“Context, Please?” The Effects of Appearance- and Health-Frames and Media Context on Body-Related Outcomes

Promoting health-related behaviors such as healthy eating or doing sports are important to counteract the problem of obesity, which is on the rise. In this regard, initial studies suggest that appearance compared to health framing can lead to negative body-related outcomes in young women. This study aimed to extend these findings by investigating the role of the context. Furthermore, as previous studies focused on young women only, we considered a more diverse sample. This seems especially important as health campaigns focusing on healthy eating and sports should appeal to a more diverse population. This experimental study (N = 286) follows a 2 (appearance frame vs. health frame) × 2 (social media vs. magazine website) between-subjects design. Results revealed that exposure to appearance-focused framing led to a lower positive mood compared with exposure to health-focused framing. These effects were most prevalent in overweight and obese participants. Moreover, participants in the social media condition showed lower body satisfaction and lower positive mood compared with participants in the magazine website condition independent of the frame. No other interaction effects occured. Overall, health promoters should focus their campaigns on the health aspects of health-related behaviors and should consider promotion on social media platforms. Also, they should keep in mind that not only the framing, but also the context, might have effects on body-related outcomes.

Why Do You Trust News? The Event-Related Potential Evidence of Media Channel and News Type

Media is the principal source of public information, and people's trust in news has been a critical mechanism in social cohesion. In recent years, the vast growth of new media (e.g., internet news portals) has brought huge change to the way information is conveyed, cannibalizing much of the space of traditional media (e.g., traditional newspapers). This has led to renewed attention on media credibility. The study aims to explore the impact of media channel on trust in news and examine the role of news type. Twenty-six participants were asked to make trust–distrust decisions after reading a variety of news headlines from different media channels while undergoing electroencephalography (EEG) monitoring. The electrophysiological results showed that, for hard news (e.g., important news related to public life), the new media condition elicited smaller N100 and larger P200 amplitudes than the traditional media condition. However, for soft news (e.g., entertainment, and non-related to vital interest), there was no significant difference. The study suggests that the fitness of media channel and news type may influence the evaluation of news, impacting participants' affective arousal and attention allocation in the early stage and influencing trust in news. These results provide neurocognitive evidence of individuals' trust toward hard and soft news consumed via different media channels, yielding new insights into trust in media and contributing to media trust theory.

Design of flowing refractometer with cylindrical insert in the form a vertical pipeline section for control to clear media state

The article a the new design of flowing refractometer of differential type for media condition control both in laminar and turbulent flow regimes is considered. Given the peculiarities of the location of the flow and closed cuvette and the angles of incidence of laser radiation on their walls we have implemented a new method of measuring the refractive index n in new developed design of refractometer. This allowed us to make the influence of a number of errors related with unbalance voltage on the photoelectric converter, with an increase in the number of reflections between optical elements of laser radiation, with the presence of a transport link for the selection of flowing fluid and temperature fluctuations on the result of measuring the refractive index. The technique allowing to reduce the influence of change of the optical density of the flowing liquid on the measurement error of refractive index is proposed. The results of experimental investigations for different media are presented.

Muometric positioning system (μPS) with cosmic muons as a new underwater and underground positioning technique

Thus far, underwater and underground positioning techniques have been limited to those using classical waves (sound waves, electromagnetic waves or their combination). However, the positioning accuracy is strongly affected by the conditions of media they propagate (temperature, salinity, density, elastic constants, opacity, etc.). In this work, we developed a precise and entirely new three-dimensional positioning technique with cosmic muons. This muonic technique is totally unaffected by the media condition and can be universally implemented anywhere on the globe without a signal transmitter. Results of our laboratory-based experiments and simulations showed that, for example, plate-tectonics-driven seafloor motion and magma-driven seamount deformation can be detected with the μPS.

