scholarly journals Mechanisms in Tridax procumbens leaf extract reversal of paroxetine-induced erectile dysfunction in corpus cavernosum of male Wistar rats

2021 ◽  
Vol 8 (6) ◽  
pp. 4405-4416
Author(s):  
Shakiru Ademola Salami ◽  
Hussein Mofomosara Salahdeen ◽  
Zainab Ashabi Balogun ◽  
Babatunde Adekunle Murtala ◽  
Yinusa Raji
Keyword(s):  
Erectile Dysfunction ◽  
Wistar Rats ◽  
Contractile Activity ◽  
Corpus Cavernosum ◽  
Calcium Sensitivity ◽  
Oxidase Inhibitor ◽  
K Channel ◽  
Contractile Responses ◽  
Tridax Procumbens ◽  
Male Wistar Rats

Introduction: Aqueous leave extract of Tridax procumbens (AETPL) is reported to improve erectile functions; however, the mechanism is unclear. This study investigates the mechanism involved in the contractile activity of the corpus cavernosum after AETPL treatment of paroxetine-induced erectile dysfunctional adult male Wistar rats. Methods: A total of 20 male Wistar rats were categorized into four groups of five and treated orally for four weeks: Group 1 (distilled water), Group 2 (paroxetine 10 mg/kg), Group 3 (paroxetine + AETPL 100 mg/kg), and Group 4 (paroxetine + Viagra 0.5 mg/kg). Contractile responses of excised corpus cavernosum strips (CS) were determined in response to acetylcholine (ACh), phenylephrine (PHE), potassium chloride (KCl), and calcium chloride (CaCl2), and after incubation in L-NAME, indomethacin, nifedipine, adenosine, caffeine, nicorandil, and acetovanillone. Results: The relaxation response (%) of CS to ACh was significantly inhibited in the paroxetine group compared to the AETPL- and the Viagra-co-treated group. Pre-incubation in L-NAME considerably enhanced the percentage relaxation in groups co-treated with AETPL and Viagra. Groups co-treated with AETPL and Viagra significantly inhibited contraction in response to cumulative doses of CaCl2. Contractile responses of CS to cumulative doses of PHE after incubation in caffeine and adenosine were considerably inhibited in groups co-treated with AETPL and Viagra. Similarly, nicorandil (10-4 M) enhanced the percentage relaxation to cumulative doses of ACh (10-9 — 10-5 M) in groups co-treated with AETPL and Viagra. The pre-incubation of CS with acetovanillone (10-4 M) enhanced the percentage relaxation to ACh across groups. Conclusion: Erectile dysfunction was reversed by AETPL-induced antioxidant/NADPH oxidase inhibitor activity, reduced calcium sensitivity, activation of ATP-sensitive K+ channel, and endothelial Nitric Oxide (NO) release.  

Insulin-induced endothelin release and vasoreactivity in hypertriglyceridemic and hypertensive rats

1999 ◽  
Vol 277 (1) ◽  
pp. H399-H404 ◽  
Author(s):  
Pilar Nava ◽  
Verónica Guarner ◽  
Rosalinda Posadas ◽  
Israel Pérez ◽  
Guadalupe Baños
Keyword(s):  
Drinking Water ◽  
Receptor Antagonist ◽  
Wistar Rats ◽  
Receptor Blocker ◽  
Hypertensive Rats ◽  
Contractile Responses ◽  
Femoral Arteries ◽  
Male Wistar Rats

Insulin-elicited endothelin release in hypertriglyceridemic, hypertensive, hyperinsulinemic (HTG) rats was shown. Weanling male Wistar rats were given 30% sucrose in their drinking water for 20–24 wk. In vitro contractions of aorta and femoral arteries were elicited with 40 mM KCl. Endothelin release induced with KCl plus 50 μU/ml insulin resulted in increases in contractile responses: 41 ± 5.9 and 57 ± 6% for control and 65.5 ± 6 and 95 ± 9% for HTG aortas and femoral arteries, respectively. The endothelin ETB-receptor blocker BQ-788 decreased responses to KCl + insulin by 39 ± 8 and 53 ± 5% in control and 48 ± 13 and 79 ± 3.5% in HTG aortas and femoral arteries, respectively. The ETA-receptor antagonist PD-151242 inhibited these responses by 12 ± 10 and 1 ± 9% in control and by 51.5 ± 9 and 58.5 ± 1% in HTG aortas and femoral arteries, respectively. These results suggest that endothelin may contribute to the hypertension in this model.

