Separation And Purification ◽

Macroporous Adsorption Resins

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Salt Stress Mitigation via the Foliar Application of Chitosan-Functionalized Selenium and Anatase Titanium Dioxide Nanoparticles in Stevia (Stevia rebaudiana Bertoni)

Molecules ◽

10.3390/molecules26134090 ◽

2021 ◽

Vol 26 (13) ◽

pp. 4090

Author(s):

Morteza Sheikhalipour ◽

Behrooz Esmaielpour ◽

Gholamreza Gohari ◽

Maryam Haghighi ◽

Hessam Jafari ◽

...

Keyword(s):

Titanium Dioxide ◽

Salt Stress ◽

Titanium Dioxide Nanoparticles ◽

Chitosan Nanoparticles ◽

Stevia Rebaudiana ◽

High Salt ◽

Steviol Glycosides ◽

Rebaudioside A ◽

Tio2 Nps ◽

Stevia Rebaudiana Bertoni

High salt levels are one of the significant and major limiting factors on crop yield and productivity. Out of the available attempts made against high salt levels, engineered nanoparticles (NPs) have been widely employed and considered as effective strategies in this regard. Of these NPs, titanium dioxide nanoparticles (TiO2 NPs) and selenium functionalized using chitosan nanoparticles (Cs–Se NPs) were applied for a quite number of plants, but their potential roles for alleviating the adverse effects of salinity on stevia remains unclear. Stevia (Stevia rebaudiana Bertoni) is one of the reputed medicinal plants due to their diterpenoid steviol glycosides (stevioside and rebaudioside A). For this reason, the current study was designed to investigate the potential of TiO2 NPs (0, 100 and 200 mg L−1) and Cs–Se NPs (0, 10 and 20 mg L−1) to alleviate salt stress (0, 50 and 100 mM NaCl) in stevia. The findings of the study revealed that salinity decreased the growth and photosynthetic traits but resulted in substantial cell damage through increasing H2O2 and MDA content, as well as electrolyte leakage (EL). However, the application of TiO2 NPs (100 mg L−1) and Cs–Se NPs (20 mg L−1) increased the growth, photosynthetic performance and activity of antioxidant enzymes, and decreased the contents of H2O2, MDA and EL under the saline conditions. In addition to the enhanced growth and physiological performance of the plant, the essential oil content was also increased with the treatments of TiO2 (100 mg L−1) and Cs–Se NPs (20 mg L−1). In addition, the tested NPs treatments increased the concentration of stevioside (in the non-saline condition and under salinity stress) and rebaudioside A (under the salinity conditions) in stevia plants. Overall, the current findings suggest that especially 100 mg L−1 TiO2 NPs and 20 mg L−1 Cs–Se could be considered as promising agents in combating high levels of salinity in the case of stevia.

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Lignocellulose@ Activated Clay Nanocomposite with Hierarchical Nanostructure Enhancing the Removal of Aqueous Zn(II)

Polymers ◽

10.3390/polym11101710 ◽

2019 ◽

Vol 11 (10) ◽

pp. 1710 ◽

Cited By ~ 1

Author(s):

Xiaotao Zhang ◽

Yinan Hao ◽

Zhangjing Chen ◽

Yuhong An ◽

Wanqi Zhang ◽

...

Keyword(s):

Ph Value ◽

Removal Rate ◽

Weight Ratio ◽

Order Kinetic ◽

Adsorption Parameters ◽

Hierarchical Nanostructure ◽

Desorption Temperature ◽

Desorption Time ◽

Adsorption Temperature ◽

Hcl Concentration

A lignocellulose@ activated clay (Ln@AC) nanocomposite with a hierarchical nanostructure was successfully synthesized by the chemical intercalation reaction and applied in the removal of Zn(II) from an aqueous solution. Ln@AC was characterized by N2 adsorption/desorption isotherms and X-Ray Diffraction (XRD), scanning Electron Microscope (SEM), transmission Electron Microscopy (TEM) and Fourier Transform Infrared Spectroscopy (FTIR) analysis, and the results indicate that an intercalated–exfoliated hierarchical nanostructure was formed. The effects of different adsorption parameters on the Zn(II) removal rate (weight ratio of Ln to AC, Ln@AC dosage, initial Zn(II) concentration, pH value, adsorption temperature, and time) were investigated in detail. The equilibrium adsorption capacity reached 315.9 mg/g under optimal conditions (i.e., the weight ratio of Ln to AC of 3:1, Ln@AC dosage of 1 g/L, initial Zn(II) concentration of 600 mg/L, pH value of 6.8, adsorption temperature of 65 °C, and adsorption time of 50 min). The adsorption process was described by the pseudo-second-order kinetic model, Langmuir isotherm model, and the Elovich model. Moreover, Zn(II) could be easily eluted by HCl, and the effects of HCl concentration, desorption temperature, and ultrasonic desorption time on desorbed amount were tested. Desorption studies revealed that with an HCl concentration of 0.25 mol/L, desorption temperature of 70 °C, and ultrasonic desorption time of 20 min, the maximum desorption capacity and efficiency were achieved at 202.5 mg/g and 64.10%, respectively. Regeneration experimental results indicated that the Ln@AC exhibited a certain recyclable regeneration performance. Due to such outstanding features, the novel Ln@AC nanocomposite proved to have great adsorption potential for Zn(II) removal from wastewater, and exhibited an extremely significant amount of adsorbed Zn(II) when compared to conventional adsorbents.

