scholarly journals Bioconversion of cassava stem to ethanol using Aspergillus fumigatus and Saccharomyces cerevisiae

BioResources ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. 6895-6908
Author(s):  
Bo Yu ◽  
Luqiao Jin ◽  
Huiling Xia ◽  
Yu Lu ◽  
Mengyi Dong
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Aspergillus Fumigatus ◽  
Ethanol Production ◽  
Alkaline Solution ◽  
Rotational Speed ◽  
Substrate Concentration ◽  
Optimum Conditions ◽  
Fermentation Time ◽  
Initial Ph ◽  
Cassava Stem

Cassava stem was bioconverted to ethanol using microorganisms. First, cassava stem was pretreated by in ways, alkaline solution alone (ASA), microwave treatment combined with alkaline solution (MTCAS), and ultrasonic treatment combined with alkaline solution (UTCAS). The compositions of cassava stem pretreated by different methods were analyzed, and the results showed that the cassava stem pretreated by MTCAS was more suitable for saccharification and subsequent ethanol production. The pretreated cassava stem was subjected to simultaneous saccharification and ethanol production using Aspergillus fumigatus and Saccharomyces cerevisiae. Response surface methodology was used to optimize various process parameters including fermentation temperature, initial pH, fermentation time, rotational speed and substrate concentration. A bioconversion yield of 70 mg/g was obtained at the optimum conditions of fermentation, viz, temperature 35 °C, initial pH 5.6, fermentation time 132 h, rotational speed 155 rpm, and substrate concentration 4.6 wt%. An experiment under optimum conditions confirmed the model predictions. The results suggest that pretreatment with MTCAS and simultaneous fermentation with A. fumigatus and S. cerevisiae would be a good choice for the bioconversion of lignocellulosic biomass to bioethanol. Considering the cost advantage, using microbial fermentation instead of pure enzyme hydrolysis is more advantageous in 2nd generation bioethanol production.

scholarly journals PRODUCTION OF BIOETHANOL FROM RICE STRAW USING YEAST EXTRACTS PEPTONE DEXTROSE

2016 ◽  
Vol 36 (1) ◽  
pp. 296-301
Author(s):  
DY Tsunatu ◽  
KG Atiku ◽  
TT Samuel ◽  
BI Hamidu ◽  
DI Dahutu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Particle Size ◽  
Rice Straw ◽  
Substrate Concentration ◽  
Ph Value ◽  
Lignin Content ◽  
Treatment Method ◽  
Optimum Conditions ◽  
Fermentation Time ◽  
Pre Treatment

The production of bio-ethanol from Rice Straw (Oryza sativa) was carried out using rice straw as a feedstock and a combination of Yeast Extracts Peptone Dextrose (YEPD)at 0.2%(w/v) 0.4%(w/v), 0.6%(w/v), 0.8%(w/v) and 1%(w/v) concentrations and Saccharomyces cerevisiae (yeast) at 0.5% (w/v), 1%(w/v), 1.5%(w/v), 2%(w/v) and 2.5%(w/v) concentrations as cells for fermentation. The study determined the most suitable pre-treatment method from the following pretreatment methods; 1M NaOH and heating. IM NaOH pre-treatment gave the highest cellulose and lowest lignin content. The effects of substrate concentration values of 1g/l, 2g/l, 4g/l, 6g/l and 8g/l; with particle size of 300μm and cell loading combination of YEPD at 0.2%(w/v) 0.4%(w/v), 0.6%(w/v), 0.8%(w/v), 1%(w/v) concentrations and Saccharomyces cerevisiae (yeast) at 0.5% (w/v), 1%(w/v), 1.5%(w/v), 2%(w/v), 2.5%(w/v) on the fermentation process were investigated to obtain optimum conditions of fermentation. The optimum conditions of fermentation were obtained at temperature of 330C, pH value of 4.0, substrate concentration of 4g/l, particle size 300μm and YEPD to yeast ratio of 0.8/1.5 after 72 hours of fermentation time. Also substrate concentration of 4g/l, gave highest bioethanol yield of 49.50%. http://dx.doi.org/10.4314/njt.v36i1.36

