ANATOMO-MORPHOLOGICAL STRUCTURE AND CHLOROPHYLL CONTENT IN APPLE LEAVES UNDER APPLICATION OF FERTILIZERS AND BIOACTIVE SUBSTANCES

2013 ◽  
pp. 80-84
Author(s):  
N.N. Sergeeva ◽  
◽  
N.I. Nen'ko ◽  
G.K. Kiseleva ◽  
◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Morphological Structure ◽  
Bioactive Substances ◽  
Apple Leaves

scholarly journals Ethnobotanical Study, Anatomical Study and Phytochemical Screening of Aristolochia longa L.

2020 ◽  
Vol 10 (4) ◽  
pp. 112-124
Author(s):  
N. Merouani ◽  
H. Boukhebti ◽  
R. Belhattab
Keyword(s):  
Local Population ◽  
Morphological Structure ◽  
Anatomical Study ◽  
New Drugs ◽  
Ethnobotanical Study ◽  
Phytochemical Screening ◽  
Bioactive Substances ◽  
Traditional Use ◽  
Treatment Of Wounds ◽  
Promising Source

Aristolochia longa L. (Aristolochiaceae) is used in Algerian traditional medicine. The ethnobotanical study conducted in the region of Setif (East Algeria) has as an objective of evaluating the potential of the region of Aristolochia longa L. used in the treatment of different diseases. A questionnaire was used which consisted of the diseases treated by this plant, the part of the plant used in the treatment and how to use this plant. The survey targeted 100 people from the local population. We also evaluated the phytochemical composition of the aerial parts (stems and leaves), fruits and tubers. Our results showed that A. longa is widely used to treat several ailments such as cancer (43%), diabetes (17%), and treatment of wounds in cattle (12%), and intestinal and stomach diseases (9 and 7%), the most used part is tubers by 70%.Crushed tubers are commonly mixed with honey(44%), milk(24%), water (8%). Results of the phytochemical screening revealed that A. longa contained various bioactive compounds, including polyphenols, flavonoids, saponins, terpenoids, and Alkaloids. In addition, there is very little information concerning the anatomical and morphological structure of this species of Setif region (Algeria), a preliminary study on anatomy of this plant is therefore reported in this paper. These preliminary results could be used to justify the traditional use of this plant and their bioactive substances could be exploited for therapeutic purposes such as antioxidant, antimicrobial and anti-inflammatory, and may be considered as a promising source of new drugs for treating cancer. Keywords: Aristolochia longa L., ethnobotany Setif(Algeria), anatomical study of plant, phytochemical screening.

scholarly journals Optimization of chlorophyll extraction solvent of bulung sangu (Gracilaria sp.) seaweed

2021 ◽  
Vol 913 (1) ◽  
pp. 012073
Author(s):  
M M V Sasadara ◽  
N M D M W Nayaka ◽  
P E S K Yuda ◽  
N L K A A Dewi ◽  
E Cahyaningsih ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Bioactive Substances ◽  
Chlorophyll Contents ◽  
Extraction Solvent ◽  
Red Macroalgae ◽  
Pigment Extraction ◽  
Chlorophyll D

Abstract Algae are a photosynthetic organism, affordable and naturally rich in nutrients and a valuable source of bioactive substances such as natural pigments. Bulung sangu (Gracilaria sp.) is red macroalgae that wildly grows and distributes in Bali. The aim of this work was to optimize the solvent to extract the chlorophyll content of Bulung sangu. The pigment extraction was carried out using different solvents (100% methanol, 100% ethanol, and 90% acetone). The chlorophyll contents including chlorophyll a,b,c,d and total chlorophyll were measured using spectrophotometry UV-VIS and expressed in µg/g of algae. The results showed that chlorophyll c could not be extracted using all used solvent, while chlorophyll b can only be extracted using acetone. Acetone produced the highest concentration of chlorophyll a (717.52 ± 9.71 µg/g), chlorophyll b (7.23 ± 0.24 µg/g), chlorophyll d (21.93 ± 1.07 µg/g), and chlorophyll total (746.67 ± 8.99 µg/g) compared to other solvent, that were significantly different (p<0.05). The second solvent to produce the highest concentration of chlorophyll a, d, and total chlorophyll was methanol which produced 578.77 ± 9.74 µg/g, 5.50 ± 0.12 µg/g and 584.27 ± 9.62 µg/g of chlorophyll content, respectively, followed by ethanol which produced 520.98 ± 2.52 µg/g of chlorophyll a, 3.56 ± 0.25 µg/g for chlorophyll d, and 524.54 ± 2.30 µg/g for total chlorophyll. Acetone is considered the most effective solvent to extract the chlorophyll content of Bulung sangu.

