Electrogenerated chemiluminescence peptide-based bioassay

2014 ◽  
Vol 33 (4) ◽  
Author(s):  
Honglan Qi ◽  
Qian Dang ◽  
Manman Dong ◽  
Hongfang Gao ◽  
Min Li
Keyword(s):  
High Sensitivity ◽  
Clinical Analysis ◽  
Electrogenerated Chemiluminescence ◽  
Electrode Surfaces ◽  
Recognition Element ◽  
Response Range ◽  
Future Challenges ◽  
History Of ◽  
Dynamic Concentration ◽  
Molecular Recognition Element

AbstractElectrogenerated chemiluminescence (ECL) involves the generation of species at electrode surfaces that then undergo electron-transfer reactions to form excited states that emit light. The ECL method is becoming an increasingly promising method in the life sciences, environmental analysis, and clinical analysis, owing to its good selectivity, high sensitivity, wide dynamic concentration response range, and potential and spatial controllability. Here, we give a mini-review on the advances of the ECL peptide-based bioassay using peptide as the molecular recognition element. First, a general history of ECL development is presented. Then, the general strategies of the ECL peptide-based bioassay are reviewed. Particular attention is paid to the related progress in the last 5 years. Finally, we conclude with the future challenges and prospects in the development of the ECL peptide-based bioassay.

Strategies for Survival of the World — Risk Management

2019 ◽  
Vol 10 (4) ◽  
Author(s):  
Jovo Lojanica ◽  
Keyword(s):  
Risk Management ◽  
Native Americans ◽  
National Level ◽  
Country Level ◽  
Management Standards ◽  
Family Level ◽  
Future Challenges ◽  
Iso 31000 ◽  
History Of ◽  
Personal Level

All management standards have requirements for different aspects of improvements on the personal level, family level, company level, in business and life. What is about national level and country level? Is it possible for today’s generations to learn history of nations and of civilizations? If it is — ok, let’s apply it on actual time and people to have less problems and difficulties — especially if is actual in field of risk management. Majority of people are occupied by today’s problems. They don’t consider past and future challenges. People from each country strive for better quality, better and cleaner environment, higher safety etc. historically and today. But could we remember: How did Genghis Khan conquer many regions and how was he defeated? How did Mayas and Aztecs die out? How were Native Americans in North America drastically reduced in numbers? How did the Roman Imperium vanish? How was the Ottoman Imperium established and how it vanished? How many people were killed in the wars in XX century, etc? In all these catastrophic changes risks were not considered in an adequate way. Requirements of risk management — Principles and guidelines — ISO 31000:2009 are very consultative. They could be used on country level, national level, regional level, continental and intercontinental level.

Pollen-Shaped Hierarchical Structure for Pressure Sensors with High Sensitivity in an Ultrabroad Linear Response Range

2020 ◽  
Vol 12 (49) ◽  
pp. 55362-55371
Author(s):  
Tingting Zhao ◽  
Li Yuan ◽  
Tongkuai Li ◽  
Longlong Chen ◽  
Xifeng Li ◽  
...  
Keyword(s):  
Hierarchical Structure ◽  
Linear Response ◽  
Pressure Sensors ◽  
High Sensitivity ◽  
Response Range ◽  
Linear Response Range

scholarly journals Enhanced specificity of clinical high-sensitivity tumor mutation profiling in cell-free DNA via paired normal sequencing using MSK-ACCESS

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
A. Rose Brannon ◽  
Gowtham Jayakumaran ◽  
Monica Diosdado ◽  
Juber Patel ◽  
Anna Razumova ◽  
...  
Keyword(s):  
Cancer Patients ◽  
De Novo ◽  
Low Frequency ◽  
High Sensitivity ◽  
Clinical Analysis ◽  
De Novo Mutation ◽  
Cell Free Dna ◽  
Free Dna ◽  
Somatic Alterations ◽  
Mutation Profiling

AbstractCirculating cell-free DNA from blood plasma of cancer patients can be used to non-invasively interrogate somatic tumor alterations. Here we develop MSK-ACCESS (Memorial Sloan Kettering - Analysis of Circulating cfDNA to Examine Somatic Status), an NGS assay for detection of very low frequency somatic alterations in 129 genes. Analytical validation demonstrated 92% sensitivity in de-novo mutation calling down to 0.5% allele frequency and 99% for a priori mutation profiling. To evaluate the performance of MSK-ACCESS, we report results from 681 prospective blood samples that underwent clinical analysis to guide patient management. Somatic alterations are detected in 73% of the samples, 56% of which have clinically actionable alterations. The utilization of matched normal sequencing allows retention of somatic alterations while removing over 10,000 germline and clonal hematopoiesis variants. Our experience illustrates the importance of analyzing matched normal samples when interpreting cfDNA results and highlights the importance of cfDNA as a genomic profiling source for cancer patients.

scholarly journals The Swiss Industrial Biocatalysis Consortium (SIBC): Past, Present and Future

2020 ◽  
Vol 74 (5) ◽  
pp. 342-344
Author(s):  
Steven Hanlon
Keyword(s):  
Knowledge Sharing ◽  
Fine Chemicals ◽  
Special Edition ◽  
Founding Fathers ◽  
Future Challenges ◽  
History Of

Since its inception in 2004, the Swiss Industrial Biocatalysis Consortium (SIBC) has brought together scientists from the Pharma, Fine Chemicals, Agrochemicals and Flavor and Fragrance Industries with the goal of promoting biocatalysis inside and outside of Switzerland as well as providing mutual benefits in the form of pre-competitive knowledge sharing. One of the 'founding fathers' of the SIBC was of course Oreste Ghisalba, whom we are honoring here in this special edition. The history of the SIBC as well as current activities and future challenges will be presented.

