Augurin stimulates food intake in male Wistar rats

Background Adipose and hepatic metabolic dysfunctions are critical comorbidities that also aggravate insulin resistance in obese individuals. Melatonin is a low-cost agent and previous studies suggest that its use may promote metabolic health. However, its effects on some comorbidities associated with obesity are unknown. Herein, we investigated the hypothesis that melatonin supplementation would attenuate adipose-hepatic metabolic dysfunction in high fat diet (HFD)-induced obesity in male Wistar rats. Materials and methods Twenty-four adult male Wistar rats (n = 6/group) were used: Control group received vehicle (normal saline), obese group received 40% high fat diet, melatonin-treated group received 4 mg/kg of melatonin, and obese plus melatonin group received 40% HFD and melatonin. The treatment lasted for 12 weeks. Results HFD caused increased food intake, body weight, insulin level, insulin resistance and plasma and liver lipid but decreased adipose lipid. In addition, HFD also increased plasma, adipose and liver malondialdehyde, IL-6, uric acid and decreased Glucose-6-phosphate dehydrogenase, glutathione, nitric oxide and circulating obestatin concentration. However, these deleterious effects except food intake were attenuated when supplemented with melatonin. Conclusion Taken together, the present results indicate that HFD exposure causes adipose-hepatic metabolic disturbance in obese animals, which are accompanied by oxidative stress and inflammation. In addition, the present results suggest that melatonin supplementation attenuates adipose-hepatic metabolic dysfunction, accompanying obesity by suppression of oxidative stress/inflammation-dependent mechanism and increasing circulating obestatin.

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Aqueous extract of Ipomoea batatas reduces food intake in male wistar rats: A pilot study

Annals of Medical and Health Sciences Research ◽

10.4103/2141-9248.139362 ◽

2014 ◽

Vol 4 (4) ◽

pp. 647 ◽

Cited By ~ 1

Author(s):

TH Olubobokun ◽

EO Aluko ◽

EE Iyare ◽

USB Anyaehie ◽

ED Olatunbosun ◽

...

Keyword(s):

Pilot Study ◽

Food Intake ◽

Aqueous Extract ◽

Ipomoea Batatas ◽

Wistar Rats ◽

Male Wistar Rats

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Effect of Subtotal Colectomy on Body Weight and Food Intake in an Experimental Model of Obesity in Male Wistar Rats

The Open Obesity Journal ◽

10.2174/1876823701204010051 ◽

2012 ◽

Vol 4 (1) ◽

pp. 51-54

Author(s):

Pilar Fernandez-Mateos

Keyword(s):

Body Weight ◽

Food Intake ◽

Experimental Model ◽

Subtotal Colectomy ◽

Wistar Rats ◽

Male Wistar Rats

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Characterization of feeding behavior induced by central injection of GRF

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1990.259.3.r651 ◽

1990 ◽

Vol 259 (3) ◽

pp. R651-R657 ◽

Cited By ~ 2

Author(s):

P. R. Dickson ◽

F. J. Vaccarino

Keyword(s):

Growth Hormone ◽

Food Intake ◽

Suprachiasmatic Nucleus ◽

Paraventricular Nucleus ◽

Wistar Rats ◽

Preoptic Area ◽

Behavioral Responses ◽

Behavioral Measures ◽

Male Wistar Rats

The behavior resulting from injection of rat hypothalamic growth hormone-releasing factor (GRF) (0, 0.01, 0.1, 1.0 pmol) into the suprachiasmatic nucleus-medial preoptic area (SCN/MPOA) or the paraventricular nucleus (PVN) of the hypothalamus was examined. After injections of GRF, feeding and other behavioral responses of male Wistar rats were observed for 90 min. In the SCN/MPOA, GRF dose dependently increased food intake, increasing mean meal length at 0.1 pmol and increasing rate of eating with no effect on meal length at 1.0 pmol. Other behavioral measures were unaffected by GRF. There was no effect after injections into the PVN. These data are taken as confirmation that the SCN/MPOA region of the hypothalamus is important for the central stimulatory effects of GRF on feeding. The possibility that the PVN is involved in the expression of feeding derived from intra-SCN/MPOA GRF injections is discussed.

