Effects of Gravity on Tracheal Mucus Transport Rates in Normal Subjects and in Patients With Cystic Fibrosis

PEDIATRICS ◽  
1977 ◽  
Vol 60 (2) ◽  
pp. 146-152
Author(s):  
John W. Wong ◽  
Thomas G. Keens ◽  
Eleanor M. Wannamaker ◽  
Douglas N. Crozier ◽  
Henry Levison ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Normal Subjects ◽  
Major Influence ◽  
Upper Respiratory Tract ◽  
Mucus Clearance ◽  
Effective Form ◽  
Postural Drainage ◽  
Significant Difference ◽  
The Subject ◽  
Tracheal Mucus

A noninvasive, radionuclide imaging technique for measuring the rate of mucus clearance in the trachea (RT), was used to study gravitational effects on mucus clearance in 13 patients with cystic fibrosis (CF), average age 17 years; 7 normal, nonsmoking adults, average age 26 years; and a normal subject who was recovering from an acute upper respiratory tract infection (URTI). In the upright position, nine of the CF patients and the subject with URTI demonstrated abnormal tracheal mucus clearance which approached normal when they were placed in a 25° headdown position. The normal subjects and two of the CF patients showed no significant difference in the RT measured in the two positions. The results of the study indicate that the force of gravity can be a major influence on tracheal mucus clearance in CF and URTI subjects. This conclusion supports the use of postural drainage as an effective form of therapy in patients with cystic fibrosis.

scholarly journals Serine Proteases Degrade Airway Mucins in Cystic Fibrosis

2011 ◽  
Vol 79 (8) ◽  
pp. 3438-3444 ◽  
Author(s):  
Markus O. Henke ◽  
Gerrit John ◽  
Christina Rheineck ◽  
Shashi Chillappagari ◽  
Lutz Naehrlich ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Serine Proteases ◽  
Defense Mechanism ◽  
Normal Subjects ◽  
Human Neutrophil Elastase ◽  
Content Type ◽  
Significant Difference ◽  
History Of ◽  
Serine Proteases Inhibitors ◽  
Normal Controls

ABSTRACTAirway mucins are the major molecular constituents of mucus. Mucus forms the first barrier to invading organisms in the airways and is an important defense mechanism of the lung. We confirm that mucin concentrations are significantly decreased in airway secretions of subjects with cystic fibrosis (CF) who have chronicPseudomonas aeruginosainfection. In sputum from CF subjects without a history ofP. aeruginosa, we found no significant difference in the mucin concentration compared to mucus from normal controls. We demonstrate that mucins can be degraded by synthetic human neutrophil elastase (HNE) andP. aeruginosaelastase B (pseudolysin) and that degradation was inhibited by serine proteases inhibitors (diisopropyl fluorophosphates [DFP], phenylmethylsulfonyl fluoride [PMSF], and 1-chloro-3-tosylamido-7-amino-2-heptanone HCl [TLCK]). The mucin concentration in airway secretions from CF subjects is similar to that for normal subjects until there is infection byP. aeruginosa, and after that, the mucin concentration decreases dramatically. This is most likely due to degradation by serine proteases. The loss of this mucin barrier may contribute to chronic airway infection in the CF airway.

scholarly journals Hydrogen peroxide in breath condensate during a common cold

2001 ◽  
Vol 10 (6) ◽  
pp. 351-354 ◽  
Author(s):  
Rijn Q. Jöbsis ◽  
Susanne L. Schellekens ◽  
Anoeska Fakkel-Kroesbergen ◽  
Rolien H. C. Raatgeep ◽  
Johan C. de Jongste
Keyword(s):  
Hydrogen Peroxide ◽  
Respiratory Tract ◽  
Common Cold ◽  
Normal Subjects ◽  
Forced Expiratory Volume ◽  
Lower Airway ◽  
Upper Respiratory Tract ◽  
Significant Difference ◽  
Exhaled Air ◽  
Breath Condensate

