Annual changes in 6-sulphatoxymelatonin excretion in man

1988 ◽  
Vol 117 (4) ◽  
pp. 470-476 ◽  
Author(s):  
Christopher J. Bojkowski ◽  
Josephine Arendt
Keyword(s):  
Intermediate Phase ◽  
Urine Samples ◽  
Plasma Samples ◽  
Annual Change ◽  
Melatonin Secretion ◽  
Normal Volunteers ◽  
Plasma Melatonin ◽  
Annual Rhythm ◽  
Major Urinary Metabolite ◽  
Annual Changes

Abstract: A recently developed RIA for 6-sulphatoxymelatonin, the major urinary metabolite of melatonin, has been used to investigate the annual change in melatonin secretion in humans. Twenty plasma samples were taken from 18 volunteers throughout a 24-h period and simultaneous 6-hourly urine samples were also collected. Plasma melatonin and urinary 6-sulphatoxymelatonin were measured by RIA. 6-Sulphatoxymelatonin assayed in the urine samples was shown to be a good index of the rhythmic characteristics of the plasma melatonin secretion. To study annual changes in excretion four sequential 6-hourly urine samples were collected at monthly intervals from 16 normal volunteers for 13 months. Cosinor curves were fitted to the 6-sulphatoxymelatonin excretion data and the 24-h rhythm was described by the cosinor parameters: amplitude, mesor and acrophase. Significant differences in the acrophase were found during the year. The summer acrophase was phase advanced relative to the winter acrophase by about 1.5 h while intermediate phase positions were observed in spring/autumn. The 24-h excretion of urinary 6-sulphatoxymelatonin was remarkably consistent and there was no annual rhythm. In contrast, the daytime 6-sulphatoxymelatonin excretion between 12.00–18.00 h showed a statistically significant seasonal rhythm, with peaks in December/January and in July.

The Effect of Moderate Weight Loss on Overnight Melatonin Secretion

1990 ◽  
Vol 156 (6) ◽  
pp. 875-877 ◽  
Author(s):  
I. M. Anderson ◽  
S. E. Gartside ◽  
P. J. Cowen
Keyword(s):  
Weight Loss ◽  
Melatonin Level ◽  
Melatonin Secretion ◽  
Plasma Melatonin ◽  
Healthy Women ◽  
Before And After

Overnight plasma melatonin level was measured in ten healthy women before and after a 4300 kJ (1000 kcaI) diet in which they lost an average 3.1 kg. This weight loss did not significantly alter melatonin levels.

Purine derivatives in spot urine samples and plasma of sheep: a possible index of microbial protein supply

1993 ◽  
Vol 1993 ◽  
pp. 5-5 ◽  
Author(s):  
X. B. Chen ◽  
Adriana T. Mejia ◽  
D. J. Kyle ◽  
E. R. Ørskov
Keyword(s):  
Urinary Excretion ◽  
Feed Intake ◽  
Urine Samples ◽  
Plasma Samples ◽  
Microbial Protein ◽  
Purine Derivatives ◽  
Feeding Regime ◽  
Spot Urine ◽  
Alternative Index ◽  
Protein Supply

In ruminants, daily urinary excretion of purine derivatives (PD) reflects the absorption of microbial purines and can be used as an index of microbial protein supply (Chen, Ørskov and Hovell, 1991). The application could be extended to farm conditions if measurements based on spot urine samples or plasma could serve as an alternative index. The objective of this study was to examine whether PD concentrations in spot urine or plasma samples vary diurnally during a given feeding regime and if they reflect differences in daily PD excretion induced by varying feed intake.

Melatonin secretion in humans assessed by measuring its metabolite, 6-sulfatoxymelatonin.

