STUDIES ON A SEROLOGICAL PREGNANCY TEST: III

1963 ◽  
Vol 42 (3) ◽  
pp. 395-402 ◽  
Author(s):  
S. Kaivola ◽  
U. Kiistala ◽  
E. Axelson

ABSTRACT Improvement has been made in a serological haemagglutination inhibition method for the qualitative and quantitative determination of chorionic gonadotrophin (HCG) in urine, using a highly purified, immunologically homogenous HCG in the preparation of antisera and in the coating of blood cells. Furthermore agar diffusion tests have been carried out with a view to clarifying the immunological nature and homogeneity of the HCG used.

2019 ◽  
Vol 5 (4) ◽  
pp. 69-75
Author(s):  
L. Solominova ◽  
S. Onina ◽  
G. Kozlova

The paper presents the results of the qualitative and quantitative determination of some anthocyanins (in particular, cyanidin-3-glycoside and cyanidin-3-galactoside) in the fruits of Aronia melanocarpa (Michx.) and Mahonia aquifolium (Pursh) Nutt. The reaction of extracting water-soluble dyes on the solution medium is also presented. The method of direct spectrophotometry was used. All calculations are made in the program STATISTICA 6. It is determined that the maximum content of anthocyanins is typical for fruit chokeberry Aronia. The possibility of using natural dye from chokeberry fruits in the industry is shown.


1969 ◽  
Vol 62 (1_Suppl) ◽  
pp. S95-S112 ◽  
Author(s):  
A. H. W. M. Schuurs

ABSTRACT Various techniques for sensitising erythrocytes and latex particles with gonadotrophins, particularly with HCG, are described. The haemagglutination inhibition reactions are generally interpreted by means of »erythrocyte settling patterns«. By a new method of evaluating these patterns a relatively precise quantitative determination is possible. Latex agglutination inhibition reactions on slides are particularly suitable as rapid qualitative tests. In cases where the maximum attainable sensitivity of the agglutination inhibition tests is insufficient, e. g. for determining LH concentrations in urine, the hormone in the test fluid has to be concentrated or extracted. An alternative method is a modified haemagglutination inhibition test for large volumes which is applicable to unconcentrated urine. Due to non-specific inhibitions the above-mentioned tests cannot be applied to unprocessed serum. Agglutination inhibition tests with HCG are already well advanced, pregnancy diagnosis being their main application. Now that highly purified HCG is available, a satisfactory specificity for these tests can be attained. If the immune system for HCG is used for estimating LH, it has to meet additional specificity requirements. Furthermore, the measure of cross-reaction and the choice of standard merit special attention. Finally, a literature survey is given of test systems in which LH and FSH were used as antigens.


2021 ◽  
Author(s):  
Yiwen Cao ◽  
Shenjie Zhu ◽  
Lin Zhang ◽  
Qun Cui ◽  
Haiyan Wang

Aiming at the difficulty in qualitative and quantitative analysis of trace compositions in food preservative propionic acid, the trace compositions and the key components influencing the total aldehyde content in...


1987 ◽  
Vol 20 (4) ◽  
pp. 290-291
Author(s):  
M. Moss ◽  
C.S.Y. Wong ◽  
T. McMahon ◽  
C. Cousins ◽  
C. Jacklyn

2011 ◽  
Vol 66 (1) ◽  
pp. 102-108 ◽  
Author(s):  
Shihu Wei ◽  
Peicheng Zhang ◽  
Xizhi Feng ◽  
Hiroyuki Kodama ◽  
Changyuan Yu ◽  
...  

Author(s):  
BS Dattilo ◽  
S Gallo ◽  
G Lionetti ◽  
SG Rossi

AbstractA new method is described for the qualitative and quantitative determination of both free and bound maleic hydrazide residues in tobacco leaves and cigarette filler by high performance liquid chromatography. Analyses were carried out by hydrolyzing samples of ground tobacco with 4 N hydrochloric acid for 40 minutes under reflux followed by sample chromatography, running isocratic elutions with a dilute solution of phosphoric acid. The quantitative determination of maleic hydrazide was performed by light absorption at 320 nm, by the calibration curve method. Recoveries of maleic hydrazide added to tobacco samples were greater than 90 %. The detection limit of the method, determined on ground tobacco leaves, was at least 5 ppm. The results obtained by this procedure and by the ISO standard method no. 4876 are in good accordance.


2010 ◽  
Vol 878 (29) ◽  
pp. 2943-2948 ◽  
Author(s):  
Shuli Man ◽  
Wenyuan Gao ◽  
Yanjun Zhang ◽  
Jieyin Wang ◽  
Wanshun Zhao ◽  
...  

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