scholarly journals Antihyperglycemic Activity of Aqueous Extract of Insulin Leaves (Tithonia diversifolia) on Hyperglycemic Rats (Rattus norvegicus)

2018 ◽  
Vol 10 (3) ◽  
pp. 636-641
Author(s):  
Anisa Rachma Sari ◽  
Tyas Rini Saraswati ◽  
Enny Yusuf Wachidah Yuniwarti
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Rattus Norvegicus ◽  
Tithonia Diversifolia ◽  
Antihyperglycemic Activity ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Decrease Blood Glucose

Insulin leave (Tithonia diversifolia (Hemsl.) A. Gray) empirically used by people as antihyperglycemic drugs. The study was aimed to evaluate antihyperglycemic activity as well as to determine the most optimum dose of T. diversifolia to reduce blood glucose levels. Hyperglycemic condition was induced to male Rattus norvegicus rats by intraperitoneal injection of alloxan at a dose of 150 mg/kg bw (body weight). In this study, completely randomized design was performed with three treatment groups and five times repetition. Group P1 received glibenclamide 10 mg/kg bw, P2 received aqueous extract of T. diversifolia at a dose of 150 mg/kg bw, P3 received aqueous extract of T. diversifolia at a dose of 300 mg/kg bw. Administration of both glibenclamide and aqueous extract of T. diversifolia was conducted orally for 28 days. Data were analyzed using Anova and Duncan’s test with 95% confidence level (α = 0.05). The results showed that the mean percentage of decrease in blood glucose levels, drink intake and body weight of all treatment groups were not significantly different (p>0.05). Data of feed intake showed that P1 was significantly different from P2 (p<0.05), but P2 was not significantly different from P3 (p>0.05). Based on the result of this research, it was found that the low doses of aqueous extract of T. diversifolia has the same ability to decrease blood glucose level compared to glibenclamide. Futhermore, this study provide some information that can be used as preclinical analysis to determine effective doses of aqueous extract of T. diversifolia to decrease blood glucose levels.  

scholarly journals Test Antihyperglycemia Ethanol Leaf Extract Kembang Bulan ( Tithonia diversifolia) in Mice ( Mus musculus ) The Induced Diabetes With alloxan

2016 ◽  
Vol 1 (2) ◽  
pp. 36
Author(s):  
Ronald Pasaribu ◽  
Salomo Hutahaean ◽  
Syafruddin Ilyas
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Ethanol Extract ◽  
Giant Cells ◽  
Tithonia Diversifolia ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Weight Effect

A research was conducted to invenstigate antihyperglycemic activity ethanol extract of leaf kembang bulan (Tithonia diversifolia) in mice with alloxan induced diabetes. The experiment used completely randomized design, 6 treatment groups with 5 replicates each. KP mice, P1, P2, P3 and P4 fasted for 16 hours and then injected intraperitoneally alloxan 5.04 mg. On day 4 post induction, KP, P1, P2, P3 and P4 positive diabetes. P1, P2 and P3 were treated 14; 42 and 70 mg/Kg of ethanol extract of kembang bulan while P4 were 0.9 mg/Kg glibenclamide for 12 days. The results showed that all treatment doses did not significantly affect body weight of diabetic mice (P>0.05). P1 obtained with a dose of 14 mg/Kg body weight is able to decrease blood glucose levels (P<0,05). The ethanol extract at a dose of 14 mg/Kg body weight effect on decreasing the number of giant cells of the spleen. It can be concluded ethanol extract of kembang bulan can lower blood glucose levels  and effect  the  microstructure spleen of mice  with alloxan induced diabetes. Keywords : Alloxan , Ethanol Extract , Tithonia diversifolia , Blood Glucose Levels.

scholarly journals Pengaruh Ekstrak Rebusan Daun Tithonia diversifolia (Hemsl.) A. Gray Terhadap Kadar Glukosa Darah

2019 ◽  
Vol 7 (1) ◽  
pp. 41
Author(s):  
Rinawati Rinawati ◽  
E Suharyanto ◽  
Nastiti Wijayanti
Keyword(s):  
Blood Glucose ◽  
Young Leaf ◽  
Tithonia Diversifolia ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Randomized Design ◽  
Adult Leaf ◽  
Reduce Blood Glucose ◽  
Completely Randomized Design