1987 ◽  
Vol 33 (8) ◽  
pp. 1343-1348 ◽  
Author(s):  
C J Bojkowski ◽  
J Arendt ◽  
M C Shih ◽  
S P Markey
Keyword(s):  
Urinary Excretion ◽  
Room Temperature ◽  
Area Under The Curve ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Plasma Samples ◽  
Melatonin Secretion ◽  
Short Pulses ◽  
Chromatographic Mass

Abstract Comparing a direct radioimmunoassay for 6-sulfatoxymelatonin (aMT6s) with an established gas chromatographic/mass spectrometric method for 6-hydroxymelatonin, we found a good correlation r = 0.94 (P less than 0.001, n = 100). aMT6s was stable, both in urine and plasma samples, without preservative, for at least two years at -20 degrees C and for five days at room temperature. Urinary excretion of aMT6s showed considerable inter-individual differences; however, the aMT6s excretion of any one individual was consistent over a four-day period, as assessed by continuous collection from 18 normal volunteers. Total 24-h urinary excretion of aMT6s was significantly correlated with the area under the curve of the respective profiles for plasma melatonin (r = 0.75, P = 0.0002) and plasma aMT6s (r = 0.70, P = 0.0005) for 22 healthy volunteers. At 24:00 h and 03:00 h, sampling plasma at 30-s intervals provided no evidence for episodic secretion (in short pulses) of either melatonin or aMT6s.

scholarly journals Polyphenol Consumption on Human Bile Acids Metabolism: Preliminary Data of Bile Acid Profiles in Human Biological Samples (P06-131-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Anqi Zhao ◽  
Xuhuiqun Zhang ◽  
Amandeep Sandhu ◽  
Indika Edirisinghe ◽  
Barbara Shukitt-Hale ◽  
...  
Keyword(s):  
Bile Acid ◽  
Qualitative Analysis ◽  
Bile Acids ◽  
Biological Samples ◽  
Urine Samples ◽  
Fragmentation Pattern ◽  
Plasma Samples ◽  
Potential Health ◽  
Human Bile ◽  
Freeze Dried

Abstract Objectives Bile acids (BAs) play a critical role in regulating human health through the activation of BAs receptor farnesoid X receptor (FXR) and membrane G protein coupled bile acid receptor-1 (TGR5). We aimed to develop methods to characterize BAs and their metabolites in human biological samples and characterize changes in BAs profile after chronic polyphenol consumption to help guide investigations on the potential health effects of polyphenols via BAs metabolism. Methods Plasma, fecal and urine samples from two human studies that included berry intake were used for developing qualitative analysis of BAs using ultra high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time of flight (QTOF). The compounds were identified based on the exact mass, fragmentation pattern, available reference standards and database search. To investigate the effect of chronic polyphenol consumption on BAs composition, pooled plasma samples (fasting and postprandial 2 h, n = 6) from a chronic (45 and 90 days) strawberry supplementation study (24 g freeze dried/day) with an older population were analyzed. Results Among 106 BAs and their metabolites which were tentatively identified in the samples used for method development, 70, 55, and 47 BAs species were characterized in plasma, feces and urine samples, respectively. The qualitative analysis of BAs in plasma samples from subjects following the strawberry consumption protocol detected 8 primary and 31 secondary BAs. After 90-days strawberry supplementation, two secondary BAs–glycolithocholic acid and 9(11), (5β)-cholenic acid-3α, 12α-diol were decreased to undetectable levels in the pooled fasting sample and the FRX/TGR5 agonists, including chenodeoxycholic acid, deoxycholic acid, cholic acid, glycodeoxycholic acid and taurocholic acid, showed increasing peak areas at 2 h postprandial compared to fasting. Conclusions The changes in BAs profiles in fasting and postprandial plasma samples after chronic strawberry feeding suggest that strawberry polyphenols may alter BAs metabolism and the FXR/TGR5 signaling. Funding Sources This work was funded by the California Strawberry Commission, USDA Intramural Funds and various donor funds to the Center for Nutrition Research, IIT.

Melatonin in rat milk and the likelihood of its role in postnatal maternal entrainment of rhythms

2002 ◽  
Vol 282 (3) ◽  
pp. R797-R804 ◽  
Author(s):  
Shawn A. Rowe ◽  
David J. Kennaway
Keyword(s):  
Peak Plasma ◽  
Subcutaneous Administration ◽  
Melatonin Secretion ◽  
Plasma Melatonin ◽  
Rat Pups ◽  
Propranolol Administration ◽  
Maternal Entrainment

