Oxidative Stress Parameters after Abdominal Hysterectomy and Their Relationships with Quality of Recovery

Abstract Study aimed to investigate relationship between oxidative stress markers and postoperative recovery in woman after abdominal hysterectomy, as well as to test the hypothesis that different analgesics differently influence redox status. The quality of recovery was evaluated with a QoR-40 questionnaire in fifty-one patients who underwent abdominal hysterectomy, preoperatively and on the 1st, 2nd, 3rd postoperative days (POD1,2,3). Blood samples were collected at baseline (T0), 3 (T1), 24 (T2), 48 (T3) and 72 (T4) hours after surgery. Oxidative stress markers concentrations (TBARS, NO2−, H2O2, O2− ) as well as antioxidative enzymes (SOD, CAT, and GSH) were analyzed. QoR-40 total score significantly declined on POD1 and POD2 and returned to baseline levels on POD3 (p<0.001). H2O2 levels significantly decreased from T0 to T3 and then, increased at T4 (p=0,011). Changes of TBARS and H2O2 from T0 to T3 showed significant and negative correlation (r=−0.303, p=0.046). There was no significant correlation between QoR-40 total score and any parameter of oxidative stress response (p>0.05). Changes in TBARS levels from T0 to T3 were statistically significant between the study subgroups primarily due to increase of the concentrations in patients receiving paracetamol (p=0.031). Patients age, duration of surgery and cigarette smoking status showed significant influcences on and association with some oxidative stress response markers (TBARS, O2−, CAT) (p<0.05). Women who underwent hysterectomy had significant changes of H2O2 and TBARS activity however, those changes were not associated with changes of QoR-40 total scores during recovery.

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Physiopathological Bases of the Disease Caused by HACE1 Mutations: Alterations in Autophagy, Mitophagy and Oxidative Stress Response

Journal of Clinical Medicine ◽

10.3390/jcm9040913 ◽

2020 ◽

Vol 9 (4) ◽

pp. 913

Author(s):

Olatz Ugarteburu ◽

Marta Sánchez-Vilés ◽

Julio Ramos ◽

Tamara Barcos-Rodríguez ◽

Gloria Garrabou ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Oxidative Damage ◽

Neurodevelopmental Disorder ◽

Psychomotor Retardation ◽

Oxidative Stress Response ◽

Autophagic Flux ◽

Mrna Levels ◽

Mitochondrial Oxidative Stress ◽

Stress Markers

Recessive HACE1 mutations are associated with a severe neurodevelopmental disorder (OMIM: 616756). However, the physiopathologycal bases of the disease are yet to be completely clarified. Whole-exome sequencing identified homozygous HACE1 mutations (c.240C>A, p.Cys80Ter) in a patient with brain atrophy, psychomotor retardation and 3-methylglutaconic aciduria, a biomarker of mitochondrial dysfunction. To elucidate the pathomechanisms underlying HACE1 deficiency, a comprehensive molecular analysis was performed in patient fibroblasts. Western Blot demonstrated the deleterious effect of the mutation, as the complete absence of HACE1 protein was observed. Immunofluorescence studies showed an increased number of LC3 puncta together with the normal initiation of the autophagic cascade, indicating a reduction in the autophagic flux. Oxidative stress response was also impaired in HACE1 fibroblasts, as shown by the reduced NQO1 and Hmox1 mRNA levels observed in H2O2-treated cells. High levels of lipid peroxidation, consistent with accumulated oxidative damage, were also detected. Although the patient phenotype could resemble a mitochondrial defect, the analysis of the mitochondrial function showed no major abnormalities. However, an important increase in mitochondrial oxidative stress markers and a strong reduction in the mitophagic flux were observed, suggesting that the recycling of damaged mitochondria might be targeted in HACE1 cells. In summary, we demonstrate for the first time that the impairment of autophagy, mitophagy and oxidative damage response might be involved in the pathogenesis of HACE1 deficiency.

