scholarly journals Outdoor and Indoor Cultivation of Spirulina platensis in the Extreme South of Brazil

2008 ◽  
Vol 63 (1-2) ◽  
pp. 85-90 ◽  
Author(s):  
Michele R. Andrade ◽  
Jorge A. V. Costa
Keyword(s):  
Growth Rate ◽  
Spirulina Platensis ◽  
Doubling Time ◽  
Specific Growth ◽  
Biomass Concentration ◽  
Maximum Specific Growth Rate ◽  
Day Length ◽  
Controlled Conditions ◽  
Dry Biomass ◽  
South Of Brazil

Water supplemented with 10% or 20% (v/v) of Zarrouk medium was used to cultivate Spirulina platensis in closed and open bioreactors under controlled conditions (30 °C, 32.5 μmol m−2 s−1, 12 h light/dark photoperiod) and in a greenhouse (9.4 to 46 °C, up to 2800 μmol m−2 s−1, variable day length photoperiod) using different initial biomass concentrations (X0) in the extreme south of Brazil (32.05° S, 52.11° W). Under controlled conditions the maximum specific growth rate (μmax) was 0.102 d−1, the biomass doubling time (td) was 6.8 d, the maximum dry biomass concentration (Xmax) was 1.94 g LD1 and the maximum productivity (Pmax) was 0.059 g L−1 d−1, while the corresponding values in the greenhouse experiments were μmax = 0.322 d−1, td = 2.2 d, Xmax = 1.73 g L−1 and Pmax = 0.112 g L−1 d−1. Under controlled conditions the highest values for these parameters occurred when X0 = 0.15 g L−1, while in the greenhouse X0 = 0.4 g L−1 produced the highest values. These results show that the cultivation of S. platensis in greenhouses in the extreme south of Brazil is technically viable and that the S. platensis inoculum and the concentration of Zarrouk medium can be combined in such a way as to obtain growth and productivity parameters comparable, or superior, to those occurring in bioreactors under controlled conditions of temperature, illuminance and photoperiod.

scholarly journals Pengaruh Komposisi Air Limbah Industri Kertas sebagai Media Pertumbuhan dan Fotoperiode Terhadap Kultur Spirulina platensis

2019 ◽  
Vol 9 (01) ◽  
pp. 9
Author(s):  
Meisa Legi Rizkiana ◽  
Saepulloh Saepulloh ◽  
Prima Besty Asthary ◽  
Rahmaniar Mulyani
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Flue Gas ◽  
Spirulina Platensis ◽  
Doubling Time ◽  
Specific Growth ◽  
Paper Industry ◽  
Biomass Concentration ◽  
Treated Wastewater ◽  
Wet Scrubber

