Indol-3-essigsäure induzierte Calciumionen- abgabe bei Maiskoleoptilzylindern / 3-Indol Acetic Acid Induced Efflux of Calciumions by Maize Coleoptile Sections

1973 ◽  
Vol 28 (11-12) ◽  
pp. 777-778 ◽  
Author(s):  
Boris Janistyn
Keyword(s):  
Acetic Acid ◽  
Maize Coleoptile ◽  
Indol Acetic Acid

Abstract 3-Indol acetic acid, calcium, adenosin-3':5'-monophosphat (c-AMP), maize coleoptile sections

Growth and Aflatoxin Production by Aspergillus parasiticus in a Medium Containing Plant Hormones, Herbicides or Insecticides

1987 ◽  
Vol 50 (12) ◽  
pp. 1044-1047 ◽  
Author(s):  
R. S. FARAG ◽  
M. A. EL-LEITHY ◽  
A. E. BASYONY ◽  
Z. Y. DAW
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Plant Hormones ◽  
Aspergillus Parasiticus ◽  
Synthetic Medium ◽  
Fungal Growth ◽  
Aflatoxin Production ◽  
Inhibitory Effect ◽  
Indol Acetic Acid ◽  
Acid Herbicides

The effect of some widely used plant hormones (indol-3-acetic acid and gibberellic acid), herbicides (gramoxone, stomp and treflan) and insecticides (malathion, actellic and guthion) on Aspergillus parasiticus growth and aflatoxin production in a synthetic medium was studied. Addition of indol acetic acid to the medium increased aflatoxin production more than gibberellic acid. Treflan at 5, 10 and 20 ppm levels caused a highly significant stimulatory effect on A. parasiticus growth and aflatoxin production. In contrast, stomp at 10 and 20 ppm produced the reverse effect. Guthion, an insecticide, caused a marked decrease in fungal growth and aflatoxin production. The inhibitory effect of insecticides under study on both fungal growth and aflatoxin production in effectiveness followed the sequence: guthion>actellic>malathion. At the recommended application rate (10 ppm), with the exception of indol acetic acid and treflan, all compounds suppressed mold growth and aflatoxin production.

Production de composés indoliques rhizogènes par le ver de terre Lumbricus terrestris

2001 ◽  
Vol 79 (11) ◽  
pp. 1921-1932 ◽  
Author(s):  
Abdellatif El Harti ◽  
Mohamed Saghi ◽  
J -AE Molina ◽  
Gérard Teller
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Lumbricus Terrestris ◽  
Polar Group ◽  
Bean Plants ◽  
Indol Acetic Acid ◽  
Layer Chromatography ◽  
Group Of Fractions

In vitro application of total gross extract of earthworms (Lumbricus terrestris) in diverse dilutions stimulates rhizogenesis in young bean plants (Phaseolus vulgaris). The observed effect is similar to that of indol acetic acid, a well-known growth enhancer in plants, used here as a control in various concentrations. Fragmentation of worm extract by column chromatography results in three groups of fractions. Only the polar group of fractions has a significant rhizogenous effect, which is, however, inferior to that observed in the presence of total gross extract of worms or of indol acetic acid. Gross extract analyses using thin layer chromatography, with appropriate chromatography systems and reagents, revealed that indol acetic acid is not present, but is probably replaced by other indol-derived substances that have a neutral to basic chromatographic behaviour. These presumed indol-derived substances are identified as methyl-tryptophane, serotonin, and hydroxy-indol acetic acid. Analyses using mass spectrometry combined with gas chromatography, following fragmentation and purification of the group of rhyzogenous fractions, have revealed the presence of hydroxy-indol carboxylic acid, which seems to take the form of several isomeres.[Journal translation]

scholarly journals Optimisasi dan pemurnian IAA yang dihasilkan Rhizobium sp. dalam medium serum lateks dengan suplementasi triptofan dari pupuk kandang Optimization and purification of IAA produced by Rhizobium sp. in latex serum media supplemented with tryptophan from chicken manure