On-Line Documentation Systems: History and Issues

1983 ◽  
Vol 27 (13) ◽  
pp. 1027-1030
Author(s):  
Joseph J. Limanowski
Keyword(s):  
Human Factors ◽  
Data Processing ◽  
Electronic Data Processing ◽  
Documentation System ◽  
Electronic Data ◽  
Future Challenges ◽  
History Of ◽  
On Line ◽  
Documentation Systems ◽  
Paper Documentation

The present paper provides a brief history of Electronic Data Processing Systems and their supporting user documentation. The problems inherent to paper documentation are highlighted. Techniques employed to improve on-line reject messages in addition to the development of an online user documentation system are discussed. Future challenges facing the human factors specialist involved in development of on-line documentation are outlined.

scholarly journals Patients With a History of Idiopathic Deep Venous Thrombosis Have Long-Term Increased Levels of Inflammatory Markers and Markers of Endothelial Damage

2016 ◽  
Vol 23 (2) ◽  
pp. 124-131 ◽  
Author(s):  
Mateja Kaja Jezovnik ◽  
Jawed Fareed ◽  
Pavel Poredos
Keyword(s):  
Inflammatory Markers ◽  
High Sensitivity ◽  
Endothelial Damage ◽  
Control Group ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Inflammatory Parameters ◽  
History Of ◽  
Hemostatic Markers

Introduction: Although the role of inflammation in DVT has been investigated in different studies, there is no definite answer as to whether increased systemic inflammation is the cause or the consequence of DVT. Aim: To follow inflammatory parameters in a cohort of patients with idiopathic DVT. Methods: Out of 49 patients with an acute idiopathic DVT, which were investigated four months after an acute episode (DEVTA 1), 43 patients were included in the follow-up study investigating inflammatory markers and hemostatic markers of endothelial damage five years after an acute DVT (DEVTA 2). A control group consisted of 43 sex and age matched healthy subjects (CONTROLS). Results: The levels of inflammatory markers were significantly higher in DEVTA 2 in comparison to CONTROLS: tumor necrosis factor alpha 2.0 pg/mL (1.1-2.3) vs 1.3 pg/mL (0.8-1.9), p < .001, high sensitivity C-reactive protein 3.2 mg/L (1.5-5.2) vs 1.7 mg/L (0.9-3.0), p = .008, interleukin-6 (IL-6) 2.7 pg/mL (2.0-3.5) vs 2.1 pg/mL (1.5-2.6), p = .025, IL-8 5.0 pg/mL (3.6-7.3) vs 2.4 pg/mL (1.8-2.8), p < .001. IL-10 was significantly decreased (0.9 pg/mL (0.7-1.8) vs 1.8 (1.5-2.2), p < .001. Most of the proinflammatory markers remained elevated in the DEVTA 2 in comparison to DEVTA 1. Markers of endothelial damage were higher in DEVTA 2 in comparison to CONTROLS and higher than in DEVTA 1. Conclusion: Patients with idiopathic DVT have long-term increased inflammatory markers and markers of endothelial damage. These findings favor the hypothesis that inflammation is a cause and not merely a consequence of acute DVT.

Detection of Viruses: insides of antibody-antigen interactions and Multifunctional Lab-On-particle for SARS CoV-2 detection

Coronaviruses ◽  
2021 ◽  
Vol 02 ◽  
Author(s):  
Martin Amé ◽  
Esraa Samy Abu Serea ◽  
Ahmed Esmail Shalan ◽  
A. Guillermo Bracamonte
Keyword(s):  
Fast Response ◽  
Clinical Analysis ◽  
New Drugs ◽  
Detection Systems ◽  
Current State ◽  
History Of ◽  
Non Covalent Interactions ◽  
Genomic Factor ◽  
New Treatments ◽  
Covalent Interactions

: The design of new Drugs, Molecules, Nano-, and Microstructures for targeted applications along the pass of the time within the history of Chemistry and Clinical Analysis showed to be of high impact on Life Sciences. In this context, and mainly for the actual Corona Virus Pandemic status, it should be highlighted the importance of a fast response. Thus, the developments of differents approaches looking for improved and higher sensitive solutions in the Biodetection field accompanied with new treatments of SARS CoV-2 are required. In this way, in this short communication based on the latest Research publications it was afforded and discussed the following topics: i) the importance of non-covalent interactions from antibody-antigen recognition events, ii) the genomic factor, and iii) the use of non-covalent interactions in different Optical detection systems and Lab-On particles. Moreover, it was afforded the theme related with the importance of new treatments in progress, such development of new vaccines; and current state of the pandemic situation. So, it was discussed and stimulated the improvement of innovative Multifunctional Nanophotonic approaches intended for SARS CoV-2 detection accompanied with potential targeted treatments.

Phage as a molecular recognition element in biosensors immobilized by physical adsorption

2007 ◽  
Vol 22 (6) ◽  
pp. 986-992 ◽  
Author(s):  
Viswaprakash Nanduri ◽  
Iryna B. Sorokulova ◽  
Alexandre M. Samoylov ◽  
Aleksandr L. Simonian ◽  
Valery A. Petrenko ◽  
...  
Keyword(s):  
Molecular Recognition ◽  
Physical Adsorption ◽  
Recognition Element ◽  
Molecular Recognition Element
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