scholarly journals AğırMetal İçeriği Yüksek Sularla Sulanan Patlıcan Bitkilerine Uygulanan Humik Asidin Bazı Morfolojik, Fizyolojik ve Biyokimyasal ÖzelliklerÜzerine Etkisi

Author(s):  
Sevinc Kiran ◽  
Fatma Özkay ◽  
Kuşvuran Şebnem ◽  
Ellialtıoğlu Şebnem

In this study, it was aimed to demonstrate the effect of humic acid applications on some morphological, physiological and biochemical characteristics of eggplant genotypes (Burdur Merkez, Burdur Bucak, Kemer and Giresun) irrigated with the irrigation water with a high content of heavy metal and determined previously salt tolerance levels. In studies conducted in controlled greenhouse conditions, eggplant seeds germinated in the growth substrate a mixture of peat and perlite and the seedlings were transplanted into pots at 20 days after sowing. Plants when they are 4-5 true leaves, 3 different humic acid levels (0, 500, 1000 ppm) have been applied and 7 days later after this application began to be watered with 3 different irrigation water comprising a mixture of various doses of heavy metals (control: 0 ppm; I. Mixture: 0.2 ppm 5 ppm to 0.01 ppm Cd + Cu + Pb + 2 ppm Zn, II. mixture: +0.02 ppm 0.4 ppm Cu 10 ppm Pb + Cd + 4 ppm Zn). Field capacity level for the plants 40 days after quenched with water after which time they were harvested and samples for analysis were performed. In the study plants were investigated for shoot and root fresh weight, shoot and root dry weight, shoot and root length, leaf area, chlorophyll and MDA level of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzyme activities All genotypes are adversely affected by heavy metal applications. In parallel to increase the dose, heavy metal mixtures led to a reduction in values of fresh and dry weight of shoot and root, stem and root length, leaf area of eggplant genotypes. MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. Humic acid applications had a positive effect on reducing of the limiting effect of heavy metal stress on growth and development. As a result, compared to sensitive genotypes Giresun and Kemer, salt tolerant genotypes Burdur Merkez and Burdur Bucak showed much better resistance to abiotic stress factor which consists of heavy metal applications. The obtained results; formed also an opinion about that plants evolved similar strategies for resistance to abiotic stresses such as salinity, drought and heavy metal stress.

Author(s):  
Fatma Özkay ◽  
Sevinç Kıran ◽  
Şebnem Kuşvuran ◽  
Şeküre Şebnem Ellialtıoğlu

In this study was carried out to determine the effects of humic acid in heavy metal stress of lettuce changes in some of the morphological and physiological characteristics. In studies conducted in controlled greenhouse conditions, lettuce plants subjected to four different 4 different humic acid levels humic acid doses (0, 2, 4, 8 L/da) and 4 different heavy metal irrigation levels (Control: 0 ppm; I. mixture: 0.2 ppm Cu + 0.01 ppm Cd + 5 ppm Pb + 2 ppm Zn; II. mixture: : 0.4 ppm Cu + 0.02 ppm Cd + 10 ppm Pb + 4 ppm Zn; III. mixture: 0.8 ppm Cu + 0.04 ppm Cd + 20 ppm Pb + 8 ppm Zn). Four weeks after at heavy metal and humic acid treatment young plants were harvested and the shoot fresh and dry weight, root fresh and dry weight, shoot and root length, and leaf areas, MDA, superoxyde dismutase (SOD) and glutathione reductase (GR) were determined. The most highly toxic effects were determinate III. mixture level (0.8 ppm Cu + 0.04 ppm Cd + 20 ppm Pb + 8 ppm Zn). MDA and antioxidative enzyme activities increased in plants irrigated with water containing a mixture of heavy metal. Humic acid applications had a positive effect on reducing of the limiting effect of heavy metal stress on growth and development.


2020 ◽  
Vol 73 (3) ◽  
Author(s):  
Amira Hassanein ◽  
Nihal Esmail ◽  
Hanan Hashem

This study was conducted to investigate the effects of sodium nitroprusside (SNP) on antioxidant enzyme activities in <em>Lupinus </em><em>albus </em>subsp. <em>termis </em>(Forssk.) Ponert plants subjected to salt and heavy metal stress. Foliar spray of SNP (0.4 and 0.6 mM) was used as a nitric oxide (NO) donor to treat lupine plants grown under different levels of salinity (0, 75, and 150 mM NaCl) and nickel (Ni) stress (100 and 150 mM Ni sulfate). Growth parameters and yield as well as total phenols, flavonoids, and antioxidant enzyme activities (including those of superoxide dismutase, peroxidase, ascorbate peroxidase, catalase, and glutathione transferase) in NO-treated and untreated plants grown under normal or salt/heavy metal  stress conditions were determined. We found that exogenously applied SNP effectively mitigated the inhibitory effects of salinity and Ni stresses on all measured growth parameters and yield components of lupine plants. In addition, NO downregulated antioxidant enzyme activities, which proved to be a good indicator reflecting changes in the oxidative status of lupine plants in response to SNP, salt, and Ni sulfate treatments.


Author(s):  
Yibo Tang ◽  
Meiling Liu ◽  
Xiangnan Liu ◽  
Ling Wu ◽  
Bingyu Zhao ◽  
...  

