scholarly journals Optimization of chlorophyll extraction solvent of bulung sangu (Gracilaria sp.) seaweed

2021 ◽  
Vol 913 (1) ◽  
pp. 012073
Author(s):  
M M V Sasadara ◽  
N M D M W Nayaka ◽  
P E S K Yuda ◽  
N L K A A Dewi ◽  
E Cahyaningsih ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Bioactive Substances ◽  
Chlorophyll Contents ◽  
Extraction Solvent ◽  
Red Macroalgae ◽  
Pigment Extraction ◽  
Chlorophyll D

Abstract Algae are a photosynthetic organism, affordable and naturally rich in nutrients and a valuable source of bioactive substances such as natural pigments. Bulung sangu (Gracilaria sp.) is red macroalgae that wildly grows and distributes in Bali. The aim of this work was to optimize the solvent to extract the chlorophyll content of Bulung sangu. The pigment extraction was carried out using different solvents (100% methanol, 100% ethanol, and 90% acetone). The chlorophyll contents including chlorophyll a,b,c,d and total chlorophyll were measured using spectrophotometry UV-VIS and expressed in µg/g of algae. The results showed that chlorophyll c could not be extracted using all used solvent, while chlorophyll b can only be extracted using acetone. Acetone produced the highest concentration of chlorophyll a (717.52 ± 9.71 µg/g), chlorophyll b (7.23 ± 0.24 µg/g), chlorophyll d (21.93 ± 1.07 µg/g), and chlorophyll total (746.67 ± 8.99 µg/g) compared to other solvent, that were significantly different (p<0.05). The second solvent to produce the highest concentration of chlorophyll a, d, and total chlorophyll was methanol which produced 578.77 ± 9.74 µg/g, 5.50 ± 0.12 µg/g and 584.27 ± 9.62 µg/g of chlorophyll content, respectively, followed by ethanol which produced 520.98 ± 2.52 µg/g of chlorophyll a, 3.56 ± 0.25 µg/g for chlorophyll d, and 524.54 ± 2.30 µg/g for total chlorophyll. Acetone is considered the most effective solvent to extract the chlorophyll content of Bulung sangu.

scholarly journals Pemanfaatan Pupuk Organik Kulit Pisang Dan Azolla Terhadap Pertumbuhan Kangkung Darat Dengan Sistem Akuaponik

2019 ◽  
Vol 12 (01) ◽  
pp. 1-10
Author(s):  
Maria Sastriana ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Organic Fertilizer ◽  
Control Treatment ◽  
Banana Peel ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Azolla Pinnata ◽  
Randomized Design ◽  
Weight Test

The aim of this study was to compare the organic fertilizer of azolla pinnata and banana peel and a combination of azolla pinnata and banana peel with the same concentration of 800 ppm to the weight of harvest and the chlorophyll content of ground spinach (Ipomoea reptans Poir.). The experiment was carried out experimentally using a completely randomized design with control treatment, 800 ppm azolla pinnata, 800 ppm banana skin and a combination of azolla pinnata and banana peels 800 ppm which were repeated 6 times each. The application was carried out by pouring 800 ppm of azolla pinnata, 800 ppm of banana peel and a combination of azolla pinnata and 800 ppm banana peel on each gutter. The results showed that azolla pinnata and banana peel nutrition had a significant effect (P <0.05) on harvest weight and chlorophyll content of ground spinach plants (Ipomoea reptans Poir.). Nutrition azolla pinnata and banana peel with a concentration of 800 ppm can increase the weight of the land spinach (Ipomoea reptans Poir.) Which has the highest average. In testing the chlorophyll content, the results of chlorophyll a content, chlorophyll b content and the highest total chlorophyll content was found in azolla pinnata 800 ppm nutrition. The results of this study concluded that 800 ppm azolla pinnata, 800 ppm banana peel and 800 ppm combination of azolla pinnata and banana peel in the growth of ground spinach plants (Ipomoea reptans Poir.) Were effective to increase harvest weight and increase chlorophyll content of land spinach (Ipomoea reptans Poir.) Namely chlorophyll a, chlorophyll b and total chlorophyll of land spinach plants (Ipomoea reptans Poir.).   Keywords: ground kale, azolla pinnata, banana peel, harvest weight, test of chlorophyll content, chlorophyll a, chlorophyll b, total chlorophyll.