Background:Hydrogen peroxide (H2O2) in exhaled air condensate is elevated in inflammatory disorders of the lower respiratory tract. It is unknown whether viral colds contribute to exhaled H2O2.Aim:To assess exhaled H2O2during and after a common cold.Methods:We examined H2O2in the breath condensate of 20 normal subjects with acute symptoms of a common cold and after recovery 2 weeks later and, similarly, in 10 subjects without infection. H2O2was measured with a fluorimetric assay.Results:At the time of infection exhaled H2O2(median, ranges) was 0.20 μM (0.03-1.2 μM), and this decreased to 0.09 μM (< 0.01-0.40 μM) after recovery(p=0.006). There was no significant difference in lung function (forced vital capacity and forced expiratory volume in 1 sec) during and after colds. In the controls, exhaled H2O2did not change over a 2-week period.Conclusions:H2O2in exhaled air condensate is elevated during a common cold, and returns to normal within 2 weeks of recovery in healthy subjects. Hence, symptomatic upper respiratory tract infection may act as a confounder in studies of H2O2as a marker of chronic lower airway inflammation.

Differentiation between the intensity of breathlessness and the distress it evokes in normal subjects during exercise

1991 ◽  
Vol 80 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Rachel C. Wilson ◽  
P. W. Jones
Keyword(s):  
Minute Ventilation ◽  
Normal Subjects ◽  
Cycle Ergometer ◽  
Exercise Tests ◽  
Distress Score ◽  
Significant Difference ◽  
Ergometer Exercise ◽  
Wide Scatter ◽  
The Subject ◽  
The Mean

1. This study was designed to examine whether normal subjects could differentiate between the ‘intensity’ of their breathlessness and the amount of ‘distress’ it evoked, by specific wording of the instructions. 2. A preliminary study showed no significant difference between ‘distress’ score during exercise measured on two separate occasions (P = 0.3). 3. Ten subjects each performed two identical incremental cycle-ergometer exercise tests on separate occasions during which they were asked to quantify either ‘intensity’ or ‘distress’ by using modified Borg scales. 4. In all subjects there was a significant correlation (P <0.001) between ‘intensity’ and minute ventilation. In eight subjects there was a significant correlation (P <0.05) between ‘distress’ and minute ventilation. One subject displayed no significant correlation and one registered no distress. 5. Mean ‘intensity’ was greater than the mean ‘distress’ (P = 0.0001). The slope of ‘intensity’/minute ventilation was greater than the slope of ‘distress’/minute ventilation (P = 0.0001). 6. Within individuals there was a significant correlation beween ‘intensity’ and ‘distress’ (P <0.05). There was a wide scatter in the slope of this relationship between subjects and maximum ‘intensity’ and ‘distress’ did not correlate. 7. Different elements of the breathlessness sensation could be identified and selectively measured depending on the wording of the instructions given to the subject. 8. There was a wide intersubject variation in the magnitude of both breathlessness ‘intensity’ and ‘distress’ estimates, but the differences between subjects in these two components of the sensation did not appear to follow a common pattern.

Auditory Selective Attention in Cerebral-Palsied Individuals

1985 ◽  
Vol 16 (4) ◽  
pp. 260-266 ◽  
Author(s):  
Lee Ann Laraway
Keyword(s):  
Selective Attention ◽  
White Noise ◽  
Normal Subjects ◽  
Auditory Selective Attention ◽  
Normal Individuals ◽  
Significant Difference

The purpose of this study was to determine whether there is a statistically significant difference between the auditory selective attention abilities of normal and cerebral-palsied individuals. Twenty-three cerebral-palsied and 23 normal subjects between the ages of 5 and 21 were asked to repeat a series of 30 items consisting of from 2 to 4 digits in the presence of intermittent white noise. Results of the study indicate that cerebral-palsied individuals perform significantly poorer than normal individuals when the stimulus is accompanied by noise. Noise was not a significant factor in the performance of the normal subjects regardless of age.

