scholarly journals Prevalence of Mannheimia haemolytica in Syrian Awassi sheep

2019 ◽  
Vol 22 (4) ◽  
pp. 439-446 ◽  
Author(s):  
H. Al-haj Ali ◽  
B. Al Balaa
Keyword(s):  
Seasonal Distribution ◽  
Mannheimia Haemolytica ◽  
Awassi Sheep ◽  
Healthy Sheep ◽  
Nasal Swabs ◽  
Pcr Assays ◽  
Apparently Healthy

The aim of the present study was to evaluate the prevalence of Mannheimia haemolytica in Syrian Awassi sheep. Between 2008 and 2012, 1520 nasal swabs from pneumonic and apparently healthy sheep, and 110 pneumonic lungs samples were collected and subjected to bacteriological, biochemical and PCR assays. A total of 191 isolates (11.7%) were identified as M. haemolytica, 44 (2.7%) were M. ruminalis and 18 (1.1%) were M. glucosida. All 191 isolates of M. haemolytica gave an amplified product of 1146 bp size by PCR when lktaA primer was applied. The highest rate of M. haemolytica isolation was from pneumonic lungs tissue (21.8%) and pneumonic sheep (14.1%), and the lowest was from apparently healthy sheep (8.5%). Geographical and seasonal distribution of M. haemolytica showed that the majority of the isolates originated from sheep reared in the Syrian Desert (30%) and Euphrates basin (26.7%), and a significant increase (P≤0.05) in the rate of positive isolates in summer and winter as compared to spring. These findings indicate that M. haemolytica may play an important role in development of pneumonia in Syrian Awassi sheep, especially in eastern parts of Syria, where drought and shortage of rain hit these zones periodically.

scholarly journals Characterisation and pathogenicity of Pasteurella multocida capsular serogroup A isolates from Awassi sheep in Syria

2021 ◽  
Vol 24 (4) ◽  
pp. 487-496
Author(s):  
H. Al-haj Ali ◽  
B. Al Balaa
Keyword(s):  
Pasteurella Multocida ◽  
Pcr Assay ◽  
Local Breed ◽  
Fowl Cholera ◽  
Awassi Sheep ◽  
Biochemical Characterisation ◽  
Healthy Sheep ◽  
Nasal Swabs ◽  
Pathogenicity Tests ◽  
Apparently Healthy

The aim of the present study was to evaluate the prevalence of serogroup A of Pasteurella multocida in Syrian Awassi sheep. Of 1630 samples collected from nasal swabs of healthy and pneumonic sheep (125 herds) and pneumonic sheep lungs, a total of 228 (13.9%) strains were isolated and identified as P. multocida subsp. multocida by phenotypic and biochemical characterisation. However, of them only 117 (51.3%) were identified as serogroup A of P. multocida when PCR assay with specific primers for serogroup A strains was applied. The highest rate of serogroup A isolation was from apparently healthy sheep (49.6%) with consideration that all lung isolates (23 isolates) belonged to serogroup A. Geographical and seasonal distribution showed that about 60% of positively isolated bacteria originated from Syrian desert (29 isolates) and central parts of semi-arid step zone (41 isolates). A significant increase (P≤0.05) in the rate of positive isolates was observed in winter as compared to spring. Pathogenicity tests of 10 isolates with 50 or 10 LD50 values showed that 5 isolates were able to induce symptoms of fowl cholera in challenge-exposed chickens indicating that migratory Awassi sheep might serve as a carrier for serogroup A of P. multocida and that ovine isolates may be virulent for local breed of chickens.

Agglutinins to Common Salmonellae in the Sera of Apparently Healthy Sheep

1977 ◽  
Vol 133 (6) ◽  
pp. 615-622 ◽  
Author(s):  
W.J. Sojka ◽  
C. Wray ◽  
T.F. Brand
Keyword(s):  
Healthy Sheep ◽  
Apparently Healthy

scholarly journals Western Blot Analysis of the IgG-Antibody Response to Acid- Glycine-Extracted Antigens from Campylobacter fetus subsp. fetus and C. jejuni in Naturally Infected Sheep

2007 ◽  
Vol 76 (2) ◽  
pp. 245-251 ◽  
Author(s):  
K. Gürtürk ◽  
I. H. Ekin ◽  
A. Arslan
Keyword(s):  
Antibody Response ◽  
Western Blot ◽  
Blot Analysis ◽  
Western Blot Analysis ◽  
Igg Antibodies ◽  
Igg Antibody ◽  
Serological Tests ◽  
Recent Infection ◽  
Healthy Sheep ◽  
Apparently Healthy