Tithonia diversifolia is a plant that has the potential to reduce blood glucose levels that is crucial in Diabetes Mellitus therapy. This study aims to determine the active compounds contained in the extract of T. diversifolia leaves decoction, the effect of decoction extract on the reduction of blood glucose, and the most effective decoction extract to reduce blood glucose levels as well. The study used a Completely Randomized Design (CRD) with the research treatment groups, namely: Kn = normal control (normal mice and not given leaves decoction extract), Ka = STZ control (DM mice, which are normal mice induced by 65mg/Kg of STZ), Kp = treatment control (normal mice given leaf decoction extract) , P1 = DM + mice were given young leaf decoction extract, P2 = DM + mice were given a mixture of young leaf and adult leaf decoction extract, P3 = DM + mice were given adult leaf decoction extract. The leaves used for decoction were 1-6 leaves from the shoots. The analysis of T. diversifolia leaves decoction compounds is using the visible spectrophotometers for tannins, phenols and flavonoids and GC-MS for terpenoid analysis. The result found that Tithonia diversifolia leaves decoction extracts contain tannins, flavonoids and phenols, while terpenoids are not detected. The leaves decoction extracts of T. diversifolia influenced the decreases in blood glucose levels of DM mice, especially the decoction extract of adult leaves which reduced blood glucose levels to 71.16%.

scholarly journals Antidiabetic activity of curry leaves Murraya koenigii on glucose level, kidney and islets of langerhans in streptozotocin induced diabetes in rats

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Imad M. Al-Ani ◽  
Rahajoe I. Santosa ◽  
Muhammad H. Yankuzo
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Diabetic Control ◽  
Diabetic Rats ◽  
Murraya Koenigii ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Curry Leaves ◽  
Aqueous Leaf Extract

Introduction: This study examined the antihyperglycemic effect of curry leaves, Murraya koenigii “MK” aqueous extract, and to examine its possible protective effects on the Islets of Langerhans and kidneys in streptozotocin (STZ) induced diabetic rats.  Methods: Thirty healthy adult male Sprague Dawley rats were randomized into five groups (n=6); normal control, normal treated with “MK” control, diabetic control (non-treated with “MK”), diabetic treated with 200mg/kg MK aqueous leaf extract and diabetic treated with 400mg/kg MK aqueous leaf extract. Blood glucose levels and body weight were monitored. The animals were sacrificed on the 30th day; the kidney and pancreatic tissues were processed for histological studies. Results: The diabetic control group significantly (p<0.001) showed considerable loss of body weight and increase in blood glucose levels and degeneration of the glomeruli and renal convoluted tubules and atrophied islets with disintegration of β-cells. Treatment of diabetic rats with aqueous extract showed significant (p<0.001) improvement in blood glucose levels and body weight gain.  The MK extract also caused an improvement in tissue injury induced by STZ injection in the kidney and endocrine pancreas.  Conclusions: These findings highlighted the beneficial effects of MK aqueous extract against cellular oxidative damage in STZ-induced diabetic rats.

Potent Antihyperglycemic Effects of an Endemic Plant from Morocco (Matthiola maroccana Coss.) in Normal and Streptozotocin-Induced Diabetic Rats

2020 ◽  
Vol 20 ◽  
Author(s):  
Ayoub Amssayef ◽  
Nadia Lahrach ◽  
Mohamed Eddouks
Keyword(s):  
Antioxidant Activity ◽  
Body Weight ◽  
Blood Glucose ◽  
Aqueous Extract ◽  
Histopathological Examination ◽  
Diabetic Rats ◽  
Oral Glucose ◽  
Endemic Plant ◽  
Glucose Levels ◽  
Blood Glucose Levels

