scholarly journals Fluorescent N-doped carbon dots from bacterial cellulose for highly sensitive bacterial detection

BioResources ◽  
2019 ◽  
Vol 15 (1) ◽  
pp. 78-88
Author(s):  
Shunli Li ◽  
Xin Zhao ◽  
Yujie Zhang ◽  
Honglei Chen ◽  
Yu Liu

Carbon dots have good dispersion capability, strong visible fluorescence, low toxicity, and photo-induced accepting and donating abilities. Carbon dots were obtained from biomass bacterial cellulose (BC) via one-step hydrothermal carbonization. Effects of hydrothermal time and temperature on the microstructure, fluorescence, and excitation wavelength dependent photoluminescence (PL) behavior were explored for the prepared carbon dots. The results showed that the carbon dots obtained directly from the BC (C dots) had small particle sizes (2.0 to 3.0 nm) and green luminescence behavior. Conversely, the N-doped carbon dots (N-C dots) exhibited more uniform and smaller particle sizes (approximately 1.0 nm), strong blue luminescence, acceptable fluorescence lifetime, and good stability in a wide range of pH values (2.0 to 10.0). Thus, carbon dots could serve as a fluorescent material used in high performance optical cellular imaging and highly sensitive bacterial detection.

2020 ◽  
Vol 25 (46) ◽  
pp. 4848-4860 ◽  
Author(s):  
Anisha Anand ◽  
Gopinathan Manavalan ◽  
Ranju Prasad Mandal ◽  
Huan-Tsung Chang ◽  
Yi-Ru Chiou ◽  
...  

: The prevention and treatment of various infections caused by microbes through antibiotics are becoming less effective due to antimicrobial resistance. Researches are focused on antimicrobial nanomaterials to inhibit bacterial growth and destroy the cells, to replace conventional antibiotics. Recently, carbon dots (C-Dots) become attractive candidates for a wide range of applications, including the detection and treatment of pathogens. In addition to low toxicity, ease of synthesis and functionalization, and high biocompatibility, C-Dots show excellent optical properties such as multi-emission, high brightness, and photostability. C-Dots have shown great potential in various fields, such as biosensing, nanomedicine, photo-catalysis, and bioimaging. This review focuses on the origin and synthesis of various C-Dots with special emphasis on bacterial detection, the antibacterial effect of CDots, and their mechanism.


The Analyst ◽  
2021 ◽  
Vol 146 (20) ◽  
pp. 6297-6305
Author(s):  
Qinglan Miao ◽  
Ji Qi ◽  
Yuanyuan Li ◽  
Xinxia Fan ◽  
Dongmei Deng ◽  
...  

A novel paper-based chip that anchored zinc-doped carbon dots was constructed for sensitive and stable fluorescent detection of Cu2+. Zn doping increased the active sites for simplifying the modification of carbon dots.


2021 ◽  
Author(s):  
Le Wang ◽  
Wenjie Zhou ◽  
Dan Yang ◽  
Zhe Hu ◽  
Shiliang Mei ◽  
...  

Correction for ‘Gadolinium-doped carbon dots with high-performance in dual-modal molecular imaging’ by Le Wang et al., Anal. Methods, 2021, 13, 2442–2449, DOI: 10.1039/d1ay00270h.


2018 ◽  
Vol 63 ◽  
pp. 237-243 ◽  
Author(s):  
Xiao Gong ◽  
Wenwen Ma ◽  
Yunxia Li ◽  
Lingqi Zhong ◽  
Wenjing Li ◽  
...  

2019 ◽  
Vol 102 (6) ◽  
pp. 1666-1672
Author(s):  
Manisha Dhanshetty ◽  
Kaushik Banerjee

Background: Mycotoxins such as aflatoxins (AFs) and ochratoxin A (OTA) can pose severe health hazards because of their toxicity. Given a wide range of food matrices susceptible to fungal infections and possible cooccurrence of mycotoxins at different concentrations, validated multimycotoxin and multimatrix methods are strongly warranted. Objective: The aim of this research was to develop a simple and fast ultra-high performance LC (UHPLC) fluorescence detection (FLD)–based method to simultaneously determine AFs (B1, G1, B2, and G2) and OTA and, furthermore, to carry out single-laboratory validation in a range of cereals and processed product matrices. Methods: The sample preparation involved homogenization and extraction with methanol–water (80 + 20). For cleanup, an aliquot (3 mL) was diluted with phosphate-buffered saline, loaded on an immunoaffinity column (AFLAOCHRA PREP®), and eluted with methanol (1 mL). The cleaned extract was diluted with 0.2% acetic acid (at a 1:1 ratio) before injection into an ultra-high performance liquid chromatograph. To perform simultaneous analysis of AFs and OTA, the FLD program was developed by switching the excitation wavelength in a single chromatographic run. Results: The method provided LOQs of 0.25 and 1 ng/g for AFs and OTA, respectively, without involving any derivatization. In rice, the recoveries of AFs ranged from 84 to 106%, whereas OTA had a recovery above 72%, with the repeatability relative SDs <12% for both analytes. The method was successfully applied to a range of naturally contaminated market samples. Conclusions: The method is suitable for regulatory testing because of its significant time and cost effectiveness and sensitivity in compliance with the regulatory maximum levels. Highlights: The study achieves high-throughput analysis of AFs and OTA in raw and processed cereals using simultaneous extraction, cleanup, and UHPLC-FLD. Method sensitivity complies with the regulatory maximum levels. Single-laboratory validation results meet analytical QC requirements.


2019 ◽  
Vol 11 (20) ◽  
pp. 2650-2657 ◽  
Author(s):  
Yifang Gao ◽  
Huilin Zhang ◽  
Shaomin Shuang ◽  
Hui Han ◽  
Chuan Dong

Novel nitrogen co-doped carbon dots (NCDs) were synthesized as a fluorescent “on–off–on” switch for the highly sensitive and selective sensing of Cu2+ and glutathione (GSH) by a straightforward pyrolysis route.


RSC Advances ◽  
2016 ◽  
Vol 6 (18) ◽  
pp. 15192-15200 ◽  
Author(s):  
Huan Yuan ◽  
Jie Yu ◽  
Suling Feng ◽  
Yijun Gong

Highly photoluminescent pH-independent nitrogen-doped carbon dots prepared from maleic acid and ethylenediamine exhibit highly sensitive and selective sensing of p-nitrophenol.


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