Regulation of bovine and ovine lymphocyte proliferation by progesterone: modulation by steroid receptor antagonists and physiological status

1993 ◽  
Vol 129 (6) ◽  
pp. 532-535 ◽  
Author(s):  
Victor H Monterroso ◽  
Peter J Hansen
Keyword(s):  
Lymphocyte Proliferation ◽  
Steroid Receptors ◽  
Steroid Receptor ◽  
Physiological Status ◽  
Inhibitory Effects ◽  
Receptor Antagonists ◽  
Lactating Cows

Lectin-stimulated sheep and cow lymphocytes were used to test whether inhibitors of classical steroid receptors block suppressive effects of progesterone and whether effects of progesterone vary with physiological status. Neither RU 38486 nor RU 43044 blocked the inhibitory effects of progesterone on lymphocyte proliferation. Rather, these antagonists were themselves inhibitory. Effects of progesterone and antagonists were additive: the percentage inhibition caused by progesterone was similar whether antagonists were present or not. The degree of lymphocyte proliferation and the inhibitory effects of progesterone were of the same magnitude for pregnant/lactating cows, pregnant/non-lactating cows, postpartum/lactating cows and cyclic/non-lactating cows. In conclusion, progesterone does not appear to inhibit lymphocyte proliferation through actions that involve classical steroid receptors. There was no evidence that lymphocyte proliferation in the cow is suppressed during pregnancy or that the inhibitory effects of progesterone increase during pregnancy.

Inhibitory effects of cucurbitacin R on lymphocyte proliferation and cytokine production

Planta Medica ◽  
2006 ◽  
Vol 72 (11) ◽  
Author(s):  
JM Escandell ◽  
MC Recio ◽  
R Gil ◽  
I Merfort ◽  
JL Ríos
Keyword(s):  
Lymphocyte Proliferation ◽  
Cytokine Production ◽  
Inhibitory Effects

scholarly journals Expression of steroid receptor coactivators and corepressors in human endometrial hyperplasia and carcinoma with relevance to steroid receptors and Ki-67 expression

Cancer ◽  
2003 ◽  
Vol 98 (10) ◽  
pp. 2207-2213 ◽  
Author(s):  
Junko Uchikawa ◽  
Tanri Shiozawa ◽  
Hsien-Chang Shih ◽  
Tsutomu Miyamoto ◽  
Yu-Zhen Feng ◽  
...  
Keyword(s):  
Endometrial Hyperplasia ◽  
Steroid Receptors ◽  
Steroid Receptor ◽  
Ki 67 ◽  
Steroid Receptor Coactivators

scholarly journals Meeting the physiological requirements for energy and nutrients to manifest the genetic potential of dairy cattle

2021 ◽  
Vol 941 (1) ◽  
pp. 012036
Author(s):  
S V Moshkina ◽  
A S Kharitonova ◽  
S N Khimicheva ◽  
T Yu Kolganova ◽  
T V Smagina ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Control Group ◽  
Physiological Status ◽  
Feed Supplement ◽  
Dry Period ◽  
Brown Swiss ◽  
Lactating Cows ◽  
Economic Experiment ◽  
Genetic Potential ◽  
Research Period

Abstract The paper presents the results of the scientific and economic experiment for the evaluation of the influence of the energy supplement included in the diet of lactating cows of the Brown Swiss breed. During the research period, all animals were kept in the same conditions, corresponding to zootechnical standards. They were divided into two groups differentiated only by the feed ration. The cows of the first (control) group were given rations typical for the farm corresponding to the feeding norms of dry and lactating cows. For the cows of the second group in the dry period (14 days before calving) the feeding ration was changed with a change in structure towards an increase in the proportion of concentrated feed, and immediately after calving, they received an energy feed supplement to maintain the physiological status of dairy cows after calving. Only clinically healthy animals participated in the experiment. It was revealed that the inclusion of the energy feed supplement in the diet of lactating cows in the experimental groups during lactation led to an increase in the productivity of livestock as compared with those in the control group.

scholarly journals The Effects of Endocrine Disrupting Chemicals on Biomarkers of Inflammation Produced by Lipopolysaccharide Stimulated RAW264.7 Macrophages

2019 ◽  
Vol 16 (16) ◽  
pp. 2914
Author(s):  
Vedastus W. Makene ◽  
Edmund J. Pool
Keyword(s):  
Estrogen Receptor ◽  
Receptor Antagonist ◽  
Cell Cultures ◽  
Endocrine Disrupting Chemicals ◽  
Raw264.7 Cells ◽  
Inhibitory Effects ◽  
Receptor Antagonists ◽  
Estrogen Receptor Antagonist ◽  
Endocrine Disrupting ◽  
Raw264.7 Cell

Endocrine disrupting chemicals (EDCs) are common pollutants in the environment and can induce disruption of the endocrine and immune systems. The present study evaluated the effects of selected common environmental EDCs on secretion of inflammatory biomarkers by RAW264.7 cells. The EDCs investigated were Estradiol (E2), 5α-dihydrotestosterone (DHT), and Bisphenol A (BPA). To evaluate if the effects caused by EDCs were modulated by steroid hormone receptors, antagonists of estrogen and androgen receptors were used. The steroid receptor antagonists used were Tamoxifen, an estrogen receptor antagonist, and Flutamide, an androgen receptor antagonist. Secretion of biomarkers of inflammation, namely nitric oxide (NO) and interleukin 6 (IL-6), were monitored. The NO was determined using Griess reaction and IL-6 was measured by enzyme linked immunosorbent assay (ELISA). Although 5 μg/mL E2, DHT, and BPA were not toxic to RAW264.7 cell cultures, the same treatments significantly (p < 0.001) reduced both NO and IL-6 secretion by lipopolysaccharide (LPS)-stimulated RAW264.7 cell cultures. The suppression of NO and IL-6 secretion indicate inhibition of inflammation by DHT, E2, and BPA. The inhibitory effects of DHT, E2 and BPA are partially mediated via their cellular receptors, because the effects were reversed by their respective receptor antagonists. Flutamide reversed the effects of DHT, while Tamoxifen reversed the effects of E2 and BPA. In conclusion, E2, BPA, and DHT inhibit the synthesis of inflammation biomarkers by LPS-stimulated RAW264.7 cells. The inhibitory effects of EDCs can be partially reversed by the addition of an estrogen receptor antagonist for E2 and BPA, and an androgenic receptor antagonist for DHT. The inhibition of inflammatory response in stimulated RAW264.7 cells may be a useful bioassay model for monitoring estrogenic and androgenic pollutants.

