scholarly journals Preliminary study of the enzyme ubiquitin carboxylterminal hydrolase 14 (UBP6) in Giardia intestinalis: structural bioinformatic analysis and transcriptional profile during encystation

2015 ◽  
Vol 43 (2) ◽  
pp. 32-40
Author(s):  
Magda E Alvarado ◽  
Camila A González ◽  
Moisés Wasserman ◽  
Claudia C Rubiano
Keyword(s):  
Protein Turnover ◽  
Bioinformatic Analysis ◽  
Giardia Intestinalis ◽  
Deubiquitinating Enzyme ◽  
Combined Approach ◽  
Protein Coding ◽  
Starting Point ◽  
The Family ◽  
Preliminary Study ◽  
Carboxyl Terminal

This paper presents a combined approach<br />with two aims. The first is to analyze the<br />reported sequence of the enzyme ubiquitin<br />carboxyl-terminal hydrolase 14 of Giardia<br />intestinalis (UBP6) through computational<br />methods to find components related with<br />its hypothetical function. The second is<br />to determine if the protein-coding gene is<br />expressed in G. intestinalis and, if such is<br />the case, also determine its transcription<br />pattern along the life cycle of the parasite. It<br />was established that the protein belongs to<br />the family of Cys-dependent deubiquitinases<br />and more specifically to ubiquitin specific<br />proteases (USPs). Moreover, the catalytic<br />center with the complete triad as well as<br />typical features of the USP motif were also<br />identified. Since the computational findings<br />suggest that the enzyme could be functional,<br />reverse transcription coupled to PCR was<br />used as a first approach to establish if in fact<br />the coding gene is expressed in the parasite.<br />Interestingly, it was found not only that<br />the gene is expressed, but also that there<br />is a transcription variation along the life<br />cycle of the parasite. These two findings are<br />the starting point for further studies since<br />they tentatively suggest that this enzyme<br />could be involved in the protein turnover<br />that occurs during parasite encystation.<br />Although preliminary, this study is the first<br />report concerning the study of a specific<br />deubiquitinating enzyme in the parasite G.<br />intestinalis.

scholarly journals Complete Genome Analysis of a Novel Shewanella Phage vB_Sb_QDWS

2021 ◽  
Author(s):  
Lin Tan ◽  
Guanhua Xuan ◽  
Hong Lin ◽  
Jingxue Wang
Keyword(s):  
Complete Genome ◽  
Bioinformatic Analysis ◽  
Lytic Bacteriophage ◽  
Protein Coding ◽  
Putative Protein ◽  
Protein Coding Genes ◽  
Double Stranded Dna ◽  
The Family ◽  
Wastewater Samples ◽  
Complete Genome Analysis

Abstract We present here the results of the analysis of the complete genome sequence of a lytic bacteriophage, vB_Sb_QDWS, which is isolated from wastewater samples collected in Qingdao, China. The genome of phage vB_Sb_QDWS is composed of circular double-stranded DNA that is 47,902 bp in length with a G + C content of 63.16%. It has been predicted to contain 69 putative protein-coding genes. Phage morphology and bioinformatic analysis indicated that vB_Sb_QDWS is a novel phage of the family Siphoviridae.

TEORI KECERDASAN, PENDIDIKAN ANAK, DAN KOMUNIKASI DALAM KELUARGA

1970 ◽  
Vol 6 (1) ◽  
Author(s):  
Muskinul Fuad
Keyword(s):  
Multiple Intelligences ◽  
Educational Activity ◽  
Howard Gardner ◽  
Human Beings ◽  
Brain Functions ◽  
Starting Point ◽  
The Family ◽  
Education Today ◽  
The Brain ◽  
Spatial Logics