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Hydroxycitric acid ameliorates high-fructose-induced redox imbalance and activation of stress sensitive kinases in male Wistar rats

Journal of Basic and Clinical Physiology and Pharmacology ◽

10.1515/jbcpp-2014-0132 ◽

2016 ◽

Vol 0 (0) ◽

Author(s):

Ramalingam Sripradha ◽

Magadi Gopalakrishna Sridhar ◽

Nachimuthu Maithilikarpagaselvi

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Food Intake ◽

Wistar Rats ◽

Density Lipoprotein ◽

Mitogen Activated Protein Kinase ◽

Stress Index ◽

Redox Imbalance ◽

Hydroxycitric Acid ◽

Male Wistar Rats

AbstractExcess fructose consumption causes dyslipidemia, oxidative stress, and various complications. Hydroxycitric acid (HCA), one of the principal components of the fruitMale Wistar rats (n=40) were randomly divided into four groups with 10 rats in each group. The rats were fed with either standard rodent diet or 60% fructose diet and administered with HCA at a dose of 400 mg/kg body wt/day for 10 weeks. Body weight was measured once a week, and food intake was noted daily. At the end of the study, lipid profile and oxidative stress parameters were estimated. Expressions of stress sensitive kinases were analyzed in liver homogenates.Fructose-fed rats displayed elevated body weight, higher levels of plasma total cholesterol (TC), triacylglycerol (TAG), non-high-density lipoprotein cholesterol (non HDL-C), malondialdehyde (MDA), total oxidant status (TOS), oxidative stress index (OSI), lower levels of HDL-C, glutathione (GSH), glutathione peroxidase (GPx), and total antioxidant status (TAS). Fructose feeding caused higher phosphorylation of stress sensitive kinases ERK ½ and p38. Administration with HCA lowered body weight, food intake, TAG, non-HDL-C, MDA, TOS, and OSI and elevated GSH, GPx, and TAS levels. Reduced phosphorylation of ERK ½ and p38 mitogen-activated protein kinase (MAPK) was observed upon HCA treatment.Thus, HCA improved fructose induced redox imbalance and activation of stress sensitive kinases through its hypolipidemic effects.

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One-month of high-intensity exercise did not change the food intake and the hypothalamic arcuate nucleus proopiomelanocortin and neuropeptide Y expression levels in male Wistar rats

Endocrine Regulations ◽

10.2478/enr-2019-0002 ◽

2019 ◽

Vol 53 (1) ◽

pp. 8-13 ◽

Cited By ~ 1

Author(s):

Nazli Khajehnasiri ◽

Homayoun Khazali ◽

Farzam Sheikhzadeh ◽

Mahnaz Ghowsi

Keyword(s):

Body Weight ◽

Food Intake ◽

Mrna Expression ◽

High Intensity ◽

Wistar Rats ◽

Arcuate Nucleus ◽

The Body ◽

High Intensity Exercise ◽

Hypothalamic Arcuate Nucleus ◽

Male Wistar Rats

AbstractObjective. The hypothalamic arcuate nucleus proopiomelanocortin (POMC) and neuropeptide Y (NPY) circuitries are involved in the inhibition and stimulation of the appetite, respectively. The aim of this study was to investigate the effects of one-month lasting high-intensity exercise on the POMC mRNA and NPY mRNA expression in the above-mentioned brain structure and appetite and food intake levels.Methods. Fourteen male Wistar rats (250±50 g) were used and kept in the well-controlled conditions (22±2 °C, 50±5% humidity, and 12 h dark/light cycle) with food and water ad libitum. The rats were divided into two groups (n=7): 1) control group (C, these rats served as controls) and 2) exercised group (RIE, these rats performed a high-intensity exercise for one month (5 days per week) 40 min daily with speed 35 m/min. The total exercise time was 60 min. The body weight and food intake were recorded continuously during the experiments.Results. The results showed relative mRNA expression of POMC and NPY estimated in the hypothalamic arcuate nucleus. There were no significant differences in the NPY and POMC mRNAs expression levels and food intake between C and RIE groups.Conclusions. The present data indicate that one-month regular intensive exercise did not alter the levels of NPY and POMC mRNAs expression (as two important factors in the regulation of appetite) in the hypothalamic arcuate nucleus and food intake suggesting that this type of exercise itself is not an appropriate procedure for the body weight reduction.

