Manufacturing Characteristics of Probiotic Esherichia Coli Strain M-17 After Immobilization in Gel Carriers and Storage at Various Low Temperatures

cryopreservation of dormant, vegetative apple buds at the National Seed Storage Laboratory is used to maintain a base collection for germplasm held in the National Clonal Germplasm Repository for apple and grape, Geneva, NY, and is performed by a method previously reported1. Growth of buds after grafting is now used to test for survival after exposure to and storage at very low temperatures (ca.-160°C). We are interested in determining if measures of respiration can be used to assess 1. the status of buds and bark used for preservation, 2. survival after different treatments related to cryopreservation, and 3. the extent of sublethal injury after treatments. A Licor 6252 CO2 analyzer was used to measure respiration. Reproducible measurements of respiration required at least 2-3 buds. Buds from winter harvested twigs (ca 45-48% moisture content) that were briefly warmed to room temperature respired at a rate of 34 umoles CO2 g-1 (dw) hr-1. Survival of buds is enhanced if twigs are dried prior to cooling. We found such treatments reduced respiration over non-dried controls. Respiration increased as the bud was rehydrated. Buds from dried twigs slowly cooled to low temperatures had levels of respiration after warming and rehydrating similar to undried, unfrozen controls. Buds from undried twigs directly placed at -196°C and warmed gave little CO2 production.

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The effect of storage temperature and time on total phenolics and enzymatic activity of sapodilla (Achras sapota L.)

Revista Facultad Nacional de Agronomía Medellín ◽

10.15446/rfna.v69n2.59140 ◽

2016 ◽

Vol 69 (2) ◽

pp. 7955-7963 ◽

Cited By ~ 6

Author(s):

Jairo Mercado Camargo ◽

Arnulfo Taron Dunoyer ◽

Luis A. García-Zapateiro

Keyword(s):

Enzymatic Activity ◽

Low Temperatures ◽

Storage Temperature ◽

Total Phenolics ◽

Optimal Parameters ◽

Control Fruit ◽

Maximum Value ◽

Achras Sapota ◽

And Storage ◽

Storage Temperatures

The tropical fruits are sensitive to low storage temperatures, so optimal parameters have been searched for storage and transport for the purpose of maintaining its overall quality as long as possible to the consumer. The effect of different storage temperatures (6, 10, 15, 21 and 27 °C) and storage durations (0 to 20 d) on total phenolics and enzymatic activity of peroxidase (POD), catalase (CAT), and polyphenol oxidase (PPO) on sapodilla (Achras sapota L.) fruit was investigated. The extraction and quantitation of protein and phenols from fruit was performed, then the enzymatic activity of PPO, POD and CAT was determined. The concentration of total phenolics decreased in the control fruit. POD activity was 3268.7 ± 1.4 U g-1 in ripening and senescence of sapodilla stored at 27 °C. CAT activity reached a peak of 34.0 ± 0.25 U g-1 in senescence in control fruit. PPO activity remained unchanged in the ripening stage and until consumption. The best storage temperatures to prolong the post-harvest life of the sapodilla fruit were 6 °C and 10 °C when storage was at low temperatures. POD activity was inactivated during sapodilla storage at low temperatures (6 and 10 °C) and after being transferred to 27 °C the activity was reactivated. Likewise of fruits stored at 21 °C after being transferred to 27 °C the POD activity was reactive with a maximum value of 46.3 ± 0.012 U g-1. Enzyme activity decreased at low temperatures, which contributed to the preservation of the fruit, showing that the cold retards the maturation processes.