The education system in Indonesia emphasize on academic intelligence, whichincludes only two or three aspects, more than on the other aspects of intelligence. For thatreason, many children who are not good at academic intelligence, but have good potentials inother aspects of intelligence, do not develop optimally. They are often considered and labeledas "stupid children" by the existing system. This phenomenon is on the contrary to the theoryof multiple intelligences proposed by Howard Gardner, who argues that intelligence is theability to solve various problems in life and produce products or services that are useful invarious aspects of life.Human intelligence is a combination of various general and specific abilities. Thistheory is different from the concept of IQ (intelligence quotient) that involves only languageskills, mathematical, and spatial logics. According to Gardner, there are nine aspects ofintelligence and its potential indicators to be developed by each child born without a braindefect. What Gardner suggested can be considered as a starting point to a perspective thatevery child has a unique individual intelligence. Parents have to treat and educate theirchildren proportionally and equitably. This treatment will lead to a pattern of education that isfriendly to the brain and to the plurality of children’s potential.More than the above points, the notion that multiple intelligences do not just comefrom the brain needs to be followed. Humans actually have different immaterial (spiritual)aspects that do not refer to brain functions. The belief in spiritual aspects and its potentialsmeans that human beings have various capacities and they differ from physical capacities.This is what needs to be addressed from the perspective of education today. The philosophyand perspective on education of the educators, education stakeholders, and especially parents,are the first major issue to be addressed. With this step, every educational activity andcommunication within the family is expected to develop every aspect of children'sintelligence, especially the spiritual intelligence.

scholarly journals Sequencing of Organellar Genomes of Nowellia curvifolia (Cephaloziaceae Jungermanniales) Revealed the Smallest Plastome with Complete Gene Set and High Intraspecific Variation Suggesting Cryptic Speciation

Diversity ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 81
Author(s):  
Jakub Sawicki ◽  
Katarzyna Krawczyk ◽  
Monika Ślipiko ◽  
Monika Szczecińska
Keyword(s):  
Cryptic Speciation ◽  
Editing Event ◽  
Protein Coding ◽  
Gene Set ◽  
Protein Coding Genes ◽  
Organellar Genomes ◽  
The Family ◽  
Leafy Liverwort ◽  
Aneura Mirabilis ◽  
First Time

The leafy liverwort Nowellia curvifolia is a widespread Holarctic species belonging to the family Cephaloziaceae. It is made up of a newly sequenced, assembled and annotated organellar genomes of two European specimens, which revealed the structure typical for liverworts, but also provided new insights into its microevolution. The plastome of N. curvifolia is the second smallest among photosynthetic liverworts, with the shortest known inverted repeats. Moreover, it is the smallest liverwort genome with a complete gene set, since two smaller genomes of Aneura mirabilis and Cololejeunea lanciloba are missing six and four protein-coding genes respectively. The reduction of plastome size in leafy liverworts seems to be mainly impacted by deletion within specific region between psbA and psbD genes. The comparative intraspecific analysis revealed single SNPs difference among European individuals and a low number of 35 mutations differentiating European and North American specimens. However, the genetic resources of Asian specimen enabled to identify 1335 SNPs in plastic protein-coding genes suggesting an advanced cryptic speciation within N. curvifolia or the presence of undescribed morphospecies in Asia. Newly sequenced mitogenomes from European specimens revealed identical gene content and structure to previously published and low intercontinental differentiation limited to one substitution and three indels. The RNA-seq based RNA editing analysis revealed 17 and 127 edited sites in plastome and mitogenome respectively including one non-canonical editing event in plastid chiL gene. The U to C editing is common in non-seed plants, but in liverwort plastome is reported for the first time.

scholarly journals Sex-Biased lncRNA Signature in Fetal Growth Restriction (FGR)

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 921
Author(s):  
Aleksandra Lipka ◽  
Jan Pawel Jastrzebski ◽  
Lukasz Paukszto ◽  
Karol Gustaw Makowczenko ◽  
Elzbieta Lopienska-Biernat ◽  
...  
Keyword(s):  
Fetal Growth ◽  
Fetal Growth Restriction ◽  
Target Genes ◽  
Active Regions ◽  
Bioinformatic Analysis ◽  
Growth Restriction ◽  
Differentially Expressed ◽  
Circular Rnas ◽  
Differential Analysis ◽  
Protein Coding