2016 ◽  
Vol 76 (2) ◽  
Author(s):  
Irma KRESNAWATY ◽  
Syeda ANDANAWARIH ◽  
. SUHARYANTO ◽  
. TRI-PANJI
Keyword(s):  
Acetic Acid ◽  
Chicken Manure ◽  
Growth Media ◽  
High Concentration ◽  
Carbon And Nitrogen ◽  
Indol Acetic Acid ◽  
Layer Chromatography ◽  
Hydrolysis Of ◽  
Rhizobium Sp ◽  
Direct Hydrolysis

Summary Concentrated latex effluent had not been economically utilized, consequently it had become source of environmental pollution and conflicts with surrounding community. Whereas, the concentrated latex effluent could be used as substrate for microbes growth media due to its high concentration of carbon and nitrogen. One of the economical benefits of growing Rhizobium sp. in this waste is the production of  indole acetic acid (IAA) that  can be used for plant promotion growth. The aims of this research were to get the optimal IAA production of Rhizobium sp. by optimizing its tryptophan supplementation through hydrolysis of chicken manure and to purify IAA produced using chromatographic method. The use of chicken manure directly caused the browning effect, therefore these experiments were carried out the variation of NaOH 2 N hydrolysis treatments to reduce the effect. Direct hydrolysis as the first media  was obtained by mixing latex serum and manure, and then this mixture was hydrolyzed. Meanwhile, separated hydrolysis was done by adding water to manure, being hydrolyzed, and divided to become second and third media. The second media  was made by mixing manure hydrolysate and latex serum directly, whereas in third media, hydrolisate was added with alum as coagulating agent. Rhizobium sp. was then inoculated to all media and incubated for 24, 48, and 72 hours in 27-30oC. IAA was analyzed by spectrophotometric method with Salkowsky reagent and Thin Layer Chromatography (TLC). IAA was then extracted with ethyl acetate and purified with silica gel column chromatography. The separated hydrolysis without coagulation (second media) produced the highest IAA concentration, that is 14.40 mg/mL, whereas IAA produced by direct hydrolysis (first media) was 14.13 mg/mL and 0.90 mg/mL for third media  during 48 hours. The fractionation result  for each mediums showed that the highest IAA distribution in first media  was the 12th fraction (38.70%), meanwhile in second media  was the 15th fraction (50.25%) and in the third  media was the 13th fraction (26.16%). Ringkasan Limbah lateks pekat saat ini belum di-manfaatkan secara ekonomis, bahkan menjadi sumber pencemaran lingkungan dan konflik dengan masyarakat sekitarnya. Padahal limbah lateks pekat dapat digunakan sebagai substrat pertumbuhan mikroba karena memiliki kandungan karbon dan nitrogen yang cukup tinggi.  Salah  satu  nilai  ekonomis yang dapat diperoleh dengan ditumbuhkannya Rhizobium sp. pada limbah tersebut, yaitu dihasilkannya asam indol asetat (indol acetic acid/IAA) yang dapat digunakan untuk memacu pertumbuhan tanaman. Penelitian ini bertujuan memperoleh produksi IAA optimal yang dihasilkan Rhizobium sp. dengan asupan triptofan dari hidrolisis pupuk kandang dan memurnikan IAA yang dihasilkan tersebut dengan metode kromatografi. Penggunaan pupuk kandang secara langsung menyebabkan efek pen-cokelatan, maka dilakukan variasi perlakuan hidrolisis dengan NaOH 2 N untuk mengurangi efek tersebut. Hidrolisis langsung sebagai medium pertama diperoleh dengan mencampur serum lateks dan pupuk kandang, sedangkan hidrolisis terpisah dilakukan dengan menambah pupuk kandang dengan air,  dan dibagi menjadi medium kedua dan ketiga. Medium kedua dibuat dengan cara  langsung mencampur hidrolisat dan serum lateks, sedangkan pada medium ketiga, hidrolisat diendapkan dengan alum sebagai bahan pengendap.  Kemudian ke dalam masing-masing medium diinokulasi  Rhizobium sp. dan diinkubasi selama 24 ,48, dan 72 jam pada suhu 27-30oC. Analisis IAA dilakukan secara spektrofotometri dengan metode Salkowski dan Kromatografi Lapis Tipis (KLT). IAA diekstraksi menggunakan etil asetat dan dimurnikan dengan kromatografi kolom silika gel. Hidrolisis terpisah tanpa pengendapan (medium kedua) menghasilkan IAA tertinggi, yaitu 14,40 mg/mL, sedangkan hidrolis langsung (medium pertama) menghasilkan IAA sebesar 14,13 mg/mL dan medium ketiga sebesar 0,90 mg/mL selama 48 jam. Hasil fraksinasi untuk masing-masing medium menunjukkan sebaran IAA tertinggi pada medium pertama berada pada fraksi ke-12 (38,70%), sedangkan pada medium kedua pada fraksi ke-15 (50,25%), dan pada medium ketiga ialah fraksi ke-13 (26,16%). 