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PRESERVATION OF APRICOT PIECES USING EDIBLE COATINGS OR OSMOTIC SOLUTIONS AND STORAGE UNDER LOW TEMPERATURES

Acta Horticulturae ◽

10.17660/actahortic.2006.717.75 ◽

2006 ◽

pp. 373-376

Author(s):

M. Chatzi ◽

V. Fragopoulou ◽

V. Giannou ◽

C. Tzia ◽

I. Karayiannis

Keyword(s):

Low Temperatures ◽

Edible Coatings ◽

And Storage

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Seed dormancy and storage behaviour in tropical Fabaceae: a study of 100 species from Sri Lanka

Seed Science Research ◽

10.1017/s0960258513000214 ◽

2013 ◽

Vol 23 (4) ◽

pp. 257-269 ◽

Cited By ~ 28

Author(s):

K.M.G. Gehan Jayasuriya ◽

Asanga S.T.B. Wijetunga ◽

Jerry M. Baskin ◽

Carol C. Baskin

Keyword(s):

Sri Lanka ◽

Moisture Content ◽

Seed Dormancy ◽

Low Temperatures ◽

Physical Dormancy ◽

The World ◽

And Storage

AbstractSeeds of legumes are generally considered to have physical dormancy and to be orthodox, but most seed biologists are unaware of the various kinds and combinations of dormancy and storage behaviour in seeds of this family. The aim of our study was to document the dormancy and storage behaviour of seeds of 100 native and introduced tropical Fabaceae species in Sri Lanka and classify them into germination/storage behaviour categories. Moisture content (MC) was < 16% for fresh seeds of 94 species and >29% for those of six. Seeds of these six species had low tolerance for desiccation and for low temperatures. Thus, seeds of six species are non-orthodox and 94 species orthodox. Nine of the 100 species were non-dormant, and 2, 3 and 86 had physiological, physiological epicotyl and physical dormancy, respectively. Six germination/storage behaviour categories were identified among the 100 species. However, as in extratropical regions of the world, orthodox storage behaviour and physical dormancy are characteristic of seeds of the majority of species of Fabaceae in tropical Sri Lanka.

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The antimutagenic effects of human saliva investigated with the umu-test. I. Effects of filtration and storage at low temperatures

Mutation Research/Environmental Mutagenesis and Related Subjects ◽

10.1016/0165-1161(91)90298-m ◽

1991 ◽

Vol 252 (1) ◽

pp. 101

Author(s):

M. Okada ◽

S. Nakamura ◽

K. Miura ◽

K. Morimoto

Keyword(s):

Low Temperatures ◽

Human Saliva ◽

Umu Test ◽

And Storage

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Functional Research on Three Presumed Asparagine Synthetase Family Members in Poplar

Genes ◽

10.3390/genes10050326 ◽

2019 ◽

Vol 10 (5) ◽

pp. 326 ◽

Cited By ~ 4

Author(s):

Chunpu Qu ◽

Bingqing Hao ◽

Xiuyue Xu ◽

Yuchen Wang ◽

Chengjun Yang ◽

...

Keyword(s):

Family Members ◽

Ammonium Nitrogen ◽

Coli Strain ◽

Asparagine Synthetase ◽

Plant Nitrogen ◽

Expression Levels ◽

Key Enzyme ◽

And Storage ◽

Different Tissues

Asparagine synthetase (AS), a key enzyme in plant nitrogen metabolism, plays an important role in plant nitrogen assimilation and distribution. Asparagine (Asn), the product of asparagine synthetase, is one of the main compounds responsible for organic nitrogen transport and storage in plants. In this study, we performed complementation experiments using an Asn-deficient Escherichia coli strain to demonstrate that three putative asparagine synthetase family members in poplar (Populus simonii × P. nigra) function in Asn synthesis. Quantitative real-time PCR revealed that the three members had high expression levels in different tissues of poplar and were regulated by exogenous nitrogen. PnAS1 and PnAS2 were also affected by diurnal rhythm. Long-term dark treatment resulted in a significant increase in PnAS1 and PnAS3 expression levels. Under long-term light conditions, however, PnAS2 expression decreased significantly in the intermediate region of leaves. Exogenous application of ammonium nitrogen, glutamine, and a glutamine synthetase inhibitor revealed that PnAS3 was more sensitive to exogenous glutamine, while PnAS1 and PnAS2 were more susceptible to exogenous ammonium nitrogen. Our results suggest that the various members of the PnAS gene family have distinct roles in different tissues and are regulated in different ways.

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