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Allopregnanolone preferentially induces energy-rich food intake in male Wistar rats

Physiological Reports ◽

10.14814/phy2.12190 ◽

2014 ◽

Vol 2 (12) ◽

pp. e12190 ◽

Cited By ~ 6

Author(s):

Ellinor Holmberg ◽

Maja Johansson ◽

Torbjörn Bäckström ◽

David Haage

Keyword(s):

Food Intake ◽

Wistar Rats ◽

Rich Food ◽

Male Wistar Rats

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Central Relaxin-3 Administration Causes Hyperphagia in Male Wistar Rats

Endocrinology ◽

10.1210/en.2004-1532 ◽

2005 ◽

Vol 146 (8) ◽

pp. 3295-3300 ◽

Cited By ~ 125

Author(s):

B. M. C. McGowan ◽

S. A. Stanley ◽

K. L. Smith ◽

N. E. White ◽

M. M. Connolly ◽

...

Keyword(s):

Food Intake ◽

Wistar Rats ◽

Dark Phase ◽

Central Administration ◽

Light Phase ◽

Male Wistar Rats ◽

The Central Nervous System ◽

Intracerebroventricular Injections ◽

Relaxin 2 ◽

G Protein Coupled

Abstract Relaxin-3 (INSL-7) is a recently discovered member of the insulin superfamily. Relaxin-3 mRNA is expressed in the nucleus incertus of the brainstem, which has projections to the hypothalamus. Relaxin-3 binds with high affinity to the LGR7 receptor and to the previously orphan G protein-coupled receptor GPCR135. GPCR135 mRNA is expressed predominantly in the central nervous system, particularly in the paraventricular nucleus (PVN). The presence of relaxin-3 and these receptors in the PVN led us to investigate the effect of central administration of relaxin-3 on food intake in male Wistar rats. The receptor involved in mediating these effects was also investigated. Intracerebroventricular injections of human relaxin-3 (H3) to satiated rats significantly increased food intake 1 h post administration in the early light phase [0.96 ± 0.16 g (vehicle) vs. 1.81 ± 0.21 g (180 pmol H3), P < 0.05] and the early dark phase [2.95 ± 0.45 g (vehicle) vs. 4.39 ± 0.39 g (180 pmol H3), P < 0.05]. Intra-PVN H3 administration significantly increased 1-h food intake in satiated rats in the early light phase [0.34 ± 0.16 g (vehicle) vs. 1.23 ± 0.30 g (18 pmol H3), P < 0.05] and the early dark phase [4.43 ± 0.32 g (vehicle) vs. 6.57 ± 0.42 g (18 pmol H3), P < 0.05]. Feeding behavior increased after intra-PVN H3. Equimolar doses of human relaxin-2, which binds the LGR7 receptor but not GPCR135, did not increase feeding. Hypothalamic neuropeptide Y, proopiomelanocortin, or agouti-related peptide mRNA expression did not change after acute intracerebroventricular H3. These results suggest a novel role for relaxin-3 in appetite regulation.

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Pulsatile secretion of growth hormone and insulin in relation to feeding in rats

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.1987.253.5.r772 ◽

1987 ◽

Vol 253 (5) ◽

pp. R772-R778

Author(s):

P. Even ◽

J. Danguir ◽

S. Nicolaidis ◽

C. Rougeot ◽

F. Dray

Keyword(s):

Growth Hormone ◽

Insulin Secretion ◽

Food Intake ◽

Wistar Rats ◽

Plasma Concentrations ◽

Endocrine Activity ◽

Blood Samples ◽

Gh Secretion ◽

Male Wistar Rats ◽

Intracardiac Catheters

In unrestrained male Wistar rats chronically implanted with intracardiac catheters, blood samples were taken every 20 min throughout the 24 h of the diurnal cycle. Plasma concentrations of growth hormone (GH), insulin, and glucose were measured. The pattern of food intake was continuously monitored. The existence of 3-h pulsatile cycles of GH secretion was confirmed. In addition, short bursts of insulin secretion were observed in the middle of every second GH peak-to-peak interval. Food intake appeared to be enhanced during short periods that corresponded with GH release into the blood and was reduced during the GH peak-to-peak periods in which the bursts of insulin secretion were observed. From these observations this study draws a schematic relationship between the rhythmicity of the secretion of GH and insulin and the probability of occurrence of feeding. We speculate that the rhythmic endocrine activity may be causally related to feeding.

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