scholarly journals KARAKTERISASI DAN IDENTIFIKASI BAKTERI RIZOSFER TANAMAN SAWIT JAMBI

2019 ◽  
Vol 12 (2) ◽  
pp. 198-206
Author(s):  
Khairani Khairani ◽  
Fitratul Aini ◽  
Hesti Riany
Keyword(s):  
Acetic Acid ◽  
Oil Palm ◽  
Pathogenic Bacteria ◽  
Biochemical Characterization ◽  
Growth Hormones ◽  
Rhizosphere Bacteria ◽  
Acid Phosphate ◽  
Bacterial Genus ◽  
Indol Acetic Acid ◽  
Conventional Methods

AbstrakBakteri rizosfer adalah bakteri yang terdapat pada daerah perakaran tanaman yang diketahui memiliki keanekaragaman tinggi. Bakteri rizosfer memiliki berbagai peran seperti menyediakan nutrisi bagi tanaman, melindungi tanaman dari infeksi bakteri patogen, menghasilkan hormon pertumbuhan seperti indol acetic acid, pelarut fosfat, pengikat nitrogen, dan lain-lain. Dengan berbagai kemampuan dan peran tersebut, maka perlu eksplorasi bakteri rizosfer tanaman sawit khususnya di daerah perkebunan agar diketahui kelompok bakteri rizosfer, sehingga dapat dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mengetahui kelompok dan keragaman bakteri rizosfer pada tanaman sawit yang berumur 8, 11, dan 14 tahun. Penelitian dilakukan dengan pendekatan konvensional dengan karakterisasi morfologi dan uji biokimia. Dari umur-umur tersebut, didapatkan 18 isolat yang terdiri dari 11 genus, yaitu Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, dan Streptococcus. Bakteri rizosfer tanaman sawit memiliki keragaman genus yang sama pada umur 8, 11, dan 14 tahun. Genus bakteri yang didapatkan dapat dimanfaatkan sebagai PGPR (Plant Promoting Rhizobacteria).Abstract Rhizosphere bacteria are bacteria found in root areas. Rhizosphere bacteria have various roles such as providing nutrients for plants, protecting plants from infection pathogenic bacteria, producing growth hormones such as indol acetic acid, phosphate solvents, nitrogen binders, and others. By having these various abilities and roles, it is necessary to explore the bacteria of oil palm plants, especially in the plantation areas so that the group of rhizosphere bacteria can be identified and then utilized optimally. This study aims to determine the group and diversity of rhizosphere bacteria in oil palm plants that are 8, 11, and 14 years old. The research was conducted conventionally by observing morphological and biochemical characterization. At those ages, 18 isolates were consisting of 11 genera, namely Bacillus, Azospirillum, Actinobacillus, Xanthobacter, Enterococcus, Paenibacillus, Klebsiella, Pseudomonas, Arthrobacter, Micrococcus, and Streptococcus through conventional methods. Rhizosphere bacteria in oil palm plants have the same genus diversity at the ages of 8, 11, and 14 years. The bacterial genus obtained can be used as PGPR (Plant Promoting Rhizobacteria).