Estimation of Chlorophyll Content in Apple Leaves Based on Imaging Spectroscopy

2019 ◽  
Vol 86 (3) ◽  
pp. 457-464
Author(s):  
Ruiyang Yu ◽  
Xicun Zhu ◽  
Shujing Cao ◽  
Jingling Xiong ◽  
Xin Wen ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Imaging Spectroscopy ◽  
Apple Leaves

scholarly journals Cytological, genetic, and proteomic analysis of a sesame (Sesamum indicum L.) mutant Siyl-1 with yellow–green leaf color

2019 ◽  
Vol 42 (1) ◽  
pp. 25-39 ◽  
Author(s):  
Tong-Mei Gao ◽  
Shuang-Ling Wei ◽  
Jing Chen ◽  
Yin Wu ◽  
Feng Li ◽  
...  
Keyword(s):  
Genetic Analysis ◽  
Chlorophyll Content ◽  
Chloroplast Development ◽  
Regulatory Mechanism ◽  
Morphological Structure ◽  
Green Leaf ◽  
Yellow Leaf ◽  
Leaf Color ◽  
Wild Genotype ◽  
Color Mutation

Abstract Background Both photosynthetic pigments and chloroplasts in plant leaf cells play an important role in deciding on the photosynthetic capacity and efficiency in plants. Systematical investigating the regulatory mechanism of chloroplast development and chlorophyll (Chl) content variation is necessary for clarifying the photosynthesis mechanism for crops. Objective This study aims to explore the critical regulatory mechanism of leaf color mutation in a yellow–green leaf sesame mutant Siyl-1. Methods We performed the genetic analysis of the yellow-green leaf color mutation using the F2 population of the mutant Siyl-1. We compared the morphological structure of the chloroplasts, chlorophyll content of the three genotypes of the mutant F2 progeny. We performed the two-dimensional gel electrophoresis (2-DE) and compared the protein expression variation between the mutant progeny and the wild type. Results Genetic analysis indicated that there were 3 phenotypes of the F2 population of the mutant Siyl-1, i.e., YY type with light-yellow leaf color (lethal); Yy type with yellow-green leaf color, and yy type with normal green leaf color. The yellow-green mutation was controlled by an incompletely dominant nuclear gene, Siyl-1. Compared with the wild genotype, the chloroplast number and the morphological structure in YY and Yy mutant lines varied evidently. The chlorophyll content also significantly decreased (P < 0.05). The 2-DE comparison showed that there were 98 differentially expressed proteins (DEPs) among YY, Yy, and yy lines. All the 98 DEPs were classified into 5 functional groups. Of which 82.7% DEPs proteins belonged to the photosynthesis and energy metabolism group. Conclusion The results revealed the genetic character of yellow-green leaf color mutant Siyl-1. 98 DEPs were found in YY and Yy mutant compared with the wild genotype. The regulation pathway related with the yellow leaf trait mutation in sesame was analyzed for the first time. The findings supplied the basic theoretical and gene basis for leaf color and chloroplast development mechanism in sesame.

scholarly journals Estimating Chlorophyll Content of Apple Leaves Based on Different Scales in Differential Window

2015 ◽  
Vol 06 (09) ◽  
pp. 1106-1114
Author(s):  
Zhaoying Han ◽  
Xicun Zhu ◽  
Zhuoyuan Wang ◽  
Gengxing Zhao ◽  
Ling Wang
Keyword(s):  
Chlorophyll Content ◽  
Apple Leaves

scholarly journals Visualization of Chlorophyll Content Distribution in Apple Leaves Based on Hyperspectral Imaging Technology

2019 ◽  
Vol 10 (06) ◽  
pp. 783-795
Author(s):  
Xin Wen ◽  
Xicun Zhu ◽  
Ruiyang Yu ◽  
Jingling Xiong ◽  
Dongsheng Gao ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Hyperspectral Imaging ◽  
Content Distribution ◽  
Imaging Technology ◽  
Apple Leaves

High-resolution topographic SEM and radiation damage: The rationale for using low beam voltage

1992 ◽  
Vol 50 (2) ◽  
pp. 1278-1279
Author(s):  
James Pawley ◽  
David Joy
Keyword(s):  
High Resolution ◽  
Imaging Techniques ◽  
Morphological Structure ◽  
Radiation Sensitivity ◽  
Beam Specimen ◽  
Measured Signal ◽  
Practical Consideration ◽  
Interaction Volume ◽  
Impact Area ◽  
Signal Contrast

The scanning electron microscope (SEM) builds up an image by sampling contiguous sub-volumes near the surface of the specimen. A fine electron beam selectively excites each sub-volume and then the intensity of some resulting signal is measured and then plotted as a corresponding intensity in an image. The spatial resolution of such an image is limited by at least three factors. Two of these determine the size of the interaction volume: the size of the electron probe and the extent to which detectable signal is excited from locations remote from the beam impact area. A third limitation emerges from the fact that the probing beam is composed of a number of discrete particles and therefore that the accuracy with which any detectable signal can be measured is limited by Poisson statistics applied to this number (or to the number of events actually detected if this is smaller). As in all imaging techniques, the limiting signal contrast required to recognize a morphological structure is constrained by this statistical consideration. The only way to overcome this limit is to increase either the contrast of the measured signal or the number of beam/specimen interactions detected. Unfortunately, these interactions deposit ionizing radiation that may damage the very structure under investigation. As a result, any practical consideration of the high resolution performance of the SEM must consider not only the size of the interaction volume but also the contrast available from the signal producing the image and the radiation sensitivity of the specimen.

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