scholarly journals Effect of nicosulfuron on chlorophyll content of marandu-grass cultivated in crop-forest-livestock integration systems

2020 ◽  
pp. 1876-1882
Author(s):  
Evander Alves Ferreira ◽  
Marcia Vitória Santos ◽  
Leandro Diego da Silva ◽  
Priscila Júnia Rodrigues da Cruz ◽  
Raul Ribeiro Silveira ◽  
...  
Keyword(s):  
Growth Rate ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Dry Matter ◽  
Agroforestry Systems ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Integration System ◽  
Total Chlorophyll Content ◽  
Urochloa Brizantha

The aim of this work was to study the effect of sub-doses of herbicide nicosulfuron on the levels of chlorophyll and growth of marandu-grass in an forest-livestock integration system. The treatments consisted of eucalyptus consortium with sorghum (BRS 655 hybrid) and forage Urochloa brizantha cv. Marandu (marandu-grass) at 12×2 and 12×3 m spacings in agroforestry systems, in addition to sorghum + marandu-grass and marandu-grass in monoculture. The doses of nicosulfuron applied at the marandu-grass were 0, 15 and 30 g ha-1. At 30 and 120 days after the application of nicosulfuron, chlorophyll a and chlorophyll b were measured usinf a portable chlorophyll meter, with six measurements per plant. Afterwards, the total chlorophyll content and chlorophyll a/b were calculated. We also measured the mass of the total dry matter of marandu-grass and growth rate of the culture. Marandu-grass plants cultivated in monoculture showed higher quantities of chlorophyll and the highest growth rates. The levels of chlorophyll a, b and chlorophyll a/b and the growth rate of marandu grass were similar between systems integrated with eucalyptus (12x2 and 12x3 m). The increase of herbicide dose negatively influenced on chlorophyll content in marandu-grass in all treatments evaluated. The use of nicosulfuron in doses of 25 and 50 g L-1 reduced the growth rate of marandu grass only when grown in monoculture

scholarly journals Synthesis, Characterization and Herbicidal Activity of Amide Derivatives of Glyphosate

2018 ◽  
Vol 34 (5) ◽  
pp. 2378-2383 ◽  
Author(s):  
Jatinder Pal Kaur Gill ◽  
Nidhi Sethi ◽  
Anand Mohan
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Structure Analysis ◽  
Herbicidal Activity ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Amide Derivatives ◽  
The Common ◽  
Derivatives Of

The present work includes synthesis of a series of amide derivatives of glyphosate and their characterization. The structure analysis of these new derivatives was done with the help of FTIR and 1H NMR, Further, their herbicidal activity was analyzed on one of the common weeds (Parthenium hysterophorus). Under the influence of amide derivatives of glyphosate it was found that the chlorophyll content (Chlorophyll a, Chlorophyll b and total Chlorophyll content) of the weed was found to lessen than the control. Moreover, these synthesized derivatives are less polar as compared to the parent glyphosate molecule thereby can emphatically reduce the problem of their leaching into the groundwater.

scholarly journals Effect of IBA, NAA and their Combination on Rooting and Biochemical Parameters of Stem Cuttings in Barbados Cherry

2021 ◽  
Vol 8 (5) ◽  
pp. 147-150
Author(s):  
Alam Khan Samim ◽  
B. S. Shivakumar ◽  
Ganapathi M.
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Growth Regulators ◽  
Biochemical Parameters ◽  
Root Initiation ◽  
Stem Cuttings ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Fresh Weight ◽  
Total Chlorophyll Content

An investigation was to assess the influence of growth regulators IBA, NAA and their combination on rooting and biochemical parameters of stem cuttings in Barbados cherry. It was observed that, the least number of days (21.33) taken for root initiation, maximum percentage of rooting (80.00) was observed in cutting treated with IBA 5000 ppm followed by IBA 4500 ppm over other treatments. Significantly highest Chlorophyll-a (1.26), Chlorophyll –b (0.55) and total chlorophyll content (1.82 mg/g of fresh weight) of leaves and C : N ratio (8.74) was observed in cuttings treated with IBA 5000 ppm followed by IBA 4500 ppm over all other treatments.

