Production de composés indoliques rhizogènes par le ver de terre Lumbricus terrestris

2001 ◽  
Vol 79 (11) ◽  
pp. 1921-1932 ◽  
Author(s):  
Abdellatif El Harti ◽  
Mohamed Saghi ◽  
J -AE Molina ◽  
Gérard Teller
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Lumbricus Terrestris ◽  
Polar Group ◽  
Bean Plants ◽  
Indol Acetic Acid ◽  
Layer Chromatography ◽  
Group Of Fractions

In vitro application of total gross extract of earthworms (Lumbricus terrestris) in diverse dilutions stimulates rhizogenesis in young bean plants (Phaseolus vulgaris). The observed effect is similar to that of indol acetic acid, a well-known growth enhancer in plants, used here as a control in various concentrations. Fragmentation of worm extract by column chromatography results in three groups of fractions. Only the polar group of fractions has a significant rhizogenous effect, which is, however, inferior to that observed in the presence of total gross extract of worms or of indol acetic acid. Gross extract analyses using thin layer chromatography, with appropriate chromatography systems and reagents, revealed that indol acetic acid is not present, but is probably replaced by other indol-derived substances that have a neutral to basic chromatographic behaviour. These presumed indol-derived substances are identified as methyl-tryptophane, serotonin, and hydroxy-indol acetic acid. Analyses using mass spectrometry combined with gas chromatography, following fragmentation and purification of the group of rhyzogenous fractions, have revealed the presence of hydroxy-indol carboxylic acid, which seems to take the form of several isomeres.[Journal translation]

Production d'une substance rhizogène à effet similaire à celui de l'acide indole acétique par le ver de terre Lumbricus terrestris

2001 ◽  
Vol 79 (11) ◽  
pp. 1911-1920 ◽  
Author(s):  
Abdellatif El Harti ◽  
Mohamed Saghi ◽  
J -AE Molina ◽  
Gérard Teller
Keyword(s):  
Acetic Acid ◽  
Phaseolus Vulgaris ◽  
Root Growth ◽  
Digestive Tract ◽  
Lumbricus Terrestris ◽  
Indol Acetic Acid ◽  
Biological Tests ◽  
Exogenous Origin

In vitro biological tests show that excreta or gross total and partial extracts of Lumbricus terrestris stimulate rhizogenesis and enhance root growth in young plants of the bean Phaseolus vulgaris. Similar results were obtained in experiments with worms freshly collected in the field and with worms previously deprived of food for 4 weeks. The rhizogenous substance produced by the worms is therefore not of exogenous origin, coming from the soil via the digestive tract. The similar effects of indol acetic acid at different concentrations and of excreta and gross extracts of worms in various dilutions indicate that the rhizogenous substance is similar to indol acetic acid, a well-known phytohormone in plants. Expressed as indol acetic acid equivalents, the quantity of the rhizogenous substance in worms would be approximately 18 × 103 ng/g, of which half (9 × 103 ng/g) is released in the excreta alone.[Journal translation]

scholarly journals Identifying volatile in vitro biomarkers for oral bacteria with proton-transfer-reaction mass spectrometry and gas chromatography–mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kajsa Roslund ◽  
Markku Lehto ◽  
Pirkko Pussinen ◽  
Kari Hartonen ◽  
Per-Henrik Groop ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Proton Transfer ◽  
Transfer Reaction ◽  
Proton Transfer Reaction ◽  
Oral Bacteria ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Analysis ◽  
Bacterial Volatiles

AbstractWe have measured the volatile fingerprints of four pathogenic oral bacteria connected to periodontal disease and dental abscess: Porphyromonas gingivalis (three separate strains), Prevotella intermedia, Prevotella nigrescens and Tannerella forsythia. Volatile fingerprints were measured in vitro from the headspace gas of the bacteria cultured on agar. Concrete identification of new and previously reported bacterial volatiles were performed by a combination of solid phase microextraction (SPME) and offline gas chromatography–mass spectrometry (GC–MS). We also studied the effect of the reduced electric field strength (E/N) on the fragmentation patterns of bacterial volatiles in online proton-transfer-reaction time-of-flight mass spectrometry (PTR-ToF-MS). We aimed to discover possible new biomarkers for the studied oral bacteria, as well as to validate the combination of GC–MS and PTR-MS for volatile analysis. Some of the most promising compounds produced include: 1-Methyl-1,2,3,4-tetrahydroisoquinoline (1MeTIQ), indole, and a cascade of sulphur compounds, such as methanethiol, dimethyl disulphide (DMDS) and dimethyl trisulphide (DMTS). We also found that several compounds, especially alcohols, aldehydes and esters, fragment significantly with the PTR-MS method, when high E/N values are used. We conclude that the studied oral bacteria can be separated by their volatile fingerprints in vitro, which could have importance in clinical and laboratory environments. In addition, using softer ionization conditions can improve the performance of the PTR-MS method in the volatile analysis of certain compounds.