Industri kertas menghasilkan air limbah dari kegiatan produksi dan proses flue gas desulphurization (air limbah wet scrubber). Senyawa nutrisi (senyawa karbon, nitrogen, dan fosfat) yang masih terdapat dalam air limbah terolah dan air limbah wet scrubber belum dimanfaatkan secara optimal dan berpotensi sebagai media pertumbuhan Spirulina platensis. Penelitian dengan variasi komposisi air limbah dan fotoperiode (24 jam terang dan 12 jam terang/12 jam gelap) telah dilakukan dalam skala laboratorium selama 9 hari. Pengukuran pH media, pertumbuhan S. platensis (konsentrasi biomassa, laju pertumbuhan spesifik, dan waktu penggandaan), dan kandungan fikosianin dilakukan setiap hari untuk melihat pengaruh komposisi media dan perlakuan fotoperiode terhadap pertumbuhan S. platensis. Hasil menunjukkan bahwa S. platensis dapat tumbuh dengan baik pada semua komposisi air limbah terolah dan air limbah wet scrubber yang diujicobakan. Konsentrasi biomassa tertinggi sebesar 831 mg/L dan laju pertumbuhan spesifik tertinggi sebesar 0,52 hari-1 dengan waktu penggandaan 0,4 hari diperoleh dari perlakuan fotoperiode 24 jam terang. Komposisi air limbah terolah dan air limbah wet scrubber tidak memberikan pengaruh yang nyata terhadap konsentrasi biomassa, tetapi perbedaan fotoperiode sangat berpengaruh terhadap konsentrasi biomassa sehingga dalam aplikasi skala lapangan perlu mempertimbangkan sistem pencahayaan untuk pertumbuhan S. platensis yang optimal.Kata kunci: Spirulina platensis, media pertumbuhan, fotoperiode, air limbah terolah, air limbah wet scrubberThe Effect of Composition of Paper Industry Wastewater as A Growth Medium and Photoperiod on The Culture of Spirulina platensisAbstractThe paper industry produces wastewater from its production activities and the flue gas desulphurization process (wet scrubber wastewater). Nutritional compounds (carbon, nitrogen and phosphate compounds) which are still in treated wastewater and wet scrubber wastewater have not been utilized optimally and have potential as growth media of Spirulina platensis. Research on wastewater compositions and photoperiod variations (24 hours light and 12 hours light/12 hours dark) was carried out on a laboratory scale for 9 days. Measurements of medium pH, growth of S. platensis (biomass concentration, specific growth rate, and doubling time), and phycocyanin content were carried out every day to study the effect of wastewater composition and photoperiod treatment on the growth of S. platensis. The results showed that S. platensis can grow well on all compositions of treated wastewater and wet scrubber wastewater. The highest biomass concentration of 831 mg/L and the highest specific growth rate of 0.52 days-1 with a doubling time of 0,4 days is obtained from 24-hour light photoperiod treatment. The composition of treated wastewater and wet scrubber wastewater has no significant effect on the biomass concentration but the photoperiod differences greatly influence the biomass concentration so that in the field scale application it is necessary to consider the lighting system for optimal S. platensis growth. Keywords:  Spirulina platensis, growth medium, photoperiod, treated wastewater, wet scrubber wastewater

Growth Kinetic of Fresh and Frezee-Dried Pleurotus sajor-caju (Oyster Mushroom) Mycelium for Preservation Study

2013 ◽  
Vol 594-595 ◽  
pp. 196-202
Author(s):  
Zarina Zakaria ◽  
Shi Fern Chong ◽  
Amirul Rasyid Zahari ◽  
Nurul Aina Fauzi ◽  
Sharul Aida Mohd Shayuti
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Doubling Time ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Oyster Mushroom ◽  
Solid Media ◽  
Effective Media ◽  
Freeze Dried ◽  
Media Condition

An investigation was conducted to determine an effective media condition for the growth of fresh and freeze-dried oyster mushroom mycelium. Fresh and freeze-dried mycelium sub-culture on liquid media obtained 0.3022 and 0.5081 maximum specific growth rate respectively. The doubling time for fresh and freeze-dried mycelium was calculated 2.2937 and 1.3642 correspondingly. On solid media, maximum specific growth rate for fresh and freeze-dried mycelium was obtained lower at 0.1636 and 0.3719 respectively. The doubling time for fresh and freeze-dried however, was calculated higher at 4.2368 and 1.8683 correspondingly. Freeze dried mycelium showed good recovery tendency after preservation at low temperature.

Growth Kinetic Study on Lyophilized and Cryopreserved Pleurotus sajor-caju Spawn

2015 ◽  
Vol 754-755 ◽  
pp. 1076-1080
Author(s):  
Sharul Aida Mohd Shayuti ◽  
Shi Fern Chong ◽  
Zarina Zakaria ◽  
Dachyar Arbain ◽  
Noorulnajwa Diyana Yaacob
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Doubling Time ◽  
Specific Growth ◽  
Maximum Growth ◽  
Maximum Growth Rate ◽  
Maximum Specific Growth Rate ◽  
High Survival ◽  
Significant Difference ◽  
Preservation Technique