Aims: The study aims to evaluate the antidiabetic effect of Matthiola maroccana (Coss.). Background: Matthiola maroccana (Coss.) belongs to Brassicaceae family is an endemic plant from Morocco. Objective: The objective of the study was to evaluate the effect of its aqueous extract on blood glucose levels in normal and diabetic rats. Method: Single dose (6 hours) and daily oral administration for seven days of the Aerial Part Aqueous Extract (A.P.A.E) of Matthiola maroccana (Coss.) (M. maroccana) at a dose of 20 mg/kg body weight on blood glucose levels both in normal and streptozotocin(STZ)-induced diabetic rats. Furthermore, body weight, oral glucose tolerance test, liver histopathological examination, phytochemical screening, and in vitro antioxidant activity of A.P.A.E were performed in this study. Results: The results showed that M. maroccana A.P.A.E exerts potent hypoglycemic and antihyperglycemic effects in normal and STZ-induced diabetic rats (p<0.0001). Also, it was able to restore body weight in diabetic rats (p<0.05). Furthermore, the aqueous extract has been shown to regenerate hepatic tissues in diabetic rats. In addition, A.P.A.E revealed the presence of several phytochemical constituents (polyphenols, flavonoids, tannins, saponins, alkaloids, sterols and terpenoids), and possessed an antioxidant activity. Conclusion: In conclusion, our findings showed that A.P.A.E of M. maroccana (A.P.A.E MM) possesses a significant antihyperglycemic and hypoglycemic activity.

scholarly journals Hibiscus noldea (Malvaceae) Aqueous Extract Prevents Insulin Resistance and Protects Pancreatic Islets From Dexamethasone Damages in Rat.

2017 ◽  
Vol 9 (2) ◽  
Author(s):  
Ngueguim Tsofack Florence ◽  
Djientcheu Tientcheu Jean Philippe ◽  
Donfack Jean Hubert ◽  
Gounoue Kamkumo Raceline ◽  
Dzeufiet Djomeni Paul Desire ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Body Weight ◽  
Blood Glucose ◽  
Lipid Profile ◽  
Pancreatic Islets ◽  
Aqueous Extract ◽  
Hepatic Glycogen ◽  
Dependent Manner ◽  
Glucose Levels ◽  
Blood Glucose Levels

<p><em>Hibiscus noldea</em> leaves-stems aqueous extract is used in Cameroonian traditional medecine to manage diabetes. To investigate the preventive effect of <em>Hibiscus noldea</em> aqueous extract on dexamethasone-induced insulin resistance, the animals received one of the following treatments: distilled water (10 mL/kg), metformine (200 mg/kg), or <em>H. noldea</em> (100 or 200 mg/kg) concomitantly with dexamethasone (0.5 mg/kg, ip) for ten days. Body weight was evaluated daily and blood glucose levels were measured. At the end of experiment, insulin sensitivity test was performed and lipid profile, transaminases Aspartate amino transferase, Alanin amino transferase, malondialdehyde, superoxide dismutase, catalase, and reduced glutathione were evaluated. Histological analysis of the liver was investigated to estimate glycogen content using Periodic Acid Schiff coloration and histomorphometry of pancreatic islets area was performed.</p><p>The administration of dexamethasone during ten days induced body weight loss, hyperglycaemia, insulinresistance, an imbalance in lipid profile, an increase in transaminases and oxidative stress. Dexamethasone treatment also induced an increase in the pancreatic islets area and depletion in the levels of hepatic glycogen. Concomitant administration of dexamethasone and the aqueous plant extract prevented the rise in blood glucose levels, reduced insulinresistance, improved lipid profile and oxidative status. The aqueous extract of <em>H. noldea</em> prevented the use of glycogen storage and the increase in pancreatic islet area in dose dependent manner.</p><p>Conclusion: The stem leaves aqueous extract from <em>Hibiscus noldea</em> have the ability to reduce insulinresistance via its antihyperglycaemic, hypolipidemic and antioxidant activities. These results justify the use of this extract in the management of diabetic state.</p>

scholarly journals Potency of Swallow Nest Extract (Collocalia fuciphago) as Pancreaprotector Through Oxidative Mechanism in Rats (Rattus norvegicus) Induced by Streptozotocin

2018 ◽  
Vol 14 (1) ◽  
pp. 15
Author(s):  
Agnes Frethernety ◽  
Satriyandi M ◽  
Francisca Diana Alexandra
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Fasting Blood Glucose ◽  
Water Extract ◽  
Clinical Syndrome ◽  
Control Group ◽  
P Value ◽  
Treatment Groups ◽  
Glucose Levels ◽  
Blood Glucose Levels

