In vitro degradation and bioactivity of poly(propylene fumarate)/bioactive glass sintered microsphere scaffolds for bone tissue engineering

2016 ◽  
Vol 23 (3) ◽  
pp. 245-256 ◽  
Author(s):  
Sima Shahabi ◽  
Yashar Rezaei ◽  
Fathollah Moztarzadeh ◽  
Farhood Najafi
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
P Uptake ◽  
Surface Reactivity ◽  
Ion Concentration ◽  
Composite Scaffolds ◽  
Poly Propylene

AbstractWe developed degradable poly(propylene fumarate)/bioactive glass (PPF/BG) composite scaffolds based on a sintered microsphere technique and investigated the effects of BG content on the characteristics of these composite scaffolds. Immersion in a simulated body fluid (SBF) was used to evaluate the surface reactivity of composite scaffolds. The surface of composite scaffolds was covered with hydroxycarbonate apatite layer after 7 days of immersion. Ion concentration analyses revealed a decrease in P concentration and an increase in Si, Ca, and Sr concentrations in SBF immersed with composite scaffolds during the 3-week period. The Ca and P uptake rates decreased after 4 days of incubation. This coincided with the decrease of the Si release rate. These data lend support to the suggestion that the Si released from the BG content of scaffolds present in the polymer matrix was involved in the formation of the Ca-P layer. The evaluation of the in vitro degradation of composite microspheres revealed that the weight of scaffolds remained relatively constant during the first 3 weeks and then started to decrease slowly, losing 10.5% of their initial mass by week 12. Our results support the concept that these new bioactive, degradable composite scaffolds may be used for bone tissue engineering applications.

scholarly journals Excellency of Hydroxyapatite Composite Scaffolds for Bone Tissue Engineering

Biomaterials ◽  
2020 ◽  
Author(s):  
Mohammad Shariful Islam ◽  
Mohammad Abdulla-Al-Mamun ◽  
Alam Khan ◽  
Mitsugu Todo
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Surface Reactivity ◽  
Growth Factor Delivery ◽  
Composite Scaffolds ◽  
In Vivo Experiments ◽  
Hydroxyapatite Composite

The hydroxyapatite [HAp, Ca10(PO4)6(OH)2] has a variety of applications in bone fillers and replacements due to its excellent bioactivity and osteoconductivity. It comprises the main inorganic component of hard tissues. Among the various approaches, a composite approach using several components like biopolymer, gelatin, collagen, and chitosan in the functionalization of scaffolds with HAp has the prospective to be an engineered biomaterial for bone tissue engineering. HAp composite scaffolds have been developed to obtain a material with different functionalities such as surface reactivity, bioactivity, mechanical strength, and capability of drug or growth factor delivery. Several techniques and processes for the synthesis and fabrication of biocompatible HAp composite scaffolds suitable for bone regeneration are addressed here. Further, this chapter described the excellences of various HAp composite scaffolds used in in vitro and in vivo experiments in bone tissue engineering.

scholarly journals Hydroxyapatite Whisker Reinforced 63s Glass Scaffolds for Bone Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Cijun Shuai ◽  
Yiyuan Cao ◽  
Chengde Gao ◽  
Pei Feng ◽  
Tao Xiao ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Fracture Toughness ◽  
Tissue Engineering ◽  
Compressive Strength ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Selective Laser Sintering ◽  
Composite Scaffolds

Bioactive glass (BG) is widely used for bone tissue engineering. However, poor mechanical properties are the major shortcomings. In the study, hydroxyapatite nanowhisker (HANw) was used as a reinforcement to improve the mechanical properties. 63s glass/HANw scaffolds were successfully fabricated by selective laser sintering (SLS). It was found that the optimal compressive strength and fracture toughness were achieved when 10 wt.% HANw was added. This led to 36% increase in compressive strength and 83% increase in fracture toughness, respectively, compared with pure 63s glass scaffolds. Different reinforcement mechanisms were analyzed based on the microstructure investigation. Whisker bridging and whisker pulling-out were efficient in absorbing crack propagating energy, resulting in the improvement of the mechanical properties. Moreover, bioactivity and biocompatibility of the scaffolds were evaluated in vitro. The results showed that composite scaffolds with 10 wt.% HANw exhibited good apatite-forming ability and cellular affinity.

scholarly journals Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

2020 ◽  
Vol 6 (1) ◽  
pp. 57-69
Author(s):  
Amirhosein Fathi ◽  
Farzad Kermani ◽  
Aliasghar Behnamghader ◽  
Sara Banijamali ◽  
Masoud Mozafari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

Innovative Composite HA Scaffold Rapid Prototyping for Bone Reconstruction: An In Vitro Pilot Study

2013 ◽  
Vol 583 ◽  
pp. 56-63 ◽  
Author(s):  
Isidoro Giorgio Lesci ◽  
Leonardo Ciocca ◽  
Barbara Dozza ◽  
Enrico Lucarelli ◽  
Sergio Squarzoni ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
Pilot Study ◽  
Rapid Prototyping ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
High Surface ◽  
Surface Reactivity ◽  
Macro Scale

The ability to control the architecture and strength of a bone tissue engineering scaffold is critical to achieve a harmony between the scaffold and the host tissue. The scaffold attempts to mimic the function of the natural extracellular matrix, providing a temporary template for the growth of target tissues. The study of nanocrystalline calcium phosphate physical-chemical characteristics and, thereafter, the possibility to imitate bone mineral for the development of new advanced biomaterials is constantly growing. Scaffolds should have suitable architecture and strength to serve their intended function. Rapid prototyping (RP) technique is applied to tissue engineering to satisfy this need and to create a scaffold directly from the scanned and digitized image of the defect site. Design and construction of complex structures with different shapes and sizes, at micro and macro scale, with fully interconnected pore structure and appropriate mechanical properties are possible by using RP techniques. In this study we present a new biocompatible hybrid scaffold obtained through two different experimental methods and formed by synthetic biomimetic Hydroxyapatite (HA) nanocrystals with high surface reactivity which synergistically interacts with Poly(e-caprolactone) (PCL) and polylactic acid (PLLA). The aim of this pilot study is to test the adhesion and the proliferation of human mesenchymal stem cells (MSC) on both the scaffolds. MSC growth and distribution was evaluated 24 h and 7 days after in-vitro seeding. The results allowed the conclusion that these scaffolds are biocompatible and allow the colonization and proliferation of MSC, therefore, due to their mechanical properties, they are adequate for bone tissue engineering.

scholarly journals In vitro and in vivo Biocompatibility of Alginate Dialdehyde/Gelatin Hydrogels with and without Nanoscaled Bioactive Glass for Bone Tissue Engineering Applications

Materials ◽  
2014 ◽  
Vol 7 (3) ◽  
pp. 1957-1974 ◽  
Author(s):  
Ulrike Rottensteiner ◽  
Bapi Sarker ◽  
Dominik Heusinger ◽  
Diana Dafinova ◽  
Subha Rath ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Engineering Applications
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