A study was conducted to determine the most optimal preservation technique for P. sajor-caju spawns which produce maximum specific growth rate and shortest doubling time by using contois kinetic model. The analyzed experimental data showed that lyophilized P. sajor-caju spawn showed the highest maximum specific growth rate, and shortest doubling time compared to cryopreserved P. sajor-caju spawn and 4oC stored P. spawn. There was no significant difference in aspect of growth rate between the lyophilization and cryopreservation techniques which were; 0.148 (μmax)/ (g/day) and 0.147(μmax)/ (g/day) respectively. Based on the result, lyophilization technique was considered as the best preservation technique for preserving P. sajor-caju spawn due to high maximum growth rate which indicates high survival after exposure to preservation treatment.

scholarly journals Growth performance and nutrition value of Spirulina sp. under different photoperiod

2010 ◽  
Vol 9 (2) ◽  
pp. 146 ◽  
Author(s):  
Tatag Budiardi ◽  
Nur Bambang Priyo Utomo ◽  
Asep Santosa
Keyword(s):  
Growth Rate ◽  
Population Density ◽  
Specific Growth Rate ◽  
Doubling Time ◽  
Production Efficiency ◽  
Specific Growth ◽  
Maximum Growth ◽  
Dry Biomass ◽  
Nutrition Value ◽  
Photoperiod Manipulation

<p>This study was conducted to analyze the production of freshwater Spirulina sp. cultured with photoperiod manipulation treatment. In this study, photoperiod manipulation treatment performed on cultured spirulina using fiber tanks (100 L). Spirulina was grown with different photoperiod (bright/T and dark/G) that are six hours per day (6T-18G), 12 hours per day (12T-12G), 18 hours per day (18T-6G), and 24 hours per day (24L-0G). The parameters were observed include dry biomass, population density (N), specific growth rate (SGR), doubling time (G), proximate analysis, and water quality. The results of this study showed that the optimum population density was achieved on day-3 days of cultured, and manipulation photoperiod showed no significant effect to the dry biomass harvest and population density, but significantly affect the specific growth rate and doubling time. Treatment of lighting 12, 18 and 24 hours per day to produce the maximum specific growth rate (0,345 to 0,366 per day) and a maximum doubling time (1,89 to 2,01 days) were not significantly different, whereas the old treatment six hours per day lighting showed the lowest maximum growth rate (0,323 per day) and highest doubling time (2,15 days). At treatment of lighting 12 hours per day, relatively higher protein content (39,73%) than others. In conclusion, the lighting 12 hours per day resulted in optimum production efficiency than other treatments.<br /><br />Keywords: Spirulina sp., photoperiod, density, biomass, growth, nutrition value.</p><p> </p><p><strong>ABSTRAK</strong><br /><br />Penelitian ini dilakukan untuk menganalisis produksi spirulina Spirulina sp. air tawar yang dikultur dengan manipulasi fotoperiode.  Dalam penelitian ini, spirulina dikultur dalam wadah fiber 100 L dengan perlakuan fotoperiode (terang/T dan gelap/G) berbeda, yaitu enam jam per hari (6T-18G), 12 jam per hari (12T-12G), 18 jam per hari (18T-6G), dan 24 jam per hari (24T-0G). Parameter yang diamati meliputi biomassa kering, kepadatan populasi (N), laju pertumbuhan spesifik (SGR), waktu penggandaan (G), dan analisis proksimat sprirulina, serta kualitas air. Hasil penelitian menunjukkan bahwa kepadatan populasi optimum dicapai pada hari ke-3 umur kultur dan manipulasi fotoperiode tidak berpengaruh nyata terhadap hasil biomassa kering dan kepadatan populasi, namun secara nyata mempengaruhi laju pertumbuhan dan waktu penggandaan. Perlakuan pencahayaan 12, 18 dan 24 jam per hari menghasilkan laju pertumbuhan spesifik maksimum (0,345 sampai dengan 0,366 per hari) dan waktu penggandaan maksimum (1,89 sampai dengan 2,01 hari) yang tidak berbeda nyata, sedangkan perlakuan pencahayaan enam jam per hari menunjukkan laju pertumbuhan maksimum terendah (0.323 per hari) dan waktu penggandaan tertinggi (2,15 hari). Pada perlakuan pencahayaan 12 jam per hari, kandungan protein relatif lebih tinggi (39,73%) dari yang lain. Secara umum dapat disimpulkan bahwa  pencahayaan 12 jam per hari menghasilkan efisiensi produksi yang lebih baik daripada perlakuan lainnya.<br /><br />Key word: Spirulina sp., fotoperiode, kepadatan, biomassa, pertumbuhan, kandungan nutrisi.<br /></p>

