Instability of Three-Dimensional Structures in Ribosomal Cores Evidenced by Microcalorimetric Studies

2000 ◽  
Vol 55 (5-6) ◽  
pp. 410-412 ◽  
Author(s):  
Adalberto Bonincontro ◽  
Stefania Cinelli ◽  
Giuseppe Onori ◽  
Gianfranco Risuleo

In this paper we show a microcalorimetric investigation carried out on the so-called cores, i. e. ribosomes deprived of select proteins by LiCl treatment. Thermal degradation of native ribosomes gives rise to two thermal transitions occurring at different temperatures. In the cores the high temperature peak persists even after treatment at very high ion strength (2 м LiCl). This strongly suggests the existence of a very stable structure that was previously observed also in particles treated with agents that hydrolyze the RNA moiety. The low temperature peak gradually but dramatically decreases even though it never disappears completely This indicates that the treatment to obtain ribosomal cores does not cause complete unfolding of the particle but only the destabilization of a structural three-dimensional domain present in native ribosomes. These data are discussed in the light of previous results obtained by dielectric spectroscopy and microcalorimetric studies on ribosomal particles.

2019 ◽  
Vol 32 (1) ◽  
pp. 143-151 ◽  
Author(s):  
Luma Rayane de Lima Nunes ◽  
Paloma Rayane Pinheiro ◽  
Charles Lobo Pinheiro ◽  
Kelly Andressa Peres Lima ◽  
Alek Sandro Dutra

ABSTRACT Salinity is prejudicial to plant development, causing different types of damage to species, or even between genotypes of the same species, with the effects being aggravated when combined with other types of stress, such as heat stress. The aim of this study was to evaluate the tolerance of cowpea genotypes (Vigna unguiculata L. Walp.) to salt stress at different temperatures. Seeds of the Pujante, Epace 10 and Marataoã genotypes were placed on paper rolls (Germitest®) moistened with different salt concentrations of 0.0 (control), 1.5, 3.0, 4.5 and 6.0 dS m-1, and placed in a germination chamber (BOD) at temperatures of 20, 25, 30 and 35°C. The experiment was conducted in a completely randomised design, in a 3 × 4 × 5 scheme of subdivided plots, with four replications per treatment. The variables under analysis were germination percentage, first germination count, shoot and root length, and total seedling dry weight. At temperatures of 30 and 35°C, increases in the salt concentration were more damaging to germination in the Epace 10 and Pujante genotypes, while for the Marataoã genotype, damage occurred at the temperature of 20°C. At 25°C, germination and vigour in the genotypes were higher, with the Pujante genotype proving to be more tolerant to salt stress, whereas Epace 10 and Marataoã were more tolerant to high temperatures. Germination in the cowpea genotypes was more sensitive to salt stress when subjected to heat stress caused by the low temperature of 20°C or high temperature of 35°C.


2014 ◽  
Vol 1039 ◽  
pp. 107-111
Author(s):  
Yang Chen ◽  
Gui Qin Li ◽  
Bin Ruan ◽  
Xiao Yuan ◽  
Hong Bo Li

The mechanical behavior of plastic material is dramatically sensitive to temperature. An method is proposed to predict the mechanical behavior of plastics for cars, ranging from low-temperature low temperature ≤-40°C to high temperature ≥80°C. It dominates the behavior of plastic material based on improved constitutive model in which the parameters adjusted by a series of tests under different temperatures. The method is validated with test and establishes the basis for research and development of plastic parts for automobile as well.


Parasitology ◽  
2015 ◽  
Vol 142 (10) ◽  
pp. 1290-1296 ◽  
Author(s):  
SIRPA KAUNISTO ◽  
LAURA HÄRKÖNEN ◽  
MARKUS J. RANTALA ◽  
RAINE KORTET

SUMMARYImmunity of parasites has been studied amazingly little, in spite of the fact that parasitic organisms, especially the arthropod parasites, need immunity to survive their own infections to successfully complete life cycles. Long-term effects of challenging environmental temperatures on immunity have remained unstudied in insects and parasites. Our study species, the deer ked (Lipoptena cervi; Linnaeus 1758), is an invasive, blood-feeding parasitic fly of cervids. Here, it was studied whether thermal stress during the pupal diapause stage could modify adult immunity (encapsulation capacity) in L. cervi. The effect of either a low temperature or high temperature peak, experienced during winter dormancy, on encapsulation response of active adult was tested. It was found that low temperature exposure during diapause, as long as the temperature is not too harsh, had a favourable effect on adult immunity. An abnormal, high temperature peak during pupal winter diapause significantly deteriorated the encapsulation capacity of emerged adults. The frequency and intensity of extreme weather events such as high temperature fluctuations are likely to increase with climate change. Thus, the climate change might have previously unknown influence on host-ectoparasite interactions, by affecting ectoparasite's immune defence and survival.


2006 ◽  
Vol 319 ◽  
pp. 151-156 ◽  
Author(s):  
Y. Hiki ◽  
M. Tanahashi ◽  
Shin Takeuchi

In a hydrogen-doped metallic glass, there appear low-temperature and high-temperature internal friction peaks respectively associated with a point-defect relaxation and the crystallization. The high-temperature-side slope of low-temperature peak and also the low-temperature-side slope of high-temperature peak enhance the background internal friction near the room temperature. A hydrogen-doped Mg-base metallic glass was proposed as a high-damping material to be used near and somewhat above the room temperature. Stability of the high damping was also checked.


Author(s):  
Robert H. Swendsen

The main application of Fermi–Dirac Statistics is to calculate the properties of electrons. This chapter explains how the properties of fermions account for the behavior of metals. The Fermi energy is introduced and shown to correspond to a very high temperature, so that most properties can be obtained from low-temperature expansions. Both discrete and continuous densities of states are discussed. The Sommerfeld expansion is derived explicitly. The low-temperature specific heat and compressibility are derived. The most important fermions are electrons, and understanding the properties of electrons is central to understanding the properties of all materials. In this chapter we will study the ideal Fermi gas, which turns out to explain many of the properties of electrons in metals.


1992 ◽  
Vol 270 ◽  
Author(s):  
Zdenék Slanina ◽  
Ludwik Adamowicz

ABSTRACTPurely carbonaceous aggregates C20 have been studied by the AM1 quantumchemical method. In addition to one dodecahedron-shaped structure possessing C1 symmetry another three-dimensional species is revealed, viz. a bowl-shaped structureof C5v symmetry (and also one two-dimensional and two one-dimensional species). Temperature dependence of the relative stabilities of both three-dimensional structures is evaluated, showing that in the relevant temperature region the fullerenic species is prevailing. However, in a very high temperature region a relative-stability interchange has been predicted.


2001 ◽  
Vol 16 (11) ◽  
pp. 2009-2014 ◽  
Author(s):  
MASSIMO CAMPOSTRINI ◽  
PAOLO ROSSI ◽  
ETTORE VICARI ◽  
MARTIN HASENBUSCH ◽  
ANDREA PELISSETTO

Three-dimensional spin models of the Ising and XY universality classes are studied by a combination of high-temperature expansions and Monte Carlo simulations applied to improved Hamiltonians. The critical exponents and the critical equation of state are determined to very high precision.


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