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Increased Oxidative Stress Response in Granulocytes from Older Patients with a Hip Fracture May Account for Slow Regeneration

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/819847 ◽

2014 ◽

Vol 2014 ◽

pp. 1-9 ◽

Cited By ~ 3

Author(s):

Zhiyong Wang ◽

Sabrina Ehnert ◽

Christoph Ihle ◽

Lilianna Schyschka ◽

Stefan Pscherer ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Age Groups ◽

Young Patients ◽

The Elderly ◽

Oxidative Stress Response ◽

Proximal Femur Fracture ◽

Mapk Activation ◽

Stress Markers ◽

Stress Response System

Proximal femur fracture, a typical fracture of the elderly, is often associated with morbidity, reduced quality of life, impaired physical function and increased mortality. There exists evidence that responses of the hematopoietic microenvironment to fractures change with age. Therefore, we investigated oxidative stress markers and oxidative stress-related MAPK activation in granulocytes from the young and the elderly with and without fractured long bones. Lipid peroxidation levels were increased in the elderly controls and patients. Aged granulocytes were more sensitive towards oxidative stress induced damage than young granulocytes. This might be due to the basally increased expression of SOD-1 in the elderly, which was not further induced by fractures, as observed in young patients. This might be caused by an altered MAPK activation. In aged granulocytes basal p38 and JNK activities were increased and basal ERK1/2 activity was decreased. Following fracture, JNK activity decreased, while ERK1/2 and p38 activities increased in both age groups. Control experiments with HL60 cells revealed that the observed p38 activation depends strongly on age. Summarizing, we observed age-dependent changes in the oxidative stress response system of granulocytes after fractures, for example, altered MAPK activation and SOD-1 expression. This makes aged granulocytes vulnerable to the stress stimuli of the fracture and following surgery.

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MON-161 ASSESSMENT OF CIRCULATING OXIDATIVE STRESS MARKERS, INFLAMMATORY MARKERS AND THEIR CORRELATION WITH QUALITY OF LIFE IN PATIENTS ON MAINTENANCE HEMODIALYSIS

Kidney International Reports ◽

10.1016/j.ekir.2019.05.954 ◽

2019 ◽

Vol 4 (7) ◽

pp. S368

Author(s):

D.K.J. PRIYADARSHINI

Keyword(s):

Quality Of Life ◽

Oxidative Stress ◽

Inflammatory Markers ◽

Maintenance Hemodialysis ◽

Oxidative Stress Markers ◽

Stress Markers

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Cottonseed feeding changes oxidative stress markers in ewes during the peripartum period and increases the quality of colostrum

The Veterinary Journal ◽

10.1016/j.tvjl.2019.02.009 ◽

2019 ◽

Vol 247 ◽

pp. 32-37 ◽

Cited By ~ 1

Author(s):

Debora B. Moretti ◽

Carolina R. Jimenez ◽

Heloisa M. Trinca ◽

Raul Machado-Neto ◽

Helder Louvandini

Keyword(s):

Oxidative Stress ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Peripartum Period

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Oxidative Stress Markers Are Associated with Persistent Atrial Fibrillation

Clinical Chemistry ◽

10.1373/clinchem.2006.083923 ◽

2007 ◽

Vol 53 (9) ◽

pp. 1652-1657 ◽

Cited By ~ 139

Author(s):

Robert B Neuman ◽

Heather L Bloom ◽

Irfan Shukrullah ◽

Lyndsey A Darrow ◽

David Kleinbaum ◽

...

Keyword(s):

Oxidative Stress ◽

Atrial Fibrillation ◽

Inflammatory Markers ◽

Smoking Status ◽

Serum Markers ◽

Oxidative Stress Markers ◽

Cross Sectional ◽

Stress Markers ◽

Myocardial Oxidative Stress ◽

Antioxidant Agents

Abstract Background: Atrial fibrillation (AF) has been associated with myocardial oxidative stress, and antioxidant agents have demonstrated antiarrhythmic benefit in humans. We compared serum markers of oxidation and associated inflammation in individuals with or without AF. Methods: Serum markers of oxidative stress and inflammation were compared in a cross-sectional, case-control design study of 40 male individuals, with or without persistent or permanent AF, who were matched for age, sex, diabetes, and smoking status, known confounding variables for the measurement of oxidative stress. We used derivatives of reactive oxidative metabolites (DROMs) and ratios of oxidized to reduced glutathione (Eh GSH) and cysteine (Eh CySH) to quantify oxidative stress. We also measured inflammatory markers, including high-sensitivity C-reactive protein, interleukins 1β and 6, and tumor necrosis factor α. Results: Univariate, conditional logistical regression analysis showed that oxidative stress but not inflammatory markers were statistically associated with AF (P <0.05). The increase in the odds ratios for AF for Eh GSH, Eh CySH, and DROMs were 6.1 (95% CI, 1.3–28.3; P = 0.02), 13.6 (95% CI, 2.5–74.1; P = 0.01), and 15.9 (95% CI, 1.7–153.9; P = 0.02), respectively. There was a stronger correlation between Eh GSH and Eh CySH (r = 0.66) than between Eh GSH and DROMs (r = 0.41). In multivariate analysis corrected for statins and other AF risk factors differing between the groups, the association of AF and oxidative stress remained significant. Conclusions: These data suggest that oxidative stress markers may have predictive value in AF management.