Abstract: Diabetes mellitus is a clinical syndrome with characteristics of hyperglycemia. Hyperglycemia can lead to the formation of excessive free radical compounds that trigger oxidative stress, especially on pancreatic β cells. Swallow nest contains amino acids which are insulin-forming compounds and glutathione that can act as a major reducing agent and antioxidant defense. The purpose of this study is to analyze the effect of swallow’s nest water extract as a pancreaprotector through oxidative mechanism in rats (Rattus norvegicus) induced by streptozotocin. The rats induced by streptozotocin at a dose of 40 mg/kg BW and then randomly divided into 4 groups. Control groups were given aquades and 3 treatment groups, respectively were administered swallow’s nest water extract at doses of 1, 10, 100 mg/kg BW for 21 days. Fasting blood glucose levels were measured on 7, 14 and 21 day. MDA measured after  the animal is determined  at day 28th.Level of  fasting blood glucose and MDA were analyzed by statistic using Kruskal Wallis test followed by multiple comparisons Mann Whitney test. There was a significant decrease in fasting blood glucose level with p = 0.04 in all treatment groups compared the control group. The administration of swallow nest extract significantly decreased MDA pancreas with p value = 0.000 and dose 100 mg/kgBW extract of swallow’s nest significantly decrease pancreatic MDA level. The administration of swallow’s nest water extract shows decrease of fasting blood sugar and has a protective effect on the rats’ pancreas  through oxidative mechanism. Keywords: Swallow's nest extract, MDA

scholarly journals Hiperglikemia Tidak Menyebabkan Perubahan Bobot Badan dan Jumlah Neuron Mienterik Nitrergik Usus Halus Tikus Wistar (Rattus norvegicus)

2019 ◽  
Vol 36 (2) ◽  
pp. 258
Author(s):  
Amelia Hana ◽  
Pudji Astuti ◽  
Yuda Heru Fibrianto ◽  
Sarmin Sarmin ◽  
Claude Mona Airin ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Rattus Norvegicus ◽  
The Body ◽  
Enteric Neurons ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Group I ◽  
Wistar Strain ◽  
Small Intestines

Digestive dysfunction is a common symptom of diabetes. Diabetes can lead to the loss of enteric neurons as well as nerve dysfunction and gastrointestinal complications. There has been no complete report of mienteric nitrergic of small intestines on hyperglycaemia condition, so the purpose of this study was to assess changes of nitrergic neural profile of small intestines on hyperglycemic Wistar strain rats (Rattus norvegicus). Twelve rats were adapted for 3 days, then they were randomly divided into 3 groups namely group I (K-1, as control), group II (K-2), and group III (K-3). Before treatment all rats were fasted for 12 hours, rats K-1 were induced with 1.0 ml of buffer citrate single peritoneal dose; rats K-2 were induced with 40 mg streptozotocin (STZ)/ kg body weight of single peritoneal dose; and rats K-3 were induced with 45 mg STZ /kg body weight of single peritoneal dose. On the 1st and 5th days after STZ induction, blood was collected through the coccigea vein to measure blood glucose levels and weigh the weight. On the day 5th the rats were uthanated with a high dose of ketamine, dissected and taken small intestines (duodenum, jejunum, and ileum) for staining with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d). Morphometric changes of nitrergic neurons of the small intestine segments were observed. The results showed that on the 5th day increased blood glucose levels of the K-2 and K-3 groups were significantly higher than K-1 (P <0.01). The weight of three groups did not show any significant improvement difference (P> 0.05). The number of neurons in the duodenum, jejunum and ileum of K-2 group increased higher than that of K-1 and K-3 groups. These results indicate that in hyperglycaemic states, the number of nitrergic neurons increases (K-2 group), and there is a significant decrease in the number of neurons (group K-3) compared with K-1 and K-2 groups (P <0.01). The results of this study concluded, that the STZ induction treatment causes hyperglycemia. Hyperglycemia does not affect to the body weight and the number of  nitrergic neurons of small intestines in Wistar strain rats (Rattus norvegicus).

scholarly journals Uji efek ekstrak daun sirih hutan (Piper aduncum L.) terhadap kadar gula darah pada tikus wistar (Rattus novergicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Silvia R.H. Sitinjak ◽  
Jane Wuisan ◽  
Christi Mambo
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Betel Leaf ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Decrease Blood Glucose ◽  
Piper Aduncum

