scholarly journals Accurate localization of linear probe electrode arrays across multiple brains

eNeuro ◽  
2021 ◽  
pp. ENEURO.0241-21.2021
Author(s):  
Liu D Liu ◽  
Susu Chen ◽  
Han Hou ◽  
Steven J West ◽  
Mayo Faulkner ◽  
...  
Author(s):  
Liu D Liu ◽  
Susu Chen ◽  
Michael N Economo ◽  
Nuo Li ◽  
Karel Svoboda

AbstractRecently developed silicon probes have large numbers of recording electrodes on long linear shanks. Specifically, Neuropixels probes have 960 recording electrodes distributed over 9.6 mm shanks. Because of their length, Neuropixels probe recordings in rodents naturally span multiple brain areas. Typical studies collate recordings across several recording sessions and animals. Neurons recorded in different sessions and animals have to be aligned to each other and to a standardized brain coordinate system. Here we report a workflow for accurate localization of individual electrodes in standardized coordinates and aligned across individual brains. This workflow relies on imaging brains with fluorescent probe tracks and warping 3-dimensional image stacks to standardized brain atlases. Electrophysiological features are then used to anchor particular electrodes along the reconstructed tracks to specific locations in the brain atlas and therefore to specific brain structures. We performed ground-truth experiments, in which motor cortex outputs are labelled with ChR2 and a fluorescence protein. Recording from brain regions targeted by these outputs reveals better than 100 μm accuracy for electrode localization.


2021 ◽  
Author(s):  
James Sun ◽  
Katrina Barth ◽  
Shaoyu Qiao ◽  
Chia-Han Chiang ◽  
Charles Wang ◽  
...  

AbstractOne-third of epilepsy patients suffer from medication-resistant seizures. While surgery to remove epileptogenic tissue helps some patients, 30–70% of patients continue to experience seizures following resection. Surgical outcomes may be improved with more accurate localization of epileptogenic tissue. We have previously developed novel thin-film, subdural electrode arrays with hundreds of microelectrodes over a 100–1,000 mm2 area to enable high-resolution mapping of neural activity. Here we used these high-density arrays to study microscale properties of human epileptiform activity. We performed intraoperative micro-electrocorticographic recordings within epileptic cortex (the site of seizure onset and early spread) in nine patients with epilepsy. In two of these patients, we obtained recordings from cortical areas distal to the epileptic cortex. Additionally, we recorded from two non-epileptic patients with movement disorders undergoing deep brain stimulator implantation as non-epileptic tissue controls. A board-certified epileptologist identified microseizures, which resembled electrographic seizures normally observed with clinical macroelectrodes. Epileptic cortex exhibited a significantly higher microseizure rate (2.01 events/min) than non-epileptic cortex (0.01 events/min; permutation test, P=0.0068). Using spatial averaging to simulate recordings from larger electrode contacts, we found that the number of detected microseizures decreased rapidly with increasing contact diameter and decreasing contact density. In cases in which microseizures were spatially distributed across multiple channels, the approximate onset region was identified. Our results suggest that micro-electrocorticographic electrode arrays with a high density of contacts and large coverage are essential for capturing microseizures in epilepsy patients and may be beneficial for localizing epileptogenic tissue to plan surgery or target brain stimulation.


Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.


2019 ◽  
Author(s):  
Nasir Saeed ◽  
Mohamed-Slim Alouini ◽  
Tareq Y. Al-Naffouri

<div>Localization is a fundamental task for optical internet</div><div>of underwater things (O-IoUT) to enable various applications</div><div>such as data tagging, routing, navigation, and maintaining link connectivity. The accuracy of the localization techniques for OIoUT greatly relies on the location of the anchors. Therefore, recently localization techniques for O-IoUT which optimize the anchor’s location are proposed. However, optimization of anchors location for all the smart objects in the network is not a useful solution. Indeed, in a network of densely populated smart objects, the data collected by some sensors are more valuable than the data collected from other sensors. Therefore, in this paper, we propose a three-dimensional accurate localization technique by optimizing the anchor’s location for a set of smart objects. Spectral graph partitioning is used to select the set of valuable</div><div>sensors.</div>


2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Eslam Mounier ◽  
Bassem Abdullah ◽  
Hani Mahdi ◽  
Seif Eldawlatly

