Downstream Processing for Xylitol Recovery from Fermented Sugar Cane Bagasse Hydrolysate Using Aluminium Polychloride

Abstract Xylitol, a sweetener comparable to sucrose, is anticariogenic and can be consumed by diabetics. This sugar has been employed sucessfully in many foods and pharmaceutical products. The discovery of microorganisms capable of converting xylose present in lignocellulosic biomass into xylitol offers the opportunity of producing this poliol in a simple way. Xylitol production by biotechnological means using sugar cane bagasse is under study in our laboratories, and fermentation parameters have already been established. However, the downstream processing for xylitol recovery is still a bottleneck on which there is only a few data available in the literature. The present study deals with xylitol recovery from fermented sugar cane bagasse hydrolysate using 5.2 g/l of aluminium polychloride associated with activated charcoal. The experiments were performed at pH 9, 50 °C for 50 min. The results showed that aluminium polychloride and activated charcoal promoted a 93.5% reduction in phenolic compounds and a 9.7% loss of xylitol from the fermented medium , which became more discoloured, facilitating the xylitol separation.

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Effects of different sources of forage in high-concentrate diets on fermentation parameters, ruminal biohydrogenation and microbiota in Nellore feedlot steers

The Journal of Agricultural Science ◽

10.1017/s0021859616000204 ◽

2016 ◽

Vol 154 (5) ◽

pp. 928-941 ◽

Cited By ~ 2

Author(s):

J. D. MESSANA ◽

A. L. E. G. F. CARVALHO ◽

A. F. RIBEIRO ◽

G. FIORENTINI ◽

P. S. CASTAGNINO ◽

...

Keyword(s):

Sugar Cane ◽

Protein Digestibility ◽

Ammonia Nitrogen ◽

Sugar Cane Bagasse ◽

The Other ◽

Selenomonas Ruminantium ◽

Diet Formulation ◽

Fermentation Parameters ◽

Ruminal Biohydrogenation ◽

Different Sources

SUMMARYModifying the extent of fatty acid (FA) biohydrogenation (BH) in the rumen through diet formulation is an effective strategy for changing the content of unsaturated FAs (USFAs) in meat. The present study investigated the effects of different sources of forage in high-concentrate diets on intake, digestibility, rumen fermentation, ruminal BH, duodenal flow of FAs and rumen microbiota in Nellore steers. Intake of neutral detergent fibre (NDF) was higher in animals fed with maize silage (MS) than in those fed with sugar cane (SC) and sugar cane bagasse (SB). Higher digestibility of dry matter and NDF was found in animals fed with MS than in those fed with the other diets. In addition, higher crude protein digestibility was observed in animals fed with sugar cane bagasse than in those fed with SC. Non-fibre carbohydrate (NFC) digestibility was higher in animals fed with sugar cane than in those fed with the other diets. Intake of total and individual FAs such as C18 : 1cis-9, C18 : 2, and C18 : 3 was similar between animals fed with MS and SB, but decreased in animals fed with SC. Diets containing MS and SB showed higher total digestibility of saturated FAs (SFAs) and USFAs, respectively and total FAs and ruminal BH of C18 : 1 and USFA. Intestinal digestibility of overall FAs did not differ among treatments, except for C18 : 3, which increased in animals fed with SC and SB. The profile of FAs in duodenal digesta and faecal outputs did not differ among treatments. However, the flow of NDF was higher in animals fed with SC than in those fed with MS and SB. Animals fed with SB showed higher values of pH than those fed with MS and SC. Animals fed with SC showed lower values of ammonia-nitrogen. Protozoan counts were only influenced by diet for species that belonged to the generaDasytrichaandIsotricha. Populations of fibrolytic bacteria (Ruminococus flavefaciens, Ruminococus albusandFibrobacter succinogenes) were similar among diets. Populations ofSelenomonas ruminantiumincreased 2·5 and 5 times in animals fed with MS when compared with those fed with SC and SB, respectively. The use of MS increased intake and digestibility of NDF, and the use of SC decreased ruminal BH of total USFA without changing the flow of FAs to the duodenum. Thus, different sources of forage in high-concentrate diets do not modify the duodenal flow of USFA or fibrolytic bacteria. This must be taken into account when formulating diets to modulate ruminal upsets without altering intake.

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Fed-batch culture of Candida guilliermondii FTI 20037 for xylitol production from sugar cane bagasse hydrolysate

Letters in Applied Microbiology ◽

10.1046/j.1472-765x.1999.00639.x ◽

1999 ◽

Vol 29 (6) ◽

pp. 359-363 ◽

Cited By ~ 15

Author(s):

D. C. G. A. Rodrigues ◽

S. S. da Silva ◽

M. G. A. Felipe

Keyword(s):

Batch Culture ◽

Sugar Cane ◽

Candida Guilliermondii ◽

Sugar Cane Bagasse ◽

Xylitol Production ◽

Fed Batch ◽

Fed Batch Culture

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Environmental parameters affecting xylitol production from sugar cane bagasse hemicellulosic hydrolyzate by Candida guilliermondii

Journal of Industrial Microbiology & Biotechnology ◽

10.1038/sj.jim.2900374 ◽

1997 ◽

Vol 18 (4) ◽

pp. 251-254 ◽

Cited By ~ 37

Author(s):

M G A Felipe ◽

M Vitolo ◽

I M Mancilha ◽

S S Silva

Keyword(s):

Sugar Cane ◽

Environmental Parameters ◽

Candida Guilliermondii ◽

Sugar Cane Bagasse ◽

Xylitol Production

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Experimental determination of the effects of pretreatment on selected Nigerian lignocellulosic biomass in bioethanol production

Scientific Reports ◽

10.1038/s41598-020-78105-8 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Adeolu A. Awoyale ◽

David Lokhat

Keyword(s):

Lignocellulosic Biomass ◽

Sugar Cane ◽

Sugar Cane Bagasse ◽

Crystalline Cellulose ◽

Cellulose Content ◽

Bioethanol Production ◽

Rice Husks ◽

Pore Characteristics ◽

Corn Cobs ◽

Cassava Peels

AbstractIn the present study, five lignocellulosic biomass namely, corn cobs (Zea mays), rice husks (Oryza sativa), cassava peels (Manihot esculenta), sugar cane bagasse (Saccharum officinarum), and white yam peels (Dioscorea rotundata) of two mesh sizes of 300 and 425 microns and a combination of some and all of the biomass were pretreated using combined hydrothermal and acid-based, combined hydrothermal and alkali-based and hydrothermal only processes. The raw and pretreated biomass were also characterized by Fourier transform infrared spectroscopy (FT-IR), Brunauer–Emmett–Teller (BET), X-Ray diffraction (XRD), and Scanning electron microscopy (SEM) to determine the effects of the various pretreatments on the biomass being studied. The cellulose values of the raw biomass range from 25.8 wt% for cassava peels biomass to 40.0 wt% for sugar cane bagasse. The values of the cellulose content increased slightly with the pretreatment, ranging from 33.2 to 43.8 wt%. The results of the analysis indicate that the hydrothermal and alkaline-based pretreatment shows more severity on the different biomass being studied as seen from the pore characteristics results of corn cobs + rice husks biomass, which also shows that the combination of feedstocks can effectively improve the properties of the biomass in the bioethanol production process. The FTIR analysis also showed that the crystalline cellulose present in all the biomass was converted to the amorphous form after the pretreatment processes. The pore characteristics for mixed corn cobs and rice husks biomass have the highest specific surface area and pore volume of 1837 m2/g and 0.5570 cc/g respectively.

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