Abstract: Forest betel leaf (Piper aduncum L.) has been known bypublicto have efficacy in wound healing, stop vomiting, reducing nausea, aiding digestion, as an antiseptic, as well as killing bacteria, fungi and viruses. Dewi et al. found that 50 mg/kgBW and 100 mg/kgBW red betel leaf extract caused a decrease blood glucose levels in Wistar rats (Rattus norvegicus) induced by alloxan. This study was aimed to identify the effect of forest betel leaf extract on blood glucose levels on Wistar rats induced by alloxan. Subjects were Wistar rats with total of 15 samples divided into five groups: a negative control group and four hyperglycemic groups induced by 130 mg/kgBW of alloxan. Forest betel leaf extract was administered with dose of 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW on each groups of hyperglycemic rats, and group0.4 IU/100grBW of novomix injection was administered on the positive control group. Blood glucose levels were measured in the 0, 30, 60, 90, and 120 minutes on day one, two, and three. The results showed that 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW of forest betel leaf extract had a tendency to decrease blood glucose levels of Wistar rats induced by alloxan.Keywords: piper aduncum L., forest betel leaf, blood glucose levels, alloxan Abstrak: Daun sirih hutan (Piper aduncum L.) telah dikenal oleh masyarakat dan mempunyai khasiat dalam penyembuhan luka, menghentikan muntah, mengurangi mual, melancarkan pencernaan, sebagai antiseptik, membunuh bakteri dan jamur serta virus. Menurut penelitian Dewi dkk, pemberian ekstrak daun sirih merah dengan dosis 50 mg/kgBB dan 100 mg/kgBB menyebabkan penurunan kadar gula darah pada tikus Wistar (Rattus norvegicus) yang diinduksi dengan aloksan. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun sirih hutan terhadap kadar gula darah pada tikus Wistar yang diinduksi dengan aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok terdiri atas kelompok kontrol negatif dan 4 kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB tikus yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB tikus, dan 100 mg/kgBB tikus, dan yang terakhir kelompok kontrol positif diberi suntikan novomix 0,4 IU/100grBB tikus. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok pada hari pertama, kedua, dan ketiga pada menit ke-0, 30, 60, 90, dan 120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB dan 100 mg/kgBB tikus memiliki kecenderungan untuk menurunkan kadar gula darah tikus Wistar yang diinduksi oleh aloksan. Kata kunci: piper aduncumL., daun sirih hutan, kadar gula darah, aloksan

Evaluation of Glucose and Lipid Lowering Activity of Arganimide A in Normal and Streptozotocin-Induced Diabetic Rats

2019 ◽  
Vol 19 (4) ◽  
pp. 503-510 ◽  
Author(s):  
Mohamed Eddouks ◽  
Farid Khallouki ◽  
Robert W. Owen ◽  
Morad Hebi ◽  
Remy Burcelin
Keyword(s):  
Body Weight ◽  
Blood Glucose ◽  
Oral Administration ◽  
Lipid Profile ◽  
Plasma Cholesterol ◽  
Diabetic Rats ◽  
Lipid Lowering ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Lowering Activity

Aims: Arganimide A (4,4-dihydroxy-3,3-imino-di-benzoic acid) is a compound belonging to a family of aminophenolics found in fruit of Argania spinosa. The purpose of this study was to investigate the glucose and lipid lowering activity of Arganimide A (ARG A). Methods: The effect of a single dose and daily oral administration of Arganimide A (ARG A) on blood glucose levels and plasma lipid profile was tested in normal and streptozotocin (STZ) diabetic rats at a dose of 2 mg/kg body weight. Results: Single oral administration of ARG A reduced blood glucose levels from 26.50±0.61 mmol/L to 14.27±0.73 mmol/L (p<0.0001) six hours after administration in STZ diabetic rats. Furthermore, blood glucose levels were decreased from 5.35±0.30 mmol/L to 3.57±0.17 mmol/L (p<0.0001) and from 26.50±0.61 mmol/L to 3.67±0.29 mmol/L (p<0.0001) in normal and STZ diabetic rats, respectively, after seven days of treatment. Moreover, no significant changes in body weight in normal and STZ rats were shown. According to the lipid profile, the plasma triglycerides levels were decreased significantly in diabetic rats after seven days of ARG treatment (p<0.05). Moreover, seven days of ARG A treatment decreased significantly the plasma cholesterol concentrations (p<0.001). Conclusion: ARG A possesses glucose and lipid-lowering activity in diabetic rats and this natural compound may be beneficial in the treatment of diabetes.

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