Effects of aqueous leaf extract of Tridax procumbens on contractile activity of corpus cavernosum in N -nitro- l -arginine methyl ester-induced hypertensive male rats

2018 ◽  
Vol 16 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Shakiru Ademola Salami ◽  
Hussein Mofomosara Salahdeen ◽  
Evangelshane Chukwudubem Ugbebor ◽  
Babatunde Adekunle Murtala ◽  
Yinusa Raji
Keyword(s):  
Methyl Ester ◽  
Leaf Extract ◽  
Contractile Activity ◽  
Corpus Cavernosum ◽  
Male Rats ◽  
Tridax Procumbens ◽  
Aqueous Leaf Extract

scholarly journals Preliminary Phytochemical Screening of Plumbagozeylanica L. Roots and Its Aphrodisiac Effect in Male Rats

2021 ◽  
pp. 24-31
Author(s):  
Patrick Onen ◽  
Daniel Ocira ◽  
Timothy Omara ◽  
Jacob Nyeko ◽  
Augastine Okwir
Keyword(s):  
Erectile Dysfunction ◽  
Wistar Rats ◽  
Research Work ◽  
Male Rats ◽  
Aqueous Extracts ◽  
Phytochemical Screening ◽  
Plumbago Zeylanica ◽  
Root Extracts ◽  
Methanolic Extracts ◽  
Male Wistar Rats

Aim: To perform phytochemical screening of Plumbago zeylanica L. root extracts and assess the claim of its use in traditional management of erectile dysfunction in Uganda through evaluation of the aphrodisiac effect of its aqueous extracts in male Wistar rats. Study Design: This study employed both qualitative and quantitative research designs. Place and Duration of Study: All research work were performed at the Department of Biological Sciences, Gulu University, Gulu, Uganda between August 2019 and December 2019. Methodology: Classical phytochemical screening of aqueous and methanolic extracts of Plumbago zeylanica roots were performed following standard methods. Aqueous extracts were administered to Male Wistar rats and the effect of the extracts on the mounting and intromission frequencies were determined. Results: Glycosides, phenols, saponins, quinones, terpenoids and steroids were present in both methanolic and aqueous extracts. Alkaloids were present in methanolic extracts only while tannins and phlobatannins were only present in aqueous extracts. Flavonoids, coumarins and anthraquinones were not detected in both extracts. Aqueous root extracts of Plumbago zeylanica produced pro-sexual stimulatory effects in male rats when administered at 150, 300 and 450 mg/kg body weight. Conclusion: The results support the use of Plumbago zeylanicaroots by indigenous people in Uganda to increase libido, treat premature ejaculation and erectile dysfunction. The extracts had low sexual enhancement in sexually inexperienced male rats and therefore, further studies using experienced animal models are needed to better apprehend the prosexual effects of P. zeylanica roots. The toxicity of the extracts as well as structural elucidation and pharmacological evaluation of the responsible bioactive compounds merit further studies.

Inhibition of glycogenesis in rat muscles partially depleted of glycogen

1991 ◽  
Vol 261 (2) ◽  
pp. C305-C309 ◽  
Author(s):  
J. Gorski ◽  
I. Krawczuk ◽  
M. Gorska ◽  
J. Rutkiewicz
Keyword(s):  
Sciatic Nerve ◽  
Wistar Rats ◽  
Contractile Activity ◽  
Muscle Group ◽  
Glycogen Depletion ◽  
Stomach Tube ◽  
Plantaris Muscle ◽  
Anesthetized Rats ◽  
Male Wistar Rats ◽  
Stimulation Of