IgG-antibody response in aborting sheep and in apparently healthy sheep in a flock against acidglycine- extracted antigens from three strains for each C. fetus subsp. fetus and C. jejuni were analysed by Western blot. One strain of C. fetus subsp. fetus was isolated from aborting sheep. Western blot analysis of the sera revealed the presence of IgG antibody binding to the common antigens including proteins with the Mw of 63 kDa and 54 kDa in extracts from both C. fetus subsp. fetus and C. jejuni strains. In addition, IgG antibodies in sera from aborting sheep reacted more strongly with the antigens from C. fetus subsp. fetus strains with Mw of approximately 100, 95 and 86.5 kDa than those of apparently healthy sheep. The binding profile of the antibodies with these antigens appeared to be unique for each C. fetus subsp. fetus strain. On the other hand, IgG antibodies only in sera from aborting sheep recognized strongly the antigens of each C. fetus subsp. fetus strain at the Mw ranged from approximately 26 to 22 kDa. However, the antigenic components between 26 and 22 kDa were not detectable in coomassie blue stained gel and thought to have non-protein nature. These low molecular weight antigens of C. fetus subsp. fetus may be related to a recent infection in aborting sheep. These observations indicate that such speciesspecific antigens or conjugated protein antigens could be used for improving the specificity of the serological tests to detect C. fetus antibodies in sheep sera, and may be the candidates for subunit vaccines against ovine abortion.

scholarly journals Erythrocyte potassium, sodium and glutathione concentrations and their relationship with reproduction, body weight and fleece weight traits in Awassi sheep

2008 ◽  
Vol 51 (5) ◽  
pp. 479-486
Author(s):  
N. Gunes ◽  
C. Aydin ◽  
C. Duygu Udum ◽  
S. Dikmen ◽  
O. Ozmen
Keyword(s):  
Body Weight ◽  
Milk Production ◽  
Birth Rate ◽  
Gene Frequencies ◽  
Twin Birth ◽  
Awassi Sheep ◽  
Healthy Sheep ◽  
Reproduction Traits ◽  
Erythrocyte Sodium ◽  
Fleece Weight

Abstract. Relationship between erythrocyte potassium (Ke), erythrocyte sodium (Nae) and blood glutathione (GSH) concentrations and production/ reproduction traits were investigated in Awassi sheep. Thirty healthy sheep subjected to analyze for phenotypic and gene frequencies of Ke, Nae and GSH concentrations. Correlation between these parameters and production/ reproduction traits such as greasy fleece weight, milk production, birth rate, single and twin birth rate and body weight were calculated. A positive correlation was found between birth rate and mean Ke concentration (r=0.481, P<0.008) and Nae concentration (r=−0.454, P<0.013). Another correlation between GSH concentration and greasy fleece weight (r=−0.368, P<0.049) and milk production (r=0.379, P<0.050) parameters was also evidenced. These suggest that Ke, Nae and GSH concentrations may be included in the metabolic profile testing parameters.

scholarly journals CULTURAL AND BIOCHEMICAL CHARACTERIZATION OF SHEEP Escherichia coli ISOLATED FROM IN AND AROUND BAU CAMPUS

1970 ◽  
Vol 8 (1) ◽  
pp. 51-55 ◽  
Author(s):  
M Purkayastha ◽  
MSR Khan ◽  
M Alam ◽  
MP Siddique ◽  
F Begum ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biochemical Characterization ◽  
Antibiotic Sensitivity ◽  
Resistance Pattern ◽  
E Coli ◽  
Bright Pink ◽  
Healthy Sheep ◽  
Metallic Sheen ◽  
Apparently Healthy

Pathogenic Escherichia coli remain as an important etiological agent of sheep diarrhoea in Bangladesh. The present study was designed for the cultural and biochemical characterization of Sheep E. coli from diarrhoeic and apparently healthy sheep in and around BAU campus for the period from January to October, 2007. Out of 90 faecal samples, 36 from diarrhoeic and 54 from apparently healthy sheep collected from different areas in and around BAU campus, 15 (41.67%) and 21 (38.38%) were found to be positive for E. coli. The cultural characterization of all positive sheep E. coli revealed greenish black colony with metallic sheen in Eosine methylene blue agar, bright pink color smooth transparent colony in MacConkey agar, green color colony in Brilliant green agar, slight pinkish smooth colony in Salmonella-Shigella agar and colorless colony with hemolysis in blood agar. In case of biochemical characterization, all of the isolates showed fermentation of dextrose, sucrose, fructose, maltose and mannitol with the production of acid and gas, negative result to Voges-Proskaure test, positive result to Methyl-red test and differential result to Indole test.The overall prevalence of E. coli was recorded as 80.05% through the cultural and biochemical characterization. The antibiotic sensitivity and resistance pattern showed that the isolates of sheep E. coli were highly sensitive to ciprofloxacine, co-trimoxazol, nalidixic acid and chloramphenicol but to the erythromycin the isolates were highly resistant. DOI = 10.3329/bjvm.v8i1.8350 Bangl. J. Vet. Med. (2010). 8(1): 51-55

scholarly journals Serological and Molecular Investigation of Batai Virus Infections in Ruminants from the State of Saxony-Anhalt, Germany, 2018

Viruses ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 370
Author(s):  
Nicole Cichon ◽  
Martin Eiden ◽  
Jana Schulz ◽  
Anne Günther ◽  
Patrick Wysocki ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Serological Test ◽  
Harbor Seal ◽  
Virus Infections ◽  
Eastern Germany ◽  
Ruminant Species ◽  
Healthy Sheep ◽  
Batai Virus ◽  
Apparently Healthy