Foliar application of urea and potassium chloride minimizes terminal moisture stress in lentil (Lens culinaris L.) crop

2019 ◽  
Author(s):  
Gulab Singh Yadav ◽  
A Gangarani Devi ◽  
Anup Das ◽  
Basant Kandpal ◽  
Subhash Babu ◽  
...  
Keyword(s):  
Potassium Chloride ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Lens Culinaris ◽  
Foliar Application ◽  
Moisture Stress ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Formation Stage

Soil moisture stress in lentil (Lens culinaris L.) cultivated in rice (Oryza sativa L.) fallows of Tripura (50 m above msl), India, is one of the issues related to low productivity. Effects of foliar feeding of lentil with urea and potassium chloride KCI @ 2% each, singly or in combination either at 50% flowering, 50% podding or both at 50% flowering + 50% pod formation stages under moisture stress condition were investigated. The key physiological parameters viz. chlorophyll a, chlorophyll b, total chlorophyll content, leaf relative water content (LRWC), excised leaf water loss (ELWL) and yield attributes of lentil were measured. There were significant plessthan0.05) increases in chlorophyll a, chlorophyll b and total chlorophyll content at 50% flowering and 50% pod formation stage in plants treated either with 2% KCl alone or in combination with 2% urea as compared to those under control and 2% urea alone. Further, LRWC and ELWL were the highest (83%) and lowest (0.7%) under combined application of two agro chemicals, respectively. Significant (plessthan 0.05) increases in plant height, number of branches per plant, numbers of pods per plant and biomass as well as seed yield were also observed with combined foliar application of agrochemicals in comparison to those under control. The highest LRWC and lowest ELWL were recorded in case of foliar spray at 50% flowering + 50% pod formation as compared to either 50% flowering or 50% pod formation stage alone. Foliar application of both agro-chemicals mitigates the terminal moisture stresses especially in underutilized rice-fallow lands for successful cultivation of rabi crops like lentil.

scholarly journals Pengaruh Perbandingan Bahan Pelarut dan Lama Ekstraksi terhadap Karakteristik Ekstrak Pewarna Daun Pandan Wangi (Pandanus amaryllifolius Roxb.)

2019 ◽  
Vol 7 (4) ◽  
pp. 571
Author(s):  
I Made Dwipayana ◽  
Ni Made Wartini ◽  
Luh Putu Wrasiati
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Block Design ◽  
Extraction Time ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Brightness Level ◽  
Extract Yield ◽  
Pandanus Amaryllifolius

Dyes from fragrant pandan leaves contain chlorophyll obtained through the extraction process. The purpose of this study were to determine the effect of the ratio of ingredients to solvents and the length of extraction on the characteristics of natural coloring extracts of fragrant pandan leaves and determine the best treatment from the comparison of ingredients with solvents and extraction time to produce natural coloring extracts of fragrant pandan leaves. This study used a 2 factor factorial randomized block design. The first factor is the comparison of materials with solvents consisting of 3 levels, namely 1:13, 1:15, and 1:17. The results showed that the comparative treatment of the material with the solvent and the extraction time greatly influenced the extract yield, chlorophyll a levels, chlorophyll b levels, total chlorophyll levels, brightness level L *, redness level a *, yellowish level b *, interactions greatly influenced the levels chlorophyll a, chlorophyll b level, total chlorophyll content, redness level (a *), yellowish level (b *) but have no effect on extract yield and brightness level (L *). Comparison of material treatment with solvent 1:15 and extraction time for 36 hours, is the best treatment to produce pandanus fragrant leaf extract coloring with extract yield characteristics of 10.32%, chlorophyll a levels of 3402.04 ppm, chlorophyll b levels of 612.23 ppm, total chlorophyll content of 4013.42 ppm, brightness level (L *) 35.27, redness level (a *) 17.15, yellowish level (b *) 30.43. Keywords: Pandanus amaryllifolius Roxb., chlorophyll, nature color, extraction.