1207. Combining standard bacterial vaginosis treatment with cystine uptake inhibitors to block growth of Lactobacillus iners is a potential a target for shifting the cervicovaginal microbiota towards health-associated Lactobacillus crispatus-dominant communities

Background Cervicovaginal microbiota domination by Lactobacillus crispatus is associated with beneficial health outcomes, whereas L. iners dominance has more adverse associations. However bacterial vaginosis (BV) treatment with metronidazole (MTZ) typically leads to domination by L. iners rather than L. crispatus. L. iners differs from other lactobacilli by its inability to grow in MRS media. We hypothesized that exploring this growth difference would identify targets for selective L. iners inhibition. Methods Bacteria were grown anaerobically. Nutrient uptake and metabolism were assessed using UPLC-MS/MS and isotopically labeled substrates. Bacterial genome annotation employed Prodigal, Roary, and EggNOG. Competition experiments with mock mixed communities were analyzed by 16S rRNA gene sequencing. We confirmed result generalizability using a diverse collection of South African and North American strains and genomes. Results Supplementing MRS broth with L-cysteine (Cys) or L-cystine permitted robust L. iners growth, while L. crispatus grew without Cys supplementation. Despite their different growth requirements, neither species could synthesize Cys via canonical pathways. Adding the cystine uptake inhibitors S-methyl-L-cysteine (SMC, Fig 1) or seleno-DL-cystine (SDLC) blocked growth of L. iners but not other lactobacilli, suggesting L. iners lacks mechanisms other lactobacilli use to exploit complex exogenous Cys sources. Notably, cydABCD, an operon with Cys/glutathione transport and redox homeostasis activities, is absent from L. iners but present in non-iners Lactobacillus species. Consistent with possible roles for cydABCD in explaining the observed phenotypes, (1) L. iners failed to take up exogenous glutathione and (2) supplementing MRS with reducing agents permitted L. iners growth, which could be blocked by SMC or SDLC. In growth competitions testing L. iners and L. crispatus within mock BV-like communities, SMC plus MTZ outperformed MTZ alone in promoting L. crispatus dominance (Figs 2&3). Figure 1: S-methyl-L-cysteine (SMC) selectively blocks growth of L. iners but not other cervicovaginal Lactobacillus species in cysteine-supplemented MRS broth. Growth was measured by optical density and inhibition calculated relative to Cys-supplemented no-inhibitor control during exponential growth. Values displayed are median (+/- maximum/minimum) for 3 replicates from a single experiment. In all panels, representative data are shown from 1 of >=2 independent experiments for each bacterial strain and media condition. Results are representative of multiple strains for L. iners (n = 16), L. crispatus (n = 7), and L. jensenii (n = 2). Figure 2: Relative abundance of L. crispatus, L. iners, or various BV-associated bacteria in mock bacterial communities grown in rich, non-selective media with or without metronidazole (MTZ) and/or SMC. Relative abundance was determined by bacterial 16S rRNA gene sequencing. Data are shown for three representative mock communities with 5 replicates per media condition. Figure 3: Ratio of L. crispatus to other species in the mock bacterial communities depicted in Figure 2. Statistical significance determined via 1-way ANOVA of log10-transformed ratios with post-hoc Tukey test; selected pairwise comparisons are shown (***, p < 0.001). Conclusion L. iners has unique requirements for exogenous cysteine/cystine or a reduced environment for growth. Targeting cystine uptake to inhibit L. iners is a potential strategy for shifting cervicovaginal microbiota towards L. crispatus-dominant communities. Disclosures Douglas S. Kwon, MD, PhD, Day Zero Diagnostics (Consultant, Shareholder, Other Financial or Material Support, co-founder)

Two-Phase Thermal Metamaterial

The capability of thermal metamaterials is required from single function to multifunction under different external heat conditions. The methods to develop thermal materials by simple structural transformations have been explored. While, the components of traditional thermal metamaterial are mainly set as solid materials, which is difficult to change the composition of materials, such as recombing and fixing the spatial position of material, because of material rigidity. Therefore, the potential of thermal materials is limited. Liquid has fluidity in spatial structure, for which the efficient combination of solid-liquid materials provides an avenue for dynamically modeling thermal field. Herein, we propose the concept of two-phase thermal metamaterial, which is switchable by microscale elements. On one side, we develop a switchable thermal meta-unit manipulated by micro-element under the gradient field and explore the process of heat transfer by focusing on radiation and conduction under translucent media condition. Otherwise, we propose a method to achieve a non-reciprocal heat transfer system by the design of two-phase media. The propose of two-phase thermal metamaterials set a general background for a variety of applications for complex conditions.