A Specific Immunologic Assay for Functional Plasminogen Activator Inhibitor 1 in Plasma – Standardized Measurements of the Inhibitor and Related Parameters in Patients with Venous Thromboembolic Disease

1992 ◽  
Vol 68 (05) ◽  
pp. 486-494 ◽  
Author(s):  
Malou Philips ◽  
Anne-Grethe Juul ◽  
Johan Selmer ◽  
Bent Lind ◽  
Sixtus Thorsen
Keyword(s):  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Normal Subjects ◽  
Tissue Type ◽  
Blood Collection ◽  
Plasminogen Activator Inhibitor 1 ◽  
Type Plasminogen Activator ◽  
Significant Difference ◽  
Activator Inhibitor ◽  
Pai 1

SummaryA new assay for functional plasminogen activator inhibitor 1 (PAI-1) in plasma was developed. The assay is based on the quantitative conversion of PAI-1 to urokinase-type plasminogen activator (u-PA)-PAI-l complex the concentration of which is then determined by an ELISA employing monoclonal anti-PAI-1 as catching antibody and monoclonal anti-u-PA as detecting antibody. The assay exhibits high sensitivity, specificity, accuracy, and precision. The level of functional PAI-1, tissue-type plasminogen activator (t-PA) activity and t-PA-PAI-1 complex was measured in normal subjects and in patients with venous thromboembolism in a silent phase. Blood collection procedures and calibration of the respective assays were rigorously standardized. It was found that the patients had a decreased fibrinolytic capacity. This could be ascribed to high plasma levels of PAI-1. The release of t-PA during venous occlusion of an arm for 10 min expressed as the increase in t-PA + t-PA-PAI-1 complex exhibited great variation and no significant difference could be demonstrated between the patients with a thrombotic tendency and the normal subjects.

Comparative Plasma Heparin Levels after Subcutaneous Sodium and Calcium Heparin

1978 ◽  
Vol 40 (02) ◽  
pp. 397-406 ◽  
Author(s):  
Joyce Low ◽  
J C Biggs
Keyword(s):  
Peak Plasma ◽  
Plasma Concentrations ◽  
Factor Xa ◽  
Normal Subjects ◽  
Sodium Salts ◽  
Sodium Heparin ◽  
Significant Difference ◽  
Heparin Plasma ◽  
Calcium Heparin ◽  
Plasma Heparin

SummaryComparative plasma heparin levels were measured in normal subjects injected subcutaneously with 5,000 units of the sodium and calcium salts of heparin. Plasma heparin levels were measured up to 7 hr post-injection by an anti-factor Xa assay (Denson and Bonnar 1973). Preliminary studies indicated that heparin levels were reproducible in subjects who received two injections of the same heparin. Peak plasma concentrations (Cmax) and the time at which peak concentration was reached (Tmax) varied greatly from subject to subject. In one group of subjects (15) two commonly used heparins, a sodium heparin (Evans) and a calcium heparin (Choay) were compared. Peak heparin concentrations were not significantly different. However the Tmax for the sodium heparin (1.5 hr) was significantly earlier than the Tmax for the calcium heparin (3 hr) and this was not due to a difference in the volume of the two heparin injections. No significant difference could be detected in the plasma clearance rate and the molecular weight distribution of the two heparins.In two other groups of subjects, sodium and calcium preparations from two manufacturers were compared. In general, the sodium salts gave rise to significantly higher plasma concentrations, which could be interpreted as a greater bioavailability of sodium salts. These results indicate that the salt of the heparin can influence the plasma concentration achieved after subcutaneous injection.

SENSITIVITAS ETIS MAHASISWA ATAS PENGGELAPAN PAJAK

2017 ◽  
Vol 8 (2) ◽  
pp. 178-195
Author(s):  
Nurma Risa
Keyword(s):  
Tax Evasion ◽  
Female Students ◽  
T Test ◽  
Study Program ◽  
Male And Female ◽  
Political Sciences ◽  
Significant Difference ◽  
The Subject ◽  
And Gender ◽  
Management Students

This study aims to prove that there is a difference of perception about ethics on tax evasion in UNISMA Bekasi students, based on selected study program and gender. The sample of this research is the students who have fulfilled the subject of taxation, at the Faculty of Economics (FE) and Faculty of Social and Political Sciences (FISIP). Using independent t-test, the results showed that there was no significant difference of perception about tax evasion ethics between FE and FISIP students. But significant differences the perception of tax evasion ethics occur between accounting and management students at FE. Significant differences also did not occur between male and female students