scholarly journals UJI BANDING BERBAGAI MEDIA TANAM TERHADAP PERTUMBUHAN SELADA MERAH (Lactuca sativa var. crispa L) DENGAN MEDIA TANAM HIDROPONIK SISTEM NFT (Nutrient Film Technique)

2018 ◽  
Vol 11 (01) ◽  
pp. 23-32
Author(s):  
G. F. Yanti ◽  
Ngadiani Ngadiani
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Lactuca Sativa ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Lettuce Plant ◽  
Randomized Design ◽  
Nutrient Film Technique ◽  
Weight Test ◽  
Wet Weight

A study has been conducted to compare some varieties of planting media on red lettuce (Lactuca sativa var crispa L), the variables observed in this study are wet weight and chlorophyll content. This research was conducted by using complete randomized design (RAL) consisting of 5 variants of planting medium (Rockwool (as control), cocopeat, fuel husk, cotton and sponge) with 4 replications. The results showed that rockwool planting media had significant effect (P <0,05) on wet weight of red lettuce plant (Lactuca sativa var. Crispa L). Then, on the results of chlorophyll content test, it was found that the variation of burning husk medium media on red lettuce plant had significant effect (P <0,05) to chlorophyll a and total chlorophyll, while in chlorophyll b the result obtained was not significantly different (P> 0, 05). The results of this study indicate that the treatment of rockwool planting media in red lettuce plant growth is effective to increase wet weight and the treatment of burning husk medium media can increase the chlorophyll content of red lettuce plants namely chlorophyll a and total chlorophyll red lettuce plants (Lactuca sativa var. Crispa L.).   Keywords: red lettuce, wet weight, test of chlorophyll content a, chlorophyll b, total chlorophyll

scholarly journals Phytochemical screening, antioxidant and tyrosinase inhibitory studies of methanol leaf extracts of two tomato varieties

2020 ◽  
Author(s):  
Oluwasegun Victor Omotoyinbo ◽  
Emmanuel Olumide Awojulu ◽  
David Morakinyo Sanni
Keyword(s):  
Lycopersicon Esculentum ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Competitive Inhibition ◽  
Antioxidant Properties ◽  
Total Phenolic ◽  
Chlorophyll B ◽  
Leaf Extracts ◽  
Chlorophyll Contents ◽  
Ic50 Value

This study evaluated the phytochemical compositions, antioxidant properties, chlorophyll content and anti-tyrosinase activity of methanol leaf extracts of two tomato varieties, Lycopersicon esculentum (var. Eva F1) and Lycopersicon esculentum Mill (var. Hausa). The dried pulverized of the plant’s leaves were extracted by decoction and mild agitation. Phytochemicals such as flavonoids, tannin, glycoside, saponin, terpenoid and anthraquinone were present in the extracts of both varieties examined, while alkaloid and phlobatannin were confirmed absent in the extracts. The presence of steroid was observed in var. Eva F1 but absent in var. Hausa. Total phenolic content (TPC) and total flavonoid content (TFC) of var. Eva F1 were 505.9 ± 2.61 mg GAE/ge, and 35.5 ± 1.64 mg RE/ge, while var. Hausa recorded a TPC and TFC value of 344.3 ± 2.01 and 7.8 ± 0.15 mg RE/ge respectively. The chlorophyll content of the extracts were 6.6 ± 0.02 mg/ge (chlorophyll a), 5.7 ± 0.05 mg/ge (chlorophyll b) and 12.6 ± 0.14 mg/ge (total chlorophyll content) for Eva F1 variety, while the chlorophyll contents for var. Hausa were 7.6 ± 0.32 mg.ge (chlorophyll a), 5.6 ± 0.06 mg/ge (chlorophyll b) and 13.7 ± 0.14 mg/ge. Eva F1 and Hausa showed percentage inhibition of 76.3 % and 61.2 % at 400 μg/mL. The IC50 value of var. Eva F1 and var. Hausa were 110 μg/mL and 160 μg/mL. The inhibition constant (KI) of var. Eva F1 and var. Hausa, were 0.006 and 0.016 μg/mL, respectively, and both extracts showed partial competitive inhibition. Hence, this confirms the phytoprotective and tyrosinase inhibitory properties of tomato plant leaves.