Impaired fetal growth is one of the most important causes of prematurity, stillbirth and infant mortality. The pathogenesis of idiopathic fetal growth restriction (FGR) is poorly understood but is thought to be multifactorial and comprise a range of genetic causes. This research aimed to investigate non-coding RNAs (lncRNAs) in the placentas of male and female fetuses affected by FGR. RNA-Seq data were analyzed to detect lncRNAs, their potential target genes and circular RNAs (circRNAs); a differential analysis was also performed. The multilevel bioinformatic analysis enabled the detection of 23,137 placental lncRNAs and 4263 of them were classified as novel. In FGR-affected female fetuses’ placentas (ff-FGR), among 19 transcriptionally active regions (TARs), five differentially expressed lncRNAs (DELs) and 12 differentially expressed protein-coding genes (DEGs) were identified. Within 232 differentially expressed TARs identified in male fetuses (mf-FGR), 33 encompassed novel and 176 known lncRNAs, and 52 DEGs were upregulated, while 180 revealed decreased expression. In ff-FGR ACTA2-AS1, lncRNA expression was significantly correlated with five DEGs, and in mf-FGR, 25 TARs were associated with DELs correlated with 157 unique DEGs. Backsplicing circRNA processes were detected in the range of H19 lncRNA, in both ff- and mf-FGR placentas. The performed global lncRNAs characteristics in terms of fetal sex showed dysregulation of DELs, DEGs and circRNAs that may affect fetus growth and pregnancy outcomes. In female placentas, DELs and DEGs were associated mainly with the vasculature, while in male placentas, disturbed expression predominantly affected immune processes.

An endoscopic approach to the deviated nasal septum – a preliminary study

1998 ◽  
Vol 112 (10) ◽  
pp. 934-939 ◽  
Author(s):  
Dipak Ranjan Nayak ◽  
R. Balakrishnan ◽  
K. Deepak Murthy
Keyword(s):  
Nasal Obstruction ◽  
Nasal Septum ◽  
Objective Assessment ◽  
Subjective Assessment ◽  
Endoscopic Approach ◽  
Lateral Nasal Wall ◽  
Combined Approach ◽  
Deviated Nasal Septum ◽  
Nasal Endoscope ◽  
Preliminary Study

AbstractThe authors have used the nasal endoscope for the precise identification of pathological abnormalities of the nasal septum in relation to the lateral nasal wall including the osteo-meatal complex and in its ultraconservative management. The aim of the study was to compare the efficacies of endoscope-aided septoplasty (EAS) over traditional septoplasty (TS) in treating the pathological septum and turbinates, performed in 30 cases each. The subjective assessment was carried out by visual analogue scores and objective assessment by nasal endoscopy. This study demonstrates the superiority and limitations of the endoscopic approach in managing a deviated nasal septum and the turbinates. The endoscope-aided technique was found to be more effective in relieving the contact areas and nasal obstruction (p = ≤0.05). The authors advocate a combined approach – an endoscopic approach for inaccessible posterior deviation and the conservative traditional technique for accessible anterior deviation of the nasal septum.

scholarly journals WD40-repeat 47, a microtubule-associated protein, is essential for brain development and autophagy

2017 ◽  
Vol 114 (44) ◽  
pp. E9308-E9317 ◽  
Author(s):  
Meghna Kannan ◽  
Efil Bayam ◽  
Christel Wagner ◽  
Bruno Rinaldi ◽  
Perrine F. Kretz ◽  
...  
Keyword(s):  
Corpus Callosum ◽  
Brain Development ◽  
Brain Connectivity ◽  
Neuronal Morphology ◽  
Dependent Manner ◽  
Wd40 Repeat ◽  
Starting Point ◽  
Neuronal Regulation ◽  
The Family ◽  
Cervical Ganglion

The family of WD40-repeat (WDR) proteins is one of the largest in eukaryotes, but little is known about their function in brain development. Among 26 WDR genes assessed, we found 7 displaying a major impact in neuronal morphology when inactivated in mice. Remarkably, all seven genes showed corpus callosum defects, including thicker (Atg16l1, Coro1c, Dmxl2, and Herc1), thinner (Kif21b and Wdr89), or absent corpus callosum (Wdr47), revealing a common role for WDR genes in brain connectivity. We focused on the poorly studied WDR47 protein sharing structural homology with LIS1, which causes lissencephaly. In a dosage-dependent manner, mice lacking Wdr47 showed lethality, extensive fiber defects, microcephaly, thinner cortices, and sensory motor gating abnormalities. We showed that WDR47 shares functional characteristics with LIS1 and participates in key microtubule-mediated processes, including neural stem cell proliferation, radial migration, and growth cone dynamics. In absence of WDR47, the exhaustion of late cortical progenitors and the consequent decrease of neurogenesis together with the impaired survival of late-born neurons are likely yielding to the worsening of the microcephaly phenotype postnatally. Interestingly, the WDR47-specific C-terminal to LisH (CTLH) domain was associated with functions in autophagy described in mammals. Silencing WDR47 in hypothalamic GT1-7 neuronal cells and yeast models independently recapitulated these findings, showing conserved mechanisms. Finally, our data identified superior cervical ganglion-10 (SCG10) as an interacting partner of WDR47. Taken together, these results provide a starting point for studying the implications of WDR proteins in neuronal regulation of microtubules and autophagy.