AbstractThe Lateral Geniculate Nucleus (LGN) represents one of the major processing sites along the visual pathway. Despite its crucial role in processing visual information and its utility as one target for recently developed visual prostheses, it is much less studied compared to the retina and the visual cortex. In this paper, we introduce a deep learning encoder to predict LGN neuronal firing in response to different visual stimulation patterns. The encoder comprises a deep Convolutional Neural Network (CNN) that incorporates visual stimulus spatiotemporal representation in addition to LGN neuronal firing history to predict the response of LGN neurons. Extracellular activity was recorded in vivo using multi-electrode arrays from single units in the LGN in 12 anesthetized rats with a total neuronal population of 150 units. Neural activity was recorded in response to single-pixel, checkerboard and geometrical shapes visual stimulation patterns. Extracted firing rates and the corresponding stimulation patterns were used to train the model. The performance of the model was assessed using different testing data sets and different firing rate windows. An overall mean correlation coefficient between the actual and the predicted firing rates of 0.57 and 0.7 was achieved for the 10 ms and the 50 ms firing rate windows, respectively. Results demonstrate that the model is robust to variability in the spatiotemporal properties of the recorded neurons outperforming other examined models including the state-of-the-art Generalized Linear Model (GLM). The results indicate the potential of deep convolutional neural networks as viable models of LGN firing.


2021 ◽  
pp. 2004033
Author(s):  
Estelle A. Cuttaz ◽  
Christopher A. R. Chapman ◽  
Omaer Syed ◽  
Josef A. Goding ◽  
Rylie A. Green

Author(s):  
Stephan Schlupkothen ◽  
Gerd Ascheid

Abstract The localization of multiple wireless agents via, for example, distance and/or bearing measurements is challenging, particularly if relying on beacon-to-agent measurements alone is insufficient to guarantee accurate localization. In these cases, agent-to-agent measurements also need to be considered to improve the localization quality. In the context of particle filtering, the computational complexity of tracking many wireless agents is high when relying on conventional schemes. This is because in such schemes, all agents’ states are estimated simultaneously using a single filter. To overcome this problem, the concept of multiple particle filtering (MPF), in which an individual filter is used for each agent, has been proposed in the literature. However, due to the necessity of considering agent-to-agent measurements, additional effort is required to derive information on each individual filter from the available likelihoods. This is necessary because the distance and bearing measurements naturally depend on the states of two agents, which, in MPF, are estimated by two separate filters. Because the required likelihood cannot be analytically derived in general, an approximation is needed. To this end, this work extends current state-of-the-art likelihood approximation techniques based on Gaussian approximation under the assumption that the number of agents to be tracked is fixed and known. Moreover, a novel likelihood approximation method is proposed that enables efficient and accurate tracking. The simulations show that the proposed method achieves up to 22% higher accuracy with the same computational complexity as that of existing methods. Thus, efficient and accurate tracking of wireless agents is achieved.


Author(s):  
Rosen Ivanov

The majority of services that deliver personalized content in smart buildings require accurate localization of their clients. This article presents an analysis of the localization accuracy using Bluetooth Low Energy (BLE) beacons. The aim is to present an approach to create accurate Indoor Positioning Systems (IPS) using algorithms that can be implemented in real time on platforms with low computing power. Parameters on which the localization accuracy mostly depends are analyzed: localization algorithm, beacons’ density, deployment strategy, and noise in the BLE channels. An adaptive algorithm for pre-processing the signals from the beacons is proposed, which aims to reduce noise in beacon’s data and to capture visitor’s dynamics. The accuracy of five range-based localization algorithms in different use case scenarios is analyzed. Three of these algorithms are specially designed to be less sensitive to noise in radio channels and require little computing power. Experiments conducted in a simulated and real environment show that using proposed algorithms the localization accuracy less than 1 m can be obtained.


2021 ◽  
Vol 13 (4) ◽  
pp. 544
Author(s):  
Guohao Zhang ◽  
Bing Xu ◽  
Hoi-Fung Ng ◽  
Li-Ta Hsu

Accurate localization of road agents (GNSS receivers) is the basis of intelligent transportation systems, which is still difficult to achieve for GNSS positioning in urban areas due to the signal interferences from buildings. Various collaborative positioning techniques were recently developed to improve the positioning performance by the aid from neighboring agents. However, it is still challenging to study their performances comprehensively. The GNSS measurement error behavior is complicated in urban areas and unable to be represented by naive models. On the other hand, real experiments requiring numbers of devices are difficult to conduct, especially for a large-scale test. Therefore, a GNSS realistic urban measurement simulator is developed to provide measurements for collaborative positioning studies. The proposed simulator employs a ray-tracing technique searching for all possible interferences in the urban area. Then, it categorizes them into direct, reflected, diffracted, and multipath signal to simulate the pseudorange, C/N0, and Doppler shift measurements correspondingly. The performance of the proposed simulator is validated through real experimental comparisons with different scenarios based on commercial-grade receivers. The proposed simulator is also applied with different positioning algorithms, which verifies it is sophisticated enough for the collaborative positioning studies in the urban area.


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