scholarly journals Effect of Foliar Feeding of Gluconate and EDTA Chelated Plant Nutrients on Yield, Chlorophyll Content and Nitrate Reductase Enzyme of Bt Cotton under Rainfed Ecosystem of Marathawada

2020 ◽  
pp. 41-49
Author(s):  
P. H. Gourkhede ◽  
V. D. Patil ◽  
S. A. Adkine
Keyword(s):  
Nitrate Reductase ◽  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Development Stage ◽  
Plant Nutrients ◽  
Bt Cotton ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Grade Ii ◽  
Rainfed Ecosystem

An experiment was conducted to find out the “Effect of foliar feeding of Gluconate and EDTA chelated plant nutrients on yield, Chlorophyll content and Nitrate reductase enzyme activity of Bt-cotton under rainfed ecosystem of Marathawada” at Department of Soil Science and Agril Chemistry, VNMKV, Parbhani. The experiment includes sixteen treatments viz,T1-control,T2-ZnGluconate, T3-Zn EDTA, T4-Mn gluconate, T5- Mn EDTA, T6- Cu Gluconate, T7- Cu EDTA, T8- FeGluconate ,T9- Fe EDTA, T10- CaGluconate, T11- Ca EDTA, T12-MgGluconate, T13-MgEDTA, T14- Zn, Mn, Cu, Fe, Ca and Mg Gluconate, T15-Zn, Mn, Cu, Fe, Ca and Mg EDTA and T16- Govt. grade II and replicated twice. The treatments were fertilized with 120:60:60 N,P2O5 and K2O Kg ha-1 .Micronutrient sprays of gluconate and EDTA chelated plant nutrients were applied to the crop at the time of flowering i.e. at 55 DAS and second spray was applied at the time of boll development stage i.e.at 75 DAS. The treatment T2 showed more number of bolls per plant followed by treatment T3. The maximum boll weight was observed with treatment Zn gluconate. Spraying of Zn gluconate, Zn EDTA and Fe and Mg gluconate nutrients have produced more seed cotton yield. Quantitative analysis of chlorophyll was done by using DMSO as an extractant. Chlorophyll a, chlorophyll b and total chlorophyll content in leaves also influenced significantly due to different foliar feeding. The highest chlorophyll a, chlorophyll b and total chlorophyll was registered with the treatment Fe gluconate spray followed by Fe EDTA. Nitrate reductage and acid phosphate activity were improved by the application of T2 and was found to be significantly superior over control. This study was conducted under rainfed ecosystem.

Effects of Phosphorus Stress on Photosynthetic Pigments in Sagittaria sagittifolia

2014 ◽  
Vol 1073-1076 ◽  
pp. 118-121
Author(s):  
Qing Qing He ◽  
Zhou Li Liu ◽  
Wei Chen ◽  
Lin Hao ◽  
Jiao Jiao Qin ◽  
...  
Keyword(s):  
Chlorophyll Content ◽  
Chlorophyll A ◽  
Photosynthetic Pigments ◽  
Carotenoid Content ◽  
Chlorophyll B ◽  
Total Chlorophyll ◽  
Total Chlorophyll Content ◽  
Phosphorus Stress ◽  
Sagittaria Sagittifolia ◽  
Chlorophyll A Content

The photosynthetic pigments of Sagittaria sagittifolia, including total chlorophyll content, chlorophyll a content, chlorophyll b content and carotenoid content, were investigated after 0, 7, 14 and 21 d exposure to 3 mg L-1 P. After 7 d P-exposure, total chlorophyll content, chlorophyll a content and chlorophyll b content increased significantly compared with the control, and then decreased with time extended. Carotenoid content increased significantly after 14 d P-exposure compared with the control, and then decreased slightly after 21 d P-exposure.

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