The rhythm of melatonin in rat milk and the capacity of pups to synthesize and metabolize melatonin were studied. Melatonin was undetectable in milk in the light (<21 pM), but increased rapidly 2–4 h after dark to peak at 357 ± 66 pM at mid-dark. Oral or subcutaneous administration of melatonin to 5- and 10-day-old pups resulted in peak plasma melatonin levels 30 min after administration and rapid metabolism. Increases in pineal and plasma melatonin levels at night were detected at 5 and 6 days of age, respectively. Isoproterenol administration (2 μg/g body wt) at mid-light to day 10 pups increased plasma melatonin from 312 ± 40 pM to 1,298 ± 160 pM, whereas propranolol (2 μg/g body wt) suppressed nocturnal melatonin secretion from 1,270 ± 128 pM to 395 ± 66 pM. The rise of pineal and plasma melatonin in day 10 pups occurred 1 and 2 h after dark onset, respectively, preceding the onset in dams by 3 and 4 h, respectively. Propranolol administration to 2- and 5-day lactating dams inhibited plasma and milk melatonin at night but had no effect on their suckling pups. Transfer of melatonin via the milk is unlikely to provide an entraining signal for rat pups.

scholarly journals Investigation of the individual human sulfatome in plasma and urine samples reveals an age-dependency

RSC Advances ◽  
2021 ◽  
Vol 11 (55) ◽  
pp. 34788-34794
Author(s):  
Mário S. P. Correia ◽  
Bhawana Thapa ◽  
Miroslav Vujasinovic ◽  
J.-Matthias Löhr ◽  
Daniel Globisch
Keyword(s):  
Urine Samples ◽  
Plasma Samples ◽  
Age Dependency ◽  
Plasma And Urine Samples ◽  
The Individual ◽  
Individual Human

Profile of the human core sulfatome in urine and plasma samples.

scholarly journals Comparison of the Metabolic Profiles in the Plasma and Urine Samples Between Autistic and Typically Developing Boys: A Preliminary Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Xin-Jie Xu ◽  
Xiao-E Cai ◽  
Fan-Chao Meng ◽  
Tian-Jia Song ◽  
Xiao-Xi Wang ◽  
...  
Keyword(s):  
Autism Spectrum ◽  
Urine Samples ◽  
Metabolic Profiles ◽  
Plasma Samples ◽  
Typically Developing ◽  
Children With Asd ◽  
Full Picture ◽  
Plasma And Urine Samples ◽  
Healthy Control ◽  
Age Range

Background: Autism spectrum disorder (ASD) is defined as a pervasive developmental disorder which is caused by genetic and environmental risk factors. Besides the core behavioral symptoms, accumulated results indicate children with ASD also share some metabolic abnormalities.Objectives: To analyze the comprehensive metabolic profiles in both of the first-morning urine and plasma samples collected from the same cohort of autistic boys.Methods: In this study, 30 autistic boys and 30 tightly matched healthy control (HC) boys (age range: 2.4~6.7 years) were recruited. First-morning urine and plasma samples were collected and the liquid chromatography–mass spectrometry (LC-MS) was applied to obtain the untargeted metabolic profiles. The acquired data were processed by multivariate analysis and the screened metabolites were grouped by metabolic pathway.Results: Different discriminating metabolites were found in plasma and urine samples. Notably, taurine and catechol levels were decreased in urine but increased in plasma in the same cohort of ASD children. Enriched pathway analysis revealed that perturbations in taurine and hypotaurine metabolism, phenylalanine metabolism, and arginine and proline metabolism could be found in both of the plasma and urine samples.Conclusion: These preliminary results suggest that a series of common metabolic perturbations exist in children with ASD, and confirmed the importance to have a comprehensive analysis of the metabolites in different biological samples to reveal the full picture of the complex metabolic patterns associated with ASD. Further targeted analyses are needed to validate these results in a larger cohort.

scholarly journals Virus shedding patterns in nasopharyngeal and fecal specimens of COVID-19 patients

2020 ◽  
Author(s):  
Ning Zhang ◽  
Yuhuan Gong ◽  
Fanping Meng ◽  
Yuhai Bi ◽  
Penghui Yang ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Patient Discharge ◽  
Urine Samples ◽  
Virus Dynamics ◽  
Plasma Samples ◽  
Virus Shedding ◽  
Detection Point ◽  
Plasma And Urine Samples ◽  
Discharged Patients ◽  
Upper Respiratory