This study aimed to examine the extent to which repletion of glycogen in muscles partially depleted of glycogen may be inhibited by contractions and epinephrine. Four experiments were carried out on untrained male Wistar rats. 1) Animals ran 150 min (1,200 m/h) on a treadmill set at +10 degrees incline. After 60, 90, and 120 min of running, they were given glucose (40% solution, 1 ml/100 g) by a stomach tube. 2) Rats ran on a treadmill set as above for 20 min at 3,200 m/h and then 2 h at 800 m/h. 3) In anesthetized rats, gastrocnemius-plantaris muscle group was made to contract isometrically by means of stimulation of the sciatic nerve. The nerve was stimulated with tetanic pulses (7 V, 0.05-ms duration, delivered in 100-ms trains at 100 Hz): first 15 min, 2 pulses/s, then 2 h, 1 pulse/2 s. 4) Epinephrine (0.5 mg/kg) was administered subcutaneously 10 min before onset of stimulation of the sciatic nerve. The nerve was stimulated 15 min with tetanic pulses (as above), 2 pulses/s. Next, rats were rested for 2 h. Level of glycogen was determined in samples of white and red gastrocnemius, plantaris, and soleus. Initial running or stimulation resulted in pronounced glycogen depletion in each muscle. Thereafter, either considerable or full repletion of glycogen occurred in the muscles despite continued contractile activity or the presence of epinephrine. The degree of repletion depended both on the type of muscle and the type of activation of glycogenolysis (running, stimulation of the nerve, epinephrine).(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals High-Salt Intake Reduces Apomorphine-Induced Penile Erection and Increases Neurally Mediated Contractile Responses of the Cavernosal Smooth Muscle in Rats

2019 ◽  
Vol 32 (12) ◽  
pp. 1206-1213
Author(s):  
Amanda Leitolis ◽  
Sandra Crestani ◽  
Áurea Elizabeth Linder ◽  
José Eduardo da Silva-Santos
Keyword(s):  
Electrical Stimulation ◽  
Erectile Dysfunction ◽  
Penile Erection ◽  
Blood Pressure Measurement ◽  
Corpus Cavernosum ◽  
Extracellular Calcium ◽  
High Salt ◽  
Control Group ◽  
Contractile Responses ◽  
High Salt Intake

Abstract BACKGROUND This study was designed to evaluate whether overconsumption of NaCl, a well-known risk factor for hypertension, leads to erectile dysfunction in rodents. METHODS Male Wistar rats received regular chow (control group) or 4% NaCl chow for 24 weeks and were subjected to blood pressure measurement and apomorphine-induced erection. Moreover, cavernosal strips from both the control and 4% NaCl groups were evaluated in organ baths. RESULTS Animals subjected to 4% NaCl chow did not develop hypertension but presented a significant reduction in the total number of erections following apomorphine administration as compared with the control group. The addition of high KCl or phenylephrine resulted in similar contractile responses in the corpus cavernosal strips from both the control and 4% NaCl groups. However, electrical field stimulation-induced contraction was significantly enhanced in cavernosal strips from animals exposed to 4% NaCl. Incubation of Y-27632, but not of atropine and Nω-nitro-l-arginine methyl ester (L-NAME), entirely prevented the potentiation of the contractile responses evoked by electrical stimulation. The enhanced contractile responses evoked by electrical stimulation found in the high-salt group were also avoided in the absence of extracellular calcium. Concentration-response curves of CaCl2 revealed augmented contractility in response to extracellular calcium in cavernosal strips from the 4% NaCl-treated rats, compared with control samples. CONCLUSIONS A high-salt diet alone rendered the animals less responsive to apomorphine-induced penile erection and enhanced neurally mediated contractile responses in the corpus cavernosum, a clear indication that overconsumption of sodium can lead to erectile dysfunction even without the development of hypertension.

Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

1986 ◽  
Vol 44 ◽  
pp. 342-343
Author(s):  
I. Stachura ◽  
M. Pardo ◽  
J. Costello ◽  
D.M. Landwehr
Keyword(s):  
Wistar Rats ◽  
Renal Damage ◽  
Standard Diet ◽  
Experimental Conditions ◽  
Phosphate Intake ◽  
Dietary Restrictions ◽  
Severe Reduction ◽  
Male Wistar Rats ◽  
Unrestricted Diet ◽  
Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

Augurin stimulates food intake in male Wistar rats

2013 ◽  
pp. 1-1
Author(s):  
Michael Patterson ◽  
John Tadross ◽  
Keisuke Suzuki ◽  
Kylie Beale ◽  
Charoltte Boughton ◽  
...  
Keyword(s):  
Food Intake ◽  
Wistar Rats ◽  
Male Wistar Rats
Sign in / Sign up

Export Citation Format

Share Document