Crops under various types of stresses, such as stress caused by heavy metals, drought and pest/disease exhibit similar changes in physiological-biochemical parameters (e.g., leaf area index [LAI] and chlorophyll). Thus, differentiating between heavy metal stress and nonheavy metal stress presents a great challenge. However, different stressors in crops do cause variations in spatiotemporal characteristics. This study aims to develop a spatiotemporal index based on LAI time series to identify heavy metal stress under complex stressors on a regional scale. The experimental area is located in Zhuzhou City, Hunan Province. The situ measured data and Sentinel-2A images from 2017 and 2018 were collected. First, a series of LAI in rice growth stages was simulated based on the WOrld FOod STudies (WOFOST) model incorporated with Sentinel 2 images. Second, the local Moran’s I and dynamic time warping (DTW) of LAI were calculated. Third, a stress index based on spatial and temporal features (SIST) was established to assess heavy metal stress levels according to the spatial autocorrelation and temporal dissimilarity of LAI. Results revealed the following: (1) The DTW of LAI is a good indicator for distinguishing stress levels. Specifically, rice subjected to high stress levels exhibits high DTW values. (2) Rice under heavy metal stress is well correlated with high-high SIST clusters. (3) Rice plants subjected to high pollution are observed in the northwest of the study regions and rice under low heavy metal stress is found in the south. The results suggest that SIST based on a sensitive indicator of rice biochemical impairment can be used to accurately detect regional heavy metal stress in rice. Combining spatial-temporal features and spectral information appears to be a highly promising method for discriminating heavy metal stress from complex stressors.


Agronomy ◽  
2021 ◽  
Vol 11 (9) ◽  
pp. 1820
Author(s):  
Nida Zainab ◽  
Amna ◽  
Amir Abdullah Khan ◽  
Muhammad Atif Azeem ◽  
Baber Ali ◽  
...  

The release of harmful wastes via different industrial activities is the main cause of heavy metal toxicity. The present study was conducted to assess the effects of heavy metal stress on the plant growth traits, antioxidant enzyme activities, chlorophyll content and proline content of Sesbania sesban with/without the inoculation of heavy-metal-tolerant Bacillus gibsonii and B. xiamenensis. Both PGP strains showed prominent ACC-deaminase, indole acetic acid, exopolysaccharides production and tolerance at different heavy metal concentrations (50–1000 mg/L). Further, in a pot experiment, S. sesban seeds were grown in contaminated and noncontaminated soils. After harvesting, plants were used for the further analysis of growth parameters. The experiment comprised of six different treatments. The effects of heavy metal stress and bacterial inoculation on the plant root length; shoot length; fresh and dry weight; photosynthetic pigments; proline content; antioxidant activity; and absorption of metals were observed at the end of the experiment. The results revealed that industrially contaminated soils distinctly reduced the growth of plants. However, both PGPR strains enhanced the root length up to 105% and 80%. The shoot length was increased by 133% and 75%, and the fresh weight was increased by 121% and 129%. The proline content and antioxidant enzymes posed dual effects on the plants growing in industrially contaminated soil, allowing them to cope with the metal stress, which enhanced the plant growth. The proline content was increased up to 190% and 179% by the inoculation of bacterial strains. Antioxidant enzymes, such as SOD, increased to about 216% and 245%, while POD increased up to 48% and 49%, respectively. The results clearly show that the utilized PGPR strains might be strong candidates to assist S. sesban growth under heavy metal stress conditions. We highly suggest these PGPR strains for further implementation in field experiments.


Agromix ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 218-226
Author(s):  
Nasrudin Nasrudin ◽  
Efrin Firmansyah

Drought stress affected growth and decreases rice yield. The use of high yielding varieties and drought tolerance is a strategic step that can be applied during drought stress. The aim of this research was to examine the response of the growth of IPB 4S rice varieties on planting media with drought stress during vegetative phase. The research used a nonfactorial Completely Randomized Design with six levels: field capacity, 10% of field capacity, 20% of field capacity, 30% of field capacity, 40% of field capacity, and 50% of field capacity. The treatments were repeated three times. The growth parameters were plant high, number of tiller, leaf color, leaf area, root length, root dry weight, canopy dry weight, and biomass. The results showed that the drought stress significantly different to leaf area, root length, canopy dry weight, and biomass but were not significantly different to plant height, number of tiller, leaf color, dan root dry weight. Increased of drought stress 30 to 50% of field capacity decreased of leaf area, canopy dry weight, and biomass, but increased root length.


2020 ◽  
pp. 1-12
Author(s):  
E. K. Al-Fahdawe ◽  
A. A. Al-Sumaidaie ◽  
Y. K. Al-Hadithy

A pots experiment was conducted at the Department of Biology/College of Education for Girls/University of Anbar during Autumn season of 2018-2019 to study the effect of the salinity irrigation water and spray by humic acid in some of morphological, physiological, growth and yield traits of wheat cv. IPa. The experiment was randomized complete block design (RCBD) with three replications. The first factor was assigned for irrigation by saline water at four level (S0, S1, S2 and S3), while the second factor was the foliar spraying of humic acid in three level (0.0, 1.0 and 1.5 g l-1). The results showed that there was significant reduction in plant height, vegetative dry weight, biological yield and chlorophyll leaves content when the plants were irrigated by saline water approached to 41.09 cm, 0.747 g, 0.849 g plant-1 and 38.67 SPAD, respectively at salinity level of 8.3 ds m-1 compared with the plants which irrigated by fresh water. The total carbohydrates were significantly decreased at the treatment of 8.3 ds m-1 reached 18.71 mg g-1. Spray levels humic acid achieved a significant increase in plant height, dry weight of the vegetative part, biological yield and chlorophyll leaves content sprayed at 1.0 and 1.5 g l-1 compared to no sprayed. Nitrogen concentration was significantly increased, while both phosphorus and potassium were decreased in the vegetative parts of wheat as the salinity of irrigation water increased. However, the increase of humic acid levels led to significant increasing in nitrogen, phosphorus and potassium concentration.


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