Activity-Dependent A-to-I RNA Editing in Rat Cortical Neurons

The spatial distribution of neurons and activity-dependent neurite outgrowth shape long-range interaction, recurrent local connectivity and the modularity in neuronal networks. We investigated how this mesoscale architecture develops by interaction of neurite outgrowth, cell migration and activity in cultured networks of rat cortical neurons and show that simple rules can explain variations of network modularity. In contrast to theoretical studies on activity-dependent outgrowth but consistent with predictions for modular networks, spontaneous activity and the rate of synchronized bursts increased with clustering, whereas peak firing rates in bursts increased in highly interconnected homogeneous networks. As Ca2+ influx increased exponentially with increasing network recruitment during bursts, its modulation was highly correlated to peak firing rates. During network maturation, long-term estimates of Ca2+ influx showed convergence, even for highly different mesoscale architectures, neurite extent, connectivity, modularity and average activity levels, indicating homeostatic regulation towards a common set-point of Ca2+ influx.

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The Protective Effects of Jatrorrhizine on β-Amyloid (25-35)-Induced Neurotoxicity in Rat Cortical Neurons

CNS & Neurological Disorders - Drug Targets ◽

10.2174/1871527311211080013 ◽

2013 ◽

Vol 11 (8) ◽

pp. 1030-1037 ◽

Cited By ~ 17

Author(s):

Tao Luo ◽

Wei Jiang ◽

Yan Kong ◽

Sheng Li ◽

Feng He ◽

...

Keyword(s):

Cortical Neurons ◽

Protective Effects ◽

Rat Cortical Neurons ◽

Β Amyloid

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The Bacterial Enzyme Cas13 Interferes with Neurite Outgrowth from Cultured Cortical Neurons

Toxins ◽

10.3390/toxins13040262 ◽

2021 ◽

Vol 13 (4) ◽

pp. 262

Author(s):

Qin-Wei Wu ◽

Josef P. Kapfhammer

Keyword(s):

Rna Editing ◽

Cortical Neurons ◽

High Efficiency ◽

Primary Cultures ◽

Cultured Neurons ◽

Therapeutic Tool ◽

Bacterial Enzyme ◽

Rna Targeting ◽

Cultured Cortical Neurons ◽

New Generation

The CRISPR-Cas13 system based on a bacterial enzyme has been explored as a powerful new method for RNA manipulation. Due to the high efficiency and specificity of RNA editing/interference achieved by this system, it is currently being developed as a new therapeutic tool for the treatment of neurological and other diseases. However, the safety of this new generation of RNA therapies is still unclear. In this study, we constructed a vector expressing CRISPR-Cas13 under a constitutive neuron-specific promoter. CRISPR-Cas13 from Leptotrichia wadei was expressed in primary cultures of mouse cortical neurons. We found that the presence of CRISPR-Cas13 impedes the development of cultured neurons. These results show a neurotoxic action of Cas13 and call for more studies to test for and possibly mitigate the toxic effects of Cas13 enzymes in order to improve CRISPR-Cas13-based tools for RNA targeting.

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Inhibition by inorganic ions of a sustained calcium signal evoked by activation of mGlu5 receptors in rat cortical neurons and glia

British Journal of Pharmacology ◽

10.1038/sj.bjp.0702203 ◽

1998 ◽

Vol 125 (7) ◽

pp. 1551-1561 ◽

Cited By ~ 16

Author(s):

Larissa S Prothero ◽

Christopher D Richards ◽

Alistair Mathie

Keyword(s):

Cortical Neurons ◽

Inorganic Ions ◽

Calcium Signal ◽

Rat Cortical Neurons ◽

Mglu5 Receptors

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Human group IIA secretory phospholipase A2 potentiates Ca2+ influx through L-type voltage-sensitive Ca2+ channels in cultured rat cortical neurons

Journal of Neurochemistry ◽

10.1046/j.1471-4159.2003.01712.x ◽

2003 ◽

Vol 85 (3) ◽

pp. 749-758 ◽

Cited By ~ 48

Author(s):

Tatsurou Yagami ◽

Keiichi Ueda ◽

Kenji Asakura ◽

Hitoshi Nakazato ◽

Satoshi Hata ◽

...

Keyword(s):

Phospholipase A2 ◽

Cortical Neurons ◽

Secretory Phospholipase A2 ◽

Human Group ◽

Secretory Phospholipase ◽

Rat Cortical Neurons ◽

Ca2 Channels

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Comparative AAV-eGFP Transgene Expression Using Vector Serotypes 1–9, 7m8, and 8b in Human Pluripotent Stem Cells, RPEs, and Human and Rat Cortical Neurons

Stem Cells International ◽

10.1155/2019/7281912 ◽

2019 ◽

Vol 2019 ◽

pp. 1-11 ◽

Cited By ~ 6

Author(s):

Thu T. Duong ◽

James Lim ◽

Vidyullatha Vasireddy ◽

Tyler Papp ◽

Hung Nguyen ◽

...

Keyword(s):

Cortical Neurons ◽

Transgene Expression ◽

Fluorescent Protein ◽

Ex Vivo ◽

Cell Types ◽

Egfp Expression ◽

Cell Type ◽

Egfp Gene ◽

Rat Cortical Neurons

Recombinant adeno-associated virus (rAAV), produced from a nonpathogenic parvovirus, has become an increasing popular vector for gene therapy applications in human clinical trials. However, transduction and transgene expression of rAAVs can differ acrossin vitroand ex vivo cellular transduction strategies. This study compared 11 rAAV serotypes, carrying one reporter transgene cassette containing a cytomegalovirus immediate-early enhancer (eCMV) and chicken beta actin (CBA) promoter driving the expression of an enhanced green-fluorescent protein (eGFP) gene, which was transduced into four different cell types: human iPSC, iPSC-derived RPE, iPSC-derived cortical, and dissociated embryonic day 18 rat cortical neurons. Each cell type was exposed to three multiplicity of infections (MOI: 1E4, 1E5, and 1E6 vg/cell). After 24, 48, 72, and 96 h posttransduction, GFP-expressing cells were examined and compared across dosage, time, and cell type. Retinal pigmented epithelium showed highest AAV-eGFP expression and iPSC cortical the lowest. At an MOI of 1E6 vg/cell, all serotypes show measurable levels of AAV-eGFP expression; moreover, AAV7m8 and AAV6 perform best across MOI and cell type. We conclude that serotype tropism is not only capsid dependent but also cell type plays a significant role in transgene expression dynamics.

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