Insights Into the Molecular Recognition Mechanism of 5-Hydroxy-Indol Acetic Acid by Imprinted Xero-Gel Materials

2013 ◽  
Vol 5 (12) ◽  
pp. 1940-1947 ◽  
Author(s):  
Alejandro Simón de Dios ◽  
Alfonso Fernández-González ◽  
Rosana Badía-Laíño ◽  
Marta Elena Díaz-García
Keyword(s):  
Acetic Acid ◽  
Molecular Recognition ◽  
Recognition Mechanism ◽  
Indol Acetic Acid

scholarly journals Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips

2011 ◽  
Vol 6 (12) ◽  
pp. 2013-2022 ◽  
Author(s):  
Takeshi Nishimura ◽  
Kiminori Toyooka ◽  
Mayuko Sato ◽  
Sachiko Matsumoto ◽  
M. Mercedes Lucas ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Maize Coleoptile ◽  
Indole 3 Acetic Acid

Direct measurement of interphase shortening produced by kinetin plus indol acetic acid in meristematic cells ofAllium cepa L.

1972 ◽  
Vol 28 (2) ◽  
pp. 247-248 ◽  
Author(s):  
A. González-Fernández ◽  
J. L. Díez ◽  
G. Giménez-Martín ◽  
C. de la Torre
Keyword(s):  
Acetic Acid ◽  
Direct Measurement ◽  
Meristematic Cells ◽  
Indol Acetic Acid ◽  
Ofallium Cepa

scholarly journals REGENERASIRUMPUT LAUT Kappaphycus alvarezii (Doty) MELALUI INDUKSI KALUS DAN EMBRIO DENGAN PENAMBAHAN HORMON PERANGSANG TUMBUH SECARA IN VITRO

2009 ◽  
Vol 4 (1) ◽  
pp. 39
Author(s):  
Emma Suryati ◽  
Sri Rejeki Hesti Mulyaningrum
Keyword(s):  
Acetic Acid ◽  
Growth Regulator ◽  
Culture Medium ◽  
Solid Medium ◽  
Agar Medium ◽  
Kappaphycus Alvarezii ◽  
Indol Acetic Acid ◽  
High Quality Seed ◽  
Medium Culture

Regenerasi rumput laut Kappaphycus alvarezii dilakukan dalam rangka penyediaan benih yang bermutu dan mempunyai keunggulan melalui induksi kalus dan embrio dengan penambahan hormon pertumbuhan yang diintroduksi ke dalam media kultur yang dapat memacu induksi kalus dan penebalan pigmen rumput laut. Media kultur yang digunakan adalah media Conwy padat dengan penambahan agar 0,8%-1,6%. Hormon perangsang tumbuh yang digunakan untuk memacu pertumbuhan kalus dan filamen embrio yaitu IAA (Indol acetic acid), kinetin, dan auxilin dengan konsentrasi berkisar 0,4-1 mg/L. Embrio yang dihasilkan merupakan anakan yang mempunyai sifat yang sama dengan induknya. Sintasan dan perkembangan embrio yang paling baik yaitu dengan penambahan IAA dengan konsentrasi 0,4 mg/L pada media padat. Pembentukan anakan dilakukan dengan mengiris embrio dan menumbuhkan pada media cair yang diperkaya dengan hormon yang sama. Pemeliharaan anakan pada media kultur dilakukan hingga mencapai ukuran 2-3 cm.Regeneration of seaweed Kappaphycus alvarezii was done to provide high quality seed through callus and embryo induction using plant growth regulator which was introducted to the culture medium. This growth regulator can stimulate the callus induction procces and thickening the seaweed pigment. Applied medium culture was agar medium with 0.8%-1.6% concentration enriched with Conwy and the applied growth regulators were IAA (Indol acetic acid), kinetin dan auxilin with 0.4-1 mg/L concentration range. Resulted embryo has the same characteristics with the stock. The best survival rate and embryo growth was IAA treatment with 0.4 mg/L concentration. Formation of embryo was done by transferring them from solid medium to the liquid one with the same growth regulator treatment. The nursery of the seed in culture medium was carried out until it has reached 2-3 cm in size.