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Impact of Glycine Betaine on Salinity Tolerance of Stevia (Stevia rebaudiana Bertoni) under In Vitro Condition

Cercetari Agronomice in Moldova ◽

10.1515/cerce-2017-0029 ◽

2017 ◽

Vol 50 (3) ◽

pp. 95-105 ◽

Cited By ~ 1

Author(s):

V. Rameeh ◽

M. Gerami ◽

V. Ghasemi Omran ◽

S. Ghavampour

Keyword(s):

Glycine Betaine ◽

Stevia Rebaudiana ◽

Mean Value ◽

Rebaudioside A ◽

Stevia Rebaudiana Bertoni ◽

Nacl Concentration ◽

Salinity Levels ◽

High Concentrations

Abstract Stevia (Stevia rebaudiana Bertoni), with great potential as a natural sweeteners source, has a high content of sweeteners, which are up to 150 times sweeter than sugar, but virtually with no calories. Stevia also suitable to be cultivated in semiarid climates and coastal areas, which are characterized by the low quality of the irrigation water. Soil salinity occupies a prominent place among the soil problems that threaten the sustainability of agriculture over a vast area in the world. Glycine betaine is an osmoprotectant, that plays an important role and accumulates rapidly in many plants during salinity or drought stress. In order to evaluation of glycine betaine amending effects on salinity stress in stevia under in vitro condition, a factorial experiment was conducted in 2015. Four NaCl levels, including 0, 50, 75 and 100 mM, along with 0, 1, 12.5, 25 and 50 mM of glycine betaine concentrations were used in Murashige and Skoog (MS) medium. The results showed that salinity levels had significant reduction effects on plant height, root length, shoot fresh weight, number of leaf, total chlorophyll, rebaudioside A and stevioside of the stevia genotype. Due to increasing of glycine betaine, levels all the traits were increased. Owing to amending effect of glycine betaine, its high concentrations made less hazarding effects of salinity on the researched traits. The highest mean value of rebaudioside A (10.62rt) and stevioside (23.38rt) determined at 50 mM of glycine betaine with 0 mM of NaCl concentration.

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Fractionation of Stevia rebaudiana aqueous extracts via two-step ultrafiltration process: towards rebaudioside a extraction

Food and Bioproducts Processing ◽

10.1016/j.fbp.2020.06.010 ◽

2020 ◽

Vol 123 ◽

pp. 111-122 ◽

Cited By ~ 1

Author(s):

Elsa Díaz-Montes ◽

Paulina Gutiérrez-Macías ◽

Carlos Orozco-Álvarez ◽

Roberto Castro-Muñoz

Keyword(s):

Stevia Rebaudiana ◽

Aqueous Extracts ◽

Rebaudioside A ◽

Ultrafiltration Process

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Effect of different drying methods on the composition of steviol glycosides in Stevia rebaudiana Bertoni leaves

International Agrophysics ◽

10.1515/intag-2016-0036 ◽

2017 ◽

Vol 31 (1) ◽

pp. 139-144

Author(s):

Irma Aranda-González ◽

David Betancur-Ancona ◽

Luis Chel-Guerrero ◽

Yolanda Moguel-Ordóñez

Keyword(s):

Low Cost ◽

Stevia Rebaudiana ◽

Hplc Method ◽

Drying Methods ◽

Steviol Glycosides ◽

Rebaudioside A ◽

Drying Method ◽

Sun Drying ◽

Stevia Rebaudiana Bertoni ◽

Rebaudioside D

Abstract Drying techniques can modify the composition of certain plant compounds. Therefore, the aim of the study was to assess the effect of different drying methods on steviol glycosides in Stevia rebaudiana Bertoni leaves. Four different drying methods were applied to Stevia rebaudiana Bertoni leaves, which were then subjected to aqueous extraction. Radiation or convection drying was performed in stoves at 60°C, whereas shade or sun drying methods were applied at 29.7°C and 70% of relative humidity. Stevioside, rebaudioside A, rebaudioside B, rebaudioside C, rebaudioside D, dulcoside A, and steviolbioside were quantified by a validated HPLC method. Among steviol glycosides, the content (g 100 g−1 dry basis) of stevioside, rebaudioside A, rebaudioside B, and rebaudioside C varied according to the drying method. The total glycoside content was higher in sun-dried samples, with no significant differences compared to shade or convection drying, whereas radiation drying adversely affected the content of rebaudioside A and rebaudioside C (p <0.01) and was therefore a method lowering total glycoside content. The effect of the different drying methods was also reflected in the proportion of the sweetener profile. Convection drying could be suitable for modern food processing industries while shadow or sun drying may be a low-cost alternative for farmers.

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