E-beam irradiation affects the maximum specific growth rate ofBacillus cereus

2012 ◽  
Vol 48 (2) ◽  
pp. 382-386 ◽  
Author(s):  
Juan Aguirre ◽  
Mª Rosa Rodríguez ◽  
Rodrigo González ◽  
Gonzalo García de Fernando
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Beam Irradiation ◽  
Ofbacillus Cereus

scholarly journals Influence of the specific growth rate on formation of sterols in yeast Saccharomyces cerevisiae during fed-batch cultivation

2000 ◽  
Vol 18 (No. 3) ◽  
pp. 110-114
Author(s):  
J. Čermák ◽  
M. Rychtera ◽  
P. Nechvíle ◽  
J. Náhlík ◽  
K. Melzoch ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Control Strategy ◽  
Respiratory Quotient ◽  
Specific Growth ◽  
Carbon Dioxide Concentration ◽  
Sterol Fraction ◽  
Yeast Cells ◽  
Dry Biomass

Ergosterol is a major sterol in yeast cells. Intermediates of ergosterol biosynthesis or products of ergosterol biotransformation occur in cells too. Sterols mainly form components of cell membranes. Fluidity of membranes is affected by sterols. The amount of sterols in cells can be influenced above all by cultivation conditions and by the yeast genotype. Specific growth rate is an important factor which affects the amount of sterols present in yeast cells. We carried out a series of 24-hour cultivations to find out the impact of specific growth rate on sterol biosynthesis. Inflow of synthetic medium to the bioreactor was controlled by means of a profile of carbon dioxide concentration in the outlet gases. This profile was acquired by simulation according to a mathematical model of cultivation. Profile of carbon dioxide concentration corresponded to a precalculated profile of specific growth rate. Cultivation was divided into two phases with different growth rate values. A constant value of the specific growth rate was maintained in the 1st phase. The specific growth rate value decreased by controlling the inflow in the 2nd phase (beginning at 12th hour of cultivation). Other cultivations were carried out using so-called physiological control which consisted in determining the immediate physiological state (e.g., RQ) and the choice of control strategy according to the metabolic state. Selected control strategy ensures an immediate action (inflow of the medium). If the specific growth rate decreased in the 1st phase, the amount of total sterols in yeast dry biomass increased (to 2.7% in yeast dry biomass). But the purity of ergosterol decreased (amount of sterol contaminants increased up to 23.3% in the sterol fraction). If a constant value of respiratory quotient was maintained (at about 1.1), the amount of total sterols in yeast dry biomass and the purity of ergosterol were constant. If the value of respiratory quotient was changed in the growth and final phase of cultivation, the amount of total sterols in yeast dry biomass increased (to 2.83% in yeast dry biomass). However, the purity of ergosterol decreased (amount of sterol contaminants increased up to 21.2% in sterol fraction).

Maximum specific growth rate of anammox bacteria revisited

2017 ◽  
Vol 116 ◽  
pp. 296-303 ◽  
Author(s):  
Lei Zhang ◽  
Yuko Narita ◽  
Lin Gao ◽  
Muhammad Ali ◽  
Mamoru Oshiki ◽  
...  
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Anammox Bacteria
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