scholarly journals Changes in antioxidant enzyme activities in Eichhornia crassipes (Pontederiaceae) and Pistia stratiotes (Araceae) under heavy metal stress

2007 ◽  
Vol 55 (3-4) ◽  
Author(s):  
V.J. Odjegba ◽  
I.O. Fasidi
2020 ◽  
Vol 73 (3) ◽  
Author(s):  
Amira Hassanein ◽  
Nihal Esmail ◽  
Hanan Hashem

This study was conducted to investigate the effects of sodium nitroprusside (SNP) on antioxidant enzyme activities in <em>Lupinus </em><em>albus </em>subsp. <em>termis </em>(Forssk.) Ponert plants subjected to salt and heavy metal stress. Foliar spray of SNP (0.4 and 0.6 mM) was used as a nitric oxide (NO) donor to treat lupine plants grown under different levels of salinity (0, 75, and 150 mM NaCl) and nickel (Ni) stress (100 and 150 mM Ni sulfate). Growth parameters and yield as well as total phenols, flavonoids, and antioxidant enzyme activities (including those of superoxide dismutase, peroxidase, ascorbate peroxidase, catalase, and glutathione transferase) in NO-treated and untreated plants grown under normal or salt/heavy metal  stress conditions were determined. We found that exogenously applied SNP effectively mitigated the inhibitory effects of salinity and Ni stresses on all measured growth parameters and yield components of lupine plants. In addition, NO downregulated antioxidant enzyme activities, which proved to be a good indicator reflecting changes in the oxidative status of lupine plants in response to SNP, salt, and Ni sulfate treatments.


2021 ◽  
Author(s):  
Ayushi Varshney ◽  
Praveen Dahiya ◽  
Sumedha Mohan

Abstract Phytoremediation of fly ash (FA) deposits using metal tolerant plant species has become an important eco-friendly technique for reclamation nowadays. The present study was carried out to determine the impact of FA application on photosynthetic pigments (chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids), biochemical parameters (soluble protein, reducing sugar, nitrate and nitrate reductase (NR) activity), metal accumulation (Fe, Zn, Cu, Co, Cd, Cr, and Mn) and antioxidant defense activity (SOD, CAT, POD, and APX) of Calendula officinalis. With this aim in mind, under pot culture conditions, Calendula officinalis was grown in different combinations of FA and soil which include: Control, 10%, 20%, 40%, 60%, 80%, and 100% FA. The results from the study indicated that the addition of FA (40%) in soil not only improved the physico-chemical properties of soil but also increased the photosynthetic pigment and other biochemical parameters in plants, however, these parameters declined under high FA applications. On the other hand, antioxidant enzyme activities (SOD, CAT, APX and Peroxidase) of Calendula increased with increasing FA application to combat heavy metal stress from fly ash. At high FA applications, antioxidant enzyme levels increased in leaves thereby reflecting heavy metal stress and mitigation of reactive oxygen species.


Author(s):  
Sevinc Kiran ◽  
Fatma Özkay ◽  
Kuşvuran Şebnem ◽  
Ellialtıoğlu Şebnem

In this study, it was aimed to demonstrate the effect of humic acid applications on some morphological, physiological and biochemical characteristics of eggplant genotypes (Burdur Merkez, Burdur Bucak, Kemer and Giresun) irrigated with the irrigation water with a high content of heavy metal and determined previously salt tolerance levels. In studies conducted in controlled greenhouse conditions, eggplant seeds germinated in the growth substrate a mixture of peat and perlite and the seedlings were transplanted into pots at 20 days after sowing. Plants when they are 4-5 true leaves, 3 different humic acid levels (0, 500, 1000 ppm) have been applied and 7 days later after this application began to be watered with 3 different irrigation water comprising a mixture of various doses of heavy metals (control: 0 ppm; I. Mixture: 0.2 ppm 5 ppm to 0.01 ppm Cd + Cu + Pb + 2 ppm Zn, II. mixture: +0.02 ppm 0.4 ppm Cu 10 ppm Pb + Cd + 4 ppm Zn). Field capacity level for the plants 40 days after quenched with water after which time they were harvested and samples for analysis were performed. In the study plants were investigated for shoot and root fresh weight, shoot and root dry weight, shoot and root length, leaf area, chlorophyll and MDA level of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzyme activities All genotypes are adversely affected by heavy metal applications. In parallel to increase the dose, heavy metal mixtures led to a reduction in values of fresh and dry weight of shoot and root, stem and root length, leaf area of eggplant genotypes. MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. Humic acid applications had a positive effect on reducing of the limiting effect of heavy metal stress on growth and development. As a result, compared to sensitive genotypes Giresun and Kemer, salt tolerant genotypes Burdur Merkez and Burdur Bucak showed much better resistance to abiotic stress factor which consists of heavy metal applications. The obtained results; formed also an opinion about that plants evolved similar strategies for resistance to abiotic stresses such as salinity, drought and heavy metal stress.


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