Diagnosis is the key point for confirmation and treatment of COVID-19. we focused on comparative analysis of virus dynamics between the upper respiratory and feces specimens in the COVID-19 patients. A total of 66 upper respiratory swabs, 51 feces, 56 urine and 56 plasma samples were sequentially collected from 23 patients in a designated hospital. The plasma and urine samples were all negative, except for urine samples from two severe cases at the latest available detection point. Conversely, virus was shed in respiratory swabs and feces samples during the diseased period. Ten of 12 (83.3%) cases were positive for feces samples, while 14 of 21 (66.7%) were positive for respiratory samples. In addition, the median duration of virus shedding was 10.0 days (IQR 8.0 to 17.0) in the upper respiratory swabs, but was 22.0 days (IQR 15.5 to 23.5) for the feces. Notably, at 26 days after discharge, case 3 (a 45-year-old) was detected positive again in the feces samples, but appears to be healthy and negative for respiratory swabs. These results indicated that beside respiratory samples, intestinal samples (e.g. feces) should be recommended for diagnosis of COVID-19, especially before a patient discharge and for monitoring the relapse of discharged patients.

Inhibition of nighttime melatonin secretion in cattle: threshold light intensity for dairy heifers

2001 ◽  
Vol 81 (1) ◽  
pp. 153-156 ◽  
Author(s):  
T. J. Lawson ◽  
A. D. Kennedy
Keyword(s):  
Light Intensity ◽  
Key Words ◽  
Latin Square ◽  
Exposure Period ◽  
Melatonin Secretion ◽  
Melatonin Concentration ◽  
Dairy Heifers ◽  
Plasma Melatonin ◽  
Light Intensities ◽  
Lower Light

Five pre-pubertal Holstein heifers were exposed for 16 h to a light intensity of 400 lx and thereafter to intensities of 0, 50, 100, 200, or 400 lx for an additional 8 h (5 x 5 Latin square design). Exposure to all intensities inhibited (P < 0.05) melatonin secretion for the initial few hours (of the 8-h exposure period); melatonin concentration subsequently increased, particularly with the lower light intensities. Only the highest light intensity (400 lx) suppressed (P < 0.05) plasma melatonin concentrations for the entire 8-h exposure period. Key words: Threshold, supplemental, light, intensity, inhibition, melatonin, heifer

scholarly journals Genetic variability in melatonin secretion originates in the number of pinealocytes in sheep

2002 ◽  
Vol 172 (2) ◽  
pp. 397-404 ◽  
Author(s):  
A Gomez Brunet ◽  
A Gomez Brunet ◽  
B Malpaux ◽  
A Daveau ◽  
C Taragnat ◽  
...  
Keyword(s):  
Genetic Variability ◽  
Pineal Gland ◽  
Melatonin Secretion ◽  
High Group ◽  
Plasma Melatonin ◽  
Genetic Groups ◽  
Mean Size ◽  
The Mean ◽  
Genetic Value ◽  
The Brain

Genetic variability in plasma melatonin concentrations in ewes results from variations in pineal weight. This study investigated whether it is due to a difference in the number of pinealocytes, or in their size. Two groups of lambs were assigned before birth to being extremes (18 High and 21 Low) by calculating their genetic value on the basis of the melatonin concentrations of their parents. Lambs were bled from 1 week of age until 14 weeks of age. Pineal gland, brain and pituitary weights, length and width of the brain, and length of the hypothalamus were recorded. A significant effect (ANOVA) of genetic group (P<0.05) and age (P<0.05) was detected on mean nocturnal plasma melatonin concentrations, as soon as the first week after birth (mean +/- s.e.m.; High: 51.7 +/- 10.7 vs Low: 31.9 +/- 3.2 pg/ml). There was no difference between the two genetic groups in any of the brain parameters measured, but the pineal glands of the High group were heavier and contained significantly more pinealocytes (High: 27.8 +/- 2.4 vs Low: 21.0 +/- 2.4 x 10(6); P<0.05) than those in the Low group. The mean size of pinealocytes did not differ between the two genetic groups. Thus, the genetic variability in nocturnal plasma melatonin concentrations in sheep is expressed by 1 week of age and higher levels of secretion are the consequence of larger pineal glands containing a greater number of pinealocytes.

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