Bioethanol Production from Pineapple Peel Juice Using Saccharomyces cerevisiae

2014 ◽  
Vol 875-877 ◽  
pp. 242-245
Author(s):  
Jutarut Pornpunyapat ◽  
Wilaiwan Chotigeat ◽  
Pakamas Chetpattananondh
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Concentration ◽  
Renewable Resource ◽  
Reducing Sugar ◽  
Total Sugar ◽  
Bioethanol Production ◽  
Pineapple Peel ◽  
Initial Ph ◽  
Production Characteristics

Bioethanol is widely used as renewable resource due to its safe to produce and environmentally friendly. However, knowledge on ethanol production from pineapple peel juice (Pattawia spp) is far from sufficient. In this work, pineapple peel juice (initial pH at 5) was fermented at various yeast contents (1, 3 and 5% by wt.) and fermentation times (3, 5 and 7 days) in order to investigate ethanol production characteristics. Yeast, Sacchromyces cerevisiae was grown on pineapple peel juice. The squeezed juice contained 11% of total sugar and 5% of reducing sugar. The results indicated that the optimum ethanol production was yeast contents of 5% by wt. and fermentation times of 5 days which gave the ethanol production of 9.08g/l. The ethanol at a higher yeast content also had a higher ethanol concentration.

scholarly journals STUDI PENGARUH pH AWAL MEDIA DAN KONSENTRASI SUBSTRAT PADA PROSES FERMENTASI PRODUKSI BIOETANOL DARI HIDROLISAT TEPUNG BIJI KLUWIH (Actinocarpus communis) DENGAN MENGGUNAKAN Saccharomyces cerevisiae

2018 ◽  
Vol 6 (2) ◽  
pp. 115
Author(s):  
I Gede Yogi Wikrama Yuda ◽  
I Made Mahaputra Wijaya ◽  
Ni Putu Suwariani
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Substrate Concentration ◽  
Fermentation Process ◽  
Total Suspended Solid ◽  
Suspended Solid ◽  
Solid Substrate ◽  
Ethanol Content ◽  
Optimum Ph ◽  
Initial Ph ◽  
Descriptive Method

The aims of this study were to determine the optimum pH of media and substrate concentration on fermentation process that affected the production of bioetanol from kluwih (Actinocarpus communis) seed by Saccharomyces cerevisiae, and to obtain initial pH of media and substrate concentration on fermentation process that can a obtain the highest concentration of bioethanol. The research was designed by 2 factors. The first factor is initial pH of media that consist of three levels: 4, 4.5, and 5. The second factor is substrate concentration that consist of three levels: 2.5 %, 5 %, and 7.5 %. The data was obtained from series of tests and then it analyzed and produced presented by descriptive method. The results showed that the initial pH of media had an effect on ethanol content, and the pH, but not effected on total resulting suspended solid. Substrate concentration had an effect on ethanol content and total suspended solid. Interaction of both treatments had an effect on ethanol content from fermented flour of kluwih seed by used Saccharomyces cerevisiae. The best treatment to produce ethanol from fermented flour of  kluwih seed is by using the initial pH of media 4.5 and substrate concentration amount 7.5 % that yielded the highest ethanol content of 6.13 mL.                                                                                                                                           Key words:  Actinocarpus communis, ethanol, fermentation, the initial pH of media, substrate concentration

Synthesis of (S)-Tert-Butyl 3-Hydroxybutyrate by Asymmetric Reduction of Tert-Butyl Acetoacetate with Saccharomyces cerevisiae B5