Thin-layer chromatography and gas chromatography-mass spectrometry examination of shoe materials from patients with shoe dermatitis

2015 ◽  
Vol 72 (4) ◽  
pp. 248-252 ◽  
Author(s):  
Sri A. Febriana ◽  
Erik Zimerson ◽  
Cecilia Svedman ◽  
Winarto Haryadi ◽  
Pieter-Jan Coenraads ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Layer Chromatography

Gas chromatography-mass spectrometry fingerprint and in vitro cytotoxic studies of Rubus steudneri leaf fractions against michigan cancer foundation-7 breast cancer cell line

2021 ◽  
Vol 17 (5) ◽  
pp. 54
Author(s):  
RaghavendraLakshmana Shetty Hallur ◽  
ChaitanyaV. N L. Motamarri ◽  
PrashithKekuda T. Ramamoorthy ◽  
ChetanD Murthy ◽  
RavikumarPatil H. Siddappa ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Cell Line ◽  
Breast Cancer Cell ◽  
Breast Cancer Cell Line ◽  
Cancer Cell Line ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytotoxic Studies

scholarly journals GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF AN ENDANGERED MEDICINAL PLANT, SARCOSTEMMA VIMINALE (L.) R.BR. FROM THAR DESERT, RAJASTHAN (INDIA)

2017 ◽  
Vol 10 (9) ◽  
pp. 210 ◽  
Author(s):  
Arora Sunita ◽  
Meena Sonam ◽  
Kumar Ganesh
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Acetic Acid ◽  
Medicinal Plants ◽  
Maximum Amount ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
World Population ◽  
Thar Desert ◽  
Chromatography Mass Spectrometry

  Objective: Sarcostemma viminale (L.) R. Br. is one of the important endangered medicinal plants belonging to the family Asclepiadaceae. The aim of the present investigation was to determine the possible bioactive phytochemicals from stem of S. viminale (L.) R. Br. using methanol, chloroform, and hexane as solvents.Methods: Plant material was collected from typical conditions of Indian Thar Desert in the month of July-September, 2016. This plant always grows in association with the congeneric plant, Euphorbia caducifolia. The phytochemical compounds were investigated using Perkin-Elmer gas chromatography-mass spectrometry, while the mass spectra of the compounds found in the extract were matched with the National Institute of Standards and Technology library.Results: Maximum % area is found for Lup-20-(29)-en-3-yl acetate is present maximum amount (40.85%) with reaction time (RT)=43.787 minutes, followed by 4, 4, 6A, 6B, 8A, 11, 11, 14B-octamethyl-1, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-octadecahydro-2H-picen-3- one$$olean-12-en-3-one# (13.74%) with RT=44.420 minutes in the methanolic extract; acetic acid 4, 4, 6A, 8A, 11, 12, 14B-octamethyl-1, 2, 3, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-eicosahydro-picen-3-yl ester $$ urs-12-en-3-yl acetate is present maximum amount (44.98%) with RT=48.265 minutes, followed by. beta.-amyrin (18.51%) with RT=40.580 minutes in the chloroform extract; acetic acid 4, 4, 6A, 8A, 11, 12, 14B-octamethyl-1, 2, 3, 4, 4A, 5, 6, 6A, 6B, 7, 8, 8A, 9, 10, 11, 12, 12A, 14, 14A, 14B-eicosahydro-picen-3-yl ester $$ urs-12-en-3-yl acetate is present maximum amount (45.47%) with RT=48.514 minutes, followed by. beta.-amyrin (19.21%) with RT=40.555 minutes in the hexane extract of stem of S. viminale (L.) R. Br.Conclusion: Medicinal plants contain one or more substances that can be used for therapeutic purpose; they are used by the world population for their basic health needs. The importance of the study is to investigate the pinpoint biological activity of some of these compounds so that they can be used by pharma or some other drug designing industry to find a novel drug.

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