Complete Mitochondrial Genome Sequence Analysis and Phylogenetic Location Determination of Hydropsyche Fryeri (Insecta: Trichoptera)

2021 ◽  
Author(s):  
Yimin Li ◽  
Honglin Qin ◽  
Xifa Zhong ◽  
Jingcai Huang ◽  
Yujun Wang ◽  
...  
Keyword(s):  
Mitochondrial Genome ◽  
Genome Sequence ◽  
Complete Mitochondrial Genome ◽  
Phylogenetic Tree Analysis ◽  
Protein Coding ◽  
Mitochondrial Genome Sequence ◽  
Complete Mitochondrial Genome Sequence ◽  
Location Determination ◽  
The Family

Abstract Hydropsyche fryeri belongs to the Trichopteridae family and builds nests in clean and unpolluted streams using stones. It also can be used as an indicator of water quality. Here, we describe the complete mitochondrial genome sequence of Hydropsyche fryeri. The mitochondrial genome is 15,676 bp long and contains 13 protein-coding genes, 22 tRNAs, 2 rRNAs and an AT-rich control region. Phylogenetic tree analysis shows that Hydropsyche fryeri is more closely related to the family Hydroptera than other Trichoptera.

scholarly journals Galls of Cecidoses eremita Curtis and Eucecidoses minutanus Brèthes (Lepidoptera: Cecidosidae) in Magdalena, Buenos Aires Province: preliminary study and associated fauna

2016 ◽  
Vol 16 (4) ◽  
Author(s):  
Veronica Loetti ◽  
Alejandra Valverde ◽  
Diana Nora Rubel
Keyword(s):  
Wall Thickness ◽  
Buenos Aires ◽  
Maximum Diameter ◽  
Buenos Aires Province ◽  
Associated Fauna ◽  
The Family ◽  
Preliminary Study

Abstract In Argentina, five galling species of the family Cecidosidae (Lepidoptera), including Cecidoses eremita Curtis and Eucecidoses minutanus Brèthes, have been cited. This note reports a preliminary study of their galls in "molles" (Schinus longifolius (Lindl.) Speg.) of Magdalena (Buenos Aires, Argentina). In April 2013, galls of C. eremita and E. minutanus were censused in randomly selected "molles". In April and December 2013, randomly selected galls were then collected and transported to our laboratory in hermetic bags, and conserved at -18 ºC until examination. Maximum diameter and wall thickness of collected galls were measured. About 84% of the observed "molles" (103/123) had galls of C. eremita and/or E. minutanus. The median of galls per tree was 12 (Q1=6; Q3=22). Eucecidoses minutanus had galls with smaller diameter (U11;53= 583; P<0.05) and thinner wall (U10;52=506.5; P<0..05) than C. eremita. In open galls, we found Pseudoescorpionida, Araneae (Segestriidae and Salticidae), and larvae of Lepidoptera and Hymenoptera. Within closed galls, we found adults of Torymidae and Chalcidoidea, and larvae of Ichneumonoidea.

scholarly journals Treatments’ Complexity in Dental Care Assistance, Urban Versus Rural Environment - Preliminary Study

2021 ◽  
Vol 26 (1) ◽  
pp. 75-78
Author(s):  
Camelia Ionescu ◽  
Mădălina Adriana Malița ◽  
Viorel Ștefan Perieanu ◽  
Mihai Burlibașa ◽  
Magdalena Natalia Dina ◽  
...  
Keyword(s):  
Dental Care ◽  
Urban Areas ◽  
Rural Environment ◽  
Facial Region ◽  
Starting Point ◽  
Preliminary Study ◽  
Dental Offices ◽  
Care Assistance ◽  
Urban Versus ◽  
Made In

Abstract When talking about dental assistance we actually refer to the prevention, detection and treatment of diseases of the oral and maxillo-facial region which, in most cases, have as a starting point or interest the dento-maxillary system. In this material, we tried to present a comparative study on the typology and complexity of different stages of dental treatments that can be performed in urban areas, compared to various stages of dental treatments that are performed and / or could be made in rural dental offices from in Romania.

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