2013 ◽  
Vol 704 ◽  
pp. 12-17
Author(s):  
Zhi Min Ou ◽  
Wen Fei Feng ◽  
Li Xu
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Substrate Concentration ◽  
Asymmetric Reduction ◽  
Optical Purity ◽  
Lower Amount ◽  
High Optical Purity ◽  
Initial Substrate ◽  
Initial Ph ◽  
H 30 ◽  
Initial Substrate Concentration

S)-tert-butyl 3-hydroxybutyrate was synthesized by asymmetric reduction of tert-butyl acetoacetate with Saccharomyces cerevisiae B5 as catalyst. The enantiometric excess of (S)-tert-butyl 3-hydroxybutyrate increased with addition of more amount of substrate. High optical purity of product can be obtained when 6 g/L chloroform was used as inhibitor. The optimum reduction time, temperature, and initial pH of reaction mixture were 60 h, 30 °C, and 6.2. Addition of more biomass and lower amount of substrate helped to get high conversion. Conversion and enantiometric excess of product reached 100% when initial substrate concentration and biomass were 2.0 g/L and 140 g/L with 6 g/L chloroform as inhibitor.

Optimization of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Cotton Stalk

2013 ◽  
Vol 724-725 ◽  
pp. 391-398
Author(s):  
Qin Zhang ◽  
Yan Bin Li ◽  
Zhan Wen Liu ◽  
Yun Feng Pu ◽  
Li Ming Xia
Keyword(s):  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Ph Value ◽  
Raw Material ◽  
Cotton Stalk ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Experimental Yield ◽  
Simultaneous Saccharification

Steam-exploded cotton stalk was used as raw material in ethanol production through simultaneous saccharification and fermentation by Penicillium Q59 and Saccharomyces cerevisiae P1. The fermentative conditions were firstly examined by single factor experiments to determine the central point in Box-Behnken design, which was explored with expectation to get optimized fermentative conditions for enhancement of ethanol production. The results of optimized fermentative conditions were determined as follows: fermentation time was 10.5 days, bran added percent was 15%, initial pH value was 5.5. Under the optimal conditions, the experimental yield of ethanol was 99.85 ± 4.21 g·kg-1SECS (steam-exploded cotton stalk), which was close to the theoretical predicting value, it showed the model was feasible. The research results will provide technical reference for further exploitation of cotton stalk.

Study on the Fermentation Conditions of β-Glucosidase from Aspergillus niger

2014 ◽  
Vol 1033-1034 ◽  
pp. 133-137
Author(s):  
Shao Hua Luo ◽  
Chao Zhang ◽  
Jin Ling Guo ◽  
Hua Shun Yu ◽  
Juan Yao ◽  
...  
Keyword(s):  
Aspergillus Niger ◽  
Rotational Speed ◽  
Hot Spot ◽  
Scaling Up ◽  
Biomass Energy ◽  
Optimum Conditions ◽  
Fermentation Conditions ◽  
Seed Age ◽  
Initial Ph ◽  
Key Enzyme

Biomass energy is environmentally friendly, renewable, and a rich stock.The research and development of biomass energy have become a hot spot. The β-glucosidase is the key enzyme in the utilization of biomass. The fermentation conditions for β-glucosidase production fromAspergillus nigerwere optimized. The optimum conditions were as follows: the seed age 48h, the inoculation amount 10%, the medium volume 20mL / 250mL, the initial pH 5.0, the rotational speed 200 r/ min, Under these conditions, the activity of β-glucosidase can research 13.92 U ∕ mL after 168h, increasing 92.8% than before. This experiment can provide the basis for the next step of liquid fermentation for the scaling up production of β-glucosidase.

scholarly journals Enhanced ethanol production from sugarcane molasses by industrially engineered Saccharomyces cerevisiae via replacement of the PHO4 gene

