scholarly journals Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2α-induced regression in sheep

Reproduction ◽  
2006 ◽  
Vol 131 (6) ◽  
pp. 1115-1126 ◽  
Author(s):  
Kimberly A Vonnahme ◽  
Dale A Redmer ◽  
Ewa Borowczyk ◽  
Jerzy J Bilski ◽  
Justin S Luther ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Growth Factor ◽  
Mrna Expression ◽  
Collagen Type ◽  
Angiogenic Factors ◽  
Cell Marker ◽  
Luteal Regression ◽  
Endothelial Cell Marker ◽  
Collagen Type 1

Corpora lutea and blood samples were collected from superovulated ewes 0, 4, 8, 12 and 24 h after prostaglandin F2α(PGF) analog injection on day 10 of the estrous cycle. Changes in vascular cell and fibroblast composition, apoptosis and mRNA expression for several angiogenic factors in the corpus luteum (CL) were determined. While peripheral progesterone concentration decreased at 24 h after PGF injection, CL weight did not change. The area of positive BS-1 lectin staining (endothelial cell marker), smooth muscle cell actin (SMCA; pericyte and SMC marker), collagen type 1 (fibroblast marker), and the rate of cell death changed in luteal tissues after PGF treatment. In association with these cellular changes, mRNA for several angiogenic factors including vascular endothelial growth factor (VEGF) and receptors (FltandKDR), basic fibroblast growth factor (FGF2) and receptor,angiopoietin (ANGPT) 1and receptorTie-2,endothelial nitric oxide synthase(NOS3), andangiotensin II receptor 1(AT1) were altered. Changes in endothelial cell marker expression were positively correlated with changes in VEGF and NO systems. In addition, changes in mRNA expression forVEGF,FltandKDRwere positively correlated with changes inANGPT2,Tie-2, andNOS3,indicating a functional relationship. This data demonstrates that after an initial increase, the endothelial component of the vascular bed decreases during PGF-induced luteal regression. However, SMCA expression remained high during luteal regression, potentially indicating a role of pericytes and vascular SMC in luteolysis, likely to regulate tissue remodeling and to maintain the integrity of larger blood vessels. Further, it appears that early regression may increase collagen type 1 production and/or expression by fibroblasts. Expression of angiogenic factors is influenced by PGF-induced luteolysis and may serve to maintain vascular structure in order to aid luteal regression.

Evidence for the interaction of fibroblast growth factor-2 with the lymphatic endothelial cell marker LYVE-1

Blood ◽  
2013 ◽  
Vol 121 (7) ◽  
pp. 1229-1237 ◽  
Author(s):  
Natalia Platonova ◽  
Geraldine Miquel ◽  
Birgit Regenfuss ◽  
Said Taouji ◽  
Claus Cursiefen ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Lymphatic Endothelial Cell ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Cell Marker ◽  
Endogenous Expression ◽  
Key Points ◽  
Endothelial Cell Marker

Key Points FGF2 is able to directly interact with LYVE-1 and glycosylation of LYVE-1 is important for the interaction with FGF2. LYVE-1 inhibits FGF2-dependent lymphangiogenesis and FGF2 modulates LYVE-1's endogenous expression and reverses the effect of TNFβ.

scholarly journals Omidenepag, a non-prostanoid EP2 receptor agonist, induces enlargement of the 3D organoid of 3T3-L1 cells

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Yosuke Ida ◽  
Fumihito Hikage ◽  
Araya Umetsu ◽  
Haruka Ida ◽  
Hiroshi Ohguro
Keyword(s):  
Mrna Expression ◽  
Receptor Agonist ◽  
Collagen Type ◽  
Physical Property ◽  
Upper Eyelid ◽  
3D Cultures ◽  
Ep2 Receptor ◽  
Collagen Type 1 ◽  
2D And 3D