scholarly journals Inhibition of human lymphocyte proliferation by monoclonal antibody to transferrin receptor

Blood ◽  
1983 ◽  
Vol 62 (4) ◽  
pp. 821-826 ◽  
Author(s):  
J Mendelsohn ◽  
I Trowbridge ◽  
J Castagnola
Keyword(s):  
Cell Cycle ◽  
Monoclonal Antibody ◽  
Lymphocyte Proliferation ◽  
Human Lymphocytes ◽  
Nitrilotriacetic Acid ◽  
S Phase ◽  
Inhibitory Effects ◽  
Mitogenic Response ◽  
Cell Cycle Inhibition ◽  
Partial Reversal

Abstract A monoclonal antibody, 42/6, which blocks the binding of transferrin to its receptor on the cell membrane, inhibits proliferation of human lymphocytes stimulated by phytohemagglutinin. Anti-receptor antibody B3/25, which does not block transferrin binding, does not alter the mitogenic response. Addition of soluble iron, in the form of ferric nitrilotriacetic acid, results in partial reversal of inhibition. Lymphocytes in the quiescent phase of the cell cycle at the time of 42/6 antibody addition are unable to traverse S phase, whereas cells actively proliferating when antibody is added are sensitive to its inhibitory effects throughout all phases of the cell cycle. Inhibition is static rather than cidal, since it can be reversed by removal of antibody after up to 48 hr of exposure.

A new approach to the quality control of steroid receptor assays.

1988 ◽  
Vol 34 (10) ◽  
pp. 2076-2079
Author(s):  
M Borsotti ◽  
M Quercioli ◽  
R D'Ettole ◽  
A Piffanelli ◽  
G Messeri
Keyword(s):  
Quality Control ◽  
Sample Preparation ◽  
Steroid Receptors ◽  
Menopausal Status ◽  
Steroid Receptor ◽  
Usual Practice ◽  
New Approach ◽  
Breast Tumor Tissue ◽  
Consensus Mean ◽  
Receptor Assays

Abstract Lyophilized cytosols prepared from calf uterus and human breast tumor tissue are commonly used to assess the reliability of routine steroid receptor assays. However, preanalytical error (sample preparation, storage, homogenization) cannot be detected in this way. Participating laboratories were asked to mail us all their receptor results obtained over a four-month interval, and to include some information about the patients involved (age, menopausal status, nodal status). After verifying the homogeneity of the populations investigated, we computed consensus means for the percentages of positive samples and for their absolute value. Despite the homogeneity of the characteristics of the populations studied, results from some laboratories systematically differed from the consensus mean. This approach to the quality control of steroid receptors allows evaluation of the whole procedure, from sample preparation to analysis, and thus may be helpful as an addition to the usual practice of distribution of cytosols and tissue powders for assay.

scholarly journals Energy and lipid metabolism gene expression of D18 embryos in dairy cows is related to dam physiological status

2014 ◽  
Vol 46 (2) ◽  
pp. 39-56 ◽  
Author(s):  
D. Valour ◽  
S. A. Degrelle ◽  
A. A. Ponter ◽  
C. Giraud-Delville ◽  
E. Campion ◽  
...  
Keyword(s):  
Gene Expression ◽  
Lipid Synthesis ◽  
Physiological Status ◽  
Molecular Characteristics ◽  
Morphological Development ◽  
Continue Development ◽  
Nonesterified Fatty Acids ◽  
Lactating Cows ◽  
Lipid Metabolism Gene ◽  
Acetate Utilization

We analyzed the change in gene expression related to dam physiological status in day (D)18 embryos from growing heifers (GH), early lactating cows (ELC), and late lactating cows (LLC). Dam energy metabolism was characterized by measurement of circulating concentrations of insulin, glucose, IGF-1, nonesterified fatty acids, β-hydroxybutyrate, and urea before embryo flush. The metabolic parameters were related to differential gene expression in the extraembryonic tissues by correlation analysis. Embryo development estimated by measuring the length of the conceptuses and the proportion of expected D18 gastrulating stages was not different between the three groups of females. However, embryo metabolism was greatly affected by dam physiological status when we compared GH with ELC and GH with LLC but to a lesser extent when ELC was compared with LLC. Genes involved in glucose, pyruvate, and acetate utilization were upregulated in GH vs. ELC conceptuses (e.g., SLC2A1, PC, ACSS2, ACSS3). This was also true for the pentose pathway ( PGD, TKT), which is involved in synthesis of ribose precursors of RNA and DNA. The pathways involved in lipid synthesis were also upregulated in GH vs. ELC. Despite similar morphological development, the molecular characteristics of the heifers' embryos were consistently different from those of the cows. Most of these differences were strongly related to metabolic/hormone patterns before insemination and during conceptus free-life. Many biosynthetic pathways appeared to be more active in heifer embryos than in cow embryos, and consequently they seemed to be healthier, and this may be more conducive to continue development.

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