An Amino Acid Substitution in the QB-Protein Causes Herbicide Resistance without Impairing Electron Transport from QA to QB
1989 ◽
Vol 44
(5-6)
◽
pp. 431-434
◽
Keyword(s):
Abstract Structure and function of the QB-protein of a metribuzin resistant mutant of Chlamydomonas reinhardii were analyzed. The amino acid residue Leu-275 of the wild type protein is changed to Phe as was determined by RNA -sequence analysis. This mutation caused a 20-fold and 5-fold resistance to metribuzin and DCMU , respectively. No resistance to atrazine was observed. The kinetics of the electron transport from QA to OB was similar to that of the wild type (t1/2 = 0.4 ms).