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Black and yellow soybean: Contribution of seed quality to oxidative stress response during plant development

Genetika ◽

10.2298/gensr1902495k ◽

2019 ◽

Vol 51 (2) ◽

pp. 495-510

Author(s):

Biljana Kiprovski ◽

Jegor Miladinovic ◽

Anamarija Koren ◽

Djordje Malencic ◽

Maja Mikulic-Petkovsek

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Phenolic Compounds ◽

Seed Quality ◽

Oxidative Stress Response ◽

Oxidative Stresses ◽

Conventional Production ◽

Reproduction Period ◽

Excellent Source

The purpose of this paper was to compare quality of seeds of two black and yellow soybean and the response of plants developed from these seeds to oxidative stress during vegetation and reproduction period. Content of carbohydrates: cellulose, starch, total and reduced sugars, as well as oil and protein content varied among all varieties, irrespective the colour. Bearing in mind all differences in seed quality, as well as response to oxidative stresses during development, black cultivars could be proposed as an excellent source of phenolic compounds (flavan-3-ols, antocyanins, genistein, glycitein, quercetin, laricitrin and isorhamnetin derivatives). Due to extreme fluctuation in precipitation amounts in the last years, information on the better performance of soybean varieties in oxidative stress conditions is of great importance to organic and conventional production of this cultivar.

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Inducing the oxidative stress response in Escherichia coli improves the quality of a recombinant protein: Magnesium chelatase ChlH

Protein Expression and Purification ◽

10.1016/j.pep.2014.06.004 ◽

2014 ◽

Vol 101 ◽

pp. 61-67 ◽

Cited By ~ 6

Author(s):

André H. Müller ◽

Artur Sawicki ◽

Shuaixiang Zhou ◽

Shabnam Tarahi Tabrizi ◽

Meizhong Luo ◽

...

Keyword(s):

Oxidative Stress ◽

Escherichia Coli ◽

Stress Response ◽

Recombinant Protein ◽

Oxidative Stress Response ◽

Magnesium Chelatase

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Muscle Cortisol Levels, Expression of Glucocorticoid Receptor and Oxidative Stress Markers in the Teleost Fish Argyrosomus regius Exposed to Transport Stress

Animals ◽

10.3390/ani11041160 ◽

2021 ◽

Vol 11 (4) ◽

pp. 1160

Author(s):

Martina Bortoletti ◽

Lisa Maccatrozzo ◽

Giuseppe Radaelli ◽

Stefano Caberlotto ◽

Daniela Bertotto

Keyword(s):

Oxidative Stress ◽

Stress Response ◽

Glucocorticoid Receptor ◽

Control Level ◽

Fish Muscle ◽

Oxidative Stress Markers ◽

Stress Markers ◽

Argyrosomus Regius ◽

Transport Stress ◽

Cortisol Levels

Fish commercial transport is an ordinary practice in the aquaculture industry. This study aimed to investigate the effect of a 48 h transport stress on stress response of meagre (Argyrosomus regius) juveniles. Radioimmunoassay (RIA) and Real-Time PCR were used to evaluate muscle cortisol levels and to assess glucocorticoid receptor (gr) gene expression in fish muscle and liver, respectively. Presence and localization of various oxidative stress markers were investigated in different tissues by immunohistochemistry. A significant increase in muscle cortisol levels was observed after loading but a significant decrease occurred after 16 h from departure even without returning to control levels. Molecular analysis on stress response revealed an increase in muscle gr expression after fish loading that started decreasing during the travel returning to the control level at the end of the transport. Instead, no differences in liver gr expression were observed along the different sampling points. Immunostaining for heat shock protein 70 (HSP70), 4-hydroxy-2-nonenal (HNE), nitrotyrosine (NT) and 8-hydroxy-2’-deoxyguanosine (8-OHdG) antibodies was detected in several organs. Notably, a higher NT immunostaining intensity was evident in skin and gills of the transported animals with respect to controls. Results demonstrated that cortisol and gr are useful indicators of stressful conditions in transported fish.

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