A COMPARISON OF TWO METHODS FOR MEASUREMENT OF ALDOSTERONE SECRETION RATE IN MAN

1966 ◽  
Vol 53 (2) ◽  
pp. 177-188 ◽  
Author(s):  
P. Lund-Johansen ◽  
T. Thorsen ◽  
K. F. Støa
Keyword(s):  
Urinary Excretion ◽  
Normal Subjects ◽  
Secretion Rate ◽  
Aldosterone Secretion ◽  
Simple Method ◽  
Mean Values ◽  
Pathological Conditions ◽  
Significant Difference ◽  
Derivative Method ◽  
The Mean

ABSTRACT A comparison has been made between (A), a relatively simple method for the measurement of aldosterone secretion rate, based on paper chromatography and direct densitometry of the aldosterone spot and (B) a more elaborate isotope derivative method. The mean secretion rate in 9 normal subjects was 112 ± 26 μg per 24 hours (method A) and 135 ± 35 μg per 24 hours (method B). The »secretion rate« in one adrenalectomized subject after the intravenous injection of 250 μg of aldosterone was 230 μg per 24 hours (method A) and 294 μg per 24 hours (method B). There was no significant difference in the mean values, and correlation between the two methods was good (r = 0.80). It is concluded that the densitometric method is suitable for clinical purposes as well as research, being more rapid and less expensive than the isotope derivative method. Method A also measures the urinary excretion of the aldosterone 3-oxo-conjugate, which is of interest in many pathological conditions. The densitometric method is obviously the less sensitive and a prerequisite for its use is an aldosterone secretion of 20—30 μg per 24 hours. Lower values are, however, rare in adults.

IMMUNOLOGICAL REACTIVITY OF INSULIN IN HUMAN SERUM

1966 ◽  
Vol 53 (4) ◽  
pp. 673-680 ◽  
Author(s):  
Torsten Deckert ◽  
Kai R. Jorgensen
Keyword(s):  
Normal Subjects ◽  
The Other ◽  
Human Pancreas ◽  
Porcine Pancreas ◽  
Crystalline Insulin ◽  
Endogenous Insulin ◽  
Significant Difference ◽  
Human Sera ◽  
Normal Human ◽  
The One

ABSTRACT The purpose of this study was to investigate whether a difference could be demonstrated between crystalline insulin extracted from normal human pancreas, and crystalline insulin extracted from bovine and porcine pancreas. Using Hales & Randle's (1963) immunoassay no immunological differences could be demonstrated between human and pig insulin. On the other hand, a significant difference was found, between pig and ox insulin. An attempt was also made to determine whether an immunological difference could be demonstrated between crystalline pig insulin and crystalline human insulin from non diabetic subjects on the one hand and endogenous, circulating insulin from normal subjects, obese subjects and diabetic subjects on the other. No such difference was found. From these experiments it is concluded that endogenous insulin in normal, obese and diabetic human sera is immunologically identical with human, crystalline insulin from non diabetic subjects and crystalline pig insulin.

Analisis Perbedaan Prestasi Belajar Mahasiswa Fakultas Tarbiyah Dan Ilmu Keguruan (Ftik) Iain Palu Pada Mata Kuliah Evaluasi Pembelajaran Berdasarkan Gender

2019 ◽  
Vol 9 (1) ◽  
pp. 152-190
Author(s):  
Wiwin Mistiani
Keyword(s):  
Student Achievement ◽  
Sampling Technique ◽  
Female Students ◽  
Male Students ◽  
Error Level ◽  
Learning Achievement ◽  
Learning Evaluation ◽  
Significant Difference ◽  
The Subject ◽  
Evaluation Of Learning

This study aims to determine the student achievement of male and female students on the subyek Evaluation of learning and to determine of Differences in student achievement FTIK IAIN Palu on the subject evaluastion learning by gender. The approach used is descriptive quantitative. While the population in this study are all students. The sampling technique is done by using proportional random sampling technique. With the number of students 60 people then the sample = 37 students of FTIK IAIN Palu. Technique of collecting data by using test and observation while data analysis using T test.From the research, 1) the average of learning achievement in the subject of male students' learning appraisal from 19 male students who are the sample in this research is 79,42 means good category. Meanwhile, from 18 female students who became the sample of the study, the average of learning achievement in the subject of female students' learning evaluation is 77 means either. 2) There is no significant difference Student Learning Achievement FTIK) IAIN Palu On the subject Evaluation Learning Based on Gender with t value equal to = 0,0267 with t table with dk 35 with error level 5% is 0,062.

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