Abstract 2D and 3D cultures of 3T3-L1 cells were employed in a study of the effects of Omidenepag (OMD), interacting with a non-prostanoid EP2 receptor, on adipogenesis. Upon adipogenesis, the effects on lipid staining, the mRNA expression of adipogenesis-related genes (Pparγ, CEBPa, Ap2, and Glut4) and the extracellular matrix (ECM) including collagen type 1, 4 and 6, and fibronectin, and the size and physical property of 3D organoids were compared between groups that had been treated with EP2 agonists (butaprost and OMD) and PGF2α. Upon adipogenesis, these significantly suppressed lipid staining and the mRNA expression of related genes. EP2 agonists and PGF2α influenced the mRNA expression of ECM in different manners, and these effects were also different between 2 and 3D cultures. Examining the physical properties by a microsqueezer indicated that the solidity of the 3D organoids became significantly lowered upon adipogenesis and these effects were not affected by EP2 agonists. In contrast, 3D organoid stiffness was markedly enhanced by the presence of PGF2α. These observations indicate that EP2 agonists affect the adipogenesis of 3T3-L1 cells in different manners, as compared to PGF2α, suggesting that OMD may not induce PGF2α related orbital fat atrophy, called the deepening of the upper eyelid sulcus (DUES).

MMP-2 Inhibition Attenuates IL-13 Induced Collagen Type-1 mRNA Expression in Human Airway Fibroblasts in Asthma

2011 ◽  
Vol 127 (2) ◽  
pp. AB127-AB127
Author(s):  
R. Firszt ◽  
T.D. Church ◽  
D. Francisco ◽  
D. Beaver ◽  
J.L. Ingram ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Collagen Type ◽  
Human Airway ◽  
Collagen Type 1

scholarly journals Direct comparison of 3D and 2D cultivation reveals higher osteogenic capacity of elderly osteoblasts in 3D

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Stephan Payr ◽  
Elizabeth Rosado-Balmayor ◽  
Thomas Tiefenboeck ◽  
Tim Schuseil ◽  
Marina Unger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Collagen Type ◽  
3D Culture ◽  
Osteogenic Potential ◽  
Donor Age ◽  
Collagen Type 1 ◽  
2D And 3D ◽  
Human Primary Osteoblasts ◽  
Gene Expression Levels

Abstract Background The aim of this study was the investigation of the osteogenic potential of human osteoblasts of advanced donor age in 2D and 3D culture. Methods Osteoblasts were induced to osteogenic differentiation and cultivated, using the same polystyrene material in 2D and 3D culture for 2 weeks. Samples were taken to evaluate alkaline phosphatase (ALP) activity, mineralization and gene expression. Results Osteoprotegerin (OPG) levels were significantly increased (8.2-fold) on day 7 in 3D compared to day 0 (p < 0.0001) and 11.6-fold higher in 3D than in 2D (p < 0.0001). Both culture systems showed reduced osteocalcin (OC) levels (2D 85% and 3D 50% of basic value). Collagen type 1 (Col1) expression was elevated in 3D on day 7 (1.4-fold; p = 0.009). Osteopontin (OP) expression showed 6.5-fold higher levels on day 7 (p = 0.002) in 3D than in 2D. Mineralization was significantly higher in 3D on day 14 (p = 0.0002). Conclusion Advanced donor age human primary osteoblasts reveal significantly higher gene expression levels of OPG, Col1 and OP in 3D than in monolayer. Therefore, it seems that a relatively high potential of bone formation in a natural 3D arrangement is presumably still present in osteoblasts of elderly people. Trial registration 5217/11 on the 22nd of Dec. 2011.

scholarly journals Evaluation of Collagen Type-1 Production and Anti-Inflammatory Activities of Human Placental Extracts in Human Gingival Fibroblasts

2016 ◽  
Vol 25 (3) ◽  
pp. 277-281 ◽  
Author(s):  
Homare Akagi ◽  
Yasuhiro Imamura ◽  
Yoshimasa Makita ◽  
Hiroe Nakamura ◽  
Naomi Hasegawa ◽  
...  
Keyword(s):  
Collagen Type ◽  
Human Gingival Fibroblasts ◽  
Gingival Fibroblasts ◽  
Anti Inflammatory ◽  
Collagen Type 1
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