Arthropod-borne Batai virus (BATV) is an Orthobunyavirus widely distributed throughout European livestock and has, in the past, been linked to febrile diseases in humans. In Germany, BATV was found in mosquitoes and in one captive harbor seal, and antibodies were recently detected in various ruminant species. We have, therefore, conducted a follow-up study in ruminants from Saxony-Anhalt, the most affected region in Eastern Germany. A total of 325 blood samples from apparently healthy sheep, goats, and cattle were tested using a BATV-specific qRT-PCR and SNT. Even though viral RNA was not detected, the presence of antibodies was confirmed in the sera of all three species: sheep (16.5%), goats (18.3%), and cattle (41.4%). Sera were further analyzed by a glycoprotein Gc-based indirect ELISA to evaluate Gc-derived antibodies as a basis for a new serological test for BATV infections. Interestingly, the presence of neutralizing antibodies was not directly linked to the presence of BATV Gc antibodies. Overall, our results illustrate the high frequency of BATV infections in ruminants in Eastern Germany.

scholarly journals Isolation, identification and characterization of bacterial flora from the respiratory tract of apparently healthy sheep

2016 ◽  
Vol 1 (3) ◽  
pp. 677-685 ◽  
Author(s):  
Chandan Kumar Sarker ◽  
Anowar Hossen ◽  
Md Abu Yousuf ◽  
Md Ala Uddin ◽  
Most Sumona Akter ◽  
...  
Keyword(s):  
Respiratory Tract ◽  
Bacterial Flora ◽  
Experimental Models ◽  
Upper Respiratory Tract ◽  
Biochemical Tests ◽  
Antibiotic Drug ◽  
Healthy Sheep ◽  
Identification And Characterization ◽  
Apparently Healthy

Sheep is the common name for a group of grazing mammals that may be either wild or domesticated; the domesticated varieties are amongst the most widely distributed types of domestic animal, found in nearly all countries. Bangladesh is a densely populated developing country and its economy is primarily based on agriculture. The current study was designed for isolation, identification and characterization of bacterial flora from the upper respiratory tract of sheep. Thirty (30) apparently healthy sheep were selected at the adjacent areas of Bangladesh Agricultural University (BAU) for this experiment. Swab samples were collected from nasal swabs (10), lung swabs (10) and tracheal swabs (10). All samples were subjected into inoculated on to bacteriological media (nutrient broth, nutrient agar, Salmonella-Shigella agar, MacConkey agar, blood agar, brilliant green agar). Furthermore, all of the bacterial isolates were characterized by Gram’s staining, biochemical tests (sugar fermentation tests, catalase test, coagulase test, indole test, MR-VP test), antibiotics sensitivity tests and pathogenicity tests. None of the isolated Bacillus, E. coli and Staphylococcus spp. was found to be pathogenic. Isolated Pasteurella spp. were found to be pathogenic as observed in different experimental models and showed a degree of variation in antibiotic drug sensitivity test. Ciprofloxacillin was sensitive to all of the isolated bacteria. Through the bacteria that were isolated from various organs of apparently healthy sheep is normal micro flora, however these may act as primary pathogen and may produce diseases when the sheep are immunologically suppressed due to severe stress conditions.Asian J. Med. Biol. Res. December 2015, 1(3): 677-685

scholarly journals Infectious causes of equine respiratory disease on Ontario standardbred racetracks

1977 ◽  
Vol 5 (3) ◽  
pp. 285-289
Author(s):  
J Sherman ◽  
J Thorsen ◽  
D A Barnum ◽  
W R Mitchell ◽  
D G Ingram
Keyword(s):  
Respiratory Disease ◽  
Influenza Viruses ◽  
Late Winter ◽  
Equine Herpesvirus ◽  
Serum Samples ◽  
Equine Herpesvirus 1 ◽  
Nasal Swabs ◽  
Upper Respiratory ◽  
Herpesvirus 1 ◽  
Apparently Healthy

Upper respiratory disease has been a serious problem in Standardbred horses on racetracks in Ontario, with outbreaks occurring once or twice annually in late winter and early spring seasons. To determine the causes of these epidemics, a 3-year investigation was carried out in which nasal swabs and serum samples were obtained at intervals from apparently healthy horses and from horses suffering from upper respiratory disease. The nasal swabs were used to isolate bacteria and viruses. The serum samples were examined for the presence and level of antibodies to equine influenza viruses and equine herpesvirus 1. None of the bacteria isolated were associated with the outbreaks of disease. Equine herpesvirus 2 was isolated 72 times from both diseased and apparently healthy horses. Equine herpesvirus 1 was isolated 10 times from horses with respiratory disease, both during and between epidemics. Influenza equine/1 virus was isolated seven times and influenza equine/2 was isolated once during severe outbreaks of upper respiratory disease. Serological evidence confirmed that influenza viruses were the causes of the major epidemics, with the equine/1 strain being involved most often.

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