scholarly journals Possible roles of both abscisic acid and indol-acetic acid in controlling grape berry ripening process

OENO One ◽  
2007 ◽  
Vol 41 (3) ◽  
pp. 141 ◽  
Author(s):  
Christelle Deytieux-Belleau ◽  
Séverine Gagne ◽  
Annie L'Hyvernay ◽  
Bernard Donèche ◽  
Laurence Geny
Keyword(s):  
Acetic Acid ◽  
Abscisic Acid ◽  
Plant Growth ◽  
Growth Regulators ◽  
Grape Berry ◽  
Ripening Process ◽  
Hormonal Profile ◽  
Indol Acetic Acid ◽  
Berry Ripening ◽  
Grape Berry Ripening

<p style="text-align: justify;"><strong>Aims</strong>: The objective was to better understand the mechanisms involved in grape ripening that brings about important changes in the physiology and chemistry of the fruit. So we focused on the involvement of two growth regulators: abscisic acid (ABA) and indol-acetic acid (IAA) in controlling grape berry ripening process.</p><p style="text-align: justify;"><strong>Methods and results</strong>: We described the evolution of the two plant growth regulators during the development of cv. Merlot grapes (Vitis vinifera L.). In order to better understand the role of ABA and IAA in the ripening control, these two growth regulators were applied on the grapes at the onset of veraison. The hormonal profile was established on treated berries and different physiological parameters were assayed to evaluate the effects of both applications. The partitioning of both plant growth regulators in nontreated berries showed that ABA and IAA accumulated at the onset of ripening. Moreover, it appeared that endogenous ABA decreased progressively in the flesh while accumulated in the skin from the beginning of the colour change to maturity. The hormonal treatments modified the hormonal profile and several physiological parameters: sugar, acidity, colour, and Botrytis sensibility.</p><p style="text-align: justify;"><strong>Conclusion</strong>: These findings suggest that both treatments have modified the ripening process. Exogenous ABA has induced advancement in grape ripening, while IAA application has delayed this process. These observations support the view that the grape ripening process may be influenced by the hormonal status.</p><p style="text-align: justify;"><strong>Significance and impact of study</strong>: This study gives new information about the ripening control of the non-climacteric fruits. In grape berries, it provides evidence of a possible co-involvement of ABA and IAA in controlling ripening process.</p>

scholarly journals Estimation levels of Indol acetic acid (IAA) and Gibberellic acid (GA3) from dry bakery yeast Saccharomyces cereviciae

2010 ◽  
Vol 4 (2) ◽  
pp. 94-100
Author(s):  
Arwa A Tawfiq
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Indol Acetic Acid ◽  
Bakery Yeast

The level of endogenous free, bound and total auxin (Indol-3-acitic acid, IAA) and gibberellic acid (GA3) were examined in dry bakery yeast. For determination of the levels of these plant growth regulators level, used spectrophotometer. The results show that Saccharomyces cereviciae was produced IAA and GA3. IAA level was 29.86 µg/ml, 198 µg/ml at 222nm and 280nm wavelengths respectively and GA3 level was 799 µg/ml at 254nm.

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