The “Jim Cantore Effect?” A Preliminary Test of Individual Difference-Induced Media Effects Upon Hurricane Evacuation Decisions

We conducted this study to test for an interaction of media effects and individual differences that could affect hurricane evacuation decision-making. Participants responded in an online, Qualtrics-hosted survey to many demographic and individual difference questionnaires assessing decisions one would make in a hurricane evacuation. Participants were randomly assigned to one of two video conditions (reporter emotionality: high or low) and asked a number of questions about the field-reporting meteorologist they viewed. We predicted an interaction between intuitive thinking (which is faster and more emotional) and emotional reporting such that highly intuitive thinkers would be more likely to evacuate when presented with more emotional hurricane field reporting. This hypothesis was rejected. However, there was a main effect of intuition, whereby highly intuitive people expressed more fear for weather (but not hurricanes). Furthermore, there was a main effect of media condition: People exposed to highly emotional field reporting in general were more likely to evacuate. These results are discussed in light of meteorological communication standards and principles.

Production of the secondary metabolite catechin by in vitro cultures of Camellia sinensis L

BackgroundCatechin is one of the secondary metabolites in Camellia sinensis L. that is alternatively produced through in vitro cultures. The in vitro culture product is possibly improved by optimizing the culture medium with the addition of growth regulators and precursors. The purpose of this study was to confirm the success of the secondary catechin metabolite production through the in vitro culture of C. sinensis L in a relatively short time.MethodsThe secondary catechin metabolite product is obtained in about 40 days. The study was conducted by (1) leaf cutting for inoculation in Murashige and Skoog media with 1 μg/mL of 2,4-dichlorophenoxyacetic acid growth regulator; (2) the inoculation of callus multiplication on the same medium as a partially modified inoculation media condition with the addition of 1 μg/mL of 6-benzylaminopurine (BAP) and 2 μg/mL of 2,4-dichlorophenoxyacetic acid at concentration; (3) callus multiplication developed on a new medium containing phenylalanine precursors (300 μg/mL); (4) testing growth by harvesting the callus and weighing the wet weight of its biomass and (5) identification of the callus qualitatively and quantitatively by using high-performance liquid chromatography (HPLC).ResultsThe level of secondary catechin metabolite produced was 2.54 μg/mL and 12.13 μg/mL in solid and suspension media, respectively.ConclusionsIt is concluded that the method is effective and efficient in producing catechin product from C. sinensis L.

Impact of cortical mastoidectomy on tympanoplasty for recurrent suppurative otitis media: a prospective study

<p class="abstract"><strong>Background:</strong> Chronic suppurative otitis media condition is characterized by an ear discharge and a perforation in the tympanic membrane. Tympanoplasty and tympanoplasty with mastoidectomy are the two common surgeries performed to manage this condition.</p><p class="abstract"><strong>Methods:</strong> 50 patients were divided into two groups of 25 each. On Group I, tympanoplasty alone was done and on Group II tympanoplasty along with mastoidectomy was performed and outcome was noted. </p><p class="abstract"><strong>Results:</strong> The most common age group to be affected was 21-30 years. Discharge of 1-3 years was observed in 32% in Group I and 36% in Group II. 84% in Group I and 72% in Group II had mild hearing loss, and in both the groups, moderate perforations were more common. 84% of the patients in Group I and 88% in group II showed acceptance to the surgeries and the hearing gain in both the groups was around 10.</p><p class="abstract"><strong>Conclusions:</strong> This study shows that tympanoplasty with mastoidectomy has no added advantage over tympanoplasty alone in the patients with chronic suppurative otitis media.</p>