RSC Advances ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 2267-2276 ◽  
Author(s):  
Renzhi Wu ◽  
Dong Chen ◽  
Shuwei Cao ◽  
Zhilong Lu ◽  
Jun Huang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Ethanol Production ◽  
Ethanol Fermentation ◽  
Ethanol Yield ◽  
Regulatory Gene ◽  
Sugarcane Molasses ◽  
Fermentation Time ◽  
Fast Growing ◽  
Novel Strategy ◽  
Engineered Saccharomyces Cerevisiae

Replacement of a novel candidate ethanol fermentation-associated regulatory gene, PHO4, from a fast-growing strain through a novel strategy (SHPERM-bCGHR), is hypothesised to shorten fermentation time and enhance ethanol yield from sugarcane molasses.

scholarly journals Determination of the Optimum Conditions for Ethanol Production from Whey by Utilization the Local Isolate Kluyveromyces sp.

2009 ◽  
Vol 3 (1) ◽  
pp. 63-71
Author(s):  
Akram T. Alrawi ◽  
Raad A. Alkusairy ◽  
Abdulmajed AL-Samarraie
Keyword(s):  
Ethanol Production ◽  
Cheese Whey ◽  
Ethanol Yield ◽  
Fermentation Medium ◽  
Optimum Conditions ◽  
Fermentation Efficiency ◽  
Date Syrup ◽  
Initial Ph ◽  
Series Of Experiments

A series of experiments were conducted to explore the optimum conditions for ethanol production from cheese whey. The optimum conditions were: incubation period, 8 days, fermentation temperature, 30◦c, Size of inoculum, 1.15 x 106 C.F.U/ml, initial pH of medium, 5.0. The optimum composition of the fermentation medium were : Whey containing 9.3% lactose and fortified with ( NH4)SO4, 0.015 % , Yeast Extract, 0.01% , K2HPO4, 0.015% and it was found that the addition of date Syrup at a level of 0.5% noticeably increased ethanol yield compared with the medium which contain no date Syrup. By collective utilization of all the optimum conditions mentioned above, the following results were obtained, metabolized lactose 70.9%, ethanol production 4.48% and fermentation efficiency 132.36%.

scholarly journals Ethanol Production from Gmelina arborea Wood Wastes by Saccharomyces cerevisiae using Submerged Fermentation

2021 ◽  
Vol 37 (2) ◽  
pp. 144-151
Author(s):  
M.R Adedayo ◽  
A.E Ajiboye ◽  
O.A Yahaya
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Specific Gravity ◽  
Ethanol Production ◽  
Submerged Fermentation ◽  
Ethanol Concentration ◽  
Sugar Content ◽  
Reducing Sugar ◽  
Gmelina Arborea ◽  
Fermentation Time ◽  
Standard Curve

Lignocellulose wastes are the most abundant residues on the surface of the earth. This project studies the possibility of ethanol production from a forestry waste. Wood wastes from Gmelina arborea were treated with dillute sulfuric acid to break down the lignin component. Fermentation for ethanol production was done using baker’s yeast (Saccharomyces cerevisiae ATCC 204508/S288c) for 120 hours using submerged fermentation, and the pH, reducing sugar, specific gravity and lignin content were determined using standard techniques. Ethanol concentration and yield were measured via vinometer and ethanol standard curve techniques. From the results, the highest pH was obtained at 72 hours of the fermentation period. The reducing sugar content and specific gravity decreased over the fermentation time . The acid-pretreated wood wastes gave a maximum ethanol concentration of 3.84 % and a yield of 7.60 ml/g as measured from the vinometer and ethanol standard curve methods at 72 and 96 hours of fermentation, respectively. About 13.6% v/v of ethanol was recovered from the distillation process employed to separate the components of the product generated after fermentation. The observations in this research reveal the possibility of producing ethanol from G. arborea wood wastes and under optimized culture conditions. This could serve as an alternate means of biofuel generation and hence value addition to the wastes. Keywords: Gmelina arborea, Saccharomyces cerevisiae, Ethanol, Submerged fermentation

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